公开(公告)号：US20180238838A1

公开(公告)日：2018-08-23

申请号：US15553268

申请日：2016-02-25

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Joseph D. Michienzi ,  Martin Gilar ,  Abhijit Tarafder

IPC: G01N30/30 ,  G01N30/34 ,  G01N30/86

CPC classification number: G01N30/30 ,  B01D15/161 ,  G01N30/34 ,  G01N30/461 ,  G01N30/8637 ,  G01N2030/027 ,  G01N2030/3015 ,  G01N2030/3076

Abstract: Methods for focusing analyte peaks in liquid chromatography using a spatial temperature gradient are provided. Also provided are methods for focusing analyte peaks and improving resolution using a trap column upstream of a separation column. Further, methods are provided in which the trap column placed upstream of the separation column is packed with a temperature-sensitive polymer/copolymer, and a spatial temperature gradient is applied along the trap column for obtaining improved retentivity by trap column stationary phase, and overall improved resolution of analyte peaks.

公开(公告)号：US20160139095A1

公开(公告)日：2016-05-19

申请号：US14898272

申请日：2014-06-17

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Geoff C. Gerhardt ,  Martin Gilar ,  Bernard Bunner

IPC: G01N30/86

CPC classification number: G01N30/8668 ,  G01N30/6095 ,  G01N30/8665 ,  G01N2030/027

Abstract: Provided are systems and methods for adapting to volume variations in microfluidic chromatography columns. A column is calibrated by comparing a parameter of the column with a same parameter of a reference column and generating, by a processor, an adjustment factor in response to the comparison between the parameter of the column with a same parameter of the reference column. Volume differences between the calibrated column and the reference column are compensated for by integrating the generated adjustment factor into a sample separation involving the calibrated column.

Abstract translation: 提供了适应微流体色谱柱体积变化的系统和方法。 通过将列的参数与参考列的相同参数进行比较来校准列，并且响应于列的参数与参考列的相同参数之间的比较，由处理器生成调整因子。 通过将生成的调整因子整合到涉及校准柱的样品分离中来补偿校准柱和参考柱之间的体积差异。

公开(公告)号：US20160082435A1

公开(公告)日：2016-03-24

申请号：US14489855

申请日：2014-09-18

Applicant: Waters Technologies Corporation

Inventor： Bernard Bunner ,  Martin Gilar ,  Robert A. Jencks ,  Charles T. Murphy

IPC: B01L3/00

CPC classification number: B01L3/502753 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502746 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2400/0487 ,  B01L2400/086 ,  G01N30/603 ,  G01N30/6095

Abstract: A system that incorporates teachings of the subject disclosure may include, for example, a process that includes obtaining a porous medium comprising a porous material having a first shape and an initial porosity profile. The porous medium is engaged with a cavity in a fluidic device, wherein the cavity is in fluid communication with a channel of the fluidic device. The first shape of the porous material can be adjusted to a second shape resulting in the initial porosity profile being adjusted to a target porosity profile. Such adjustment can be accomplished by the engaging of the porous medium with the cavity, by pre-adjusting a shape of the porous media before insertion into the cavity, or by some combination thereof. Other embodiments are disclosed.

Abstract translation: 结合本公开的教导的系统可以包括例如包括获得包含具有第一形状和初始孔隙率分布的多孔材料的多孔介质的方法。 多孔介质与流体装置中的空腔接合，其中空腔与流体装置的通道流体连通。 可以将多孔材料的第一形状调节到第二形状，从而将初始孔隙率分布调整到目标孔隙率分布。 这种调节可以通过将多孔介质与空腔接合，通过在插入空腔之前预先调节多孔介质的形状，或通过其一些组合来实现。 公开了其他实施例。

公开(公告)号：US12025593B2

公开(公告)日：2024-07-02

申请号：US17095280

申请日：2020-11-11

Applicant: Waters Technologies Corporation

Inventor： Jason F. Hill ,  Sylvain Gilles Cormier ,  Martin Gilar

IPC: G01N30/20 ,  G01N30/10 ,  G01N30/30 ,  G01N30/34 ,  G01N30/02

CPC classification number: G01N30/20 ,  G01N30/10 ,  G01N30/34 ,  G01N2030/027 ,  G01N2030/201 ,  G01N2030/202

Abstract: Described herein are a selectable flow restriction device and a fluidic network for chromatographic sample dilution during injection. A selectable flow restriction device is used in combination with a splitter to define an adjustable splitter for separating a high-pressure solvent flow into two separate solvent flows having the same solvent composition. The first solvent flow passes to a sample manager where a sample is injected as a discrete fluidic plug at an injection valve into a flow comprising at least one solvent. The second solvent flow is merged downstream from the location of injection with the first solvent carrying the sample fluidic plug. The second solvent flow dilutes the sample plug during the mixture of the two solvent flows.

