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21.发明授权公开(公告)号：US09568478B2
公开(公告)日：2017-02-14
申请号：US13751030
申请日：2013-01-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Levente Egry , Kyle Gee
CPC classification number: G01N33/6803 , C07F5/022 , C09B23/04 , C09B57/00 , G01N33/52 , G01N33/582 , G01N33/68
Abstract: According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.
Abstract translation: 根据本教导，提供了利用作为二吡咯亚甲基二氟化物化合物的染料的蛋白质熔融分析的系统，组合物，试剂盒和方法。 在一些实施方案中，一种方法包括通过将至少一种蛋白质与染料混合并在记录样品的荧光发射的同时施加受控加热来制备样品。
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公开(公告)号：US20150241438A1
公开(公告)日：2015-08-27
申请号：US14402375
申请日：2013-03-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle Gee , Upinder Singh , Aleksey Rukavishnikov , Daniel Beacham , Shih-Jung Huang , Michael Janes , Wenjun Zhou , Judith Berlier
IPC: G01N33/58 , C07D311/90
CPC classification number: C09B11/24 , A61K49/0041 , C07D311/90 , G01N33/5005 , G01N33/582 , G01N33/84
Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。
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公开(公告)号：US20140377837A1
公开(公告)日：2014-12-25
申请号：US14299284
申请日：2014-06-09
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian AGNEW , Kyle Gee , Schuyler Corry
IPC: G01N33/58
CPC classification number: C12P21/005 , A61K47/549 , C07K1/13 , C07K14/47 , C07K16/00 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C12Y302/01096 , G01N33/5005 , G01N33/5008 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/534 , G01N33/581 , G01N33/582 , G01N33/583 , Y10T436/17
Abstract: The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.
Abstract translation: 本发明通常涉及在寡糖键上官能化蛋白质，特别是抗体的方法，通过修饰糖基化来人源化抗体的方法，以及与修饰的寡糖连接的新型抗体。 本发明还涉及可用于产生本发明抗体的试剂盒。
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公开(公告)号：US20130288911A1
公开(公告)日：2013-10-31
申请号：US13793517
申请日：2013-03-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Aimei Chen , Kyle Gee , Hee Chol Kang
IPC: G01N21/64
CPC classification number: G01N33/64 , C07D311/82 , C07D471/06 , C07F5/022 , C07F9/5442 , C07F9/65522 , C09B11/24 , C09K11/06 , C09K2211/10 , C09K2211/1007 , C09K2211/1014 , C09K2211/1022 , C09K2211/1033 , C09K2211/1055 , C09K2211/1088 , G01N21/6428 , G01N21/6486 , G01N33/50 , G01N33/52 , G01N2021/6439 , Y10T436/143333
Abstract: The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ.
Abstract translation: 本公开涉及能够检测含有醛和酮基的分析物的荧光席夫碱形成染料。 染料含有亲核肼基附属物，并能够原位结合和检测分析物。
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公开(公告)号：US11865191B2
公开(公告)日：2024-01-09
申请号：US16882271
申请日：2020-05-22
Applicant: Life Technologies Corporation , Pierce Biotechnology, Inc.
Inventor： Surbhi Desai , Marie Christine Nlend , Kyle Gee , Matthew Baker , Robert Aggeler , Scott Sweeney , Aleksey Rukavishnikov , Shih-Jung Huang
CPC classification number: A61K49/0015 , A61K47/10 , A61K47/18 , A61K49/0058 , G01N33/533 , G01N33/582 , G01N33/68
Abstract: This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.
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Patent Agency Ranking
公开(公告)号：US20220026437A1
公开(公告)日：2022-01-27
申请号：US17492959
申请日：2021-10-04
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Levente Egry , Kyle Gee
Abstract: According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.
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公开(公告)号：US20210041465A1
公开(公告)日：2021-02-11
申请号：US17027391
申请日：2020-09-21
Applicant: Life Technologies Corporation
Inventor： Jeffrey DZUBAY , Kyle Gee , Vladimir Martin , Aleksey Rukavishnikov , Daniel Beacham
IPC: G01N33/84 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07D209/14 , C09K11/06 , C07F5/02 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/28 , C09B57/00 , C09B69/00 , G01N31/22 , G01N33/50 , C09B11/24 , G01N33/58 , G01N33/80
Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.
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公开(公告)号：US10676771B2
公开(公告)日：2020-06-09
申请号：US15385340
申请日：2016-12-20
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian Agnew , Kyle Gee , Schuyler Corry
IPC: C07K16/00 , C07K14/47 , G01N33/533 , C12P21/00 , A61K47/54 , G01N33/531 , C07K1/13 , G01N33/532 , G01N33/50 , G01N33/58 , G01N33/534
Abstract: The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.
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公开(公告)号：US20180362766A1
公开(公告)日：2018-12-20
申请号：US15955332
申请日：2018-04-17
Applicant: Life Technologies Corporation
Inventor： Kyle Gee , Upinder Singh , Aleksey Rukavishnikov , Daniel Beacham , Shih-Jung Huang , Michael Janes , Wenjun Zhou , Judith Berlier
Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
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公开(公告)号：US20180238912A1
公开(公告)日：2018-08-23
申请号：US15899021
申请日：2018-02-19
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jeffrey DZUBAY , Kyle Gee , Vladimir Martin , Aleksey Rukavishnikov , Daniel Beacham
IPC: G01N33/84 , C09B11/24 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/28 , C09B57/00 , G01N31/22 , G01N33/50 , G01N33/58 , C09K11/06 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07D209/14 , C07F5/02 , C09B69/00 , G01N33/80
CPC classification number: G01N33/84 , C07D209/14 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07F5/02 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/24 , C09B11/28 , C09B57/00 , C09B69/00 , C09K11/06 , G01N31/22 , G01N33/5005 , G01N33/5058 , G01N33/5091 , G01N33/582 , G01N33/80 , G01N2333/245 , Y10T436/143333
Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.
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