公开(公告)号：US20070105132A1

公开(公告)日：2007-05-10

申请号：US11447507

申请日：2006-06-05

申请人： Andrew Berlin ,  Steven Kirch ,  Gabi Neubauer ,  Valluri Rao ,  Mineo Yamakawa

发明人： Andrew Berlin ,  Steven Kirch ,  Gabi Neubauer ,  Valluri Rao ,  Mineo Yamakawa

IPC分类号： C12Q1/68

CPC分类号： G01N21/05 ,  B01L3/5027 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0896 ,  B01L2400/0454 ,  B01L2400/0463 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q1/6869 ,  G01N21/658 ,  G01N2021/0346 ,  G01N2021/651 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656 ,  C12Q2565/628

摘要： The methods and apparatus disclosed herein are useful for detecting nucleotides, nucleosides, and bases and for nucleic acid sequence determination. The methods involve detection of a nucleotide, nucleoside, or base using surface enhanced Raman spectroscopy (SERS). The detection can be part of a nucleic acid sequencing reaction to detect uptake of a deoxynucleotide triphosphate during a nucleic acid polymerization reaction, such as a nucleic acid sequencing reaction. The nucleic acid sequence of a synthesized nascent strand, and the complementary sequence of the template strand, can be determined by tracking the order of incorporation of nucleotides during the polymerization reaction.

摘要翻译： 本文公开的方法和装置可用于检测核苷酸，核苷和碱基并用于核酸序列测定。 该方法涉及使用表面增强拉曼光谱（SERS）检测核苷酸，核苷或碱基。 检测可以是在核酸聚合反应（例如核酸测序反应）期间检测脱氧核苷酸三磷酸的摄取的核酸测序反应的一部分。 合成的新生链的核酸序列和模板链的互补序列可以通过跟踪在聚合反应期间核苷酸掺入的顺序来确定。

公开(公告)号：US20050147981A1

公开(公告)日：2005-07-07

申请号：US10750515

申请日：2003-12-31

申请人： Mineo Yamakawa ,  Andrew Berlin

发明人： Mineo Yamakawa ,  Andrew Berlin

IPC分类号： C12Q1/68 ,  G01Q60/10 ,  G01Q60/50 ,  C12M1/34

CPC分类号： C12Q1/6874 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q2565/601 ,  C12Q2563/155 ,  C12Q2525/179

摘要： A method for determining a nucleotide sequence of a nucleic acid is provided that includes contacting the nucleic acid with a series of labeled oligonucleotides for binding to the nucleic acid, wherein each labeled oligonucleotide includes a known nucleotide sequence and a molecular nanocode. The nanocode of an isolated labeled oligonucleotides that binds to the nucleic acid is then detected using SPM. Nanocodes of the present invention in certain aspects include detectable features beyond the arrangement of tags that encode information about the barcoded object, which assist in detecting the tags that encode information about the barcoded object. The detectable features include structures of a nanocode or associated with a nanocode, referred to herein as detectable feature tags, for error checking/error-correction, encryption, and data reduction/compression.

摘要翻译： 提供了用于确定核酸的核苷酸序列的方法，其包括使核酸与一系列用于结合核酸的标记的寡核苷酸接触，其中每个标记的寡核苷酸包括已知的核苷酸序列和分子纳代码。 然后使用SPM检测与核酸结合的分离的标记寡核苷酸的纳代码。 在某些方面，本发明的纳代码包括超过编码关于条形码对象的信息的标签布置的可检测特征，其有助于检测编码关于条形码对象的信息的标签。 可检测特征包括用于错误校验/纠错，加密和数据缩减/压缩的纳代码或与纳代码相关联的结构，这里称为可检测特征标签。

公开(公告)号：US07302832B2

公开(公告)日：2007-12-04

申请号：US10748526

申请日：2003-12-29

申请人： Andrew Berlin ,  Narayanan Sundararajan ,  Mineo Yamakawa ,  Valluri R. Rao

发明人： Andrew Berlin ,  Narayanan Sundararajan ,  Mineo Yamakawa ,  Valluri R. Rao

IPC分类号： G01N13/12 ,  G01N13/16

CPC分类号： G01Q60/04 ,  G01Q70/06 ,  G01Q70/12 ,  G01Q70/18

摘要： A surface analysis device is disclosed for identifying molecules by simultaneously scanning nanocodes on a surface of a substrate. The device includes a scanning array that is capable of simultaneously scanning the nanocodes on the surface of the substrate and an analyzer that is coupled with the scanning array. The analyzer is capable of receiving simultaneously scanned information from the scanning array and identifying molecules associated with the nanocodes.

摘要翻译： 公开了通过同时扫描基底表面上的纳代码来识别分子的表面分析装置。 该器件包括能够同时扫描衬底表面上的纳代码的扫描阵列和与扫描阵列耦合的分析器。 分析仪能够同时接收来自扫描阵列的扫描信息并识别与纳代码相关的分子。

公开(公告)号：US20060133952A1

公开(公告)日：2006-06-22

申请号：US11317405

申请日：2005-12-22

申请人： Yuegang Zhang ,  Andrew Berlin ,  Qing Ma ,  Li-Peng Wang ,  Valluri Rao ,  Mineo Yamakawa

发明人： Yuegang Zhang ,  Andrew Berlin ,  Qing Ma ,  Li-Peng Wang ,  Valluri Rao ,  Mineo Yamakawa

IPC分类号： G01N33/48

CPC分类号： G01N33/54373 ,  B82Y15/00 ,  B82Y30/00 ,  G01N27/126 ,  G01N29/022 ,  G01N29/036 ,  G01N29/2437 ,  G01N29/348 ,  G01N33/0031 ,  G01N2291/02466 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0426 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/144444 ,  Y10T436/25375

摘要： Systems and methods for detecting the presence of biomolecules in a sample using biosensors that incorporate resonators which have functionalized surfaces for reacting with target biomolecules. In one embodiment, a device includes a piezoelectric resonator having a functionalized surface configured to react with target molecules, thereby changing the mass and/or charge of the resonator which consequently changes the frequency response of the resonator. The resonator's frequency response after exposure to a sample is compared to a reference, such as the frequency response before exposure to the sample, a stored baseline frequency response or a control resonator's frequency response.