公开(公告)号：US11918936B2

公开(公告)日：2024-03-05

申请号：US17150911

申请日：2021-01-15

Applicant: Waters Technologies Corporation

Inventor： Michael Donegan ,  Martin Gilar ,  Armand Gatien Ngounou Wetie

IPC: B01D15/38 ,  G01N30/34

CPC classification number: B01D15/3828 ,  G01N30/34

Abstract: The present disclosure discusses a method of separating a sample including oligonucleotides including coating a flow path of a chromatographic system; injecting the sample comprising oligonucleotides into the chromatographic system; flowing the sample through the chromatographic system; and separating the oligonucleotides. In some examples, the coating of the flow path is non-binding with respect to the analyte, such as oligonucleotides. Consequently, the analyte does not bind to the coating of the flow path. The non-binding coating eliminates the need for passivation, which can eliminate the formerly needed time to passivate as well. In addition, analyte can be recovered with a first injection in a system, such as chromatographic system.

公开(公告)号：US11912983B2

公开(公告)日：2024-02-27

申请号：US17112502

申请日：2020-12-04

Applicant: Waters Technologies Corporation

Inventor： Michael Donegan ,  Martin Gilar ,  Matthew Lauber ,  Scott Mccall ,  Pamela Iraneta ,  Kerri Smith

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/101 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/315

Abstract: The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.

公开(公告)号：US20220017887A1

公开(公告)日：2022-01-20

申请号：US17375325

申请日：2021-07-14

Applicant: Waters Technologies Corporation

Inventor： Michael Donegan ,  Martin Gilar ,  Darryl W. Brousmiche

IPC: C12N15/10 ,  B01J41/10 ,  B01J41/09

Abstract: The present disclosure relates to compositions, kits and methods that may be used for removal of matrix components, including proteins and lipids, from one or more oligonucleotides.

公开(公告)号：US20210396721A1

公开(公告)日：2021-12-23

申请号：US17354234

申请日：2021-06-22

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Martin Gilar ,  Joseph D. Michienzi ,  Wade P. Leveille ,  Geoff C. Gerhardt ,  Jeffrey Musacchio ,  Jason F. Hill ,  Sylvain G. Cormier

IPC: G01N30/46 ,  G01N30/32 ,  G01N30/30

Abstract: The exemplary embodiments may provide a collective insulating sleeve for a plurality of chromatography columns or may provide separate insulating sleeve for each of the chromatography columns in a plurality. As a result, column ovens are not needed, and pre-heaters may not be required for each chromatography column in some exemplary embodiments. Thus, parallel column arrangements in the exemplary embodiments may be more compact than conventional arrangements.

公开(公告)号：US11105778B2

公开(公告)日：2021-08-31

申请号：US16098280

申请日：2017-05-04

Applicant: Waters Technologies Corporation

Inventor： Martin Gilar ,  Richard W. Andrews ,  Peyton C. Beals ,  Thomas McDonald ,  Keith Fadgen ,  Jordan Kornfeld ,  Geoff Gerhardt ,  Sylvain Cormier

IPC: G01N30/24 ,  G01N30/06 ,  G01N30/20 ,  G01N30/34 ,  G01N1/38

Abstract: A method for injecting a diluted sample in a chromatography system includes merging a flow of a sample and a flow of a diluent to form a flow of a diluted sample. A dilution ratio of the diluted sample equals a sum of the volumetric flow rates of the sample and the diluent divided by the volumetric flow rate of the sample. The diluted sample is stored in a holding element before injection into a chromatographic system flow. Sample dilution occurs under low pressure relative to the chromatographic flow thereby allowing lower pressure sample and diluent syringes to be used. Other benefits include reduced compressibility and a reduction in leaks due to the lower pressure operation. The method avoids problems associated with manual techniques which can introduce errors due, for example, to loss of sample, sample precipitation and adsorption of sample to vials.

公开(公告)号：US20210220755A1

公开(公告)日：2021-07-22

申请号：US17150911

申请日：2021-01-15

Applicant: Waters Technologies Corporation

Inventor： Michael Donegan ,  Martin Gilar ,  Armand Gatien Ngounou Wetie

IPC: B01D15/38 ,  G01N30/34

Abstract: The present disclosure discusses a method of separating a sample including oligonucleotides including coating a flow path of a chromatographic system; injecting the sample comprising oligonucleotides into the chromatographic system; flowing the sample through the chromatographic system; and separating the oligonucleotides. In some examples, the coating of the flow path is non-binding with respect to the analyte, such as oligonucleotides. Consequently, the analyte does not bind to the coating of the flow path. The non-binding coating eliminates the need for passivation, which can eliminate the formerly needed time to passivate as well. In addition, analyte can be recovered with a first injection in a system, such as chromatographic system.