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    E.coli mutant containing mutant genes related with tryptophan biosynthesis and production method of tryptophan by using the same
    21.
    发明授权
    E.coli mutant containing mutant genes related with tryptophan biosynthesis and production method of tryptophan by using the same 有权
    含有与色氨酸生物合成相关的突变基因的大肠杆菌突变体和使用其的色氨酸的生产方法

    公开(公告)号：US07638312B2

    公开(公告)日：2009-12-29

    申请号：US10582196

    申请日：2004-11-23

    申请人： Young-Hoon Park ,  Sang-jo Lim ,  Byoung-Hoon Kim ,  Seong-Jun Kim ,  Ho-Soo Lim

    发明人： Young-Hoon Park ,  Sang-jo Lim ,  Byoung-Hoon Kim ,  Seong-Jun Kim ,  Ho-Soo Lim

    IPC分类号： C12P13/22 ,  C12N9/00 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C07K14/245 ,  C12N9/88 ,  C12P13/227

    摘要： The present invention relates to a Tryptophan-producing E. coli mutant strain CJ285 (KCCM-10534) containing single or multi mutant genes related with Tryptophan biosynthesis and production method of Tryptophan using the same. More particularly, DNA base sequences and amino acid sequences aroF, aroG, trpR, and tyrR originated from tryptophan producing E. coli mutant strain CJ285 (KCCM-10534) and related with Tryptophan biosynthesis, are disclosed, and E. coli CJ285 containing at least one of the mutant genes is cultivated directly in a glucose-containing fermentation medium, whereby L-tryptophan can be accumulated in the culture medium.

    摘要翻译： 本发明涉及含有与色氨酸生物合成相关的单个或多个突变基因的色氨酸产生大肠杆菌突变菌株CJ285（KCCM-10534）和使用其的色氨酸生产方法。 更具体地，公开了源自产生色氨酸的大肠杆菌突变菌株CJ285（KCCM-10534）并与色氨酸生物合成相关的DNA碱基序列和氨基酸序列aroF，aroG，trpR和tyrR，并且至少含有至少含有 其中一个突变基因直接在含葡萄糖的发酵培养基中培养，从而可以将L-色氨酸堆积在培养基中。

    Alleles of the GND gene from coryneform bacteria
    23.
    发明授权
    Alleles of the GND gene from coryneform bacteria 失效
    来自棒状细菌的GND基因的等位基因

    公开(公告)号：US07563602B2

    公开(公告)日：2009-07-21

    申请号：US12016728

    申请日：2008-01-18

    申请人： Georg Thierbach ,  Brigitte Bathe ,  Natalie Schischka

    发明人： Georg Thierbach ,  Brigitte Bathe ,  Natalie Schischka

    IPC分类号： C12P13/22 ,  C12P13/12 ,  C12P13/10 ,  C12N1/21

    CPC分类号： C12P13/22 ,  C12N9/0006 ,  C12P13/08 ,  C12P13/227

    摘要： The invention relates to mutants and alleles of the gnd gene from coryneform bacteria coding for 6-phosphogluconate dehydrogenases which contain at position 329 or a comparable position of the amino acid sequence any amino acid other than L-valine, and to processes for the production of amino acids, preferably L-lysine and L-tryptophan, by fermentation using bacteria that contain these alleles.

    摘要翻译： 本发明涉及编码6-磷酸葡萄糖酸脱氢酶的棒状细菌的gnd基因的突变体和等位基因，其含有第329位或氨基酸序列与L-缬氨酸以外的任何氨基酸的可比较位置，以及用于生产 氨基酸，优选L-赖氨酸和L-色氨酸，通过使用含有这些等位基因的细菌进行发酵。

    Method for the Fermentative Production of L-Amino Acids With the Aid of Coryneform Bacteria Capable of Using Glycerin as the Only Carbon Source
    24.
    发明申请
    Method for the Fermentative Production of L-Amino Acids With the Aid of Coryneform Bacteria Capable of Using Glycerin as the Only Carbon Source 有权
    使用能够使用甘油作为唯一碳源的棒状杆菌细菌的发酵L-氨基酸的发酵方法

