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公开(公告)号：US06702943B1
公开(公告)日：2004-03-09
申请号：US10130958
申请日：2002-09-16
IPC分类号： B01D1508
CPC分类号： B01J41/20 , B01D15/327 , B01J41/09 , C02F1/42
摘要： A method for the removal of a substance carrying a negative charge and being present in an aqueous liquid (I). The method comprises the steps of: (i) contacting the liquid with a matrix carrying a plurality of ligands comprising a positively charged structure and a hydrophobic structure, and (ii) desorbing the substance. The characterizing feature is that (I) each of said ligands together with a spacer has the formula: —SP—[Ar—R1—N+(R2R3R4)] where (A) [Ar—R1—N+(R2R3R4)] represents a ligand a) Ar is an aromatic ring, b) R1 is [(L)nR′1]m where n and m are integers selected amongst zero or 1; L is amino nitrogen, ether oxygen or thioether sulphur; R′1 is a linker selected among 1) hydrocarbon groups; 2) —C(═NH)—; c) R2-4 are selected among hydrogen and alkyls; (B) SP is a spacer providing a carbon or a heteroatom directly attached to Ar—R1—N+(R2R3R4); (C)—represents that SP replaces a hydrogen in (Ar—R1—N+(R2R3R4); (D)—represents binding to the matrix; and (II) desorption. There is also described (a) anion-exchangers having high breakthrough capacities, (b) a screening method and (c) a desalting protocol.
摘要翻译： 一种用于去除携带负电荷并存在于水性液体（I）中的物质的方法。 该方法包括以下步骤：（i）使液体与承载包含带正电结构和疏水结构的多个配体的基质接触，和（ii）解吸该物质。 其特征在于：（I）每个所述配体与间隔基一起具有下式：-SP- [Ar-R1-N +（R2R3R4）]其中（A）[Ar-R1-N +（ R2R3R4）]表示配体a）Ar是芳环，b）R1是[（L）nR'1] m，其中n和m是选自零或1的整数; L为氨基氮，醚氧或硫醚硫; R'1是选自1）烃基的连接体; 2）-C（= NH） - ; c）R2-4选自氢和烷基; （B）SP是提供直接与Ar-R 1 -N +（R 2 R 3 R 4）连接的碳或杂原子的间隔基; （C） - 表示SP替代（Ar-R1-N +（R2R3R4）中的氢;（D） - 表示与基质的结合;和（II）解吸，还描述了（a）阴离子交换 具有较高的突破能力，（b）筛选方法和（c）脱盐方案。
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公开(公告)号：US09266041B2
公开(公告)日：2016-02-23
申请号：US13158780
申请日：2011-06-13
申请人： Bo-Lennart Johansson , Jean-Luc Maloisel , Hans J. Johansson , Asa Ek , Maria Holm , Jafar Saidzadeh
发明人： Bo-Lennart Johansson , Jean-Luc Maloisel , Hans J. Johansson , Asa Ek , Maria Holm , Jafar Saidzadeh
IPC分类号： C07K1/14 , B01D15/32 , B01D15/36 , B01D15/38 , B01J20/28 , B01J20/286 , B01J20/32 , B01J41/20 , C07K1/16 , C07K1/18 , C07K1/36 , C07K16/00 , C07K16/06
CPC分类号： B01D15/327 , B01D15/363 , B01D15/3804 , B01J20/28033 , B01J20/286 , B01J20/3219 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/16 , C07K1/18 , C07K1/36 , C07K16/00 , C07K16/065 , Y10T29/49826
摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
摘要翻译： 本发明涉及由下式R1-R2-N（R3）-R4-R5定义的色谱配体，其中R1是取代或未取代的苯基; R2是包含0-4个碳原子的烃链; R3是包含1-3个碳原子的烃链; R4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜耦合的所述配体。 根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。
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公开(公告)号：US09044740B2
公开(公告)日：2015-06-02
申请号：US12594002
申请日：2008-03-20
IPC分类号： C07K16/00 , C07H1/00 , B01D15/38 , B01D15/08 , B01J20/289 , B01D15/32 , B01J20/286 , B01J20/32 , C07K1/20 , B01J20/26 , B01D15/36
CPC分类号： B01J20/289 , B01D15/32 , B01D15/327 , B01D15/361 , B01D15/3804 , B01D15/3847 , B01J20/262 , B01J20/267 , B01J20/286 , B01J20/3212 , B01J20/3219 , B01J20/3251 , B01J20/3272 , B01J20/328 , B01J20/3285 , B01J2220/54 , B01J2220/58 , C07K1/20 , C07K16/00
摘要： The present invention relates to a separation matrix comprised of a porous or non-porous support to which ligands have been immobilized, wherein said ligands comprise at least one aliphatic sulfamide. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulins, such as IgG, Fab fragments, fusion proteins containing immunoglobulins etc, by adsorption to a separation matrix that comprises the aliphatic sulfamide ligands of the invention.
