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    Regulatory element for heterologous protein production in the fruiting body of filamentous fungi
    32.
    发明授权
    Regulatory element for heterologous protein production in the fruiting body of filamentous fungi 有权

    公开(公告)号：US10131916B2

    公开(公告)日：2018-11-20

    申请号：US14851819

    申请日：2015-09-11

    Applicant: Intrexon Corporation

    Inventor： Zhixin Shi ,  Jack Q. Wilkinson ,  Donald S. Walters ,  C. Peter Romaine

    IPC: C12N15/63 ,  C12N15/80

    Abstract: The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the Agaricus bisporus lectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.

    Methods for Dynamic Vector Assembly of DNA Cloning Vector Plasmids
    33.
    发明申请
    Methods for Dynamic Vector Assembly of DNA Cloning Vector Plasmids 审中-公开

    公开(公告)号：US20180023089A1

    公开(公告)日：2018-01-25

    申请号：US14825195

    申请日：2015-08-13

    Applicant: Intrexon Corporation

    Inventor： Thomas D. REED

    IPC: C12N15/64 ,  C12N15/85 ,  C12N15/66 ,  C07H21/04

    CPC classification number: C12N15/64 ,  C07H21/04 ,  C12N15/66 ,  C12N15/85

    Abstract: A method for using cloning vector plasmids to produce DNA molecules, such as transgenes, in a single cloning step. The transgenes can be used for the purpose of gene expression or analysis of gene expression. The plasmid cloning vectors are engineered to minimize the amount of manipulation of DNA fragment components by the end user of the vectors and the methods for their use. Transgenes produced using the invention may be used in a single organism, or in a variety of organisms including bacteria, yeast, mice, and other eukaryotes with little or no further modification.

    Modified forms of Pseudomonas exotoxin A
    36.
    发明授权
    Modified forms of Pseudomonas exotoxin A 有权

    公开(公告)号：US09677055B2

    公开(公告)日：2017-06-13

    申请号：US15234297

    申请日：2016-08-11

    Applicant: Intrexon Corporation

    Inventor： Timothy David Jones ,  Francis Joseph Carr

    IPC: C12N9/10

    CPC classification number: C12N9/1077 ,  A61K38/00 ,  A61K39/00 ,  A61K39/02 ,  C07K14/21 ,  C07K16/2803 ,  C07K2317/622 ,  C12Y204/02036

    Abstract: Pseudomonas exotoxin A or “PE” is a 66 kD, highly potent, cytotoxic protein secreted by the bacterium Pseudomonas aeruginosa. Various forms of PE have been coupled to other proteins, such as antibodies, to generate therapeutically useful cytotoxin conjugates that selectively target cells of a desired phenotype (such as tumor cells). In the present invention, peptides spanning the sequence of an approximately 38 kD form of Pseudomonas exotoxin A protein were analyzed for the presence of immunogenic CD4+ T cell epitopes. Six immunogenic T cell epitopes were identified. Residues were identified within each epitope for introduction of targeted amino acid substitutions to reduce or prevent immunogenic T-cell responses in PE molecules which may be administered to a heterologous host.

    Endoplasmic reticulum localization signals
    38.
    发明授权
    Endoplasmic reticulum localization signals 有权

    公开(公告)号：US09617317B2

    公开(公告)日：2017-04-11

    申请号：US14850990

    申请日：2015-09-11

    Applicant: Intrexon Corporation

    Inventor： Thomas D. Reed

    IPC: C07K14/47 ,  A01K67/027 ,  C12N15/62 ,  C12N15/85 ,  C07K2/00

    CPC classification number: C07K14/47 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  C07K2/00 ,  C07K2319/04 ,  C12N15/625 ,  C12N15/8509

    Abstract: The invention relates to cellular localization signals. In particular, the invention relates to endoplasmic reticulum localization signals in monomeric or multimeric form. The localization signals are utilized as research tools or are linked to therapeutics. Disclosed are methods of making and using polypeptides and modified polypeptides as signals to localize therapeutics, experimental compounds, peptides, proteins and/or other macromolecules to the endoplasmic reticulum of eukaryotic cells. The polypeptides of the invention optionally include linkage to reporters, epitopes and/or other experimental or therapeutic molecules. The invention also encompasses polynucleotides encoding the localization signals and vectors comprising these polynucleotides.

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