公开(公告)号：US08497356B2

公开(公告)日：2013-07-30

申请号：US13201502

申请日：2010-02-17

Applicant: Ashutosh Chilkoti ,  Weiping Gao

Inventor： Ashutosh Chilkoti ,  Weiping Gao

IPC: C07K1/00

CPC classification number: C07K1/1075 ,  A61K38/00 ,  A61K47/60 ,  C07K1/02

Abstract: Methods for producing biomolecule-polymer conjugates, such as polypeptide-polymer conjugates, include attachment of an initiator agent to a biomolecule and in situ polymerization of a polymer from defined sites on the biomolecule. The conjugates may have desirable pharmacological properties and may be used therapeutically.

Abstract translation: 用于生产生物分子 - 聚合物缀合物（例如多肽 - 聚合物共轭物）的方法包括将引发剂连接到生物分子上，并且聚合物从生物分子上的限定位点的原位聚合。 缀合物可具有期望的药理学性质并且可以在治疗上使用。

公开(公告)号：US20130157889A1

公开(公告)日：2013-06-20

申请号：US13698383

申请日：2011-05-17

Applicant: Ashutosh Chilkoti ,  Angus Hucknall

Inventor： Ashutosh Chilkoti ,  Angus Hucknall

IPC: G01N33/543

CPC classification number: G01N33/54366 ,  A61B5/1411 ,  A61B5/150022 ,  A61B5/150358 ,  A61B5/150755 ,  A61B5/15142 ,  A61B5/157 ,  A61B2562/0295 ,  G01N33/54393

Abstract: Disclosed are devices and methods for detecting analytes from a sample.

Abstract translation: 公开了用于检测来自样品的分析物的装置和方法。

公开(公告)号：US08367314B2

公开(公告)日：2013-02-05

申请号：US12644197

申请日：2009-12-22

Applicant: Ashutosh Chilkoti

Inventor： Ashutosh Chilkoti

IPC: C12Q1/00

CPC classification number: C12Q1/682 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q1/6837 ,  C40B50/18 ,  G01N33/5302 ,  G01N33/54353 ,  G01N33/54393 ,  C12Q2525/313 ,  C12Q2521/131

Abstract: An article such as a biosensor having a nonfouling surface thereon is described. The article comprises: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; (c) a polymer layer comprising brush molecules formed on the linking layer; and (d) optionally but preferably, a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) coupled to the brush molecules. The polymer layer is preferably formed by the process of surface-initiated polymerization (SIP) of monomeric units thereon. Preferably, each of the monomeric units comprises a monomer (for example, a vinyl monomer) core group having at least one protein-resistant head group coupled thereto, to thereby form the brush molecule on the surface portion. Methods of using the articles are also described.

Abstract translation: 描述了诸如其上具有非结垢表面的生物传感器的物品。 该制品包括：（a）具有表面部分的基底; （b）表面部分上的连接层; （c）包含在所述连接层上形成的刷分子的聚合物层; 和（d）任选但优选地，偶联到刷分子的特异性结合对（例如蛋白质，肽，抗体，核酸等）的第一成员。 聚合物层优选通过其上单体单元的表面引发聚合（SIP）的方法形成。 优选地，每个单体单元包含具有至少一个与其结合的蛋白质抗性头基团的单体（例如，乙烯基单体）核心基团，从而在表面部分上形成刷分子。 还描述了使用制品的方法。

公开(公告)号：US20110236384A1

公开(公告)日：2011-09-29

申请号：US13012475

申请日：2011-01-24

Applicant: Lori A. SETTON ,  Ashutosh Chilkoti ,  Virginia B. Kraus ,  Helawe Betre ,  Matthew R. Dreher

Inventor： Lori A. SETTON ,  Ashutosh Chilkoti ,  Virginia B. Kraus ,  Helawe Betre ,  Matthew R. Dreher

IPC: A61K38/16 ,  A61K39/395 ,  A61P19/02

CPC classification number: C07K14/78 ,  A61K9/0024 ,  A61K47/42 ,  A61K47/642 ,  A61K47/6435 ,  C07K14/7155

Abstract: A method for delivering a drug depot of a compound of interest to a selected region in a subject. The method comprises administering a composition directly to said region of interest, the composition comprising the compound of interest to be delivered (such as an antiinflammatory agent or a chemotherapeutic agent) and a polymer (such as an elastin-like peptide or ELP) that undergoes an inverse temperature phase transition, so that a sustained release of the compound of interest at the selected region is provided. Compositions useful for carrying out the invention are also described.

