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    Cellular arrays comprising encoded cells
    33.
    发明申请
    Cellular arrays comprising encoded cells 审中-公开
    包含编码细胞的细胞阵列

    公开(公告)号：US20050158702A1

    公开(公告)日：2005-07-21

    申请号：US10363240

    申请日：2001-09-05

    Applicant: John Stuelpnagel ,  Mark Chee

    Inventor： John Stuelpnagel ,  Mark Chee

    IPC: C07B61/00 ,  G01N33/50 ,  G01N33/543 ,  C12Q1/00 ,  C12M1/34 ,  G01N33/53 ,  G01N33/567

    CPC classification number: G01N33/502 ,  C40B40/00 ,  G01N33/5008 ,  G01N33/54373

    Abstract: A biosensor, sensor array, sensing method and sensing apparatus are provided in which individual cells or randomly mixed populations of cells, having unique response characteristics to chemical and biological materials, are deployed in a plurality of discrete sites on a substrate. In a preferred embodiment, the discrete sites comprise microwells formed at the distal end of individual fibers within a fiber optic array. The biosensor array utilizes an optically interrogatable encoding scheme for determining the identity and location of each cell type in the array and provides for simultaneous measurements of large number of individual cell respnses to target analyses. The sensing method utilizes the unique ability of cell populations to respond to biologically significant compounds in a characteristic and detectable manner. The biosensor array and measurement method may be employed in the study of biologically active materials in situ environmental monitoring, monitoring of a variety of bioprocesses, and for high throughput screening of large combinatorial chemical libraries.

    Abstract translation: 提供了生物传感器，传感器阵列，感测方法和感测装置，其中对化学和生物材料具有独特响应特性的单个细胞或随机混合的细胞群体部署在基底上的多个离散位置。 在优选实施例中，离散位置包括在光纤阵列内的各个纤维的远端处形成的微孔。 生物传感器阵列利用光学可询问编码方案来确定阵列中每种细胞类型的身份和位置，并提供大量单个细胞反应同时测量以进行目标分析。 感测方法利用细胞群体以特征和可检测方式对生物学重要化合物的独特能力。 生物传感器阵列和测量方法可用于研究生物活性材料的原位环境监测，各种生物过程的监测以及大量组合化学文库的高通量筛选。

    Method to detect gene polymorphisms and monitor allelic expression employing a probe array
    34.
    发明授权
    Method to detect gene polymorphisms and monitor allelic expression employing a probe array 有权
    使用探针阵列检测基因多态性和监测等位基因表达的方法

    公开(公告)号：US06368799B1

    公开(公告)日：2002-04-09

    申请号：US09445734

    申请日：2000-03-14

    Applicant: Mark Chee

    Inventor： Mark Chee

    IPC: C12Q168

    CPC classification number: C12Q1/6837 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y10S435/911 ,  C12Q2535/131

    Abstract: The invention provides methods of monitoring expression levels of different polymorphic forms of a gene. Such methods entail analyzing genomic DNA from an individual to determine the presence of heterozygous polymorphic forms at a polymorphic site within a transcribed sequence of a gene of interest. RNA from a tissue of the individual in which the gene is expressed is then analyzed to determine relative proportions of polymorphic forms in transcript of the gene. Having identified alleles of a gene that are expressed at different levels, the alleles can be further analyzed to locate a second polymorphism that has a causative role in the different expression levels. The methods are amenable to analyzing large collections of genes simultaneously using arrays of immobilized probes.

    Abstract translation: 本发明提供了监测基因不同多态形式的表达水平的方法。 这样的方法需要分析来自个体的基因组DNA，以确定在感兴趣的基因的转录序列内的多态性位点处存在杂合多态性形式。 然后分析来自表达基因的个体的组织的RNA，以确定基因转录本中多态形式的相对比例。 鉴定了在不同水平表达的基因的等位基因，可以进一步分析等位基因以定位在不同表达水平中起决定作用的第二多态性。 该方法适用于使用固定化探针阵列同时分析大量基因。

    Iterative resequencing
    40.
    发明授权
    Iterative resequencing 失效
    迭代重排序

    公开(公告)号：US07144699B2

    公开(公告)日：2006-12-05

    申请号：US09381480

    申请日：1998-03-19

    Applicant: Mark Chee

    Inventor： Mark Chee

    IPC: C12Q1/68 ,  C12M1/36 ,  C07H21/04

    CPC classification number: C12Q1/6874

    Abstract: The invention provides iterative methods of analyzing a target nucleic acid that represents a variant of a reference nucleic acid. An array of probes is designed to be complementary to an estimated sequence of a target nucleic acid. The array of probes is then hybridized to the target nucleic acid. The target sequence is reestimated from hybridization pattern of the array to the target nucleic acid. A further array of probes is then designed to be complementary to the reestimated sequence, and this array is used to obtain a further reestimate of the sequence of the target nucleic acid. By performing iterative cycles of array design and target sequence estimation, the estimated sequence of the target converges with the true sequence.

    Abstract translation: 本发明提供了分析代表参考核酸的变体的靶核酸的迭代方法。 探针阵列设计成与靶核酸的估计序列互补。 然后将探针阵列与目标核酸杂交。 靶序列从阵列的杂交模式重新估计为靶核酸。 然后将另外的探针阵列设计为与重新估计的序列互补，并且该阵列用于获得靶核酸序列的进一步重新估计。 通过执行阵列设计和靶序列估计的迭代循环，目标的估计序列与真实序列收敛。

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