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    Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation
    21.
    发明授权
    Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation 有权

    公开(公告)号：US12158422B2

    公开(公告)日：2024-12-03

    申请号：US18214292

    申请日：2023-06-26

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Eric D. Diebold ,  Bahram Jalali ,  Brandon Buckley

    IPC: G01N21/64 ,  G01N21/05 ,  G02B21/00 ,  G02B21/16 ,  G02B27/10 ,  G02F1/11

    Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

    Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation
    22.
    发明授权
    Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation 有权

    公开(公告)号：US12158421B2

    公开(公告)日：2024-12-03

    申请号：US17745206

    申请日：2022-05-16

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Eric D. Diebold ,  Bahram Jalali ,  Brandon Buckley

    IPC: G01N21/64 ,  G01N21/05 ,  G02B21/00 ,  G02B21/16 ,  G02B27/10 ,  G02F1/11

    Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

    FAST TWO-PHOTON IMAGING BY DIFFRACTED SWEPT-LASER EXCITATION
    24.
    发明申请
    FAST TWO-PHOTON IMAGING BY DIFFRACTED SWEPT-LASER EXCITATION 审中-公开

    公开(公告)号：US20190226989A1

    公开(公告)日：2019-07-25

    申请号：US16233242

    申请日：2018-12-27

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  UNIVERSITAET ZU LUEBECK

    Inventor： Sebastian Karpf ,  Bahram Jalali ,  Robert Huber

    IPC: G01N21/64 ,  H01S3/00 ,  H01S3/067 ,  G02B21/00

    Abstract: An apparatus and methods for high-speed non-linear spectrally encoded multi-photon imaging that are particularly suited for use in two photon fluorescence and fluorescence lifetime imaging. The system is capable of optical image compression and scale invariant digital zoom. A wavelength agile laser with digitally synthesized electro-optic modulation in a master oscillator-power amplifier configuration is combined with spectral encoding to eliminate the speed limitations of inertial scanning. The technique for fast two photon fluorescent imaging with simultaneous lifetime imaging independently detects the location, amplitude and lifetime of fluorescent emission by synthesizing a sequential excitation beam via digital electro-optic modulation of a quasi-CW swept source followed by time encoded detection. For fluorescent imaging, spectral and temporal mappings are employed separately, with quasi-CW spectral encoding used for pumping and time encoding for constructing the image at fluorescence wavelength.

    Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation
    27.
    发明授权
    Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation 有权
    使用射频多路复用荧光成像的装置和方法

    公开(公告)号：US09423353B2

    公开(公告)日：2016-08-23

    申请号：US14792282

    申请日：2015-07-06

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Eric D. Diebold ,  Bahram Jalali ,  Brandon Buckley

    IPC: G01N21/64 ,  G02B21/16

    CPC classification number: G01N21/6408 ,  G01N21/05 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2021/6415 ,  G01N2021/6419 ,  G01N2201/06113 ,  G02B21/0076 ,  G02B21/0084 ,  G02B21/16 ,  G02B27/1006 ,  G02F1/11

    Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

    Abstract translation: 使用射频复用激发进行荧光成像的装置和方法。 一个装置将激发激光束分成Mach-Zehnder干涉仪的两个臂。 第一光束中的光被声光偏转器偏移，声光偏转器由相位设计的射频梳驱动，旨在最小化峰值与平均功率比。 该RF梳产生具有一定范围的输出角和频移的多个偏转光束。 第二个波束使用声光移频器在频率上移动。 在第二分光器组合之后，使用传统的激光扫描显微镜透镜系统将两个光束聚焦到样品上的线上。 声光偏转器对整行像素的同时激励进行频率编码，这使得能够使用单个光电倍增管和数字相位相干信号恢复技术来检测和解复用荧光图像。

    Methods for optical amplified imaging using a two-dimensional spectral brush
    28.
    发明授权
    Methods for optical amplified imaging using a two-dimensional spectral brush 有权
    使用二维光谱刷的光学放大成像方法

    公开(公告)号：US08987649B2

    公开(公告)日：2015-03-24

    申请号：US13861582

    申请日：2013-04-12

    Applicant: The Regents of the University of California

    Inventor： Bahram Jalali ,  Keisuke Goda ,  Kevin Kin-Man Tsia

    IPC: H01L27/00 ,  H01L31/00 ,  G02B21/36

    CPC classification number: G02B21/361 ,  A61B1/00009 ,  A61B1/00193 ,  A61B1/0638 ,  A61B5/0062 ,  A61B5/0066 ,  A61B5/0075 ,  A61B5/0086 ,  A61B5/7257 ,  G01B9/02004 ,  G01B9/02014 ,  G01B9/02028 ,  G01B9/02044 ,  G01B9/02091 ,  G01B11/2441 ,  G01B2290/20 ,  G01B2290/65 ,  G06K9/2036 ,  G06K2209/05 ,  H04N9/045

    Abstract: An apparatus and method for ultrafast real-time optical imaging that can be used for imaging dynamic events such as microfluidics or laser surgery is provided. The apparatus and methods encode spatial information from a sample into a back reflection of a two-dimensional spectral brush that is generated with a two-dimensional disperser and a light source that is mapped in to the time domain with a temporal disperser. The temporal waveform is preferably captured by an optical detector, converted to an electrical signal that is digitized and processed to provide two dimensional and three dimensional images. The produced signals can be optically or electronically amplified. Detection may be improved with correlation matching against a database in the time domain or the spatial domain. Embodiments for endoscopy, microscopy and simultaneous imaging and laser ablation with a single fiber are illustrated.

