公开(公告)号：US20240261705A1

公开(公告)日：2024-08-08

申请号：US18566551

申请日：2022-06-27

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Asa Hagner McWhirter ,  Ola Lind ,  Frederic Leroux

IPC: B01D15/36 ,  B01J41/20 ,  C12N7/02

CPC classification number: B01D15/363 ,  B01J41/20 ,  C12N7/02

Abstract: An anion exchange chromatography medium (1) for use in purification of enveloped virus particles or exosomes from a feed, the anion exchange chromatography medium comprising a support material being functionalized with a ligand comprising a diamine functionality generating at least one weak anion exchange group to an ionic capacity of 10-500 μmol/mL.

公开(公告)号：US20240226357A1

公开(公告)日：2024-07-11

申请号：US18604231

申请日：2024-03-13

Applicant: Cytiva BioProcess R&D AB

Inventor： Elin Marianne Monie ,  Tomas Bjorkman ,  Anna Gronberg ,  Anders Ljunglof ,  Gustav Jose Rodrigo ,  Karin Torstenson ,  Magnus Carl Erik Wetterhall

IPC: A61L2/18 ,  B01D15/20 ,  B01D15/38 ,  G01N30/50

CPC classification number: A61L2/18 ,  B01D15/203 ,  B01D15/3804 ,  G01N30/50

Abstract: The invention discloses a method for cleaning or sanitization of an affinity chromatography matrix, comprising the steps of:
a) providing an affinity chromatography matrix having oxidation-tolerant proteinaceous ligands coupled to a support,
b) contacting the matrix with a sanitization solution comprising at least one oxidant defined by formula I,




wherein R is hydrogen or an acyl group R′—C(O)—, with R′ being a hydrogen or a methyl, ethyl or propyl group.

公开(公告)号：US20240076650A1

公开(公告)日：2024-03-07

申请号：US18260560

申请日：2022-01-26

Applicant: CYTIVA BIOPROCESS R&D AB

Inventor： Peter Lundback ,  Ronnie Palmgren ,  Ola Lind ,  Nils Norrman ,  Helen Cheek ,  Susana Brito Dos Santos ,  Per Denker

IPC: C12N15/10 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/289

CPC classification number: C12N15/101 ,  B01D15/3819 ,  B01J20/265 ,  B01J20/28035 ,  B01J20/28038 ,  B01J20/289 ,  B01J2220/52

Abstract: The invention relates to processes for purification of poly-tagged products, such as mRNA, from synthetic or biological compositions. The process involves contacting the composition with a oligo d (T)-functionalized chromatography medium comprising a convection-based chromatography material.

公开(公告)号：US20230391850A1

公开(公告)日：2023-12-07

申请号：US18249525

申请日：2021-11-10

Applicant: Cytiva BioProcess R&D AB

Inventor： Mats Ander ,  Gustav Rodrigo ,  Tomas Björkman

IPC: C07K16/06 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32 ,  B01J20/28 ,  C07K14/31

CPC classification number: C07K16/065 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3212 ,  B01J20/3274 ,  B01J20/28038 ,  C07K14/31

Abstract: The invention relates to an alkali-stable mutated Fc-binding Protein A domain, having at least 95% identity to SEQ ID NO: 8 or SEQ ED NO: 9.

公开(公告)号：US11807666B2

公开(公告)日：2023-11-07

申请号：US18059929

申请日：2022-11-29

Applicant: Cytiva BioProcess R&D AB

Inventor： Martin Hall ,  Karolina Busson ,  Jean-Luc Maloisel ,  Helena Skoglar

IPC: C07K1/18 ,  B01J41/20 ,  B01D15/36 ,  B01D15/38 ,  A61K38/36 ,  A61K38/48 ,  A61K35/16 ,  C12N9/64 ,  B01D15/34 ,  C07K14/47 ,  C07K14/755 ,  C07K14/765 ,  C07K16/00 ,  A61K38/00

CPC classification number: C07K1/18 ,  A61K35/16 ,  A61K38/36 ,  A61K38/4846 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3847 ,  B01J41/20 ,  C07K14/472 ,  C07K14/755 ,  C07K14/765 ,  C07K16/00 ,  C12N9/644 ,  C12N9/6424 ,  C12Y304/21022 ,  A61K38/00

Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of proteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.

