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179 条记录 (耗时 0.054 秒)

    Stabilizing proteins against denaturation and inactivation by charged detergents using chemical modifications, including modifications that increase net charge
    41.
    发明申请
    Stabilizing proteins against denaturation and inactivation by charged detergents using chemical modifications, including modifications that increase net charge 审中-公开
    通过使用化学修饰，包括增加净电荷的修饰，通过加入的洗涤剂使稳定的蛋白质变性和失活

    公开(公告)号：US20080113421A1

    公开(公告)日：2008-05-15

    申请号：US11637203

    申请日：2006-12-11

    申请人： Bryan F. Shaw ,  Katherine L. Gudiksen ,  Irina Gitlin ,  Demetri T. Moustakas ,  George M. Whitesides

    发明人： Bryan F. Shaw ,  Katherine L. Gudiksen ,  Irina Gitlin ,  Demetri T. Moustakas ,  George M. Whitesides

    IPC分类号： C12N9/96

    CPC分类号： C12N9/96 ,  C11D3/386

    摘要： The present invention generally relates to enzymes and other proteins resistant to denaturation, and techniques for making and using the same. In one aspect, lysine and/or other charged residues within an enzyme are reacted in some fashion, which can render the enzyme more resistant to denaturation. For example, the lysine residue may be neutralized by acetylating the residue, for instance, by exposure to acetic anhydride. In some aspects, the enzyme, after reaction, may be relatively resistant to degradation when placed in a harsh environment, for example, when exposed to sodium dodecyl sulfate at a concentration of at least about 2.5 mM in Tris-Gly buffer. The enzyme may still be susceptible to denaturation in some cases, but at a much slower rate (e.g., the denaturation time constant may be higher). Other aspects of the invention are directed to enzymes prepared in such fashion, methods of promoting or using such enzymes, kits involving such enzymes, and the like.

    摘要翻译： 本发明一般涉及对变性有抗性的酶和其它蛋白质，以及制备和使用它们的技术。 在一个方面，酶内的赖氨酸和/或其他带电残基以某种方式反应，这可使酶对变性更具抗性。 例如，赖氨酸残基可以通过乙酰化残基例如通过暴露于乙酸酐来中和。 在一些方面，当置于恶劣环境中时，例如当暴露于Tris-Gly缓冲液中至少约2.5mM浓度的十二烷基硫酸钠时，反应后的酶可能相对抗降解。 在某些情况下，该酶可能仍然易于变性，但是以较慢的速率（例如，变性时间常数可能更高）。 本发明的其它方面涉及以这种方式制备的酶，促进或使用这些酶的方法，涉及这种酶的试剂盒等。

    Molecular recognition at surfaces derivatized with self-assembled monolayers
    45.
    发明授权
    Molecular recognition at surfaces derivatized with self-assembled monolayers 有权
    用自组装单层衍生的表面的分子识别

    公开(公告)号：US06197515B1

    公开(公告)日：2001-03-06

    申请号：US09296111

    申请日：1999-04-21

    申请人： Cynthia C. Bamdad ,  George B. Sigal ,  Jack L. Strominger ,  George M. Whitesides

    发明人： Cynthia C. Bamdad ,  George B. Sigal ,  Jack L. Strominger ,  George M. Whitesides

    IPC分类号： C12Q168

    CPC分类号： B82Y30/00 ,  B82Y15/00 ,  B82Y40/00 ,  C07H21/04 ,  C12Q1/6837 ,  G01N33/54353 ,  G01N33/54373 ,  G01N33/553 ,  G01N2610/00

    摘要： An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.

    摘要翻译： 适合用作生物传感器的制品包括作为自组装单层的一部分粘附到制品表面的式X-R-Ch分子。 X是附着于表面的官能团，R是间隔基，Ch是螯合剂。 金属离子可以由螯合剂配位，并且靶向生物分子的聚氨基酸标记的生物结合配偶体与金属离子配位。 本发明的方法包括使制品与含有或怀疑含有靶生物分子的培养基接触并允许生物分子与结合配偶体生物结合。 该文章特别用作表面等离子体共振芯片。

    Microcontact printing of catalytic colloids
    47.
    发明授权
    Microcontact printing of catalytic colloids 失效
    催化胶体的微接触印刷

    公开(公告)号：US6060121A

    公开(公告)日：2000-05-09

    申请号：US616692

    申请日：1996-03-15

    申请人： Pirmin Hidber ,  Wolfgang Helbig ,  Enoch Kim ,  George M. Whitesides

    发明人： Pirmin Hidber ,  Wolfgang Helbig ,  Enoch Kim ,  George M. Whitesides

    IPC分类号： C23C18/16 ,  G03F7/00 ,  H05K3/18 ,  B05D5/00

    CPC分类号： G03F7/0002 ,  B82Y10/00 ,  B82Y30/00 ,  B82Y40/00 ,  C23C18/1608 ,  H05K3/182 ,  B41M1/04 ,  B41M1/40 ,  B41M3/006

