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    Recombinant streptavidin-metallothionein chimeric protein having biological recognition specificity
    21.
    发明授权
    Recombinant streptavidin-metallothionein chimeric protein having biological recognition specificity 失效
    具有生物识别特异性的重组链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白

    公开(公告)号：US06391590B1

    公开(公告)日：2002-05-21

    申请号：US07780717

    申请日：1991-10-21

    申请人： Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

    发明人： Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

    IPC分类号： C12N1562

    CPC分类号： C07H21/04 ,  C07K14/36 ,  C07K14/825 ,  C07K2319/00 ,  C07K2319/22

    摘要： Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.

    摘要翻译： 具有生物识别特异性的链霉亲和素 - 金属硫蛋白嵌合蛋白，其中链霉亲和素部分提供高亲和力生物素结合和金属硫蛋白部分提供高亲和力的金属结合。 链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白对生物素和重金属离子的结合亲和力允许特异性掺入，缀合或标记含有生物素与各种重金属离子的任何生物材料。

    METHOD FOR DETECTING AND QUANTIFYING RARE MUTATIONS/POLYMORPHISMS
    22.
    发明申请
    METHOD FOR DETECTING AND QUANTIFYING RARE MUTATIONS/POLYMORPHISMS 有权
    检测和量化稀有突变/多态性的方法

    公开(公告)号：US20110097712A1

    公开(公告)日：2011-04-28

    申请号：US12496390

    申请日：2009-07-01

    申请人： Charles R. Cantor ,  Chunming Ding

    发明人： Charles R. Cantor ,  Chunming Ding

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q2600/156 ,  C12Q2525/186 ,  C12Q2535/125 ,  C12Q2565/627 ,  C12Q2545/101

    摘要： The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s).

    摘要翻译： 本发明涉及一种用于检测和定量核酸样品中罕见突变的方法。 正在研究的核酸分子可以是DNA或RNA。 罕见突变可以是任何类型的功能或非功能性核酸变化或突变，例如缺失，插入，易位，反转，一个或多个碱基取代或多态性。 因此，当目标是罕见的已知的与野生型或更常见的核酸变体混合的核酸变体时，本发明的方法可用于检测例如诊断，预后和随访应用中的罕见突变 （s）。

    METHOD FOR DE NOVO DETECTION OF SEQUENCES IN NUCLEIC ACIDS: TARGET SEQUENCING BY FRAGMENTATION
    23.
    发明申请
    METHOD FOR DE NOVO DETECTION OF SEQUENCES IN NUCLEIC ACIDS: TARGET SEQUENCING BY FRAGMENTATION 有权
    在核酸中检测序列的方法：通过分段进行的目标序列

    公开(公告)号：US20090075288A1

    公开(公告)日：2009-03-19

    申请号：US12259376

    申请日：2008-10-28

    申请人： Charles R. Cantor ,  Fouad A. Siddiqi

    发明人： Charles R. Cantor ,  Fouad A. Siddiqi

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6872 ,  C12Q2525/101 ,  C12Q2523/107

    摘要： The present invention provides a method for determining nucleic acid sequences of a template nucleic acid that requires no prior knowledge of the nucleic acid sequence present in the template nucleic acid. The method is based on combining information about the mass of a fragment, the mass of any one nucleotide and the combinations thereof, and the sequence specificity of a nucleotide cutter, either enzymatic or chemical cutter, to determine a sequence of a nucleic acid fragment. This method allows for de novo detection of sequences in a target nucleic acid without requiring any prior sequence information. This method is called Partial Sequencing by Fragmentation (PSBF) and it works by fragmenting a target into oligo- or polynucleotides whose masses or lengths are uniquely associated with known sequences. The identities of these sequences are determined solely by the specific fragmentation method used, and are always independent of the target. PSBF can be implemented using electrophoresis, mass spectrometry or any other method that can be used to distinguish the size of the cut nucleic acid sequence fragments.

    摘要翻译： 本发明提供了一种确定模板核酸的核酸序列的方法，其不需要模板核酸中存在的核酸序列的先验知识。 该方法基于组合关于片段的质量，任何一个核苷酸的质量及其组合的信息以及核苷酸切割剂（酶或化学切割机）的序列特异性，以确定核酸片段的序列。 该方法允许从头检测靶核酸中的序列，而不需要任何先前的序列信息。 这种方法被称为通过片段化分段测序（PSBF），它的作用是通过将目标片段分成其质量或长度与已知序列唯一相关的寡核苷酸或多核苷酸。 这些序列的身份完全由所使用的具体碎片方法确定，并且始终与目标无关。 PSBF可以使用电泳，质谱法或可用于区分切割的核酸序列片段的大小的任何其它方法来实现。

    Method for De Novo Detection of Sequences in Nucleic Acids: Target Sequencing by Fragmentation
    24.
    发明申请
    Method for De Novo Detection of Sequences in Nucleic Acids: Target Sequencing by Fragmentation 失效
    核酸序列的新检测方法：通过分段进行靶序列测定

    公开(公告)号：US20080096194A1

    公开(公告)日：2008-04-24

    申请号：US11547765

    申请日：2005-04-08

    申请人： Charles R. Cantor ,  Fouad A. Siddiqi

    发明人： Charles R. Cantor ,  Fouad A. Siddiqi

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6872 ,  C12Q2525/101 ,  C12Q2523/107

    摘要： The present invention provides a method for determining nucleic acid sequences of a template nucleic acid that requires no prior knowledge of the nucleic acid sequence present in the template nucleic acid. The method is based on combining information about the mass of a fragment, the mass of any one nucleotide and the combinations thereof, and the sequence specificity of a nucleotide cutter, either enzymatic or chemical cutter, to determine a sequence of a nucleic acid fragment. This method allows for de novo detection of sequences in a target nucleic acid without requiring any prior sequence information. This method is called Partial Sequencing by Fragmentation (PSBF) and it works by fragmenting a target into oligo- or polynucleotides whose masses or lengths are uniquely associated with known sequences. The identities of these sequences are determined solely by the specific fragmentation method used, and are always independent of the target. PSBF can be implemented using electrophoresis, mass spectrometry or any other method that can be used to distinguish the size of the cut nucleic acid sequence fragments.

