USING CELL DEBRIS GENERATED FROM PHA RECOVERY FOR ENHANCED CELL GROWTH AND BIOPOLYESTER FORMATION
    31.
    发明申请
    USING CELL DEBRIS GENERATED FROM PHA RECOVERY FOR ENHANCED CELL GROWTH AND BIOPOLYESTER FORMATION 审中-公开
    使用从PHA恢复产生的细胞凋亡增强细胞生长和生物聚合物形成

    公开(公告)号:US20120252081A1

    公开(公告)日:2012-10-04

    申请号:US13502323

    申请日:2009-10-16

    申请人: Jian Yu

    发明人: Jian Yu

    IPC分类号: C12P7/62

    CPC分类号: C12P7/625

    摘要: The present invention relates to a process for producing biodegradable polymeric materials including polyhydroxyalkanoates (PHAs) by using the cell debris left from PHA recovery and purification. The process comprises: (a) cultivating PHA-producing microbial cells in a medium solution containing an organic carbon source to form PHAs that are accumulated in the cells as inclusion bodies; (b) harvesting the cells from the spent medium and solubilizing the non-PHA cell mass to obtain a PHA solid and a cell debris solution; (c) separating the PHA solid from the cell debris solution; (d) feeding the cell debris solution to the cultivation step (a). By reusing the cell debris generated from PHA recovery, the invention avoids disposal of a large amount of aqueous waste. In addition, a remarkable increase of cell growth and PHA synthesis is achieved, because the cell debris can be readily assimilated by the microbial cells as the nutrients.

    摘要翻译: 本发明涉及通过使用从PHA回收和纯化留下的细胞碎片来生产包括聚羟基链烷酸酯(PHA)的生物可降解聚合物材料的方法。 该方法包括:(a)在含有机碳源的培养基溶液中培养产生PHA的微生物细胞,形成作为包涵体积聚在细胞中的PHA; (b)从废培养基中收获细胞并溶解非PHA细胞块以获得PHA固体和细胞碎片溶液; (c)将PHA固体与细胞碎片溶液分离; (d)将细胞碎片溶液送入培养步骤(a)。 通过重新利用由PHA回收产生的细胞碎片,本发明避免了大量的含水废物的处置。 此外,实现了细胞生长和PHA合成的显着增加,因为细胞碎片可以容易地被微生物细胞作为营养物质吸收。

    Method of Determining Surface Pore Mouth Diameter Distribution of Porous Material
    33.
    发明申请
    Method of Determining Surface Pore Mouth Diameter Distribution of Porous Material 有权
    确定多孔材料表面孔径分布的方法

    公开(公告)号:US20110167897A1

    公开(公告)日:2011-07-14

    申请号:US13119722

    申请日:2009-11-03

    申请人: Yan Huang Jian Yu

    发明人: Yan Huang Jian Yu

    IPC分类号: G01N15/08

    CPC分类号: G01N15/088

    摘要: A method of determining surface pore diameter distribution of a porous material is provided. A compressed gas is charged from backside of a sample of a porous body, a surface of the sample is immersed into a liquid wetting agent, and a gas pressure is regulated from high to low, such that pore mouths of the surface of the porous body are blocked by the wetting agent in an order from small to large pore sizes, and a gas flow rate is more rapidly decreased. When the pressure is decreased to a value, the gas flow rate is reduced to zero and the pressure at this time is corresponding to the largest pore mouth size of the surface of the porous body. The pore size distribution is determined by comparing the obtained gas pressure—flow rate curve (wet curve) with a gas pressure—flow rate curve (dry curve) obtained for the sample under a dry state. The pore size and the pore distribution are calculated based on the conventional bubble pressure method. The measurement of the surface pore distribution of the porous body is of great significance for investigating the support performance of the porous body.

