公开(公告)号：US20160024512A1

公开(公告)日：2016-01-28

申请号：US14862734

申请日：2015-09-23

Applicant: Monsanto Technology LLC

Inventor： Toni Armstrong ,  Kenneth Barton ,  Laura J. Crow ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Yong Huang ,  Brian J. Martinell ,  Michael W. Petersen ,  Kenneth J. Piller ,  Qi Wang ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8209 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8289

Abstract: The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

Abstract translation: 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因，阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体，将植物细胞再生成植物并鉴定独立的转基因基因座，其中可选择标记基因或转基因元件可以在后代中分离。

公开(公告)号：US09119408B2

公开(公告)日：2015-09-01

申请号：US13914505

申请日：2013-06-10

Applicant: Monsanto Technology LLC

Inventor： Michael H. Luethy ,  Thomas M. Malvar ,  Shihshieh Huang

IPC: C12N15/82 ,  A01H1/00 ,  A23K1/14 ,  C07K14/415 ,  C12N9/06 ,  A23L1/10

Abstract: Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.

公开(公告)号：US20130074213A1

公开(公告)日：2013-03-21

申请号：US13677607

申请日：2012-11-15

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Edwards Allen ,  Larry A. Gilbertson ,  James K. Roberts ,  Nancy M. Houmard ,  Shihshieh Huang ,  Sergey Ivashuta

IPC: C12N15/82 ,  A01H1/02 ,  A01H5/10 ,  A01H5/00

CPC classification number: C12N15/8218 ,  A01H1/02 ,  A01H5/00 ,  A01H5/10 ,  C12N15/8287 ,  C12N15/8289 ,  C12N15/829

Abstract: This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.

Abstract translation: 本发明提供了生产非天然杂种种子的方法。 还公开了可用于在作物植物上赋予诱导型不育性的特异性miRNA和miRNA识别位点，以及包含这种外源miRNA识别位点的重组DNA构建体。

公开(公告)号：US10883103B2

公开(公告)日：2021-01-05

申请号：US15579125

申请日：2016-06-02

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Michael J. Bennett ,  Shihshieh Huang ,  Alberto B. Iandolino ,  Heather A. Lindfors ,  Janette V. Oakes ,  Gregory John Peel ,  Sharon E. Radke ,  Steven H. Schwartz

IPC: C12N15/87 ,  C12N15/113 ,  C12N15/82 ,  C12N9/22

Abstract: The present disclosure provides compositions and methods for delivering a polynucleotide from the exterior surface of a plant or plant part into the interior of a plant cell. More specifically, the present disclosure relates to compositions comprising at least one polynucleotide and at least one agent that is able to disrupt at least one barrier of the plant or plant part.

公开(公告)号：US10435686B2

公开(公告)日：2019-10-08

申请号：US14567786

申请日：2014-12-11

Applicant: Monsanto Technology LLC

Inventor： Edwards M. Allen ,  Barry S. Goldman ,  Liang Guo ,  Sara E. Heisel ,  Shihshieh Huang ,  Sergey I. Ivashuta ,  David K. Kovalic ,  Elysia K. Krieger ,  James K. Roberts ,  Yuanji Zhang

IPC: C12N15/113 ,  C12N15/82

Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.

公开(公告)号：US20180007856A9

公开(公告)日：2018-01-11

申请号：US13914505

申请日：2013-06-10

Applicant: Monsanto Technology LLC

Inventor： Michael H. Luethy ,  Thomas M. Malvar ,  Shihshieh Huang

IPC: A01H5/10 ,  C12N9/06 ,  C07K14/415 ,  C12N15/82

CPC classification number: A01H5/10 ,  A23K10/30 ,  A23L7/198 ,  C07K14/415 ,  C12N9/0028 ,  C12N15/8218 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8254

Abstract: Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.

公开(公告)号：US20170342426A1

公开(公告)日：2017-11-30

申请号：US15670668

申请日：2017-08-07

Applicant: Monsanto Technology LLC

Inventor： Toni Armstrong ,  Kenneth Barton ,  Laura J. Crow ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Yong Huang ,  Brian J. Martinell ,  Michael W. Petersen ,  Kenneth J. Piller ,  Qi Wang ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8209 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8289

Abstract: The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

公开(公告)号：US09670502B2

公开(公告)日：2017-06-06

申请号：US13750971

申请日：2013-01-25

Applicant: Monsanto Technology LLC

Inventor： Shihshieh Huang ,  Stanislaw Flasinski ,  Alessandra Frizzi ,  Brad Gabor ,  Charles Hagen ,  John Kao ,  Raquel Salati

IPC: C12N15/82

CPC classification number: C12N15/8283 ,  C12N15/8218

Abstract: The present invention provides gene targets, constructs and methods for the genetic control of plant disease caused by multiple plant viruses. The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences of plant-parasitic viruses. Protein-expression based approaches may also be utilized to augment phenotype resistance. Thus, transcription of a single transgenic event comprising one or more plant expression cassettes can allow for broad spectrum resistance of a plant to multiple plant viral strains and species among the geminiviruses, tospoviruses, and potexviruses.

公开(公告)号：US20160032300A1

公开(公告)日：2016-02-04

申请号：US14853715

申请日：2015-09-14

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  Larry A. Gilbertson ,  James K. Roberts ,  Nancy M. Houmard ,  Shihshieh Huang ,  Sergey Ivashuta

IPC: C12N15/82

CPC classification number: C12N15/8218 ,  A01H1/02 ,  A01H5/00 ,  A01H5/10 ,  C12N15/8287 ,  C12N15/8289 ,  C12N15/829

Abstract: This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.

公开(公告)号：US20150184179A1

公开(公告)日：2015-07-02

申请号：US14582303

申请日：2014-12-24

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Edwards M. Allen ,  Larry A. Gilbertson ,  Sara E. Heisel ,  Shihshieh Huang ,  Elysia K. Krieger ,  Thomas M. Malvar

IPC: C12N15/82 ,  C12N15/113

CPC classification number: C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

Abstract translation: 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。