Abstract translation: 将感兴趣的化合物的药物贮库递送至受试者的选定区域的方法。 该方法包括将组合物直接施用于所述感兴趣的区域，所述组合物包含待递送的目标化合物（例如抗炎剂或化学治疗剂）和经历的聚合物（例如弹性蛋白样肽或ELP） 逆温度相变，从而提供在所选择的区域的感兴趣的化合物的持续释放。 还描述了用于实施本发明的组合物。

公开(公告)号：US20090247424A1

公开(公告)日：2009-10-01

申请号：US12405300

申请日：2009-03-17

Applicant: Ashutosh Chilkoti ,  Angus Hucknall

Inventor： Ashutosh Chilkoti ,  Angus Hucknall

IPC: C40B30/04 ,  C40B40/08 ,  C40B40/10 ,  C40B50/18

CPC classification number: G01N33/54366 ,  G01N33/54353

Abstract: An article such as a biomolecular detector or biosensor having a nonfouling surface thereon includes:(a) a substrate having a surface portion; (b) a linking layer on the surface portion; and (c) a polymer layer formed on the linking layer; and (d) a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) bound to the polymer layer. Methods of making and using the articles are also described.

Abstract translation: 诸如生物分子检测器或其上具有非结垢表面的生物传感器的物品包括：（a）具有表面部分的基底; （b）表面部分上的连接层; 和（c）形成在所述连接层上的聚合物层; 和（d）与聚合物层结合的特异性结合对（例如，蛋白质，肽，抗体，核酸等）的第一成员。 还描述了制造和使用制品的方法。

公开(公告)号：US20070087114A1

公开(公告)日：2007-04-19

申请号：US11583232

申请日：2006-10-19

Applicant: Ashutosh Chilkoti ,  Zhongping Yang ,  Jinho Hyun

Inventor： Ashutosh Chilkoti ,  Zhongping Yang ,  Jinho Hyun

IPC: A61L33/00

CPC classification number: B82Y30/00 ,  A61L27/34 ,  A61L27/50 ,  A61L27/54 ,  A61L2300/22 ,  A61L2300/23 ,  A61L2300/252 ,  A61L2300/258 ,  A61L2300/604 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B05D1/185 ,  B05D1/283 ,  B82Y40/00 ,  C07B2200/11 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/545 ,  Y10T156/19

Abstract: Methods of attaching a ligand to a surface are described that include contacting a surface with a substrate containing an amphiphilic comb polymer. The substrate is configured to provide a pattern of the amphiphilic comb polymer on a selected region of the surface. The substrate can be separated from the surface leaving the amphiphilic comb polymer on the selected region of the surface, thus providing a selected region of the surface having amphiphilic comb polymer on it. A ligand can then be deposited on the surface such that the selected region of the surface having the amphiphilic comb polymer is substantially free of the ligand.

公开(公告)号：US07163712B2

公开(公告)日：2007-01-16

申请号：US10176366

申请日：2002-06-20

Applicant: Ashutosh Chilkoti ,  Zhongping Yang ,  Jinho Hyun

Inventor： Ashutosh Chilkoti ,  Zhongping Yang ,  Jinho Hyun

IPC: B05D5/00

CPC classification number: B82Y30/00 ,  A61L27/34 ,  A61L27/50 ,  A61L27/54 ,  A61L2300/22 ,  A61L2300/23 ,  A61L2300/252 ,  A61L2300/258 ,  A61L2300/604 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B05D1/185 ,  B05D1/283 ,  B82Y40/00 ,  C07B2200/11 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/545 ,  Y10T156/19

Abstract: Methods of attaching a ligand to a surface are described that include contacting a surface with a substrate containing an amphiphilic comb polymer. The substrate is configured to provide a pattern of the amphiphilic comb polymer on a selected region of the surface. The substrate can be separated from the surface leaving the amphiphilic comb polymer on the selected region of the surface, thus providing a selected region of the surface having amphiphilic comb polymer on it. A ligand can then be deposited on the surface such that the selected region of the surface having the amphiphilic comb polymer is substantially free of the ligand.