    摘要翻译: 提供了确定多孔材料的表面孔径分布的方法。 从多孔体的样品的背面填充压缩气体,将样品的表面浸入液体润湿剂中,并将气体压力从高调至低,使得多孔体的表面的孔口 被润湿剂按照从小到大的孔径的顺序阻挡,并且气体流速更快地降低。 当压力降低到一个值时,气体流量减少到零,此时的压力对应于多孔体表面的最大孔口尺寸。 通过将获得的气体压力 - 流量曲线(湿曲线)与在干燥状态下获得的样品的气体压力 - 流量曲线(干曲线)进行比较来确定孔径分布。 基于常规气泡压力法计算孔径和孔分布。 多孔体的表面孔分布的测量对于研究多孔体的支撑性能具有重要意义。

    Recovery and purification of polyhydroxyalkanoates
    34.
    发明授权
    Recovery and purification of polyhydroxyalkanoates 有权
    聚羟基链烷酸酯的回收和纯化

    公开(公告)号:US07514525B2

    公开(公告)日:2009-04-07

    申请号:US11684110

    申请日:2007-03-09

    申请人: Jian Yu

    发明人: Jian Yu

    IPC分类号: C08G64/00 C08G63/02

    CPC分类号: C08G63/90 C08G63/06

    摘要: The present invention relates to a method to recover, purify and isolate polyhydroxyalkanoate (PHA) biopolymers from PHA-containing cell mass, which includes: (a) solubilizing the non-PHA cell mass in an acidic solution, leaving a suspension of partially crystallized PHA granules; (b) adjusting the pH of the suspension to 7-11 and separating the PHA solids from the dissolved non-PHA cellular mass; (c) re-suspending the PHA solids in a bleaching solution for decolorization; and (d) drying the resulting PHA solids. About 95% or greater of original PHA in cell mass is recovered, and the purity of PHA solids is about 97% or above. The weight average molecular mass of the purified biopolyesters is about 500 kDa or greater.

    摘要翻译: 本发明涉及从含PHA的细胞团中回收,纯化和分离聚羟基链烷酸酯(PHA)生物聚合物的方法,其包括:(a)将非PHA细胞块溶解在酸性溶液中,留下部分结晶的PHA的悬浮液 颗粒; (b)将悬浮液的pH调节至7-11,并将PHA固体与溶解的非PHA细胞团分离; (c)将PHA固体重新悬浮在漂白溶液中以进行脱色; 和(d)干燥所得的PHA固体。 回收大约95%以上的原始PHA,PHA固体纯度约97%以上。 纯化的生物聚酯的重均分子量为约500kDa或更大。

    Production of submicron diameter fibers by two-fluid electrospinning process
    35.
    发明申请
    Production of submicron diameter fibers by two-fluid electrospinning process 审中-公开
    通过双液静电纺丝工艺生产亚微米直径的纤维

    公开(公告)号:US20060213829A1

    公开(公告)日:2006-09-28

    申请号:US11089931

    申请日:2005-03-25

    IPC分类号: B01D24/00 B01D39/00

    摘要: Electrospinning of materials that are difficult or impossible to process into nanofibers by conventional fiber-forming techniques or by electrospinning are prepared by an electrospinning procedure which uses an electrospinnable outer “shell” fluid around an inner “core” fluid, which may or may not be electrospinnable, to form nanofibers of the inner core fluid having a core/shell morphology. The resulting shell around the nanofiber can remain in place or be removed during post-processing with the core of the fiber remaining intact. The dual-fluid electrospinning process can produce core fibers having diameters less than 100 nm, insulated nanowires, as well as tough, bio-compatible silk fibers. Alternatively, the core can be removed leaving a hollow fiber of the shell fluid.

