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    MUTATED NUCLEOTIDE MOLECULE, AND TRANSFORMED PLANT CELLS AND PLANTS COMPRISING THE SAME
    361.
    发明申请
    MUTATED NUCLEOTIDE MOLECULE, AND TRANSFORMED PLANT CELLS AND PLANTS COMPRISING THE SAME 审中-公开
    突变核酸分子和转化植物细胞和包含其的植物

    公开(公告)号：US20150315607A1

    公开(公告)日：2015-11-05

    申请号：US14596419

    申请日：2015-01-14

    Applicant: Academia Sinica

    Inventor： SWEE-SUAK KO ,  MIN-JENG LI

    IPC: C12N15/82 ,  C07K14/415

    CPC classification number: C12N15/8289 ,  C07K14/415

    Abstract: The present invention relates to a method for producing male sterile plant, a mutated nucleotide molecule comprising a nucleotide sequence of the transcription factor bHLH142 and an inserted T-DNA segment, and a novel transformed plant cell and a male-sterile mutant plant comprising the mutated nucleotide molecule, in which the transcription factor bHLH142 is not expressed. The present invention also relates to a novel reversible male sterile transgenic plant, wherein the transcription factor bHLH142 is overexpressed, and its preparation method. The bHLH gene is tissue specifically expresses in the anther and it plays a pivotal role in pollen development. Both the male sterile and reversible male sterile transgenic plants showed a completely male sterile phenotype, but the fertility of the reversible male sterile transgenic plant can be restored under low temperature.

    Abstract translation: 本发明涉及一种生产雄性不育植物的方法，包含转录因子bHLH142的核苷酸序列和插入的T-DNA片段的突变核苷酸分子，以及新的转化植物细胞和雄性不育突变植物，其包含突变的 核苷酸分子，其中转录因子bHLH142未被表达。 本发明还涉及一种新型的可逆雄性不育转基因植物，其中转录因子bHLH142被过表达，其制备方法。 bHLH基因在花药中特异性表达，在花粉发育中起关键作用。 雄性不育和可逆雄性不育转基因植物均显示完全雄性不育表型，但可逆性雄性不育转基因植物的生育力可在低温下恢复。

    Copolymer, complex and method for releasing viruses using pH-dependence of the copolymer
    362.
    发明授权
    Copolymer, complex and method for releasing viruses using pH-dependence of the copolymer 有权
    共聚物，复合物和使用共聚物的pH依赖性释放病毒的方法

    公开(公告)号：US09150688B2

    公开(公告)日：2015-10-06

    申请号：US13459894

    申请日：2012-04-30

    Applicant: Yeu Kuang Hwu ,  S Ja Tseng

    Inventor： Yeu Kuang Hwu ,  S Ja Tseng

    IPC: C08G63/685 ,  C08L67/04 ,  C12N7/00

    CPC classification number: C08G63/685 ,  A61K47/59 ,  A61K47/6901 ,  C12N7/00 ,  C12N2750/14143 ,  C12N2750/14151

    Abstract: A method for releasing viruses includes the steps of: preparing a first negatively charged complex, comprising a plurality of viruses, a plurality of polyethyleneimine particles, and a copolymer; transferring the complex to an acidic region, thereby transforming the complex into a positively charged complex to release a portion of the viruses in the acidic region; and transferring the complex to a non-acidic region, thereby transforming the positively charged complex into a negatively charged complex. One embodiment of the copolymer has the following chemical formula: wherein R1 represents aliphatic compounds or aromatic compounds, and R2 includes at least one negatively charged group.