Abstract translation: 描述了将配体连接到表面的方法，其包括使表面与含有两亲性梳状聚合物的基材接触。 衬底被配置为在表面的选定区域上提供两亲梳状聚合物的图案。 衬底可以从离开表面的选定区域的两亲性梳状聚合物的表面分离，从而在其上提供具有两亲性梳状聚合物的表面的选定区域。 然后可以将配体沉积在表面上，使得具有两亲梳状聚合物的表面的选定区域基本上不含配体。

公开(公告)号：US06755621B2

公开(公告)日：2004-06-29

申请号：US10459483

申请日：2003-06-12

Applicant: Gabriel Lopez ,  Ashutosh Chilkoti ,  Plamen Atanassov ,  Venkata R. Goparaju

Inventor： Gabriel Lopez ,  Ashutosh Chilkoti ,  Plamen Atanassov ,  Venkata R. Goparaju

IPC: F04B4900

CPC classification number: F16K99/0001 ,  B01D15/3861 ,  B01D15/3876 ,  B82Y15/00 ,  F15C5/00 ,  F16K99/0025 ,  F16K99/0036 ,  F16K99/0044 ,  F16K2099/0076 ,  F16K2099/0084 ,  F16K2099/0086 ,  Y10T137/0324 ,  Y10T137/7737 ,  Y10T137/87265

Abstract: The present invention provides a hybrid material and a method for forming a hybrid material comprising actuator made from a stimuli responsive polymer mounted in a porous framework. The present invention also provides devices employing the actuator of the present invention.

Abstract translation: 本发明提供一种混合材料和用于形成混合材料的方法，所述混合材料包括由安装在多孔框架中的刺激响应聚合物制成的致动器。 本发明还提供了采用本发明的致动器的装置。

公开(公告)号：US06491061B1

公开(公告)日：2002-12-10

申请号：US09790974

申请日：2001-02-23

Applicant: Gabriel Lopez ,  Ashutosh Chilkoti ,  Plamen Atanassov ,  Venkata Goparaju

Inventor： Gabriel Lopez ,  Ashutosh Chilkoti ,  Plamen Atanassov ,  Venkata Goparaju

IPC: F16K1102

CPC classification number: F16K99/0001 ,  B01D15/3861 ,  B01D15/3876 ,  B82Y15/00 ,  F15C5/00 ,  F16K99/0025 ,  F16K99/0036 ,  F16K99/0044 ,  F16K2099/0076 ,  F16K2099/0084 ,  F16K2099/0086 ,  Y10T137/0324 ,  Y10T137/7737 ,  Y10T137/87265

Abstract: The present invention provides a hybrid material and a method for forming a hybrid material comprising actuator made from a stimuli responsive polymer mounted in a porous framework. The present invention also provides devices employing the actuator of the present invention.

Abstract translation: 本发明提供一种混合材料和用于形成混合材料的方法，所述混合材料包括由安装在多孔框架中的刺激响应聚合物制成的致动器。 本发明还提供了采用本发明的致动器的装置。

公开(公告)号：US08470967B2

公开(公告)日：2013-06-25

申请号：US13245459

申请日：2011-09-26

Applicant: Ashutosh Chilkoti ,  Felipe Garcia Quiroz ,  Miriam Amiram

Inventor： Ashutosh Chilkoti ,  Felipe Garcia Quiroz ,  Miriam Amiram

IPC: A61K9/14 ,  A61K47/42 ,  A61K8/64 ,  A61K38/00 ,  A61K8/11

CPC classification number: C07K14/001 ,  A61K8/11 ,  A61K8/64 ,  A61K9/5123 ,  A61K9/5169 ,  A61K38/00 ,  A61K47/42 ,  B82Y5/00 ,  C07K14/78 ,  C07K19/00 ,  C07K2319/00 ,  C07K2319/21

Abstract: The present disclosure describes environmentally responsive polypeptides capable of displaying stimuli-triggered conformational changes in a reversible or irreversible manner that may be accompanied by aggregation. Polypeptides include a number of repeated motifs and may be elastomeric or non-elastomeric. The polypeptides may be used to deliver therapeutics to a biological site and to develop bioactive polypeptides that are environmentally responsive.

Abstract translation: 本公开描述了能够以可能伴随聚集的可逆或不可逆方式显示刺激触发的构象变化的环境敏感多肽。 多肽包括许多重复基序，并且可以是弹性体或非弹性体。 多肽可用于将治疗剂递送至生物部位并开发环境反应性的生物活性多肽。