公开(公告)号：US10527626B2

公开(公告)日：2020-01-07

申请号：US14903012

申请日：2014-07-01

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Mengxia Zhao ,  Wyatt Nelson ,  Perry G. Schiro

IPC: G01N33/536 ,  G01N33/574 ,  B01L3/00 ,  G01N33/53

Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.

公开(公告)号：US10150841B2

公开(公告)日：2018-12-11

申请号：US14366863

申请日：2012-12-27

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Yu Rong ,  Yong Zhang ,  Yi-Che Wu ,  Yang-Hsiang Chan ,  Xuanjun Zhang ,  Jiangbo Yu ,  Wei Sun

IPC: C08G75/32 ,  C08G79/00 ,  G01N33/58 ,  H01L51/00 ,  A61K49/00 ,  C09B69/10 ,  G01N21/64 ,  H01L51/50 ,  B82Y40/00 ,  B82Y30/00

Abstract: Polymers, monomers, chromophoric polymer dots and related methods are provided. Highly fluorescent chromophoric polymer dots with narrow-band emissions are provided. Methods for synthesizing the chromophoric polymers, preparation methods for forming the chromophoric polymer dots, and biological applications using the unique properties of narrow-band emissions are also provided.

公开(公告)号：US20160096172A1

公开(公告)日：2016-04-07

申请号：US14869871

申请日：2015-09-29

Applicant: University of Washington

Inventor： Daniel T. Chiu ,  Dawn E. Cohen ,  Gavin D.M. Jeffries

IPC: B01L3/00

Abstract: Embodiments of the present invention relate to methods and apparatuses for the discretization and manipulation of sample volumes that is simple, robust, and versatile. It is a fluidic device that partitions a sample by exploiting the interplay between fluidic forces, interfacial tension, channel geometry, and the final stability of the formed droplet and/or discretized volume. These compartmentalized volumes allow for isolation of samples and partitioning into a localized array that can subsequently be manipulated and analyzed. The isolation of the discretized volumes along with the device's inherent portability render our invention versatile for use in many areas, including but not limited to PCR, digital PCR, biological assays for diagnostics and prognostics, cancer diagnosis and prognosis, high throughput screening, single molecule and single cell reactions or assays, the study crystallization and other statistical processes, protein crystallization, drug screening, environmental testing, and the coupling to a wide range of analytical detection techniques for biomedical assays and measurements. The minimal fluid interconnects and simple flow geometry makes the device easy to use and implement, economical to fabricate and operate, and robust in its operations.

Abstract translation: 本发明的实施例涉及用于简单，稳健和多用途的样品体积的离散化和操作的方法和装置。 它是通过利用流体力，界面张力，通道几何形状和形成的液滴和/或离散体积的最终稳定性之间的相互作用分隔样品的流体装置。 这些分隔的容积允许将样品和分离分离成可随后被操纵和分析的局部阵列。 离散体积的分离以及设备的固有可移植性使我们的发明具有多功能性，可用于许多领域，包括但不限于PCR，数字PCR，用于诊断和预后的生物测定，癌症诊断和预后，高通量筛选，单分子 和单细胞反应或测定，研究结晶和其他统计过程，蛋白质结晶，药物筛选，环境测试以及与广泛的用于生物医学测定和测量的分析检测技术的耦合。 最小的流体互连和简单的流动几何形状使得设备易于使用和实施，经济地制造和操作，并且在其操作中稳固。

公开(公告)号：US20140350183A1

公开(公告)日：2014-11-27

申请号：US14366863

申请日：2012-12-27

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Yu Rong ,  Yong Zhang ,  Yi-Che Wu ,  Yang-Hsiang Chan ,  Xuanjun Zhang ,  Jiangbo Yu ,  Wei Sun

IPC: C08G75/32 ,  H01L51/00 ,  G01N33/58 ,  C08G79/00

CPC classification number: C08G75/32 ,  A61K49/0019 ,  A61K49/0067 ,  B82Y30/00 ,  B82Y40/00 ,  C08G79/00 ,  C09B69/105 ,  G01N33/582 ,  G01N33/587 ,  G01N2021/6439 ,  H01L51/0034 ,  H01L51/0036 ,  H01L51/0039 ,  H01L51/0043 ,  H01L51/5012

Abstract: Polymers, monomers, chromophoric polymer dots and related methods are provided. Highly fluorescent chromophoric polymer dots with narrow-band emissions are provided. Methods for synthesizing the chromophoric polymers, preparation methods for forming the chromophoric polymer dots, and biological applications using the unique properties of narrow-band emissions are also provided.

