公开(公告)号：US20150151301A1

公开(公告)日：2015-06-04

申请号：US14547737

申请日：2014-11-19

Applicant: IMEC VZW

Inventor： Paolo Fiorini ,  Tim Stakenborg ,  Frederik Colle

IPC: B01L3/00 ,  C12Q1/68 ,  B01L7/00

CPC classification number: B01L3/502784 ,  B01L7/52 ,  B01L2200/061 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/1827 ,  C12Q1/686

Abstract: A micro-fluidic device 100 for performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel 104, comprising an inlet 102 and an outlet 103, embedded in the semiconductor substrate; a heating element 101 thermally coupled to the first micro-fluidic channel 104; a droplet generator 107 connected to the inlet 102 of the first micro-fluidic channel 104 for generating droplets and pumping generated droplets at a flow rate into the first micro-fluidic channel 104; characterized in that: the heating element 101 is a single heating element connected to a temperature control unit 111 configured to cycle the temperature of the complete first micro-fluidic channel 104 through at least two temperature values; and wherein the flow rate of the droplet generator 107 is adaptable. Further, a method to perform digital PCR is presented using the micro-fluidic device 100.

Abstract translation: 提出了一种用于执行数字PCR的微流体装置100。 该器件包括：半导体衬底; 第一微流体通道104，包括嵌入在半导体衬底中的入口102和出口103; 热耦合到第一微流体通道104的加热元件101; 液滴发生器107，其连接到第一微流体通道104的入口102，用于产生液滴并以流速泵送产生的液滴到第一微流体通道104中; 其特征在于：加热元件101是连接到温度控制单元111的单个加热元件，温度控制单元111被配置为使完整的第一微流体通道104的温度循环至少两个温度值; 并且其中液滴发生器107的流量适应。 此外，使用微流体装置100呈现执行数字PCR的方法。

公开(公告)号：US20250114793A1

公开(公告)日：2025-04-10

申请号：US18909640

申请日：2024-10-08

Applicant: IMEC VZW

Inventor： Christopher Adams ,  Peter Peumans ,  Andy Lambrechts ,  Murali Jayapala ,  Tim Stakenborg

IPC: B01L3/00 ,  G01N15/1429

Abstract: A system for label-free identification and/or classification and/or selection of at least one cell is provided. The system comprises a microfluidic environment configured to be traversable by the at least one cell, an imaging sensor configured to gather imaging information with respect to at least a part of the microfluidic environment, and a processing unit connected to the imaging sensor. In this context, the microfluidic environment comprises at least one sub-environment configured to interact with the at least one cell. Furthermore, the processing unit is configured to detect at least one sub-environment interaction event based on persistence of the at least one cell imaged by the imaging sensor in the temporal domain. In addition to this, the processing unit is configured to identify and/or classify and/or select the at least one cell based on the at least one sub-environment interaction event.

公开(公告)号：US20250066817A1

公开(公告)日：2025-02-27

申请号：US18813902

申请日：2024-08-23

Applicant: IMEC VZW

Inventor： Koen Johannes Hubertus Gerardus de Wijs ,  Maarten Fauvart ,  Chengxun Liu ,  Tim Stakenborg

IPC: C12N15/87 ,  C12M1/42

Abstract: A method and system for electroporation of a biological cell in a fluidic channel is provided. The method includes: introducing a fluidic flow in a first direction in the fluidic channel wherein the fluidic flow comprises the cell; applying a first electrical field to the cell in the fluidic flow to produce a first electroporation at a first position in the fluidic channel; measuring an impedance of the cell in the fluidic flow at a second position in the fluidic channel; determining a second electrical field depending on the impedance measurement of the cell in the fluidic flow at the second position in the fluidic channel; and applying the second electrical field to the cell in the fluidic flow to produce a second electroporation at a third position in the fluidic channel, wherein the first, second and third positions are sequentially arranged in the first direction of the fluidic flow.

公开(公告)号：US11313489B2

公开(公告)日：2022-04-26

申请号：US17135192

申请日：2020-12-28

Applicant: IMEC VZW

Inventor： Lei Zhang ,  Tim Stakenborg ,  Chengxun Liu ,  David Cheyns

IPC: F16K31/128 ,  F16K7/10 ,  F16K7/12 ,  F16K31/00 ,  F16K11/24

Abstract: Example embodiments relate to microfluidic devices for controlling pneumatic microvalves. One embodiment includes a microfluidic device for independently controlling a plurality of pneumatic microvalves. The microfluidic device is couplable to a pressure source. The microfluidic device includes a first substrate. The microfluidic device also includes a flexible membrane covering the first substrate. Additionally, the microfluidic device includes a second substrate covering the flexible membrane. Further, the microfluidic device includes one or more fluidic channels at least partially defined in the first substrate. In addition, the microfluidic device includes a pressure couplable to the pressure source and branching into a plurality of pressure channels. Still further, the microfluidic device includes at least one pressure control switch per pressure channel.