    摘要翻译: 通过常规纤维形成技术或静电纺丝将难以或不可能加工成纳米纤维的材料的静电纺丝通过静电纺丝方法制备,所述静电纺丝方法使用围绕内部“芯”流体的可电纺的外部“壳”流体,其可以是或可以不是 电纺丝,形成具有核/壳形态的内芯流体的纳米纤维。 纳米纤维周围的所得壳可以保留在适当位置,或者在后处理期间被除去,纤维的芯保持完整。 双流体静电纺丝工艺可以生产直径小于100nm的芯纤维,绝缘纳米线以及坚韧的生物相容的丝纤维。 或者,可以去除芯体,留下壳体流体的中空纤维。

    Apparatus and method for microcontroller debugging
    36.
    发明授权
    Apparatus and method for microcontroller debugging 有权
    微控制器调试的装置和方法

    公开(公告)号:US06915416B2

    公开(公告)日:2005-07-05

    申请号:US09752567

    申请日:2000-12-28

    IPC分类号: G06F11/36 G06F13/24

    CPC分类号: G06F11/3644

    摘要: Apparatus and method for microcontroller debugging. A preferred embodiment microcontroller integrated circuit comprises debug circuitry on the integrated circuit. The debug circuitry is capable of breaking normal instruction execution based on an address breakpoint, a stack pointer breakpoint, or a single step breakpoint. Upon detection of a valid breakpoint, the debug circuitry substitutes a jump to a debug program instruction in place of the next normal application program instruction. The debug program then may provide microcontroller status to a developer, allowing the developer to debug the application program. Upon completion of the debug program, control of the microcontroller is returned to the application program at the point of interruption.

    摘要翻译: 微控制器调试的装置和方法 优选实施例微控制器集成电路包括集成电路上的调试电路。 调试电路能够基于地址断点,堆栈指针断点或单个步骤断点来中断正常指令执行。 在检测到有效的断点时,调试电路代替下一个正常应用程序指令的跳转代替调试程序指令。 然后,调试程序可以向开发人员提供微控制器状态,允许开发人员调试应用程序。 完成调试程序后,微控制器的控制在中断点返回到应用程序。

    Methods and apparatus for delivering application data to applications
    37.
    发明授权
    Methods and apparatus for delivering application data to applications 有权
    将应用程序数据传送到应用程序的方法和设备

    公开(公告)号:US09210476B1

    公开(公告)日:2015-12-08

    申请号:US13485822

    申请日:2012-05-31

    IPC分类号: H04N21/488 H04N7/16

    摘要: Methods and apparatus for supplying unbound applications with data via in-band program channel signaling are described. Unbound applications are STB or other customer premise device applications which continue to run when a tuner in the device changes between program channels. Applications executed in a STB, in accordance with the present invention, e.g., eBIF applications, continue to run despite changes in program channels with data being supplied via in-band signaling, out of band signaling, or a combination of in-band and out of band signaling. The methods and apparatus are well suited for supporting delivery of program catalog information relevant to an individual program channel using in-band signaling reducing or eliminating the need to delivery a multi-channel program catalog via out of band signaling to support various VOD applications.

    摘要翻译: 描述了通过带内节目信道信令向未绑定应用提供数据的方法和装置。 未绑定的应用程序是STB或其他客户端设备应用程序,当设备中的调谐器在程序通道之间变化时,继续运行。 根据本发明,例如eBIF应用程序在STB中执行的应用程序继续运行,尽管节目信道的改变,其中数据通过带内信令,带外信令或带内和外部的组合提供 的频带信令。 所述方法和装置非常适合于支持使用带内信令传送与单个节目信道相关的节目目录信息,从而减少或消除了通过带外信令发送多频道节目目录以支持各种VOD应用的需要。

    Programmable fuse structure and methods of forming
    38.
    发明授权
    Programmable fuse structure and methods of forming 有权
    可编程熔丝结构和成型方法

    公开(公告)号:US08981523B2

    公开(公告)日:2015-03-17

    申请号:US13419877

    申请日:2012-03-14

    摘要: Methods of forming an electrically programmable fuse (e-fuse) structure and the e-fuse structure are disclosed. Various embodiments of forming the e-fuse structure include: forming a dummy poly gate structure to contact a surface of a silicon structure, the dummy poly gate structure extending only a part of a length of the silicon structure; and converting an unobstructed portion of the surface of the silicon structure to silicide to form a thinned strip of the silicide between two end regions.

