PROCESS FOR THE PURIFICATION OF GLYCOPROTEINS
    31.
    发明申请
    PROCESS FOR THE PURIFICATION OF GLYCOPROTEINS 有权
    甘氨酸的纯化方法

    公开(公告)号:US20120252069A1

    公开(公告)日:2012-10-04

    申请号:US13511285

    申请日:2010-11-24

    IPC分类号: C07K1/20 C12P21/00 C07K1/36

    CPC分类号: C07K1/36 C07K14/59

    摘要: The present invention relates to a process for the purification of a glycoprotein comprising subjecting a liquid containing said glycoprotein to the steps of: a) reverse phase chromatography, b) size exclusion chromatography, and c) hydrophobic interaction chromatography. Also provided is a manufacturing process for producing a glycoprotein of interest.

    摘要翻译: 本发明涉及一种用于纯化糖蛋白的方法,包括使含有所述糖蛋白的液体经历以下步骤:a)反相色谱法,b)尺寸排阻色谱法,和c)疏水相互作用色谱法。 还提供了用于生产目的糖蛋白的制备方法。

    MICROORGANISMS CARRYING A TUMOR ANTIGEN
    36.
    发明申请
    MICROORGANISMS CARRYING A TUMOR ANTIGEN 有权
    携带肿瘤抗原的微生物

    公开(公告)号:US20140302093A1

    公开(公告)日:2014-10-09

    申请号:US14239711

    申请日:2012-08-22

    IPC分类号: A61K39/02

    摘要: The present invention pertains to microorganisms of the species Bacteroides xylanisolvens expressing a core-1 antigen on their surface. These microorganisms are in particular useful for the prophylaxis and treatment of core-1 positive diseases and are particularly characterized by a stable, homogenous high core-1 expression. Also provided are pharmaceutical compositions which comprise respective core-1 positive microorganisms or fractions thereof.

    摘要翻译: 本发明涉及在其表面表达核心-1抗原的拟杆菌木聚糖异构酶的微生物。 这些微生物特别可用于预防和治疗核心-1阳性疾病,特别是特征在于稳定的,均匀的高核心-1表达。 还提供了包含各自的核心-1阳性微生物或其级分的药物组合物。

    Process for the purification of glycoproteins
    37.
    发明授权
    Process for the purification of glycoproteins 有权
    糖蛋白纯化方法

    公开(公告)号:US08846344B2

    公开(公告)日:2014-09-30

    申请号:US13511285

    申请日:2010-11-24

    CPC分类号: C07K1/36 C07K14/59

    摘要: The present invention relates to a process for the purification of a glycoprotein comprising subjecting a liquid containing said glycoprotein to the steps of: a) reverse phase chromatography, b) size exclusion chromatography, and c) hydrophobic interaction chromatography. Also provided is a manufacturing process for producing a glycoprotein of interest.

    摘要翻译: 本发明涉及一种用于纯化糖蛋白的方法,包括使含有所述糖蛋白的液体经历以下步骤:a)反相色谱法,b)尺寸排阻色谱法,和c)疏水相互作用色谱法。 还提供了用于生产目的糖蛋白的制备方法。

    Fermentation process
    38.
    发明授权
    Fermentation process 有权
    发酵过程

    公开(公告)号:US08741365B2

    公开(公告)日:2014-06-03

    申请号:US12991827

    申请日:2009-05-13

    摘要: The present disclosure relates in one embodiment to a method of producing a fermented food, wherein a raw food material is combined with a starter culture for fermentation, wherein at least one microorganism of the genus Bacteroides and/or at least one microorganism having at least a cell surface structure which corresponds to a human carbohydrate structure is added. Further disclosed are processing aids and fermented foods containing respective microorganisms.

    摘要翻译: 在一个实施方案中,本公开涉及一种生产发酵食品的方法,其中将生食材料与用于发酵的起始培养物组合,其中至少一种拟杆菌属和/或至少一种微生物的微生物具有至少一种 加入对应于人碳水化合物结构的细胞表面结构。 进一步公开的是加工助剂和含有微生物的发酵食品。

    Tumour cell lines and uses thereof
    39.
    发明授权
    Tumour cell lines and uses thereof 有权
    肿瘤细胞系及其用途

    公开(公告)号:US08283161B2

    公开(公告)日:2012-10-09

    申请号:US13208253

    申请日:2011-08-11

    IPC分类号: C12N5/00 C12P21/06

    摘要: The present invention relates to a cell line selected from the group consisting of (a) a cell line denominated NM-F9 having the DSMZ accession number DSM ACC2606; (b) a cell line denominated NM-D4 having the DSMZ accession number DSM ACC2605; and subclones of (a) or (b). Additionally, the present invention provides a lysate of the cell lines or a molecule or mixture of molecules obtained from these cell lines as well as dendritic cells loaded with said lysate, co-cultivated or fused with cells from the cell lines, or a molecule or mixture of molecules obtained from these cell lines of the present invention. Moreover compositions, preferably pharmaceutical or vaccine compositions are provided which comprise the cell lines, lysate, molecules, mixture of molecules or dendritic cells of the present invention. In another aspect the present invention relates to methods for producing the aforementioned compositions. Furthermore, methods and uses for vaccination against or treatment or prevention of cancers and/or tumourous diseases are provided.

    摘要翻译: 本发明涉及选自(a)具有DSMZ登录号DSM ACC2606的称为NM-F9的细胞系的细胞系; (b)具有DSMZ登录号DSM ACC2605的称为NM-D4的细胞系; 和(a)或(b)的亚克隆。 此外,本发明提供细胞系的裂解物或从这些细胞系获得的分子或分子混合物以及负载有所述裂解物的树突状细胞,与来自细胞系的细胞共培养或融合,或分子或 从本发明的这些细胞系获得的分子的混合物。 此外,提供了包含本发明的细胞系,裂解物,分子,分子或树突状细胞的混合物的组合物,优选药物或疫苗组合物。 另一方面,本发明涉及制备上述组合物的方法。 此外,提供了针对或治疗或预防癌症和/或肿瘤疾病的疫苗接种的方法和用途。

    PERFUSION BIOREACTOR
    40.
    发明申请

    公开(公告)号:US20120100576A1

    公开(公告)日:2012-04-26

    申请号:US13382763

    申请日:2010-07-08

    摘要: The present invention pertains to a system for culturing cells comprising a culturing bag and a continuous flow centrifuge wherein the cells are continuously separated from the supernatant and are recycled into the culturing bag. Further provided are methods for culturing cells and for producing a biological substance using the device for culturing cells, and the use of a bag for culturing cells in said device or said methods. In particular, a perfusion system for culturing cells is provided wherein the wave technology for culturing cells is combined with continuous flow centrifugation for separating the medium from the cells.

    摘要翻译: 本发明涉及培养细胞的系统,所述系统包括培养袋和连续流动离心机,其中所述细胞与上清液连续分离并再循环到培养袋中。 还提供了培养细胞和使用培养细胞的装置生产生物物质的方法,以及使用用于在所述装置中培养细胞的袋或所述方法。 特别地,提供了一种用于培养细胞的灌注系统,其中用于培养细胞的波技术与连续流离心法相结合,用于将细胞与培养基分离。