    摘要翻译： 本发明提供了一种确定模板核酸的核酸序列的方法，其不需要模板核酸中存在的核酸序列的先验知识。 该方法基于组合关于片段的质量，任何一个核苷酸的质量及其组合的信息以及核苷酸切割剂（酶或化学切割机）的序列特异性，以确定核酸片段的序列。 该方法允许从头检测靶核酸中的序列，而不需要任何先前的序列信息。 这种方法被称为通过片段化分段测序（PSBF），它的作用是通过将目标片段分成其质量或长度与已知序列唯一相关的寡核苷酸或多核苷酸。 这些序列的身份完全由所使用的具体碎片方法确定，并且始终与目标无关。 PSBF可以使用电泳，质谱法或可用于区分切割的核酸序列片段的大小的任何其它方法来实现。

    Design and synthesis of bispecific reagents: use of double stranded DNAs as chemically and spatially defined cross-linkers
    27.
    发明授权
    Design and synthesis of bispecific reagents: use of double stranded DNAs as chemically and spatially defined cross-linkers 失效
    双特异性试剂的设计和合成：使用双链DNA作为化学和空间定义的交联剂

    公开(公告)号：US5849878A

    公开(公告)日：1998-12-15

    申请号：US481065

    申请日：1995-06-07

    申请人： Charles R. Cantor ,  Roy S. Chuck ,  Doris B. Tse

    发明人： Charles R. Cantor ,  Roy S. Chuck ,  Doris B. Tse

    IPC分类号： C07K16/28 ,  C07K16/46 ,  C07K17/00 ,  C07K19/00

    CPC分类号： C07K16/2812 ,  C07K16/2809 ,  C07K16/468

    摘要： The invention relates to bis-protein-DNA conjugates. A protein having a specific ligand binding activity is covalently linked to each end of a derivatized DNA molecule. These bis-protein-DNA conjugates can be used for immunoassays, PCR assays and measuring distances between proteins at up to 3.4 A resolution. The invention also relates to methods of synthesizing these bis-protein-DNA conjugates. Synthesis of the conjugates entails derivatizing the 5' or 3' end of a DNA oligonucleotide and covalently linking that DNA to a protein. The DNA can be conjugated to the proteins, including antibodies or Fab' fragments, using disulfide bond linkage.

    摘要翻译： 本发明涉及双蛋白-DNA缀合物。 具有特异性配体结合活性的蛋白质与衍生的DNA分子的每个末端共价连接。 这些双蛋白-DNA缀合物可用于免疫测定，PCR测定和测量蛋白质之间的距离，高达3.4A分辨率。 本发明还涉及合成这些双蛋白-DNA缀合物的方法。 共轭物的合成需要衍生DNA寡核苷酸的5'或3'末端并共价连接该DNA与蛋白质。 使用二硫键可将DNA与蛋白质缀合，包括抗体或Fab'片段。

    Nucleic acid quantification products and processes
    29.
    发明授权
    Nucleic acid quantification products and processes 有权

    公开(公告)号：US10533215B2

    公开(公告)日：2020-01-14

    申请号：US13129797

    申请日：2009-11-20

    申请人： Charles R. Cantor

    发明人： Charles R. Cantor

    IPC分类号： C12Q1/68 ,  C12Q1/6837

    摘要： Described herein are products and processes for nucleic acid quantification, which are in part useful for detecting and determining the nucleotide sequence of rare nucleic acids (i.e., low copy number nucleic acids) in a sample. Such products and processes are useful for reducing the dynamic range among different nucleic acid species.

    Methods and compositions for the analysis of nucleic acids
    30.
    发明授权
    Methods and compositions for the analysis of nucleic acids 有权
    用于分析核酸的方法和组合物

    公开(公告)号：US08852864B2

    公开(公告)日：2014-10-07

    申请号：US12863169

    申请日：2009-01-16

    申请人： Charles R. Cantor

    发明人： Charles R. Cantor

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2525/161 ,  C12Q2563/185

    摘要： Provided herein are compositions and methods for analysis of nucleic acids, including, methods and compositions for genotyping, haplotyping, sequencing and performing other genetic and epigenetic analysis on nucleic acids, for example. In some embodiments, methods and compositions suitable for whole-genome sequencing on single molecules of nucleic acid are provided. In some embodiments, analysis of single molecules of nucleic acid are performed in conjunction with nanopores and/or nanopore devices.

    摘要翻译： 本文提供了用于核酸分析的组合物和方法，包括用于基因分型，单倍型，测序和对核酸进行其他遗传和表观遗传学分析的方法和组合物。 在一些实施方案中，提供适用于单分子核酸上的全基因组测序的方法和组合物。 在一些实施方案中，结合纳米孔和/或纳米孔装置进行单分子核酸的分析。