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53 条记录 (耗时 0.026 秒)

    Reagents for intracellular delivery of macromolecules
    34.
    发明授权
    Reagents for intracellular delivery of macromolecules 失效
    细胞内分泌大分子的试剂

    公开(公告)号：US06075012A

    公开(公告)日：2000-06-13

    申请号：US195866

    申请日：1994-02-11

    申请人： Gulilat Gebeyehu ,  Joel A. Jessee

    发明人： Gulilat Gebeyehu ,  Joel A. Jessee

    IPC分类号： A61K48/00

    CPC分类号： A61K48/00 ,  A61K47/48046

    摘要： The present invention discloses cationic lipids and lipophilic compounds useful for making lipid aggregates for delivery of macromolecules and other compounds into cells. They are especially useful for the DNA-dependent transformation of cells. Also disclosed are compositions of cationic lipids and viral components or non-viral fusagenic compounds useful for enhancing transfection.

    摘要翻译： 本发明公开了用于制备用于将大分子和其它化合物递送到细胞中的脂质聚集体的阳离子脂质和亲脂性化合物。 它们对细胞的DNA依赖性转化特别有用。 还公开了阳离子脂质和病毒组分的组合物或可用于增强转染的非病毒成骨细胞化合物。

    METHOD FOR DEBLOCKING OF LABELED OLIGONUCLEOTIDES
    37.
    发明申请
    METHOD FOR DEBLOCKING OF LABELED OLIGONUCLEOTIDES 有权
    用于解锁标记的寡核苷酸的方法

    公开(公告)号：US20120253028A1

    公开(公告)日：2012-10-04

    申请号：US13152907

    申请日：2011-06-03

    申请人： Gulilat GEBEYEHU ,  Richard M. Pires

    发明人： Gulilat GEBEYEHU ,  Richard M. Pires

    IPC分类号： C07H21/04

    CPC分类号： C07H21/00 ,  C07B2200/11 ,  C12Q1/68

    摘要： The invention relates to a process for deblocking substantially a blocked, detectably labeled oligonucleotide by contacting the blocked detectably labeled oligonucleotide with an effective amount of a nucleophilic amino compound under conditions that result in substantial deblocking of the oligonucleotide, thereby giving the substantially deblocked oligonucleotide.

    摘要翻译： 本发明涉及一种通过使阻断的可检测标记的寡核苷酸与有效量的亲核性氨基化合物在导致寡核苷酸的实际解锁的条件下接触来封闭基本上封闭的可检测标记的寡核苷酸的方法，从而得到基本上解锁的寡核苷酸。

    Method for deblocking of labeled oligonucleotides
    38.
    发明授权
    Method for deblocking of labeled oligonucleotides 有权
    标记寡核苷酸解封的方法

    公开(公告)号：US08093372B2

    公开(公告)日：2012-01-10

    申请号：US10365644

    申请日：2003-02-13

    申请人： Gulilat Gebeyehu ,  Richard M Pires

    发明人： Gulilat Gebeyehu ,  Richard M Pires

    IPC分类号： C07H21/00

    CPC分类号： C07H21/00 ,  C07B2200/11 ,  C12Q1/68

    摘要： The invention relates to a process for deblocking substantially a blocked, detectably labeled oligonucleotide by contacting the blocked detectably labeled oligonucleotide with an effective amount of a nucleophilic amino compound under conditions that result in substantial deblocking of the oligonucleotide, thereby giving the substantially deblocked oligonucleotide.

    摘要翻译： 本发明涉及一种通过使阻断的可检测标记的寡核苷酸与有效量的亲核性氨基化合物在导致寡核苷酸的实际解锁的条件下接触来封闭基本上封闭的可检测标记的寡核苷酸的方法，从而得到基本上解锁的寡核苷酸。

    TRANSFECTION REAGENTS
    40.
    发明申请
    TRANSFECTION REAGENTS 有权

    公开(公告)号：US20100159593A1

    公开(公告)日：2010-06-24

    申请号：US12638336

    申请日：2009-12-15

    申请人： Yongliang CHU ,  Malek Masoud ,  Gulilat Gebeyehu

    发明人： Yongliang CHU ,  Malek Masoud ,  Gulilat Gebeyehu

    IPC分类号： C12N5/02 ,  C07D403/12 ,  C07C233/49

    CPC分类号： A61K47/48046 ,  A61K9/16 ,  A61K31/14 ,  A61K38/28 ,  A61K38/40 ,  A61K47/543 ,  C07C211/21 ,  C07C211/63 ,  C07C211/64 ,  C07C229/26 ,  C07D209/48 ,  C07D241/12 ,  C12N15/88 ,  Y10S435/81

    摘要： Disclosed are compounds capable of facilitating transport of biologically active agents or substances into cells having the general structure: wherein Q is selected from the group consisting of N, O and S; L is any bivalent organic radical capable of linking each Q, such as C, CH, (CH2)l, or {(CH2)i-Y—(CH2)j}k, wherein Y is selected from the group consisting of CH2, an ether, a polyether, an amide, a polyamide, an ester, a sulfide, a urea, a thiourea, a guanidyl, a carbamoyl, a carbonate, a phosphate, a sulfate, a sulfoxide, an imine, a carbonyl, and a secondary amino group and wherein Y is optionally substituted by —X1-L′-X2—Z or —Z; R1-R6, independently of one another, are selected from the group consisting of H, —(CH2)p-D-Z, an alkyl, an alkenyl, an aryl, and an alkyl or alkyl ether optionally substituted by one or more of an alcohol, an aminoalcohol, an amine, an amide, an ether, a polyether, a polyamide, an ester, a mercaptan, an alkylthio, a urea, a thiourea, a guanidyl, or a carbamoyl group, and wherein at least one of R1, R3, R4 and R6 is a straight chain or branched, cyclic, alkyl, alkenyl, alkynyl or aryl group; and anyone of R1, R3, R4 and/or R6 may optionally be covalently linked with each other, with Y or with L when L is C or CH to form a cyclic moiety; Z is selected from the group consisting of amine, spermiyl, carboxyspermiyl, guanidyl, spermidinyl, putricinyl, diaminoalkyl, pyridyl, piperidinyl, pyrrolidinyl, polyamine, amino acid, peptide, and protein; X1 and X2, independently of one another, are selected from the group consisting of NH, O, S, alkylene, and arylene; L′ is selected from the group consisting of alkylene, alkenylene, alkynylene, arylene, alkylene ether, and polyether; D is Q or a bond; A1 and A2, independently of one another, are selected from the group consisting of CH2O, CH2S, CH2NH, C(O), C{NH), C(S) and (CH2)t; X is a physiologically acceptable anion; m, n, r, s, u, v, w and y are 0 or 1, with the proviso that when both m and n are 0 at least one of r, s, u and y is other than 0; i, j, k, l, p and are integers from 0 to about 100; q is an integer from 1 to about 1000; and a is the number of positive charge divided by the valence of the anion.