    Abstract translation: 用于释放病毒的方法包括以下步骤：制备包含多个病毒的第一带负电荷的复合物，多个聚乙烯亚胺颗粒和共聚物; 将复合物转移到酸性区域，从而将络合物转化为带正电荷的络合物以在酸性区域中释放一部分病毒; 并将络合物转移到非酸性区域，从而将带正电的络合物转化为带负电荷的络合物。 共聚物的一个实施方案具有以下化学式：其中R 1表示脂族化合物或芳族化合物，并且R 2包括至少一个带负电荷的基团。

    Optical module for X-ray microscope
    363.
    发明授权
    Optical module for X-ray microscope 有权
    X光显微镜光学模块

    公开(公告)号：US09075245B2

    公开(公告)日：2015-07-07

    申请号：US13733421

    申请日：2013-01-03

    Applicant: ACADEMIA SINICA

    Inventor： En-Te Hwu

    IPC: G02B21/28 ,  G02B7/02 ,  G21K7/00 ,  H01J37/15

    CPC classification number: G02B21/28 ,  G02B7/02 ,  G02B7/028 ,  G21K7/00 ,  H01J37/15

    Abstract: An optical module includes at least a carrying stage, at least an actuator unit and at least an optical assembly. The carrying stage has a first aperture. The actuator unit is disposed at one side of the carrying stage and has a second aperture. The optical assembly is connected with the actuator unit, and the actuator unit adjusts the position of the optical assembly. A radiated wave enters from one side of the optical module and passes through the first aperture, the second aperture and the optical assembly. A microscope with the optical module has compact size and is easily assembled and carried. The optical module and microscope can efficiently reduce the effect of ambient temperature variations so as to improve the measuring stability thereof.

    Abstract translation: 光学模块至少包括载物台，至少一个致动器单元和至少一个光学组件。 承载台具有第一孔。 致动器单元设置在承载台的一侧并且具有第二孔。 光学组件与致动器单元连接，致动器单元调节光学组件的位置。 辐射波从光学模块的一侧进入，并穿过第一孔，第二孔和光学组件。 具有光学模块的显微镜具有紧凑的尺寸并且易于组装和携带。 光学模块和显微镜可以有效地降低环境温度变化的影响，从而提高其测量的稳定性。

    Height measurement by correlating intensity with position of scanning object along optical axis of a structured illumination microscope
    366.
    发明授权
    Height measurement by correlating intensity with position of scanning object along optical axis of a structured illumination microscope 有权
    通过与结构化照明显微镜的光轴相关联的强度与扫描对象的位置进行高度测量

    公开(公告)号：US09068823B2

    公开(公告)日：2015-06-30

    申请号：US14258417

    申请日：2014-04-22

    Applicant: Academia Sinica

    Inventor： Chau-Hwang Lee ,  Chun-Chieh Wang

    IPC: G01B9/02 ,  G01B11/25 ,  G06T7/00 ,  G01B11/06

    CPC classification number: G01B11/25 ,  G01B11/0608 ,  G06T7/521 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30004

    Abstract: A method for imaging an object using a microscope includes obtaining axial response data, the axial response data representative of a relationship between a separation between a top surface of the object and an objective lens of the microscope and an intensity of light reflected by the top surface of the object; positioning the object at a distance from the objective lens that is within a linear region of the axial response data; sequentially illuminating the object with a plurality of periodic patterns; obtaining a plurality of images of the object, each image resulting from the illumination of the object with a corresponding one of the plurality of periodic patterns; determining a reconstructed image of the object based on the plurality of images of the object; and, based on variations in the intensity of the reconstructed image, determining a topographic profile of the top surface of the object.

    Abstract translation: 使用显微镜成像物体的方法包括获得轴向响应数据，轴向响应数据表示物体的顶表面与显微镜的物镜之间的间隔与由顶表面反射的光的强度之间的关系 的对象; 将物体定位在与轴向响应数据的线性区域内的离开物镜的距离处; 以多个周期性图案依次照明物体; 获得所述对象的多个图像，所述每个图像由所述对象的照明产生，所述每个图像具有所述多个周期性图案中的对应的一个; 基于对象的多个图像确定对象的重建图像; 并且基于重建图像的强度的变化，确定对象的顶表面的地形轮廓。

    Abiotic stress tolerant transgenic plants
    368.
    发明授权
    Abiotic stress tolerant transgenic plants 有权
    非生物胁迫耐受转基因植物

