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    Microfluidic analysis system
    3.
    发明授权
    Microfluidic analysis system 有权

    公开(公告)号：US10676786B2

    公开(公告)日：2020-06-09

    申请号：US15278894

    申请日：2016-09-28

    Applicant: STOKES BIO LIMITED

    Inventor： Mark Davies ,  Tara Dalton

    IPC: C12Q1/686 ,  B01L3/00 ,  B01L7/00 ,  G01N35/08 ,  B01F5/00 ,  B01F13/00 ,  G01N35/10 ,  G01N35/00

    Abstract: A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.

    Microfluidic droplet queuing network
    4.
    发明授权
    Microfluidic droplet queuing network 有权
    微流控液滴排队网络

    公开(公告)号：US09108177B2

    公开(公告)日：2015-08-18

    申请号：US14057301

    申请日：2013-10-18

    Applicant: STOKES BIO LIMITED

    Inventor： Mark Davies ,  Tara Dalton ,  Julie Garvey ,  Kieran Curran ,  Damian Curtin

    IPC: C12M1/34 ,  B01J19/00 ,  B01F13/00 ,  B01F15/02 ,  B01L3/00

    CPC classification number: B01J19/0046 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01J2219/00313 ,  B01J2219/00353 ,  B01J2219/00479 ,  B01J2219/00587 ,  B01J2219/00608 ,  B01L3/5025 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2400/0487 ,  Y10T436/25

    Abstract: A multi-port liquid bridge (1) adds aqueous phase droplets (10) in an enveloping oil phase carrier liquid (11) to a draft channel (4, 6). A chamber (3) links four ports, and it is permanently full of oil (11) when in use. Oil phase is fed in a draft flow from an inlet port (4) and exits through a draft exit port (6) and a compensating flow port (7). The oil carrier and the sample droplets (3) (“aqueous phase”) flow through the inlet port (5) with an equivalent fluid flow subtracted through the compensating port (7). The ports of the bridge (1) are formed by the ends of capillaries held in position in plastics housings. The phases are density matched to create an environment where gravitational forces are negligible. This results in droplets (10) adopting spherical forms when suspended from capillary tube tips. Furthermore, the equality of mass flow is equal to the equality of volume flow. The phase of the inlet flow (from the droplet inlet port (5) and the draft inlet port (4) is used to determine the outlet port (6) flow phase.

    Abstract translation: 多端口液体桥（1）将包含油相载体液体（11）中的水相液滴（10）加入到通风道（4,6）。 室（3）连接四个端口，在使用时永久充满油（11）。 油相在进气口（4）的进料流中进料，并通过排气口（6）和补偿流通口（7）排出。 油载体和样品液滴（“水相”）以相当的流体流通过入口端口（5）流过补偿口（7）。 桥梁（1）的端口由保持在塑料壳体中的位置的毛细管的端部形成。 这些阶段是密度匹配的，以创造引力几乎可忽略的环境。 这导致当从毛细管尖端悬浮时，液滴（10）采用球形。 此外，质量流量的相等性等于体积流量的相等。 入口流（从液滴入口端口5和进气口4）的相位用于确定出口（6）的流动相位。

    Microfluidic analysis system
    6.
    发明授权
    Microfluidic analysis system 有权

    公开(公告)号：US11084039B2

    公开(公告)日：2021-08-10

    申请号：US15429765

    申请日：2017-02-10

    Applicant: STOKES BIO LIMITED

    Inventor： Mark Davies ,  Tara Dalton

    IPC: B01L7/00 ,  B01L3/00 ,  G01N21/64 ,  C12Q1/686

    Abstract: A biological sample analysis system including a sample preparation system forming droplets of segmented sample separated by a carrier fluid immiscible with the sample. The droplets include reaction mixtures for amplification of at least one target nucleic acid. A thermal cycling device having a sample block having a plurality of controlled thermal zones, and a containment structure in thermal communication with the plurality of controlled thermal zones. The containment structure receives and contains the droplets of segmented sample separated by the immiscible carrier fluid from the sample preparation system. A controller for controlling a temperature in each thermal zone of the sample block. A detection system detects electromagnetic radiation emitted from each of the droplets individually from the queue of droplets as they flow past the detection system. A positioning system to facilitate moving a queue of the droplets in the thermal cycling device relative to the detection system.