    公开(公告)号：US20080293100A1

    公开(公告)日：2008-11-27

    申请号：US11816539

    申请日：2006-09-27

    申请人： Volker F. Wendisch ,  Doris Rittmann ,  Hermann Sahm ,  Caroline Kreutzer

    发明人： Volker F. Wendisch ,  Doris Rittmann ,  Hermann Sahm ,  Caroline Kreutzer

    IPC分类号： C12P13/08 ,  C12N1/21 ,  C12P13/22

    CPC分类号： C12N1/20 ,  C12P13/04 ,  C12P13/08 ,  C12P13/227

    摘要： The invention relates to a method for producing L-amino acids in which the following steps are carried out: a) the recombinant coryneform bacteria which produce the desired L-amino acid and in which at least one or several of the heterologous polynucleotides of the glycerol metabolism, selected among the group comprising glpA, glpB, glpC, glpD, glpE, glpF, glpG, glpK, glpQ, glpT, glpX, gldA, dhaK, dhaL, dhaM, dhaR, fsa, and talC, is/are expressed are cultivated in a medium containing glycerol or one or several other optional C sources in conditions in which the desired L-amino acid is enriched in the medium or the cells; and, optionally, b) the desired L-amino acid is isolated, all or fractions (>0 to 100 percent) of the components of the fermentation broth and/or biomass optionally remaining in the final product. In said method, bacteria are used in which other genes of the biosynthesis pathway of the desired L-amino acid are additionally reinforced or in which the metabolism pathways reducing the formation of the desired L-amino acid are eliminated at least in part.

    摘要翻译： 本发明涉及一种生产L-氨基酸的方法，其中进行以下步骤：a）产生所需L-氨基酸的重组棒状细菌，其中至少一种或几种甘油的异源多核苷酸 被表达为包括glpA，glpB，glpC，glpD，glpE，glpF，glpG，glpK，glpQ，glpG，glpX，gldA，dhaK，dhaL，dhaM，dhaR，fsa和talC的组中的代谢 在含有所需L-氨基酸在培养基或细胞中富集的条件下，在含有甘油或一种或几种其它任选的C源的培养基中; 和任选地，b）分离所需的L-氨基酸，所有或发酵液和/或生物质组分的组分（> 0至100％）任选保留在最终产物中。 在所述方法中，使用细菌，其中所需L-氨基酸的生物合成途径的其它基因被额外增强，或者至少部分地消除了减少所需L-氨基酸形成的代谢途径。

    Alleles of the gnd gene from coryneform bacteria
    29.
    发明申请
    Alleles of the gnd gene from coryneform bacteria 有权
    来自棒状细菌的gnd基因的等位基因

    公开(公告)号：US20060246554A1

    公开(公告)日：2006-11-02

    申请号：US11227138

    申请日：2005-09-16

    申请人： Georg Thierbach ,  Brigitte Bathe ,  Natalie Schischka

    发明人： Georg Thierbach ,  Brigitte Bathe ,  Natalie Schischka

    IPC分类号： C12P13/22 ,  C12P13/12 ,  C12P13/10 ,  C12N1/21

    CPC分类号： C12P13/22 ,  C12N9/0006 ,  C12P13/08 ,  C12P13/227

    摘要： The invention relates to mutants and alleles of the gnd gene from coryneform bacteria coding for 6-phosphogluconate dehydrogenases which contain at position 329 or a comparable position of the amino acid sequence any amino acid other than L-valine, and to processes for the production of amino acids, preferably L-lysine and L-tryptophan, by fermentation using bacteria that contain these alleles.

    摘要翻译： 本发明涉及编码6-磷酸葡萄糖酸脱氢酶的棒状细菌的gnd基因的突变体和等位基因，其含有第329位或氨基酸序列与L-缬氨酸以外的任何氨基酸的可比较位置，以及用于生产 氨基酸，优选L-赖氨酸和L-色氨酸，通过使用含有这些等位基因的细菌进行发酵。