    摘要： A method of transferring a chemical activating agent from an applicator to a substrate surface involves providing an applicator having a raised application surface, applying a chemical activating agent to the application surface, contacting the substrate surface with the application surface, and removing the applicator. The chemical activating agent is transferred in a form in which it is capable of effecting a chemical reaction, such as catalysis, at the surface. Thus, following transfer of the chemical activating agent to the surface in a pattern, a metal can be plated at the substrate surface in the pattern. The applicator can be an elastomeric stamp having a raised portion defining a stamping surface.

    摘要翻译： 将化学活化剂从施用器转移到基底表面的方法包括提供具有凸起的施加表面的施用器，向施加表面施加化学活化剂，将基底表面与施加表面接触，以及移除施用器。 化学活化剂以其能够在表面进行化学反应（例如催化）的形式转移。 因此，在以图案将化学活化剂转移到表面之后，可以以图案在基板表面上镀金属。 涂抹器可以是具有限定冲压表面的凸起部分的弹性体印模。

    Method for preparing adenosine triphosphate
    49.
    发明授权
    Method for preparing adenosine triphosphate 失效
    三磷酸腺苷的制备方法

    公开(公告)号：US4164444A

    公开(公告)日：1979-08-14

    申请号：US764516

    申请日：1977-01-31

    申请人： George M. Whitesides ,  Patricia E. Garrett ,  Merrell G. Siegel

    发明人： George M. Whitesides ,  Patricia E. Garrett ,  Merrell G. Siegel

    IPC分类号： C07F9/09 ,  C12P19/32 ,  C12D13/06 ,  C07H19/20

    CPC分类号： C12P19/32 ,  C07F9/096

    摘要： Acyl phosphate salts suitable for use as phosphate donors in the production of cofactors, such as adenosine triphosphate, are prepared by acylation of phosphoric acid with a ketene, followed by reaction with ammonia or an amine. The reaction product, e.g. diammonium acetyl phosphate, can normally be used directly in enzymic production of cofactors without the necessity of prior conversion to the free acid or another salt, such as the sodium salt. The acyl phosphate salts are produced in high yields, and the method allows production of cofactors for use in biosynthesis from readily available materials.

    摘要翻译： 通过用乙烯酮酰化磷酸，随后与氨或胺反应制备适用于辅助因子如三磷酸腺苷生产中磷酸盐供体的酰基磷酸盐。 反应产物，例如 乙酰基磷酸二铵通常可以直接用于辅助因子的酶产生，而不需要事先转化为游离酸或其他盐，例如钠盐。 以高产率生产酰基磷酸盐，并且该方法允许从容易获得的材料生产用于生物合成的辅因子。

    Production of acyl phosphate salts
    50.
    发明授权
    Production of acyl phosphate salts 失效
    酰基磷酸盐的生产

    公开(公告)号：US4088675A

    公开(公告)日：1978-05-09

    申请号：US666995

    申请日：1976-03-15

    申请人： George M. Whitesides ,  Patricia E. Garrett ,  Merrell G. Siegel

    发明人： George M. Whitesides ,  Patricia E. Garrett ,  Merrell G. Siegel

    IPC分类号： C07F9/09 ,  C07C51/56 ,  C07C87/06 ,  C07F9/06

    CPC分类号： C07F9/096

    摘要： Acyl phosphate salts suitable for use as phosphate donors in the production of cofactors, such as adenosine triphosphate, are prepared by acylation of phosphoric acid with a ketene, followed by reaction with ammonia or an amine. The reaction product, e.g. diammonium acetyl phosphate, can normally be used directly in enzymic production of cofactors without the necessity of prior conversion to the free acid or another salt, such as the sodium salt. The acyl phosphate salts are produced in high yields, and the method allows production of cofactors for use in biosynthesis from readily available materials.

    摘要翻译： 通过用乙烯酮酰化磷酸，随后与氨或胺反应制备适用于辅助因子如三磷酸腺苷生产中磷酸盐供体的酰基磷酸盐。 反应产物，例如 乙酰基磷酸二铵通常可以直接用于辅助因子的酶产生，而不需要事先转化为游离酸或其他盐，例如钠盐。 以高产率生产酰基磷酸盐，并且该方法允许从容易获得的材料生产用于生物合成的辅因子。