公开(公告)号：US20240384315A1

公开(公告)日：2024-11-21

申请号：US18688617

申请日：2022-09-02

Applicant: Amyris, Inc.

Inventor： Dominic PINEL ,  Victoria HSIAO ,  Diana Bahraini MARINA ,  Derek A. ABBOTT

IPC: C12P19/00 ,  A23L33/00 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/22 ,  C12N9/88 ,  C12N15/11 ,  C12N15/113 ,  C12N15/81

Abstract: Provided herein are host cells capable of producing a human milk oligosaccharide (HMO), such as yeast cells that are deficient in expression or activity of an endogenous oxidoreductase. Also provided are fermentation compositions including the disclosed host cells, as well as related methods of producing and recovering HMOs generated by the host cells.

公开(公告)号：US20240327875A1

公开(公告)日：2024-10-03

申请号：US18578609

申请日：2022-07-15

Applicant: AMYRIS, INC.

Inventor： Quinn Mitrovich ,  William E. Draper ,  Andrew Klein ,  Michelle Medina

IPC: C12P7/04 ,  C12N1/16 ,  C12N9/16 ,  C12N15/81 ,  C12R1/865

CPC classification number: C12P7/04 ,  C12N1/165 ,  C12N9/16 ,  C12N15/81 ,  C12N2510/00 ,  C12R2001/865 ,  C12Y306/01

Abstract: The present disclosure features compositions and methods for producing one or more isoprenoid compounds, such as E-copalol, in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of an isoprenoid biosynthetic pathway, such as a pathway for making E-copalol. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of an isoprenoid biosynthetic pathway, such as a copalyl-diphosphate (CPP) pyrophosphatase. The host cell may then be cultured in a medium, for example, in the presence of an agent that regulates expression of the one or more enzymes. The host cell may further be incubated for a time sufficient to allow for production of an isoprenoid compound, such as E-copalol, by the host cell. The isoprenoid compound may then be separated from the host cell or from the medium.

公开(公告)号：US20240035061A1

公开(公告)日：2024-02-01

申请号：US18208030

申请日：2023-06-09

Applicant: AMYRIS, INC.

Inventor： Hiroko Tsuruta ,  Jacob R. Lenihan ,  Rika Regentin

IPC: C12P23/00 ,  C12N1/32 ,  C12P5/00

CPC classification number: C12P23/00 ,  C12N1/32 ,  C12P5/007 ,  Y02E50/10

Abstract: Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells.

公开(公告)号：US11884928B2

公开(公告)日：2024-01-30

申请号：US16646013

申请日：2018-09-12

Applicant: Amyris, Inc.

Inventor： Yoseph Tsegaye

IPC: C12N9/22 ,  C12N15/62 ,  C12N15/113 ,  C12N15/81 ,  C07K16/28 ,  C07K16/32

CPC classification number: C12N15/815 ,  C07K16/2887 ,  C07K16/32 ,  C12N9/22 ,  C12N15/113 ,  C12N2310/20 ,  C12N2800/80

Abstract: The invention provides methods for genetically engineering Kluyveromyces. The methods can be used to genetically engineer Kluyveromyces to produce and secrete full-length antibodies or antibody fragments. The invention also provides methods for production and secretion of antibodies.

公开(公告)号：US20230304049A1

公开(公告)日：2023-09-28

申请号：US18022063

申请日：2021-08-19

Applicant: Amyris, Inc.

Inventor： Victor F. HOLMES ,  John E. HUNG ,  Irina A. KORYAKINA ,  Andrew P. KLEIN ,  Yi XIONG ,  William E. DRAPER ,  Hailley S. WARBINGTON

IPC: C12P7/42 ,  C12N9/02 ,  C12N9/10 ,  C12N9/00 ,  C12N9/88 ,  C12N1/18

CPC classification number: C12P7/42 ,  C12N9/0004 ,  C12N9/1029 ,  C12N9/93 ,  C12N9/88 ,  C12Y404/01026 ,  C12Y121/03008 ,  C12N1/185

Abstract: The compositions and methods of the disclosure can be used to produce a cannabinoid in a host cell, such as a yeast cell. For example, the disclosure features host cells (e.g., yeast cells) modified to express one or more enzymes of a cannabinoid biosynthetic pathway, such as an acyl activating enzyme (AAE), a tetraketide synthase (TKS), a cannabigerolic acid synthase (CBGaS), and/or an olivetolic acid cyclase (OAC).

公开(公告)号：US20230270639A1

公开(公告)日：2023-08-31

申请号：US18012493

申请日：2021-06-30

Applicant: Amyris, Inc.

Inventor： CHRISTA LYNNA MARIANO ,  RAMYA VISWANATHAN

IPC: A61K8/31 ,  A61Q17/04 ,  A61K8/37

CPC classification number: A61K8/31 ,  A61Q17/04 ,  A61K8/37 ,  A61K2800/522

Abstract: Provided herein are sunscreen formulations containing a metal oxide sunscreen, squalane, and an antioxidant. The sunscreen formulations provided have increased SPF per unit mass of the metal oxide compared to sunscreen formulations that contain metal oxide without the addition of squalane and antioxidant. Also provided are methods of preventing UV damages to skin involving the application of the sunscreen formulation.

公开(公告)号：US20220325303A1

公开(公告)日：2022-10-13

申请号：US17840369

申请日：2022-06-14

Applicant: AMYRIS, INC.

Inventor： Andrew HORWITZ ,  Kristy Michelle HAWKINS ,  Max SCHUBERT ,  Wayne SZETO

IPC: C12N15/90 ,  C12Q1/6897 ,  C12N15/11

Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.

公开(公告)号：US20220154092A1

公开(公告)日：2022-05-19

申请号：US17518540

申请日：2021-11-03

Applicant: Amyris, Inc.

Inventor： Nicholas Ohler ,  Karl Fisher ,  Jin Ki Hong

IPC: C10M105/02 ,  C10L1/04 ,  C10M105/04 ,  C10M107/14 ,  C07C5/05 ,  C07C5/03

Abstract: Provided herein are olefinic feedstocks derived from conjugated hydrocarbon terpenes (e.g., C10-C50 terpenes), methods for making the same, and methods for their use.

公开(公告)号：US11312976B2

公开(公告)日：2022-04-26

申请号：US17255031

申请日：2019-06-27

Applicant: AMYRIS, INC.

Inventor： Ronald Ray Henry ,  Joshua Steven Leng

IPC: C12P5/02 ,  C12N1/18 ,  C12R1/865

Abstract: A method for recovering one or more water immiscible compounds comprising acidifying and disrupting the microbial biomass; heating the acidified, disrupted microbial biomass to form a heated, acidified disrupted microbial biomass; and contacting the heated, acidified, disrupted microbial biomass with a disulfonated surfactant in an amount sufficient to release at least 30% of the one or more water immiscible compounds from the microbial biomass.

公开(公告)号：US11230716B2

公开(公告)日：2022-01-25

申请号：US16733191

申请日：2020-01-02

Applicant: Amyris, Inc. ,  Total Marketing Services

Inventor： Penelope R. Chua ,  Adam Meadows

IPC: C12N1/20 ,  C12N9/00 ,  C12N15/00 ,  C12N15/81 ,  C12N15/63 ,  C12N1/16 ,  C12P5/00

Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.