公开(公告)号：US20220091117A1

公开(公告)日：2022-03-24

申请号：US17540551

申请日：2021-12-02

Applicant: Imec vzw

Inventor： Willem Van Roy ,  Tim Stakenborg ,  Kris Covens

IPC: G01N33/543 ,  C12Q1/00 ,  C12Q1/6825 ,  G01N33/52

Abstract: A device (1) for sensing an analyte, the device (1) comprises at least a sample inlet (10) for receiving a sample, affinity probes (111) selected to have a preferential binding to the analyte, a transducer (11) sensitive to a characteristic of the analyte and/or a label attached to the analyte, the transducer not being a FET transducer, and a desalting unit (13) for desalting the received sample.

公开(公告)号：US20220008917A1

公开(公告)日：2022-01-13

申请号：US17475807

申请日：2021-09-15

Applicant: Imec vzw

Inventor： Benjamin Jones ,  Tim Stakenborg ,  Paolo Fiorini

IPC: B01L3/00 ,  F16K99/00

Abstract: A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

公开(公告)号：US10928305B2

公开(公告)日：2021-02-23

申请号：US15736399

申请日：2016-06-30

Applicant: IMEC VZW

Inventor： Peter Peumans ,  Liesbet Lagae ,  Willem Van Roy ,  Tim Stakenborg ,  Pol Van Dorpe

IPC: G01N21/17 ,  G01N21/64

Abstract: A sensor device for quantifying luminescent targets comprises a light source, a detector, a modulator, and a processor. The light source is adapted for exciting the luminescent target. The detector is adapted for detecting the luminescence of the luminescent target resulting in a measured signal which comprises a desired signal originating from the luminescent target and a background signal. The modulator is adapted for modulating a physical parameter resulting in a modulation of the desired signal which is different from the modulation of the background signal. The processor is configured to correlate the modulation of the physical parameter with the modulation of the desired signal and/or the modulation of the background signal.

公开(公告)号：US20190355964A1

公开(公告)日：2019-11-21

申请号：US16411811

申请日：2019-05-14

Applicant: IMEC vzw

Inventor： Philippe Vereecken ,  Brecht Put ,  Tim Stakenborg ,  Arnaud Furnemont ,  Luca Di Plazza

IPC: H01M4/04 ,  C01G23/047 ,  C07H21/00 ,  H01B1/12 ,  H01M4/131

Abstract: A device for synthesis of macromolecules is disclosed. In one aspect, the device comprises an ion-releaser having a synthesis surface comprising an array of synthesis locations arranged for synthesis of the macromolecules. The ion-releaser also includes an ion-source electrode, which is arranged to contain releasable ions and is arranged to be in contact with each of the synthesis locations of the synthesis surface, thereby release ions to the synthesis locations. The ion-releaser further comprises activating electrodes, which are arranged to be in contact with the ion-source electrode, wherein each one of the activating electrodes is arranged in association with one of the synthesis locations via the ion-source electrode. The ion-releaser is arranged to release at least a portion of the releasable ions from the ion-source electrode to one of the synthesis locations, by activation of the activating electrode associated with the synthesis location.

公开(公告)号：US20190309346A1

公开(公告)日：2019-10-10

申请号：US16448713

申请日：2019-06-21

Applicant: IMEC VZW

Inventor： Tim Stakenborg ,  Paolo Fiorini

IPC: C12Q1/6806 ,  B01L3/00 ,  B01L7/00

Abstract: The disclosure provides methods and systems for analyzing fluid samples comprising obtaining fluid samples in at least one cavity of a substrate and introducing also buffers and/or reagents in the cavity, performing nucleic acid extraction and/or purification in the cavity, and performing nucleic acid amplification in the same cavity.

公开(公告)号：US20190291106A1

公开(公告)日：2019-09-26

申请号：US16363926

申请日：2019-03-25

Applicant: IMEC vzw

Inventor： Karolien Jans ,  Tim Stakenborg ,  Frank Holsteyns

IPC: B01L3/00 ,  G11C13/00

Abstract: The disclosed technology generally relates to storing data, and more particularly relates to a method of storing data in a polymer, where the data comprises a sequence of bits. In one aspect, the method comprises receiving a sequence of bits to be stored and providing a group of different homo-bifunctional monomers. Each homo-bifunctional monomer comprises a core structure having identical functional groups attached at two different positions of the core structure. The group of different homo-bifunctional monomers comprises homo-bifunctional monomers having at least two different core structures. The method further comprises linking the different homo-bifunctional monomers together to form the polymer having a sequence of monomer core structures representing the sequence of bits to be stored. The different homo-bifunctional monomers are linked together using a click chemistry reaction between the functional groups of the different homo-bifunctional monomers. The present disclosure further relates to a synthesis system for performing the method.