公开(公告)号：US07169277B2

公开(公告)日：2007-01-30

申请号：US09919505

申请日：2001-07-31

申请人： Walter Ausserer ,  Luc J. Bousse ,  Robert S. Dubrow ,  Steven A. Sundberg ,  Andrea W. Chow ,  Benjamin N. Wang

发明人： Walter Ausserer ,  Luc J. Bousse ,  Robert S. Dubrow ,  Steven A. Sundberg ,  Andrea W. Chow ,  Benjamin N. Wang

IPC分类号： G01N27/26

CPC分类号： G01N27/44791 ,  G01N27/44743

摘要： Devices, systems and methods for use in separating sample materials into different fractions that employ bulk fluid flow for loading of samples followed by electrophoretic separation of the sample material. Devices employ configurations that optionally allow bulk sample loading with some or no displacement of a separation matrix within a separation conduit. Methods of using these devices, and systems that incorporate these devices are also envisioned.

摘要翻译： 用于将样品材料分离成不同级分的装置，系统和方法，其使用大量流体流来装载样品，然后电泳分离样品材料。 设备采用可选地允许在分离管道内部分或不排出分离基质的大量样品加载的构造。 也可以设想使用这些装置的方法以及包含这些装置的系统。

公开(公告)号：US6149787A

公开(公告)日：2000-11-21

申请号：US173469

申请日：1998-10-14

申请人： Andrea W. Chow ,  Robert S. Dubrow ,  J. Wallace Parce ,  Steven A. Sundberg ,  Jeffrey A. Wolk

发明人： Andrea W. Chow ,  Robert S. Dubrow ,  J. Wallace Parce ,  Steven A. Sundberg ,  Jeffrey A. Wolk

IPC分类号： B01L3/00 ,  G01N35/00 ,  G01N35/10 ,  G01N27/26

CPC分类号： B01L3/502715 ,  B01L3/502746 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/084 ,  B01L3/50273 ,  G01N2035/00237 ,  G01N2035/1062 ,  Y10S366/02 ,  Y10T436/2575

摘要： Methods, apparatus and systems are provided for introducing large numbers of different materials into a microfluidic analytical device rapidly, efficiently and reproducibly. In particular, improved integrated pipettor chip configurations, e.g. sippers or electropipettors, are described which are capable of sampling extremely small amounts of material for which analysis is desired, transporting material into a microfluidic analytical channel network, and performing the desired analysis on the material.

公开(公告)号：US5684636A

公开(公告)日：1997-11-04

申请号：US518651

申请日：1995-08-24

申请人： Andrea W. Chow ,  Hope B. Warren ,  Susan P. Ermer

发明人： Andrea W. Chow ,  Hope B. Warren ,  Susan P. Ermer

IPC分类号： G02B3/12

CPC分类号： G02B3/12

摘要： A polymer composition is provided for use as a liquid lens element in a compound lens designed to reduce chromatic aberration. The polymer composition (i) has an abnormal optical dispersion and (ii) is more easily fabricated into the compound lens than a conventional optical liquid. The composition contains an abnormal optical liquid characterized by an abnormal optical dispersion and a polymer component. The polymer is soluble in the optical liquid and is present in an amount effective to impart a viscosity that is at least an order of magnitude greater than that of the liquid alone at a selected temperature.

摘要翻译： 聚合物组合物被提供用于复合透镜中用作液体透镜元件，用于减小色差。 聚合物组合物（i）具有异常的光学分散体，和（ii）比传统的光学液体更容易地制成复合透镜。 该组合物含有以异常光学分散体和聚合物组分为特征的异常光学液体。 聚合物可溶于光学液体中，并且以有效量的量存在，该粘度比选定温度下单独液体的粘度至少大一个数量级。

公开(公告)号：US08241883B2

公开(公告)日：2012-08-14

申请号：US11412055

申请日：2006-04-25

申请人： Andrea W. Chow ,  John C. Owicki ,  J. Wallace Parce

发明人： Andrea W. Chow ,  John C. Owicki ,  J. Wallace Parce

IPC分类号： C12N13/00

CPC分类号： G01N33/557 ,  B01L3/50273 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/1822 ,  B01L2300/1833 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0487 ,  G01N27/44791 ,  G01N33/5306

摘要： The present invention provides novel microfluidic devices and methods for performing pulsed field mobility shift assays in microfluidic devices. In particular the devices and methods of the invention utilize differences between electrophoretic mobilities (e.g., as between reactants and products, especially in non-fluorogenic reactions) in order to separate the species and thus analyze the reaction.

