公开(公告)号：US20220362170A1

公开(公告)日：2022-11-17

申请号：US17774229

申请日：2020-11-06

申请人： AMYRIS, INC.

发明人： Diva CHAN ,  Ro OTERI ,  Ashlee NUNES

IPC分类号： A61K31/05 ,  A61K31/352 ,  A61K31/60 ,  A61K47/06 ,  A61K9/00

摘要： Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject. The compositions contain a carrier that facilitates the delivery and permeation of cannabinoids to and through the skin. The carriers of the compositions contain squalane or mixtures of squalane and hemisqualane. The compositions described herein may serve as the base for the preparation of various cosmetics or various pharmaceuticals. The cosmetics or pharmaceuticals may be used to reduce inflammation, control pain, or prevent aging of the skin of a subject.

公开(公告)号：US20220307059A1

公开(公告)日：2022-09-29

申请号：US17616843

申请日：2020-06-05

申请人： AMYRIS, INC.

发明人： Penelope R. CHUA ,  Joshua Adam LERMAN ,  Thomas Jon SCHERBART ,  Chandresh THAKKER ,  Annie Ening TSONG ,  Hanxiao JIANG

IPC分类号： C12P5/00 ,  C12N1/18 ,  C12N15/81 ,  C12N9/02 ,  C12N9/14

摘要： Provided herein are compositions and methods for uncoupling the yield and productivity of an isoprenoid compound produced in a host cell. In some embodiments, the yield and productivity are uncoupled by genetically modifying the host cell to reduce flux through the citric acid cycle (TCA). In other embodiments, the yield and productivity are uncoupled by reducing the levels of ATP in the host cell.

公开(公告)号：US20220251606A1

公开(公告)日：2022-08-11

申请号：US17690429

申请日：2022-03-09

申请人： AMYRIS, INC.

发明人： Kevin GEORGE ,  Andrew MAIN ,  Chia-Hong TSAI

IPC分类号： C12N15/90 ,  C12N15/10 ,  C12N15/81

摘要： Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.

公开(公告)号：US20220127620A1

公开(公告)日：2022-04-28

申请号：US17438810

申请日：2020-03-13

申请人： Andrew P. KLEIN ,  Amyris, Inc.

发明人： Christopher D. REEVES ,  Christopher J. PADDON ,  Victor F. HOLMES ,  Andrew P. KLEIN

IPC分类号： C12N15/52 ,  C12N1/18 ,  C12N9/10 ,  C12N9/88 ,  C12P7/42

摘要： Provide are modified host cells that are engineered to decrese expression of a product to undetectable levels in the presence of an exogenous agent, and increase expression of the product in the presence of another exogenous agent. The modified yeast strainshost cells do not express detectable levels of a precursor or substrate used to make the product. The product can be a cannabinoid or precursor thereof, and the substrate can be hexanoate. Also provided are methods for making a product using the modified host cells. The modified host cell can be a yeast strain, such as S. cerevisiae.

公开(公告)号：US20220002735A1

公开(公告)日：2022-01-06

申请号：US17371678

申请日：2021-07-09

申请人： AMYRIS, INC.

发明人： Andrew MAIN ,  Grzegorz WOJCIECHOWSKI ,  Yue YANG ,  Lishan ZHAO

IPC分类号： C12N15/52 ,  C12P5/00 ,  C12N1/20 ,  C12N1/14 ,  C12N1/16

摘要： Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.

公开(公告)号：US20210230641A1

公开(公告)日：2021-07-29

申请号：US17255031

申请日：2019-06-27

申请人： AMYRIS, INC.

发明人： Ronald Ray HENRY ,  Joshua Steven LENG

IPC分类号： C12P5/02

摘要： A method for recovering one or more water immiscible compounds comprising acidifying and disrupting the microbial biomass; heating the acidified, disrupted microbial biomass to form a heated, acidified disrupted microbial biomass; and contacting the heated, acidified, disrupted microbial biomass with a disulfonated surfactant in an amount sufficient to release at least 30% of the one or more water immiscible compounds from the microbial biomass.

公开(公告)号：US20210230640A1

公开(公告)日：2021-07-29

申请号：US17255348

申请日：2019-07-11

申请人： AMYRIS, INC.

发明人： Joshua LENG

IPC分类号： C12P5/00 ,  C12P7/64 ,  C12P7/24

摘要： The present disclosure relates to methods for enhancing productivity or yield in fermentation. The methods provide monitoring one or more compounds produced by culture cells as a feedback control for the feed rate of the fermentation.

公开(公告)号：US20200263205A1

公开(公告)日：2020-08-20

申请号：US16646028

申请日：2018-09-12

申请人： AMYRIS, INC.

发明人： Jessica WALTER

IPC分类号： C12N15/90 ,  C12N15/81 ,  C12N15/11 ,  C12N9/22

摘要： The present invention provides high efficiency targeted and marker-less single or simultaneous multiple integrations using nucleases and a stable plasmid in Kluyveromyces host cells.

公开(公告)号：US20200208163A1

公开(公告)日：2020-07-02

申请号：US16646001

申请日：2018-09-12

申请人： Amyris, Inc.

发明人： Hanxiao JIANG

IPC分类号： C12N15/81 ,  C12N15/90

摘要： The present invention provides high efficiency targeted and marker-less single, double, triple, quadruple, and quintuple integrations by using CRISPR in host cells, including Pichia.

公开(公告)号：US10183901B2

公开(公告)日：2019-01-22

申请号：US15458907

申请日：2017-03-14

申请人： AMYRIS, INC.

发明人： Nicholas L. Ohler ,  Roberto Vazquez

IPC分类号： C07C1/00 ,  C07C5/03 ,  C07C7/20 ,  C07C11/21 ,  C10G45/00 ,  C10G53/04 ,  C10G53/08 ,  C10G67/04 ,  C10G67/06 ,  C10L1/04 ,  C10L1/16 ,  C10L1/183

摘要： Methods and compositions for stabilization and subsequent hydrogenation of a microbial-derived immiscible olefin are described. The methods comprise separating immiscible olefin from a mixture comprising an aqueous solution, microbial cells and immiscible olefin thereby forming a crude olefin composition; purifying the crude olefin composition thereby forming a purified olefin composition; and adding a phenolic antioxidant to the purified olefin composition wherein the phenolic antioxidant is a phenol derivative containing an unfused phenyl ring with one or more hydroxyl substituents. The methods further comprise reacting the purified olefin composition with hydrogen in the presence of a hydrogen catalyst such that hydrogen saturates at least one double bond in the olefin. Hydrogenated compositions produced by the methods are further provided.