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    Methods and organisms for the growth-coupled production of succinate
    301.
    发明申请
    Methods and organisms for the growth-coupled production of succinate 审中-公开

    公开(公告)号：US20070111294A1

    公开(公告)日：2007-05-17

    申请号：US11518502

    申请日：2006-09-08

    Applicant: Anthony Burgard ,  Stephen Van Dien

    Inventor： Anthony Burgard ,  Stephen Van Dien

    IPC: C12P7/46 ,  C12N1/21 ,  C12N1/18 ,  C12N1/16 ,  C12N15/74

    CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/14 ,  C12P7/46

    Abstract: The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions encoding an enzyme associated with growth-coupled production of succinate when an activity of the enzyme is reduced, whereby the one or more gene disruptions confers stable growth-coupled production of succinate onto the non- naturally occurring microorganism. Also provided is a non-naturally occurring microorganism comprising a set of metabolic modifications obligatory coupling succinate production to growth of the microorganism, the set of metabolic modifications comprising disruption of one or more genes selected from the set of genes comprising: (a) adhE, ldhA; (b) adhE, ldhA, acka-pta; (c) pfl, ldhA; (d) pfl, ldhA, adhE; (e) acka-pta, pykF, atpF, sdhA; (f) acka-pta, pykF, ptsG, or (g) acka-pta, pykF, ptsG, adhE, ldhA, or an ortholog thereof, wherein the microorganism exhibits stable growth-coupled production of succinate. Additionally provided is a non-naturally occurring microorganism having the genes encoding the metabolic modification (e) acka-pta, pykF, atpF, sdhA that further includes disruption of at least one gene selected from pyka, atpH, sdhB or dhaKLM; a non-naturally occurring microorganism having the genes encoding the metabolic modification (f) ackA-pta, pykF, ptsG that further includes disruption of at least one gene selected from pykA or dhaKLM, or a non-naturally occurring microorganism having the genes encoding the metabolic modification (g) ackA-pta, pykF, ptsG, adhE, ldhA that further includes disruption of at least one gene selected from pykA or dhaKLM. The disruptions can be complete gene disruptions and the non-naturally occurring organisms can include a variety of prokaryotic or eukaryotic microorganisms. A method of producing a non-naturally occurring microorganism having stable growth-coupled production of succinate also is provided. The method includes: (a) identifying in silico a set of metabolic modifications requiring succinate production during exponential growth, and (b) genetically modifying a microorganism to contain the set of metabolic modifications requiring succinate production.

    Production of non-native monounsaturated fatty acids in bacteria
    305.
    发明授权
    Production of non-native monounsaturated fatty acids in bacteria 有权

    公开(公告)号：US12221644B2

    公开(公告)日：2025-02-11

    申请号：US17271439

    申请日：2019-08-28

    Applicant: Genomatica, Inc.

    Inventor： Andreas W. Schirmer ,  Katherine Ann Murphy ,  Erin Frances Perry

    IPC: C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12P7/6418 ,  C12P7/6481

    Abstract: The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides viable bacterial cells which comprise heterologous dual 3-hydroxy-acyl-ACP dehydratase/isomerases, etc. The disclosure further provides monounsaturated fatty acid derivative molecules produced by the viable bacterial cells which are non-native to the bacterial cells. The disclosure further provides methods for the preparation and production of non-native monounsaturated fatty acid derivative molecules such as e.g., an ω3-monounsaturated fatty acid derivative, an ω5-monounsaturated fatty acid derivative, an ω9-monounsaturated fatty acid derivative, an ω11-monounsaturated fatty acid fatty acid derivative, etc.

    Method for the Removal of Water from and Transport of Aliphatic Diamines
    309.
    发明公开
    Method for the Removal of Water from and Transport of Aliphatic Diamines 审中-公开

    公开(公告)号：US20240336554A1

    公开(公告)日：2024-10-10

    申请号：US18294035

    申请日：2022-09-19

    Applicant: COVESTRO DEUTSCHLAND AG ,  Genomatica, Inc.

    Inventor： Michael Merkel ,  Mathias LEIMBRINK ,  Friedhelm STEFFENS

    IPC: C07C209/84 ,  C07C263/10 ,  C08G69/28

    CPC classification number: C07C209/84 ,  C07C263/10 ,  C08G69/28 ,  C07C2601/14 ,  C07C2602/42

    Abstract: The invention relates to a method for the removal of water from and transport of at least one aliphatic diamine comprising the following steps: a) providing a first composition including 60 to 98 wt.-% water and 2 to 40 wt.-% of the at least one aliphatic diamine with two —NH2 groups per molecule, each of them bound to a primary or secondary carbon atom, b) removing water at least partially from said first composition by distillation at a first production location in a distillation apparatus comprising at least two distillation columns operating at different head pressures, thus generating a second composition comprising the diamine and ≤55 wt.-% water, wherein the vapours emerging from the distillation column operating at the higher head pressure are used to evaporate the liquid in the bottoms and/or the feed of a distillation column operating at the lower head pressure at least partially, c) transporting said second composition during a span of time t from the first production location to a second production location, wherein the span of time t including times for optional temporary storage is at least 6 h.

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