摘要翻译： 本发明提供了用于在微流体装置中进行脉冲场迁移率变动测定的新型微流体装置和方法。 特别地，本发明的装置和方法利用电泳迁移率之间的差异（例如，在反应物和产物之间，特别是在非荧光反应中），以便分离物种并因此分析反应。

公开(公告)号：US08216852B2

公开(公告)日：2012-07-10

申请号：US12947963

申请日：2010-11-17

申请人： Andrea W. Chow ,  H. Garrett Wada

发明人： Andrea W. Chow ,  H. Garrett Wada

IPC分类号： G01N1/00

CPC分类号： G01N27/44743 ,  B01L3/502707 ,  B01L3/502746 ,  B01L2300/0816 ,  B01L2300/0858 ,  B01L2300/0877 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/084 ,  Y10T436/25 ,  Y10T436/2575

摘要： The present invention provides novel methods for controlling/manipulating materials flowing in a fluidic device. In particular, the methods of the invention create and utilize differences between dispersion rates and/or average velocity of materials in order to manipulate the materials.

摘要翻译： 本发明提供用于控制/操纵在流体装置中流动的材料的新颖方法。 特别地，本发明的方法产生和利用了扩散速率和/或材料的平均速度之间的差异来操纵材料。

公开(公告)号：US06915679B2

公开(公告)日：2005-07-12

申请号：US09792435

申请日：2001-02-23

申请人： Ring-Ling Chien ,  J. Wallace Parce ,  Andrea W. Chow ,  Anne Kopf-Sill

发明人： Ring-Ling Chien ,  J. Wallace Parce ,  Andrea W. Chow ,  Anne Kopf-Sill

IPC分类号： G01N11/04 ,  B01L3/00 ,  B01L9/00 ,  G01N11/08 ,  G01N27/06 ,  G01N27/447 ,  G01N35/08 ,  G01N35/10 ,  G01N37/00 ,  G05D7/06 ,  G01N11/00

CPC分类号： B01L9/527 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N11/08 ,  G01N27/44791 ,  G01N2035/1044 ,  G05D7/0694 ,  Y10T137/2076 ,  Y10T137/218 ,  Y10T137/86131

摘要： Improved microfluidic devices, systems, and methods allow selective transportation of fluids within microfluidic channels of a microfluidic network by applying, controlling, and varying pressures at a plurality of reservoirs. Modeling the microfluidic network as a series of nodes connected together by channel segments and determining the flow resistance characteristics of the channel segments may allow calculation of fluid flows through the channel segments resulting from a given pressure configuration at the reservoirs. To effect a desired flow within a particular channel or series of channels, reservoir pressures may be identified using the network model. Viscometers or other flow sensors may measure flow characteristics within the channels, and the measured flow characteristics can be used to calculate pressures to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems can optionally be used in conjunction with electrokinetic or other fluid transport mechanisms.

公开(公告)号：US06589729B2

公开(公告)日：2003-07-08

申请号：US09774531

申请日：2001-01-31

申请人： Samuel D. H. Chan ,  Ring-Ling Chien ,  Andrea W. Chow ,  Benjamin N. Wang

发明人： Samuel D. H. Chan ,  Ring-Ling Chien ,  Andrea W. Chow ,  Benjamin N. Wang

IPC分类号： C12Q100

CPC分类号： G01N21/64 ,  B01J19/0093 ,  B01L3/5027 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/084 ,  G01N21/6428 ,  G01N21/75 ,  G01N30/02 ,  G01N2021/6482 ,  B01D15/3857

摘要： Methods for monitoring time dependent reactions that comprise providing a flow channel, typically microscale in dimension, flowing at least two reagents into the flow channel and varying the flow rate of the mixture through the flow channel. By increasing and/or decreasing the flow rate of the reagent mixture from the point of mixing to the point of detection, one alters the amount of reaction time, allowing monitoring reaction kinetics over time.