    公开(公告)号：US09006535B2

    公开(公告)日：2015-04-14

    申请号：US11942134

    申请日：2007-11-19

    Applicant: Su-May Yu ,  Tuan-Hua David Ho

    Inventor： Su-May Yu ,  Tuan-Hua David Ho

    IPC: A01H1/00 ,  A01H5/00 ,  C12N15/82 ,  A23L1/10

    CPC classification number: C12N15/8237 ,  A23L7/10 ,  C12N15/8271 ,  C12N15/8273

    Abstract: In a transgenic plant, a stress-responsive promoter containing CE3 and ABRE2 from HVA1 ABRC3 and CE1 from HVA22 ABRC1, directs low background expression of a gene under normal growth conditions, but is highly inducible by an abiotic stress, such as abscisic acid, dehydration, salt or cold. Compared with the wild type plant, the transgenic plant that expresses a stress-resistant gene under the control of the stress-responsive promoter is more resistant and/or tolerant to abiotic stresses and exhibits similar or higher growth rate and strength under regular or normal environmental conditions.

    Abstract translation: 在转基因植物中，含有来自HVA1ABRC3的CE3和ABRE2的应激响应启动子和来自HVA22ABRC1的CE1在正常生长条件下指导基因的低背景表达，但是由非生物胁迫如脱落酸，脱水高度诱导 ，盐或冷。 与野生型植物相比，在胁迫响应启动子控制下表达抗胁迫基因的转基因植物对非生物胁迫具有更强的抗性和/或耐受性，并且在常规或正常环境下表现出相似或更高的生长速率和强度 条件。

    ANTI-EPITHELIAL CELL ADHESION MOLECULE (EpCAM) ANTIBODIES AND METHODS OF USE THEREOF
    369.
    发明申请
    ANTI-EPITHELIAL CELL ADHESION MOLECULE (EpCAM) ANTIBODIES AND METHODS OF USE THEREOF 有权
    抗上皮细胞粘附分子（EpCAM）抗体及其使用方法

    公开(公告)号：US20150017230A1

    公开(公告)日：2015-01-15

    申请号：US14381362

    申请日：2013-03-01

    Applicant: ACADEMIA SINICA

    Inventor： Han-Chung Wu ,  Mei-Ying Liao ,  Cheng-Wei Lin

    IPC: C07K16/18 ,  G01N33/574

    CPC classification number: C07K16/18 ,  A61K2039/505 ,  C07K16/30 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/574

    Abstract: An isolated monoclonal antibody or an antigen-binding fragment thereof is disclosed. The antibody or the antigen-binding fragment is characterized by: (a) having a specific binding affinity to epithelial cell adhesion molecule (EpCAM) comprising the amino acid sequence of SEQ ID NO: 1; (b) having a specific binding affinity to cancer cells expressing EpCAM said cancer cells being selected from the group consisting of oral cancer cells, nasopharyngeal cancer cells (NPC), colorectal cancer cells, and ovarian cancer cells; and (c) having no binding affinity to human umbilical vein endothelial cell (HUVEC) and normal nasal mucosal epithelia (NNM). Also disclosed is an isolated monoclonal antibody or an antigen-binding fragment thereof that has a specific binding affinity to an epitope within the sequence of KPEGALQNNDGLYDPDCDE (SEQ ID NO: 63) located within the EGF-like domain II of epithelial cell adhesion molecule (EpCAM). Methods of using the same are also disclosed.

    Abstract translation: 公开了分离的单克隆抗体或其抗原结合片段。 抗体或抗原结合片段的特征在于：（a）对包含SEQ ID NO：1的氨基酸序列的上皮细胞粘附分子（EpCAM）具有特异性结合亲和力; （b）对表达EpCAM的癌细胞具有特异性结合亲和力，所述癌细胞选自口腔癌细胞，鼻咽癌细胞（NPC），结肠直肠癌细胞和卵巢癌细胞; 和（c）对人脐静脉内皮细胞（HUVEC）和正常鼻粘膜上皮细胞（NNM）没有结合亲和力。 还公开了与位于上皮细胞粘附分子（EpCAM）的EGF样结构域II内的KPEGALQNNDGLYDPDCDE（SEQ ID NO：63）的序列内的表位具有特异性结合亲和力的分离的单克隆抗体或其抗原结合片段 ）。 还公开了使用该方法的方法。

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