Abstract translation: 提供聚合物，单体，发色聚合物点和相关方法。 提供了具有窄带辐射的高度荧光发色聚合物点。 还提供了合成发色聚合物的方法，形成发色聚合物点的制备方法和使用窄带发射的独特性质的生物应用。

公开(公告)号：US12270067B2

公开(公告)日：2025-04-08

申请号：US18058530

申请日：2022-11-23

Applicant: University of Washington ,  LAMPROGEN, INC.

Inventor： Daniel T. Chiu ,  Jiangbo Yu ,  Changfeng Wu ,  Kai Sun

IPC: G01N21/64 ,  B82Y15/00 ,  B82Y30/00 ,  C09K11/07 ,  C12Q1/00

Abstract: The present disclosure provides nanoparticle transducers and methods of use thereof for the detection of analyte concentrations in a fluid. Nanoparticle transducers can comprise a nanoparticle, such as a Pdot, coupled to an enzyme that catalyzes a reaction with the analyte. The nanoparticle transducers further comprise chromophores that emit fluorescence that varies as a function of the concentration of one of the elements of the reaction. The nanoparticle transducer thus changes fluorescence as the analyte concentration changes, transforming analyte concentration values into fluorescence intensities. The measurement of these intensities provides a measurement of the analyte concentration. The nanoparticle transducers are biocompatible, allowing for use in vivo, for the monitoring of analyte blood concentrations such as blood glucose concentrations.

公开(公告)号：US20230159979A1

公开(公告)日：2023-05-25

申请号：US17904916

申请日：2021-04-15

Applicant: UNIVERSITY OF WASHINGTON ,  LAMPROGEN, INC.

Inventor： Daniel T. Chiu ,  Haobin Chen ,  Jiangbo Yu

IPC: C12Q1/32 ,  C12Q1/00 ,  C09K11/06 ,  C09K11/02

CPC classification number: C12Q1/32 ,  C12Q1/008 ,  C09K11/06 ,  C09K11/025 ,  B82Y20/00

Abstract: Transducers, kits, systems, and methods for determining a concentration of an analyte are described. In an embodiment, the transducers include a chromophore; and an enzyme physically associated with the chromophore. In an embodiment, the transducer is configured to catalyze a reaction comprising a plurality of reaction elements. In an embodiment, the plurality of reaction elements comprises one or more reactants including the analyte and one or more products. In an embodiment, an amount of fluorescence emitted from the chromophore is determined by a concentration of a reaction element of the plurality of reaction elements.

公开(公告)号：US11648560B2

公开(公告)日：2023-05-16

申请号：US16639083

申请日：2018-08-15

Applicant: UNIVERSITY OF WASHINGTON ,  MICAREO INC.

Inventor： Daniel T. Chiu ,  Mengxia Zhao ,  Eleanor S. Shah ,  Perry G. Schiro ,  Hui Min Yu ,  Wei-Feng Fang ,  Jui-Lin Chen

IPC: B01L3/00 ,  G01N15/14 ,  G01N33/49 ,  G01N15/10

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/502738 ,  G01N15/1434 ,  G01N15/1459 ,  G01N33/4915 ,  B01L2200/0663 ,  B01L2300/06 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2400/06 ,  B01L2400/084 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2015/1486

Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and can be contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.

公开(公告)号：US11480575B2

公开(公告)日：2022-10-25

申请号：US16702176

申请日：2019-12-03

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. Chiu ,  Mengxia Zhao ,  Wyatt Nelson ,  Perry G. Schiro

IPC: G01N33/574 ,  B01L3/00 ,  G01N15/06 ,  G01N15/14 ,  G01N33/49 ,  G01N21/64 ,  G01N33/53 ,  G01N35/10 ,  G01N35/00 ,  G01N15/10

Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.

公开(公告)号：US20220252607A1

公开(公告)日：2022-08-11

申请号：US17672586

申请日：2022-02-15

Applicant: University of Washington ,  Clemson University Research Foundation

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Jason McNeill ,  Jiangbo Yu

IPC: G01N33/58 ,  C09K11/06

Abstract: The present invention provides, among other aspects, functionalized chromophoric polymer dots comprising a hydrophobic core and a hydrophilic cap, and bioconjugates thereof. Also provided are improved methods for preparing functionalized chromophoric polymer dots. Methods for in vivo imaging and molecular labeling are also disclosed.

公开(公告)号：US11408884B2

公开(公告)日：2022-08-09

申请号：US16702228

申请日：2019-12-03

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. Chiu ,  Wei Sun ,  Jiangbo Yu ,  Changfeng Wu ,  Fangmao Ye

IPC: G01N33/58 ,  C09K11/06 ,  G01N33/543 ,  C09K11/02 ,  C08G61/02 ,  C08G73/00 ,  C08G75/32 ,  G01N33/533

Abstract: Lyophilized chromophoric polymer dot compositions are provided. Also disclosed are methods of making and using the lyophilized compositions, methods of dispersing the lyophilized compositions in aqueous solutions and kits supplying the compositions.