摘要翻译： 用于监测时间依赖性反应的方法，其包括提供流动通道，尺寸通常为微尺寸，将至少两种试剂流入流动通道并改变混合物通过流动通道的流速。 通过增加和/或降低试剂混合物从混合点到检测点的流速，可以改变反应时间的量，从而监测反应动力学随时间的变化。

公开(公告)号：US20120273353A1

公开(公告)日：2012-11-01

申请号：US13544006

申请日：2012-07-09

申请人： Andrea W. Chow ,  John C. Owicki ,  J. Wallace Parce

发明人： Andrea W. Chow ,  John C. Owicki ,  J. Wallace Parce

IPC分类号： G01N27/447

CPC分类号： G01N33/557 ,  B01L3/50273 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/1822 ,  B01L2300/1833 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0487 ,  G01N27/44791 ,  G01N33/5306

摘要： The present invention provides novel methods for performing pulsed field mobility shift assays in microfluidic devices. In particular, the methods of the invention utilize differences between electrophoretic mobilities (e.g., as between reactants and products, especially in non-fluorogenic reactions) in order to separate the species and thus analyze the reaction.

摘要翻译： 本发明提供了用于在微流体装置中进行脉冲场迁移率变动测定的新方法。 特别地，本发明的方法利用电泳迁移率之间的差异（例如，在反应物和产物之间，特别是在非荧光反应中），以便分离物质并由此分析反应。

公开(公告)号：US07247274B1

公开(公告)日：2007-07-24

申请号：US10292401

申请日：2002-11-12

申请人： Andrea W. Chow

发明人： Andrea W. Chow

IPC分类号： B01L3/02 ,  B32B5/02 ,  B32B27/04 ,  B32B27/12 ,  G01N15/06

CPC分类号： B01F13/0059 ,  B01F5/0471 ,  B01F2015/0221 ,  B01J19/0093 ,  B01J2219/00247 ,  B01J2219/00783 ,  B01J2219/0086 ,  B01L3/5027 ,  B01L3/502746 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1833 ,  B01L2300/1838 ,  B01L2300/1861 ,  B01L2400/0415 ,  B01L2400/0487 ,  Y10T137/0329 ,  Y10T137/0357 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/13 ,  Y10T436/25625 ,  Y10T436/2575

摘要： The present invention provides novel microfluidic devices and methods for preventing/ameliorating formation of precipitate blockages in microfluidic devices. In particular the devices and methods of the invention utilize microchannels of specific cross-sectional configuration and of specific arrangement as well as application of AC current orthogonal to the direction of fluid flow, in order to preventing/ameliorating formation of precipitate blockages in microfluidic devices.

摘要翻译： 本发明提供了用于预防/改善微流体装置中沉淀物阻塞的形成的新型微流体装置和方法。 特别地，本发明的装置和方法利用具有特定横截面构造和具体布置的微通道以及与流体流动方向正交的AC电流的应用，以便防止/改善微流体装置中沉淀物堵塞的形成。

公开(公告)号：US06779559B2

公开(公告)日：2004-08-24

申请号：US10702069

申请日：2003-11-05

申请人： J. Wallace Parce ,  Andrea W. Chow

发明人： J. Wallace Parce ,  Andrea W. Chow

IPC分类号： F15C118

CPC分类号： F15C1/04 ,  B01L3/02 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/06 ,  Y10T137/0396 ,  Y10T137/2076 ,  Y10T137/2142 ,  Y10T137/218 ,  Y10T137/2191 ,  Y10T137/2273

摘要： Methods devices and systems that employ non-mechanical valve modules for controlling directing fluid and other material movement through integrated microscale channel network. These non-mechanical valve modules apply forces that counter the driving forces existing through a given channel segment, via fluidly connected channel segments, so as to selectively arrest flow of material within the given channel segment.

摘要翻译： 方法使用非机械阀模块来控制引导流体和其他材料通过集成微型通道网络运动的装置和系统。 这些非机械阀模块通过流体连接的通道段施加抵抗通过给定通道段存在的驱动力的力，以便选择性地阻止给定通道段内的材料流。