公开(公告)号：US11685764B2

公开(公告)日：2023-06-27

申请号：US17221438

申请日：2021-04-02

Applicant: Cytiva BioProcess R&D AB

Inventor： Gustav José Rodrigo ,  Tomas Bjorkman ,  Mats Ander ,  Jesper Ulf Hansson

IPC: A23J1/00 ,  C07K1/22 ,  B01D15/38 ,  B01J20/24 ,  B01J20/28 ,  B01J20/286 ,  B01J20/32 ,  C07K14/31 ,  C07K16/00 ,  C07K16/06 ,  C07K17/10

CPC classification number: C07K1/22 ,  B01D15/3809 ,  B01J20/24 ,  B01J20/286 ,  B01J20/28016 ,  B01J20/28078 ,  B01J20/3212 ,  B01J20/3274 ,  C07K14/31 ,  C07K16/00 ,  C07K16/065 ,  C07K17/10

Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein:



a) the ligands comprise multimers of alkali-stabilized Protein A domains, and
b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50, v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.

公开(公告)号：US11680081B2

公开(公告)日：2023-06-20

申请号：US16955119

申请日：2018-12-13

Applicant: Cytiva Bioprocess R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Ronnie Palmgren ,  Tomas Bjorkman

IPC: C07K1/22 ,  B01D15/38 ,  B01J20/24 ,  B01J20/28 ,  B01J20/288 ,  B01J20/30 ,  B01J20/32 ,  C07K16/00

CPC classification number: C07K1/22 ,  B01D15/3809 ,  B01J20/24 ,  B01J20/288 ,  B01J20/28016 ,  B01J20/3071 ,  B01J20/3212 ,  B01J20/3217 ,  B01J20/3274 ,  B01J20/3295 ,  C07K16/00 ,  B01J2220/54

Abstract: Methods that include providing and reacting a solid support and an alkali-stable ligand derived from an immunoglobulin-binding bacterial protein to form a separation matrix having covalently coupled alkali-stable ligands; and washing with a wash solution comprising at least 10 mM of an alkali metal hydroxide.

公开(公告)号：US11623941B2

公开(公告)日：2023-04-11

申请号：US16884475

申请日：2020-05-27

Applicant: Cytiva BioProcess R&D AB

Inventor： Annika Kristina Forss ,  Gustav Jose Rodrigo ,  Tomas Bjorkman ,  Jesper Ulf Hansson ,  Mats Ander

IPC: C07K1/22 ,  B01D15/08 ,  C07K16/12 ,  C07K17/10 ,  B01J20/286 ,  C07K14/31 ,  C07K16/06 ,  B01D15/00 ,  B01J20/32

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 51 or SEQ ID NO: 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO: 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO: 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.

公开(公告)号：US20220362744A1

公开(公告)日：2022-11-17

申请号：US17853514

申请日：2022-06-29

Applicant: Cytiva BioProcess R&D AB

Inventor： Ronnie Palmgren ,  Jean-Luc Maloisel ,  Gustav Rodrigo ,  Tomas Bjorkman

IPC: B01J20/286 ,  B01D15/18 ,  B01D15/38 ,  B01J20/28 ,  B01J20/32 ,  C07K1/22

Abstract: A separation matrix comprising porous particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is above 5 mg/ml, the volume-weighted median diameter of said porous particles is at least 10 and below 30 μm and the said porous particles have a gel phase distribution coefficient, expressed as KD for dextran of molecular weight 110 kDa, of 0.5-0.9.

公开(公告)号：US20220219141A1

公开(公告)日：2022-07-14

申请号：US17605502

申请日：2020-04-28

Applicant: Cytiva Bioprocess R&D AB

Inventor： Susanna Lindberg ,  Jonas Gustafsson ,  Linn Carlsson ,  Anders Hagvall ,  Erik Ringberg ,  David Jansson

IPC: B01J20/291 ,  B01J20/24 ,  B01J20/28 ,  B01J20/30

Abstract: The invention discloses method for manufacturing agar or agarose beads, comprising the steps of: a) providing a water phase comprising an aqueous solution of agar or agarose at a temperature of 40-100° C.: b) providing an oil phase comprising a water-immiscible solvent and an emulsifier at a temperature of 40-100° C.; c) emulsifying the water phase in the oil phase to form a water-in-oil emulsion: d) cooling the water-in-oil emulsion to a temperature below a gelation temperature of the agar or agarose to form a dispersion of solidified agar or agarose beads: and c) recovering agar or agarose beads from dispersion, wherein the emulsifier comprises a phosphate ester of an alkoxy lated fatty alcohol.