    摘要翻译: 公开了形成电可编程熔丝(e熔丝)结构和电熔体结构的方法。 形成e熔丝结构的各种实施例包括:形成虚拟多晶硅结构以接触硅结构的表面,所述虚设多晶硅结构仅延伸所述硅结构的长度的一部分; 以及将所述硅结构的表面的无障碍部分转化为硅化物以在两个端部区域之间形成所述硅化物的薄化带。

    MEDIA SYNCHRONIZATION WITHIN HOME NETWORK USING SET-TOP BOX AS GATEWAY
    39.
    发明申请
    MEDIA SYNCHRONIZATION WITHIN HOME NETWORK USING SET-TOP BOX AS GATEWAY 有权
    在家庭网络中使用机顶盒作为网关的媒体同步化

    公开(公告)号:US20140351860A1

    公开(公告)日:2014-11-27

    申请号:US14448121

    申请日:2014-07-31

    摘要: Techniques disclosed herein include systems and methods for transmitting TV program related content to IP connected devices on a home network, or other local area network. Users can watch television while simultaneously receiving content related to TV programs currently being displayed on a television. TV centric and TV program related content can be transmitted to IP connected devices. Moreover, this TV program related content can be displayed at the same time as a television program is being displayed on a television. As such, the system can provide media synchronization within home network using the set-top box as a gateway for delivering the content. Users can watch TV at home, and at the same time receive TV program related content on a laptop, tablet computer, mobile phone, etc.

    摘要翻译: 本文公开的技术包括用于向家庭网络或其他局域网上的IP连接设备发送TV节目相关内容的系统和方法。 用户可以同时收看与电视上正在显示的电视节目有关的内容。 电视中心和电视节目相关内容可以传输到IP连接的设备。 此外,与电视节目正在显示在电视机上的同时,可以显示该TV节目相关的内容。 因此,系统可以使用机顶盒作为用于递送内容的网关来在家庭网络内提供媒体同步。 用户可以在家中看电视,同时在笔记本电脑,平板电脑,手机等上接收电视节目相关的内容。

    MEDIA SYNCHRONIZATION WITHIN HOME NETWORK USING SET-TOP BOX AS GATEWAY
    40.
    发明申请
    MEDIA SYNCHRONIZATION WITHIN HOME NETWORK USING SET-TOP BOX AS GATEWAY 有权
    在家庭网络中使用机顶盒作为网关的媒体同步化

    公开(公告)号:US20130305292A1

    公开(公告)日:2013-11-14

    申请号:US13468451

    申请日:2012-05-10

    IPC分类号: H04N21/643 H04N21/462

    摘要: Techniques disclosed herein include systems and methods for transmitting TV program related content to IP connected devices on a home network, or other local area network. Users can watch television while simultaneously receiving content related to TV programs currently being displayed on a television. TV centric and TV program related content can be transmitted to IP connected devices. Moreover, this TV program related content can be displayed at the same time as a television program is being displayed on a television. As such, the system can provide media synchronization within home network using the set-top box as a gateway for delivering the content. Users can watch TV at home, and at the same time receive TV program related content on a laptop, tablet computer, mobile phone, etc.

    摘要翻译: 本文公开的技术包括用于向家庭网络或其他局域网上的IP连接设备发送TV节目相关内容的系统和方法。 用户可以同时收看与电视上正在显示的电视节目有关的内容。 电视中心和电视节目相关内容可以传输到IP连接的设备。 此外,与电视节目正在显示在电视机上的同时,可以显示该TV节目相关的内容。 因此,系统可以使用机顶盒作为用于递送内容的网关来在家庭网络内提供媒体同步。 用户可以在家中看电视,同时在笔记本电脑,平板电脑,手机等上接收电视节目相关的内容。