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公开(公告)号：US10808257B2
公开(公告)日：2020-10-20
申请号：US15843823
申请日：2017-12-15
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Larry A. Gilbertson , Shihshieh Huang , Thomas Malvar
IPC: C12N15/82
Abstract: Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.
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公开(公告)号：US10787680B2
公开(公告)日：2020-09-29
申请号：US16716281
申请日：2019-12-16
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. Larosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
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公开(公告)号：US10435698B2
公开(公告)日：2019-10-08
申请号：US15616881
申请日：2017-06-07
Applicant: Monsanto Technology LLC
Inventor： Larry A. Gilbertson , Susan J. Johnson
IPC: C12N15/84 , C12N15/113 , A01H1/04 , C12N15/82
Abstract: Methods of, and compositions for, assembling one or more transcription units in a genome without a linked selectable marker or other unwanted transcription unit are provided. Also provided methods of, and compositions for, assembling one or more transcription units in a genome with a reduced frequency of vector backbone.
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公开(公告)号：US20170107527A1
公开(公告)日：2017-04-20
申请号：US15177576
申请日：2016-06-09
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Stanton B. Dotson , Larry A. Gilbertson , Jonathan C. Lamb , Brenda A. Lowe , Zoe P. McCuddin
IPC: C12N15/82
CPC classification number: C12N15/8213 , C12N15/8241 , C12N15/8243 , C12N15/8245 , C12N15/8247 , C12N15/8251 , C12N15/8257 , C12N15/8261 , C12N15/827 , C12N15/8271 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12N15/8289 , C12N15/829
Abstract: The invention provides novel uses of sequence-specific or sequence-directed endonucleases for molecular plant breeding. The invention also provides novel plant transformation vectors and expression cassettes, which include novel combinations of an endonuclease with plant expression and transformation elements. Plants and derivatives thereof produced by such methods are also provided.
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25.发明授权公开(公告)号：US09464295B2
公开(公告)日：2016-10-11
申请号：US14295096
申请日：2014-06-03
Applicant: Monsanto Technology LLC
Inventor： Larry A. Gilbertson , Xudong Ye
CPC classification number: C12N15/8205 , C12N15/8202
Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.
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Patent Agency Ranking
26.发明申请公开(公告)号：US20160068852A1
公开(公告)日：2016-03-10
申请号：US14945091
申请日：2015-11-18
Applicant: Monsanto Technology LLC
Inventor： Edwards Allen , Larry A. Gilbertson , Sergey I. Ivashuta , James K. Roberts
IPC: C12N15/82
CPC classification number: C12N15/8218 , C12N15/8216 , C12N15/8274 , C12N15/8285 , C12N15/8286
Abstract: This invention provides a method to autoregulate expression of a transgene susceptible to sRNA silencing by concomitantly transcribing RNA from DNA of a transgene and RNA from DNA from at least one sRNA silencing pathway gene. An aspect of the invention provides use of a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator. Also disclosed are transgenic cells and organisms having in their genome a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator.
Abstract translation: 本发明提供了一种通过从来自至少一个sRNA沉默途径基因的DNA的转基因和RNA的DNA同时转录RNA来自动调节对sRNA沉默敏感的转基因的表达的方法。 本发明的一个方面提供了包含sRNA沉默调节子的转基因DNA和DNA的重组DNA构建体的用途。 还公开了在其基因组中具有重组DNA构建体的转基因细胞和生物体，其包含转基因的DNA和sRNA沉默调节子的DNA。
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公开(公告)号：US08895808B2
公开(公告)日：2014-11-25
申请号：US13715063
申请日：2012-12-14
Applicant: Monsanto Technology LLC
Inventor： Larry A. Gilbertson , Susan J. Johnson
CPC classification number: C12N15/8216 , C12N15/8205 , C12N15/8209 , C12N15/821 , C12N15/8218 , C12N15/8241
Abstract: Methods of, and compositions for, assembling one or more transcription units in a genome without a linked selectable marker or other unwanted transcription unit are provided. Also provided methods of, and compositions for, assembling one or more transcription units in a genome with a reduced frequency of vector backbone.
Abstract translation: 提供了组装一个或多个基因组中没有连接的选择性标记或其他不需要的转录单位的转录单位的方法和组合物。 还提供了组装一个或多个基因组中具有降低的载体骨架频率的转录单位的方法和组合物。
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公开(公告)号：US20240124884A1
公开(公告)日：2024-04-18
申请号：US18538893
申请日：2023-12-13
Applicant: Monsanto Technology LLC
Inventor： Stanton B. Dotson , Larry A. Gilbertson , Jonathan C. Lamb , Brenda Lowe , Zoe P. McCuddin
IPC: C12N15/82
CPC classification number: C12N15/8213 , C12N15/8241 , C12N15/8243 , C12N15/8245 , C12N15/8247 , C12N15/8251 , C12N15/8257 , C12N15/8261 , C12N15/827 , C12N15/8271 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12N15/8289 , C12N15/829
Abstract: The invention provides novel uses of sequence-specific or sequence-directed endonucleases for molecular plant breeding. The invention also provides novel plant transformation vectors and expression cassettes, which include novel combinations of an endonuclease with plant expression and transformation elements. Plants and derivatives thereof produced by such methods are also provided.
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公开(公告)号：US20240117374A1
公开(公告)日：2024-04-11
申请号：US18497825
申请日：2023-10-30
Applicant: Monsanto Technology LLC
Inventor： Timothy K. Ball , Artem G. Evdokimov , Larry A. Gilbertson , Victor M. Guzov , Jeffrey A. Haas , Qing Huai , Sergey Ivashuta , Melissa M. Kemp , Yifei Kong , Thomas M. Malvar , Byron V. Olsen , Parthasarathy Ramaseshadri , Brian E. Weiner
IPC: C12N15/82 , A01N37/44 , A01N37/46 , C07K14/325
CPC classification number: C12N15/8286 , A01N37/44 , A01N37/46 , C07K14/325 , Y02A40/146
Abstract: Pesticidal proteins are provided that combine two or more modes of action into a single molecule. These pesticidal proteins therefor exhibit greater efficacy and/or durability of resistance, for the purposes of pest control, and can be utilized for pest control by provision in the diet of a pest organism, or by topical application to crop plants and/or pests. Methods and compositions for producing and using such proteins are also provided.
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公开(公告)号：US20220267793A1
公开(公告)日：2022-08-25
申请号：US17689613
申请日：2022-03-08
Applicant: Monsanto Technology LLC
Inventor： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. Larosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
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