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121 条记录 (耗时 0.056 秒)

    Droplet microactuator system
    33.
    发明授权
    Droplet microactuator system 有权
    液滴微致动器系统

    公开(公告)号：US07815871B2

    公开(公告)日：2010-10-19

    申请号：US11639664

    申请日：2006-12-15

    申请人： Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen E. Eckhardt ,  Philip Y. Paik

    发明人： Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen E. Eckhardt ,  Philip Y. Paik

    IPC分类号： B01J8/00 ,  G01N27/26

    CPC分类号： B41J2/125 ,  B01L3/502792 ,  B01L2200/143 ,  B01L2200/147 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0487 ,  B41J2/17509 ,  G01N15/1484 ,  G01N2015/1006

    摘要： The present invention relates to a droplet microactuator system. According to one embodiment, the droplet microactuator system includes: (a) a droplet microactuator configured to conduct droplet operations; (b) a magnetic field source arranged to immobilize magnetically responsive beads in a droplet during droplet operations; (c) a sensor configured in a sensing relationship with the droplet microactuator, such that the sensor is capable of sensing a signal from and/or a property of one or more droplets on the droplet microactuator; and (d) one or more processors electronically coupled to the droplet microactuator and programmed to control electrowetting-mediated droplet operations on the droplet actuator and process electronic signals from the sensor.

    摘要翻译： 液滴微致动器系统技术领域本发明涉及液滴微致动器系统。 根据一个实施例，液滴微致动器系统包括：（a）构造成进行液滴操作的液滴微致动器; （b）磁场源，布置成在液滴操作期间将磁响应珠固定在液滴中; （c）配置成与液滴微致动器的感测关系的传感器，使得传感器能够感测来自液滴微致动器上的一个或多个液滴的信号和/或特性; 和（d）电子耦合到液滴微致动器的一个或多个处理器，并被编程为控制液滴致动器上的电润湿介导的液滴操作并处理来自传感器的电子信号。

    Droplet actuator devices and methods for immunoassays and washing
    38.
    发明授权
    Droplet actuator devices and methods for immunoassays and washing 有权
    滴液致动器装置和免疫测定和洗涤方法

    公开(公告)号：US08685754B2

    公开(公告)日：2014-04-01

    申请号：US12393534

    申请日：2009-02-26

    申请人： Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Richard B. Fair

    发明人： Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Richard B. Fair

    IPC分类号： G01N33/553

    CPC分类号： G01N33/54333

    摘要： Droplet actuator devices and methods for immunoassays and washing are provided. According to one embodiment, a method of providing a droplet in contact with a surface of a super paramagnetic bead with a reduced concentration of a substance is provided and includes: (a) providing a super paramagnetic bead in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets. The set of droplets includes: (i) a droplet in contact with the super paramagnetic bead having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the super paramagnetic bead.

    摘要翻译： 提供液滴致动器装置和免疫测定和洗涤方法。 根据一个实施方案，提供了一种提供与物质浓度降低的超顺磁珠的表面接触的液滴的方法，包括：（a）提供与包含起始浓度的液滴接触的超顺磁珠 并开始物质的起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合液滴以产生一组液滴。 一组液滴包括：（i）与超顺磁珠接触的液滴，其物质相对于起始浓度降低; 和（ii）从超顺磁珠分离的液滴。

    Bead Manipulation Techniques
    40.
    发明申请
    Bead Manipulation Techniques 有权
    珠操作技术

    公开(公告)号：US20110114490A1

    公开(公告)日：2011-05-19

    申请号：US12985409

    申请日：2011-01-06

    申请人： Vamsee K. Pamula ,  Arjun Sudarsan ,  Ramakrishna Sista

    发明人： Vamsee K. Pamula ,  Arjun Sudarsan ,  Ramakrishna Sista

    IPC分类号： B01J8/08

    CPC分类号： B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/18 ,  G01N2035/1046

    摘要： The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include providing a droplet including magnetically responsive beads. The droplet may be provided within a region of a magnetic field having sufficient strength to attract the magnetically responsive beads to an edge of the droplet or towards an edge of the droplet, or otherwise regionalize or aggregate beads within the droplet. The method may also include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

    摘要翻译： 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括提供包括磁响应珠的液滴。 液滴可以设置在具有足够强度的磁场的区域内，以将磁响应珠吸引到液滴的边缘或朝向液滴的边缘，或以其他方式区分或聚集液滴内的珠粒。 该方法还可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。