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    LIQUID BRIDGE AND SYSTEM
    21.
    发明申请
    LIQUID BRIDGE AND SYSTEM 有权
    液桥和系统

    公开(公告)号：US20130112300A1

    公开(公告)日：2013-05-09

    申请号：US13627593

    申请日：2012-09-26

    Applicant: Stokes BIO Limited

    Inventor： Mark Davies ,  Tara Dalton ,  Kiernan Curran

    IPC: F17D1/08

    CPC classification number: B01L3/502784 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01F2215/0037 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502746 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/0838 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/082 ,  C12Q1/686 ,  F17D1/08 ,  Y10T137/87676 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

    Abstract: A bridge comprises a first inlet port, a second inlet port, an outlet port, and a chamber for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber. The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber. The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port, and the droplets combine at the liquid bridge. In another embodiment, a droplet segments into smaller droplets which bridge the gap between the inlet and outlet ports.

    Abstract translation: 桥梁包括第一入口端口，第二入口端口，出口端口和用于硅油的室。 油与反应器液滴密度匹配，使得在室内产生中性浮力的环境。 室内的油通过分离反应器液滴的油不断补充。 这使得液滴在进入室时呈现稳定的毛细管悬浮球形。 球形增长直到足够大以跨越端口之间的间隙，形成轴对称液桥。 从第二入口引入第二液滴导致不稳定的索道桥的形成，其从较细的第二入口快速破裂，并且液滴在液桥处结合。 在另一个实施例中，液滴分段成较小的液滴，桥接入口和出口之间的间隙。

    METHODS FOR CULTURING AND ANALYZING CELLS
    24.
    发明申请
    METHODS FOR CULTURING AND ANALYZING CELLS 审中-公开

    公开(公告)号：US20190105617A1

    公开(公告)日：2019-04-11

    申请号：US16155411

    申请日：2018-10-09

    Applicant: STOKES BIO LIMITED

    Inventor： Mark Davies ,  Tara Dalton

    IPC: B01F5/00 ,  B01F13/00 ,  B01L3/00 ,  B01L7/00 ,  G01N35/08 ,  G01N33/50

    Abstract: A system for analyzing a biological sample may include at least one sample acquisition stage comprising a sample acquisition device for acquiring the biological sample from a sample source; a droplet generator device for forming a droplet wrapped in an immiscible carrier fluid, wherein the wrapped droplet comprises at least the biological sample and a reagent, the droplet generator configured to receive the biological sample transferred from the sample acquisition device; a collection vessel for collecting the wrapped sample droplet from the droplet generator, the vessel configured to contain a carrier fluid for receiving and protecting the sample droplet; and an analysis system for analyzing the wrapped sample droplet and detecting products of a polymerase chain reaction.

    Novel Gene Normalization Methods
    27.
    发明申请
    Novel Gene Normalization Methods 审中-公开
    新型基因归一化方法

    公开(公告)号：US20160083779A1

    公开(公告)日：2016-03-24

    申请号：US14836476

    申请日：2015-08-26

    Applicant: STOKES BIO LIMITED

    Inventor： Mark Davies ,  Tara Dalton

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6809 ,  C12Q1/6886 ,  C12Q2545/101

    Abstract: Measurement of gene expression relative to an endogenous control gene is prone to excessive variability between samples and even replicates. The disclosure provides methods for normalizing expression levels of a gene by scaling gene expression levels to that of the most highly expressed gene in the set of genes whose expression levels are measured, rather than a house-keeping gene.

    Abstract translation: 相对于内源性对照基因的基因表达的测量在样品之间容易发生过度的变异，甚至复制。 本公开提供了通过将基因表达水平与表达水平测定的基因组中最高表达基因的基因表达水平相比较而不是保存基因来使基因表达水平正常化的方法。

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