摘要翻译： 本发明涉及由已经固定有配体的多孔或无孔载体组成的分离基质，其中所述配体包含至少一个脂族磺酰胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到包含脂族磺酰胺配体的分离基质上分离免疫球蛋白如IgG，Fab片段，含有免疫球蛋白的融合蛋白等的方法 的本发明。
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公开(公告)号：US08685248B2
公开(公告)日：2014-04-01
申请号：US12709556
申请日：2010-02-22
IPC分类号： B01D15/08
CPC分类号： B01J20/262 , B01J20/267 , B01J20/28019 , B01J20/28023 , B01J20/28033 , B01J20/28042 , B01J20/286 , B01J20/289 , B01J20/3092 , B01J20/3208 , B01J20/3212 , B01J20/3219 , B01J20/3246 , B01J20/3248 , B01J20/3251 , B01J20/3255 , B01J20/3272 , B01J20/3285 , B01J2220/54 , B01J2220/58 , C07K16/065 , Y10T428/249963
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one aliphatic sulphonamide. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to a separation matrix that comprises aliphatic sulphonamide ligands.
摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一种脂族磺酰胺。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到包含脂族磺酰胺配体的分离基质上分离免疫球蛋白样化合物的方法。
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公开(公告)号：US08574437B2
公开(公告)日：2013-11-05
申请号：US13575750
申请日：2011-02-16
IPC分类号： B01D15/08
CPC分类号： B01J20/3293 , B01D15/361 , B01D15/3804 , B01J20/28004 , B01J20/286 , B01J20/3206 , B01J20/3248 , B01J20/3285 , B01J39/26 , B01J41/20 , C07K1/18
摘要： The present invention relates to a method of producing novel chromatography media and use thereof for purification of biomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially to advantageous for production of chromatofocusing media.
摘要翻译： 本发明涉及生产新型色谱介质的方法及其用于生物分子如蛋白质的纯化的用途。 色谱介质包括具有内多孔芯和外壳的壳珠。 该方法包括在珠的核心中提供缓冲配体，并提供靶向用于珠粒外壳中的生物分子结合的结合配体。 该方法使得可以彼此独立地优化结合性质和缓冲性质，这特别有利于生产色谱聚焦介质。
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公开(公告)号：US08372286B2
公开(公告)日：2013-02-12
申请号：US12863448
申请日：2009-01-30
IPC分类号： B01D15/08
CPC分类号： B01J20/291 , B01J20/262 , B01J20/2809 , B01J20/285 , B01J20/286 , B01J20/287 , B01J20/288 , B01J20/3212 , B01J20/3268 , B01J20/3274 , B01J20/3293 , B01J39/26 , B01J41/20 , B01J2220/52
摘要： The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.
