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公开(公告)号：US20200011874A1
公开(公告)日：2020-01-09
申请号：US16556006
申请日：2019-08-29
Inventor： Daniel T. Chiu , Changfeng Wu , Jiangbo Yu
Abstract: The present disclosure provides encoded chromophoric polymer particles that are capable of, for example, optical and/or biomolecular encoding of analytes. The present disclosure also provides suspensions comprising a plurality of encoded chromophoric polymer particles. The present disclosure also provides methods of using the encoded chromophoric polymer particles and systems for performing multiplex analysis with encoded chromophoric polymer particles.
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公开(公告)号：US10429294B2
公开(公告)日：2019-10-01
申请号：US15563121
申请日：2016-04-01
Applicant: University of Washington
Inventor： Daniel T. Chiu , Chun-Ting Kuo , Jiangbo Yu
Abstract: Methods of optically marking and sorting adherent cells are provided. The methods include providing a plurality of adherent cells attached to a substrate, each adherent cell of the plurality of adherent cells having an optical marker. The methods also include selectively applying light energy to a subset of the plurality of adherent cells, and detaching the plurality of adherent cells from the substrate. These methods also provide the sorting of the plurality of adherent cells.
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公开(公告)号：US10421070B2
公开(公告)日：2019-09-24
申请号：US14869871
申请日：2015-09-29
Applicant: University of Washington
Inventor： Daniel T. Chiu , Dawn E. Cohen , Gavin D. M. Jeffries
Abstract: Embodiments of the present invention relate to methods and apparatuses for the discretization and manipulation of sample volumes that is simple, robust, and versatile. It is a fluidic device that partitions a sample by exploiting the interplay between fluidic forces, interfacial tension, channel geometry, and the final stability of the formed droplet and/or discretized volume. These compartmentalized volumes allow for isolation of samples and partitioning into a localized array that can subsequently be manipulated and analyzed. The isolation of the discretized volumes along with the device's inherent portability render our invention versatile for use in many areas, including but not limited to PCR, digital PCR, biological assays for diagnostics and prognostics, cancer diagnosis and prognosis, high throughput screening, single molecule and single cell reactions or assays, the study crystallization and other statistical processes, protein crystallization, drug screening, environmental testing, and the coupling to a wide range of analytical detection techniques for biomedical assays and measurements. The minimal fluid interconnects and simple flow geometry makes the device easy to use and implement, economical to fabricate and operate, and robust in its operations.
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公开(公告)号：US20190120857A1
公开(公告)日：2019-04-25
申请号：US16171918
申请日：2018-10-26
Inventor： Daniel T. Chiu , Perry G. Schiro , Jason S. Kuo
IPC: G01N33/68 , G01N33/574 , G01N33/53
Abstract: Provided herein, among other aspects, are methods and apparatuses for ranking aliquots from a suspension containing bioparticles. In certain embodiments, the bioparticles may be cells, organelles, proteins, DNAs, debris of biological origin, microbeads coated with biological compounds, or viral particles. As such, the methods and apparatuses provided herein may be used to quantify rare cells such as circulating cancer cells, fetal cells and other rare cells present in bodily fluids for disease diagnosis, prognosis, or treatment.
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公开(公告)号：US10067139B2
公开(公告)日：2018-09-04
申请号：US14373835
申请日：2013-02-01
Inventor： Daniel T. Chiu , Yuhui Jin , Fangmao Ye , Changfeng Wu , Yang-Hsiang Chan
Abstract: Polymer nanoparticles and related methods include polymer dots having a coating including a polyelectrolyte polymer. The polymer dots can have a polyelectrolyte coating that can improve colloidal stability of the particles as compared to polymer dots not having the coating. A method of preparing a population of nanoparticles. The methods can include, e.g., providing the population of nanoparticles having a condensed semiconducting polymer; and combining, in a first aqueous solution comprising polyelectrolytes, the population of nanoparticles having the condensed semiconducting polymer to form a population of nanoparticles having a polyelectrolyte coating surrounding the condensed semiconducting polymer of each of the nanoparticles in the population. The methods can include a step of forming the condensed semiconducting polymer using nanoprecipitation or miniemulsion techniques. The polyelectrolyte coating can completely surround the condensed semiconducting polymer.