    Abstract translation: 提供了一种用于超快速实时光学成像的装置和方法，可用于成像诸如微流体或激光手术的动态事件成像。 该装置和方法将来自样本的空间信息编码成二维光谱刷的背反射，二维光谱刷是用二维分散器和光源以及时间分散器映射到时域而生成的。 时间波形优选地由光学检测器捕获，转换成被数字化和处理以提供二维和三维图像的电信号。 产生的信号可以是光学或电子放大的。 可以通过与时域或空间域中的数据库的相关匹配来改进检测。 示出了用于内窥镜检查，显微镜和同时成像以及使用单纤维的激光烧蚀的实施例。

    Differential interference contrast serial time encoded amplified microscopy
    29.
    发明授权
    Differential interference contrast serial time encoded amplified microscopy 有权
    差分干涉对比串行时间编码放大显微镜

    公开(公告)号：US08654441B2

    公开(公告)日：2014-02-18

    申请号：US13668239

    申请日：2012-11-03

    Applicant: The Regents of the University of California

    Inventor： Bahram Jalali ,  Ali Motafakker-Fard

    IPC: G02B21/00

    CPC classification number: G01J1/0411 ,  G02B21/002 ,  G02B21/14 ,  H04N5/14

    Abstract: We describe methods and apparatus for high-speed high-contrast imaging one-, two- and three-dimensional imaging enabled by differential interference contrast time encoded amplified microscopy of transparent media without the need for chemical staining, that are suitable for a broad range of applications from semiconductor process monitoring to blood screening. Our methods and apparatus build on a unique combination of serial time-encoded amplified microscopy (STEAM) and differential interference contrast (DIC) microscopy. These methods and apparatus are ideally suited for identification of rare diseased cells in a large population of healthy cells and have the potential to revolutionize blood analysis and pathology including identification of cancer cells, such as Circulating Tumor Cells (CTC) in early stage disease.

    Abstract translation: 我们描述了用于高速高对比度成像的一维，二维和三维成像的方法和装置，其通过差示干涉对比度时间编码的透明介质的放大显微镜而不需要化学染色，其适用于广泛的 从半导体工艺监测到血液筛查的应用。 我们的方法和设备基于串行时间编码放大显微镜（STEAM）和微分干涉对比（DIC）显微镜的独特组合。 这些方法和装置非常适用于大量健康细胞中罕见病变细胞的鉴定，并具有革命性的血液分析和病理学的潜力，包括癌症细胞的鉴定，如早期疾病中的循环肿瘤细胞（CTC）。

    APPARATUS AND METHOD FOR DISPERSIVE FOURIER-TRANSFORM IMAGING
    30.
    发明申请
    APPARATUS AND METHOD FOR DISPERSIVE FOURIER-TRANSFORM IMAGING 有权
    用于分散式FOURIER-TRANSFORM成像的装置和方法

    公开(公告)号：US20130221102A1

    公开(公告)日：2013-08-29

    申请号：US13770678

    申请日：2013-02-19

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Bahram Jalali ,  Keisuke Goda ,  Kevin Kin-Man Tsia

    IPC: G06K7/14

    CPC classification number: G06K7/1439 ,  G06K7/10712 ,  G06K7/10831 ,  G06K7/14

    Abstract: A barcode reading apparatus and method in which the spectrum of a probe light is first Fourier-transformed into space, directed upon a barcode, and then Fourier-transformed converting the spectrally encoded barcode pattern to a time domain waveform. In one implementation, the Fourier transformation from the spectrum domain into a spatial domain is performed by a dispersive element, while the Fourier transformation from the spectrally encoded barcode pattern to a time domain waveform is performed by group-velocity dispersion (GVD). The temporally encoded barcode pattern is detected by a photodetector, digitized by a digitizer, and analyzed by a digital signal processor. The invention is applicable to a number of fields which involve the reading of one- and two-dimensional barcodes, displacement sensing, surface measurements, measurement of width and gap, flow cytometry, reading of optical media, presense or absence detection, and other related fields.

    Abstract translation: 一种条形码读取装置和方法，其中探针光的光谱首先被傅里叶变换为空间，指向条形码，然后经频谱转换成频谱编码的条形码图案到时域波形。 在一个实现中，通过色散元素执行从频域到空间域的傅立叶变换，而通过组速度色散（GVD）执行从频谱编码的条形码图案到时域波形的傅里叶变换。 时间编码的条形码图案由光电检测器检测，由数字化仪数字化，并由数字信号处理器分析。 本发明可应用于涉及读取一维和二维条形码，位移检测，表面测量，宽度和间隙测量，流式细胞术，光学介质读数，预存或不存在检测等多个领域 领域。

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