摘要翻译： 本发明涉及使用所谓的旋转圆盘技术生产分离介质的方法，其中珠子的孔隙度以使得所需生物分子可以与复杂样品分离的方式进行优化。 该方法包括以下步骤：a）将具有350-450mPa·s粘度的4-8％多糖溶液在65-75℃下以3001-3010rpm向一个或多个纺丝盘送入，以形成多糖珠粒 ; b）在捕获浴中捕获所述形成的多糖珠粒; 其中多糖珠的孔隙率通过在15和27℃之间，优选在17.5和24.6℃之间改变捕获温度来控制。该方法产生阻止大于150000g / mol的分子扩散到 珠子 本发明还涉及通过其用于纯化生物分子，特别是单克隆抗体的方法和用途产生的分离培养基。
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公开(公告)号：US08114280B2
公开(公告)日：2012-02-14
申请号：US11570530
申请日：2005-06-22
IPC分类号： B01D15/08
CPC分类号： B01J20/3212 , B01D15/426 , B01J20/286 , B01J20/289 , B01J20/3219 , B01J20/3251
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.
摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一个磺酰胺，并且磺酰基的R基包括芳族基团。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到分离基质上分离免疫球蛋白样化合物的方法。
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公开(公告)号：US07867784B2
公开(公告)日：2011-01-11
申请号：US11577208
申请日：2005-10-21
申请人： Carina Engstrand , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J. Johansson , Jean-Luc Maloisel
发明人： Carina Engstrand , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J. Johansson , Jean-Luc Maloisel
IPC分类号： G01N33/544 , G01N33/548 , G01N33/551 , C12M1/34 , C07C211/44
CPC分类号： B01D15/3828 , B01D15/327 , B01D15/363 , B01D15/3804 , B01D15/3809 , B01D15/3847 , B01J20/286 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/165 , C07K1/36 , C07K16/00 , C07K16/065
摘要： The present invention relates to a chromatography ligand defined by the following formula (I) R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
摘要翻译： 本发明涉及由下式（I）R1-R2-N（R3）-R4-R5定义的色谱配体，其中R1是取代或未取代的苯基; R2是包含0-4个碳原子的烃链; R3是包含1-3个碳原子的烃链; R4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜耦合的所述配体。 根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。
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公开(公告)号：US20100298548A1
公开(公告)日：2010-11-25
申请号：US12863448
申请日：2009-01-30
CPC分类号： B01J20/291 , B01J20/262 , B01J20/2809 , B01J20/285 , B01J20/286 , B01J20/287 , B01J20/288 , B01J20/3212 , B01J20/3268 , B01J20/3274 , B01J20/3293 , B01J39/26 , B01J41/20 , B01J2220/52
摘要： The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.
摘要翻译： 本发明涉及使用所谓的旋转圆盘技术生产分离介质的方法，其中珠子的孔隙度以使得所需生物分子可以与复杂样品分离的方式进行优化。 该方法包括以下步骤：a）将具有350-450mPa·s粘度的4-8％多糖溶液在65-75℃下以3001-3010rpm向一个或多个纺丝盘送入，以形成多糖珠 ; b）在捕获浴中捕获所述形成的多糖珠粒; 其中多糖珠的孔隙率通过在15和27℃之间，优选在17.5和24.6℃之间改变捕获温度来控制。该方法产生阻止大于150000g / mol的分子扩散到 珠子 本发明还涉及通过其用于纯化生物分子，特别是单克隆抗体的方法和用途产生的分离培养基。
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公开(公告)号：US20070112178A1
公开(公告)日：2007-05-17
申请号：US10589718
申请日：2005-02-24
CPC分类号： C07K1/18 , B01D15/362 , B01D15/3809 , B01D15/3847 , B01J39/07 , B01J39/26 , B01J47/014 , C07K1/165 , B01D15/327
摘要： The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.
摘要翻译： 本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个 阳离子交换基团和至少一个芳族或杂芳族环系统。 在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。 本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。
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