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Patent Agency Ranking
公开(公告)号：US20170145490A1
公开(公告)日：2017-05-25
申请号：US15230162
申请日：2016-08-05
Inventor： Daniel T. Chiu , Bryant S. Fujimoto , Alexander R. Gansen , Gloria S. Yen , Robert M. Lorenz
IPC: C12Q1/68
CPC classification number: C12Q1/6851 , C12Q1/6806 , C12Q2527/146 , C12Q2531/113 , C12Q2545/114
Abstract: Methods, devices and systems for performing digital measurements are provided.
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公开(公告)号：US20170003293A1
公开(公告)日：2017-01-05
申请号：US15100056
申请日：2014-11-25
Inventor： Daniel T. Chiu , Changfeng Wu , Jiangbo Yu
CPC classification number: G01N33/58 , C09B23/0066 , C09B23/04 , C09B57/007 , C09B69/109 , C09K11/02 , C09K11/06 , C09K2211/1007 , C09K2211/1011 , C09K2211/1044 , C09K2211/1416 , C09K2211/182 , G01N21/6408 , G01N21/6428 , G01N33/582 , G01N33/587 , G01N33/588 , G01N2021/6417 , G01N2021/6439 , G01N2021/6441 , G01N2201/12
Abstract: The present disclosure provides encoded chromophoric polymer particles that are capable of, for example, optical and/or biomolecular encoding of analytes. The present disclosure also provides suspensions comprising a plurality of encoded chromophoric polymer particles. The present disclosure also provides methods of using the encoded chromophoric polymer particles and systems for performing multiplex analysis with encoded chromophoric polymer particles.
Abstract translation: 本公开提供了能够例如分析物的光学和/或生物分子编码的经编码的发色聚合物颗粒。 本公开还提供了包含多个编码的发色聚合物颗粒的悬浮液。 本公开还提供了使用经编码的发色聚合物颗粒和系统用编码的发色聚合物颗粒进行多重分析的方法。
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公开(公告)号：US20140302516A1
公开(公告)日：2014-10-09
申请号：US14213290
申请日：2014-03-14
Inventor： Daniel T. Chiu , Wei Sun , Jiangbo Yu , Changfeng Wu , Fangmao Ye
IPC: G01N33/569
Abstract: Lyophilized polymer dot compositions are provided. Also disclosed are methods of making and using the lyophilized compositions and kits supplying the compositions.
Abstract translation: 提供冻干聚合物点组合物。 还公开了制备和使用冻干组合物和提供组合物的试剂盒的方法。
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公开(公告)号：US12272468B2
公开(公告)日：2025-04-08
申请号：US18484587
申请日：2023-10-11
Applicant: University of Washington
Inventor： Daniel T. Chiu , Jiangbo Yu , Yu Rong , Changfeng Wu
Abstract: The present disclosure provides organic-inorganic hybrid polymer particles, which have desirable surface chemistry and optical properties that make them particularly suitable for biological and optical applications. The present disclosure also provides methods of making organic-inorganic hybrid polymer particles. The present disclosure also provides methods of using the organic-inorganic hybrid polymer particles for biological and optical applications.
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公开(公告)号：US20240027325A1
公开(公告)日：2024-01-25
申请号：US18250613
申请日：2021-11-10
Applicant: University of Washington
Inventor： Daniel T. Chiu , Yifei Jiang , Mengxia Zhao , Yuanhua Cheng
IPC: G01N15/14
CPC classification number: G01N15/1434 , G01N2015/0065
Abstract: Systems and methods for analyzing particles flowing in a channel are described. In an embodiment, the channel is configured to flow a particle through a lumen of the channel, the channel defining an interrogation window configured to allow light to pass into and out of the lumen; a light engine comprising: a first light source positioned to output first excitation light onto a first portion of the channel in the interrogation window; and a second light source positioned to output second excitation light onto a second portion of the channel in the interrogation window separate from the first portion. In an embodiment, the systems include an emission fiber bundle comprising a first emission optical fiber and a second emission optical fiber, wherein a proximal end of first emission optical fiber and second emission optical fiber are arranged in an emission fiber bundle head.
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