公开(公告)号：US20230160008A1

公开(公告)日：2023-05-25

申请号：US18100133

申请日：2023-01-23

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

CPC classification number: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20220282329A1

公开(公告)日：2022-09-08

申请号：US17707189

申请日：2022-03-29

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US12286673B2

公开(公告)日：2025-04-29

申请号：US18340511

申请日：2023-06-23

Applicant: 10x Genomics, Inc.

Inventor： Felice Alessio Bava

IPC: C12Q1/6874 ,  C12N15/10

Abstract: Disclosed herein are methods of amplifying an analyte in a biological sample using a bridging oligonucleotide that hybridizes to a captured analyte. The methods disclosed herein include steps of (a) contacting a biological sample with a substrate having capture probes comprising a capture domain and a spatial barcode; (b) hybridizing the analyte to the capture domain; and (c) contacting the analyte to a bridging oligonucleotide comprising (i) a capture-probe-binding sequence, and (ii) an analyte-binding sequence; (d) extending the bridging oligonucleotide; and (e) determining (i) all or a part of the sequence of the analyte, or a complement thereof, and (ii) the spatial barcode, or a complement thereof, and using the determined sequence of (i) and (ii) to determine the location of the analyte in the biological sample.

公开(公告)号：US20250129412A1

公开(公告)日：2025-04-24

申请号：US18972221

申请日：2024-12-06

Applicant: 10x Genomics, Inc.

Inventor： Cedric Uytingco ,  Zachary Bent ,  Eswar Prasad Ramachandran Iyer ,  Fernandino Valdecanas ,  Meghan L. F. Frey ,  Steven William Short ,  Yifeng Yin ,  Augusto Manuel Tentori ,  Rajiv Bharadwaj ,  David Maurice Cox ,  Jennifer Chew ,  Joakim Lundeberg ,  Felice Alessio Bava ,  Linda Kvastad ,  Andrew D. Price

IPC: C12Q1/6844 ,  B01L3/00 ,  B01L9/00 ,  C12N15/10 ,  C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  G01N21/64 ,  G01N33/483 ,  G01N35/00 ,  G02B21/16 ,  G02B21/26 ,  G02B21/34 ,  G02B21/36

Abstract: A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

公开(公告)号：US20240408593A1

公开(公告)日：2024-12-12

申请号：US18664237

申请日：2024-05-14

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  Augusto Manuel Tentori ,  Siyuan Xing ,  Rajiv Bharadwaj ,  Bill Kengli Lin ,  Felice Alessio Bava ,  Pratomo Putra Alimsijah ,  Nabil Mikhaiel

IPC: B01L3/00 ,  C12Q1/6837 ,  C12Q1/6841 ,  G01N1/31

Abstract: Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.

公开(公告)号：US20240401118A1

公开(公告)日：2024-12-05

申请号：US18765751

申请日：2024-07-08

Applicant: 10x Genomics, Inc.

Inventor： Augusto Manuel Tentori ,  Hanyoup Kim ,  Felice Alessio Bava ,  Rajiv Bharadwaj

IPC: C12Q1/6841

Abstract: Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include poly-thymine sequence and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

公开(公告)号：US12128403B2

公开(公告)日：2024-10-29

申请号：US17873586

申请日：2022-07-26

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  Augusto Manuel Tentori ,  Siyuan Xing ,  Rajiv Bharadwaj ,  Bill Kengli Lin ,  Felice Alessio Bava ,  Pratomo Putra Alimsijah ,  Nabil Mikhaiel

IPC: B01L3/00 ,  C12Q1/6837 ,  C12Q1/6841 ,  G01N1/31

CPC classification number: B01L3/502715 ,  B01L3/502723 ,  C12Q1/6837 ,  C12Q1/6841 ,  G01N1/312 ,  B01L2200/027 ,  B01L2300/0822 ,  B01L2400/0421

Abstract: Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.

公开(公告)号：US20240219701A1

公开(公告)日：2024-07-04

申请号：US18419166

申请日：2024-01-22

Applicant: 10x Genomics, Inc.

Inventor： Augusto Manuel Tentori ,  Rajiv Bharadwaj ,  Felice Alessio Bava

IPC: G02B21/26 ,  G01N33/483 ,  G01N35/00 ,  G02B21/34 ,  G02B21/36

CPC classification number: G02B21/26 ,  G01N33/4833 ,  G01N35/00732 ,  G02B21/34 ,  G02B21/365 ,  G01N2035/00752

Abstract: A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

公开(公告)号：US12024741B2

公开(公告)日：2024-07-02

申请号：US17665169

申请日：2022-02-04

Applicant: 10x Genomics, Inc.

Inventor： Augusto Manuel Tentori ,  Rajiv Bharadwaj ,  Felice Alessio Bava

IPC: B01L9/00 ,  B01L3/00 ,  C12N15/10 ,  C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  G01N21/64 ,  G01N33/483 ,  G01N35/00 ,  G02B21/16 ,  G02B21/26 ,  G02B21/34 ,  G02B21/36 ,  C12Q1/6855 ,  C12Q1/6869

CPC classification number: C12Q1/6844 ,  B01L3/50853 ,  B01L3/545 ,  B01L9/523 ,  C12N15/1065 ,  C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  G01N21/6458 ,  G01N33/4833 ,  G01N35/00732 ,  G02B21/16 ,  G02B21/26 ,  G02B21/34 ,  G02B21/365 ,  B01L2300/0829 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2525/161 ,  C12Q2525/179 ,  C12Q2527/156 ,  C12Q2543/101 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2565/60 ,  C12Q2600/158 ,  G01N2021/6439 ,  G01N2035/00752

Abstract: A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

公开(公告)号：US11898205B2

公开(公告)日：2024-02-13

申请号：US17165342

申请日：2021-02-02

Applicant: 10x Genomics, Inc.

Inventor： Felice Alessio Bava

IPC: C12Q1/6876 ,  C12Q1/6837

CPC classification number: C12Q1/6876 ,  C12Q1/6837 ,  C12Q2521/507 ,  C12Q2525/173 ,  C12Q2531/125 ,  C12Y605/01001 ,  C12Y605/01003

Abstract: This disclosure relates to methods for increasing capture efficiency of a spatial array using rolling circle amplification of a padlock probe that hybridizes to a capture probe. Also provided are methods for using such spatial arrays to detect a biological analyte in a biological sample.