    Methods of releasing and analyzing cellular components
    7.
    发明授权
    Methods of releasing and analyzing cellular components 有权

    公开(公告)号：US10967338B2

    公开(公告)日：2021-04-06

    申请号：US16155411

    申请日：2018-10-09

    Applicant: STOKES BIO LIMITED

    Inventor： Mark Davies ,  Tara Dalton

    IPC: G01N33/50 ,  B01F5/00 ,  B01F13/00 ,  B01L3/00 ,  B01L7/00 ,  G01N35/08 ,  G01N35/10 ,  G01N35/00

    Abstract: A system for analyzing a biological sample may include at least one sample acquisition stage comprising a sample acquisition device for acquiring the biological sample from a sample source; a droplet generator device for forming a droplet wrapped in an immiscible carrier fluid, wherein the wrapped droplet comprises at least the biological sample and a reagent, the droplet generator configured to receive the biological sample transferred from the sample acquisition device; a collection vessel for collecting the wrapped sample droplet from the droplet generator, the vessel configured to contain a carrier fluid for receiving and protecting the sample droplet; and an analysis system for analyzing the wrapped sample droplet and detecting products of a polymerase chain reaction.

    Liquid bridge and system
    8.
    发明授权
    Liquid bridge and system 有权

    公开(公告)号：US10730051B2

    公开(公告)日：2020-08-04

    申请号：US15131546

    申请日：2016-04-18

    Applicant: STOKES BIO LIMITED

    Inventor： Mark Davies ,  Tara Dalton ,  Kieran Curran

    IPC: B01L3/00 ,  B01F13/00 ,  B01F15/02 ,  C12Q1/68 ,  F17D1/08 ,  C12Q1/686 ,  B01L7/00

    Abstract: A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30). In another embodiment, a droplet (55) segments into smaller droplets which bridge the gap between the inlet and outlet ports.

    MICROFLUIDIC CONNECTOR
    10.
    发明申请
    MICROFLUIDIC CONNECTOR 审中-公开
    微流连接器

    公开(公告)号：US20160123513A1

    公开(公告)日：2016-05-05

    申请号：US14930487

    申请日：2015-11-02

    Applicant: STOKES BIO LIMITED

    Inventor： Mark Davies ,  Tara Dalton

    IPC: F16L41/18

    CPC classification number: F16L41/18 ,  B01L3/5027 ,  B01L3/5635 ,  B01L3/565 ,  B01L2200/027 ,  F16L39/00 ,  Y10T137/0318

    Abstract: A microfluidic connector (1) comprises and enclosure (6, 7), a fluidic inlet port (2) and a fluidic outlet port (3), in the enclosure, in which the inlet and outlet ports (2, 3) are movable with respect to each other, for example, mutual spacing between the inlet and outlet ports (2, 3) is variable. A port (2) is in a fixed part (6) of the enclosure, and another port (3) is in a part (7) of the enclosure which slides with respect to the fixed part. There may be multiple inlet ports (22, 23) and/or multiple outlet ports (24, 25). Also, there may be an auxiliary port (45) for introduction of fluid into the enclosure (47, 48) or removal of fluid from the enclosure.

    Abstract translation: 微流体连接器（1）包括壳体（6,7），流体入口端口（2）和流体出口端口（3），其中入口端口和出口端口（2,3）可移动 彼此相对，例如，入口端口（3）之间的相互间隔是可变的。 端口（2）位于外壳的固定部分（6）中，另一个端口（3）位于外壳的相对于固定部分滑动的部分（7）中。 可以有多个入口端口（22,23）和/或多个出口端口（24,25）。 而且，可以存在用于将流体引入外壳（47,48）或从外壳移除流体的辅助端口（45）。

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