公开(公告)号：US07037382B2

公开(公告)日：2006-05-02

申请号：US10260224

申请日：2002-09-27

申请人： Thomas Davidson ,  Robert A. Phillips ,  Andres T. Hernandes ,  David B. Russell ,  Kevin Roche ,  Walter H. Zengerle, III ,  Andrew Berlin ,  Joshua P. Kinsley ,  Benjamin Daniel Sweet-Block ,  Darul Kisai

发明人： Thomas Davidson ,  Robert A. Phillips ,  Andres T. Hernandes ,  David B. Russell ,  Kevin Roche ,  Walter H. Zengerle, III ,  Andrew Berlin ,  Joshua P. Kinsley ,  Benjamin Daniel Sweet-Block ,  Darul Kisai

IPC分类号： B08B5/00

CPC分类号： B41J2/16552 ,  B29C41/12 ,  B29C64/165 ,  B29C64/35 ,  B29K2995/0021 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y40/00 ,  B41J2/01 ,  B41J2/16523 ,  B41J2/16535 ,  B41J2/16541

摘要： A three-dimensional printer uses inkjet-type printheads to rapidly prototype, or print, a three-dimensional model. A powder feeder includes a conveyor system and a metering system to deliver powder to a build area in measured quantities. The powder feeder also includes a vacuum system for loading powder into a feed reservoir or chamber. The vacuum system can also be used to cleanup excess powder. Other powder control features include powder gutters and magnetic powder plows. During printing, a cleaning system operates to remove powder from the printheads. In the event of a printhead or jet failure, the failure can be detected and corrective measures taken automatically. After printing, the model can be depowdered and infiltrated in an enclosure.

摘要翻译： 三维打印机使用喷墨式打印头来快速原型化或打印三维模型。 粉末进料器包括输送机系统和计量系统，以测量的量将粉末输送到建筑面积。 粉末进料器还包括用于将粉末装载到进料储存器或室中的真空系统。 真空系统也可用于清理多余的粉末。 其他粉末控制功能包括粉末槽和磁粉犁。 在打印期间，清洁系统操作以从打印头中去除粉末。 在打印头或喷射器故障的情况下，可以检测到故障并自动采取纠正措施。 打印后，模型可以被去除并渗透到外壳中。

公开(公告)号：US20050280185A1

公开(公告)日：2005-12-22

申请号：US11097987

申请日：2005-04-01

申请人： David Russell ,  Andres Hernandez ,  Joshua Kinsley ,  Andrew Berlin

发明人： David Russell ,  Andres Hernandez ,  Joshua Kinsley ,  Andrew Berlin

IPC分类号： B28B7/46 ,  B29C41/02 ,  B29C67/00

CPC分类号： B28B7/465 ,  B28B1/001 ,  B29C64/165 ,  B33Y10/00 ,  B33Y30/00

摘要： The invention relates to methods and apparatus for fabricating a three-dimensional object from a representation of the object stored in memory. The apparatus includes a stationary build table for receiving successive layers of a build material and at least one movable printhead disposed above the build table. The printhead deposits a binding material in a predetermined pattern on each successive layer of the build material to form the three-dimensional object.

摘要翻译： 本发明涉及从存储在存储器中的对象的表示来制造三维物体的方法和装置。 该装置包括用于接收构建材料的连续层的静止构建工作台和布置在构建工作台上方的至少一个可移动打印头。 打印头在构建材料的每个连续层上以预定图案沉积粘合材料以形成三维物体。

公开(公告)号：US20050224778A1

公开(公告)日：2005-10-13

申请号：US10819790

申请日：2004-04-07

申请人： Valery Dubin ,  Swaminathan Sivakumar ,  Andrew Berlin ,  Mark Bohr

发明人： Valery Dubin ,  Swaminathan Sivakumar ,  Andrew Berlin ,  Mark Bohr

IPC分类号： H01L21/768 ,  H01L29/788 ,  H01L51/00 ,  H01L51/30

CPC分类号： H01L21/76838 ,  B82Y10/00 ,  H01L21/76852 ,  H01L51/0021 ,  H01L51/0048 ,  H01L51/0052 ,  H01L2221/1094 ,  Y10S977/742 ,  Y10S977/936

摘要： A nano-electrode or nano-wire may be etched centrally to form a gap between nano-electrode portions. The portions may ultimately constitute a single electron transistor. The source and drain formed from the electrode portions are self-aligned with one another. Using spacer technology, the gap between the electrodes may be made very small.

摘要翻译： 可以将纳米电极或纳米线中心蚀刻以形成纳米电极部分之间的间隙。 这些部分可以最终构成单个电子晶体管。 由电极部分形成的源极和漏极彼此自对准。 使用间隔技术，可以使电极之间的间隙非常小。

公开(公告)号：US08883091B2

公开(公告)日：2014-11-11

申请号：US11450083

申请日：2006-06-09

申请人： Narayanan Sundararajan ,  Andrew Berlin

发明人： Narayanan Sundararajan ,  Andrew Berlin

IPC分类号： B01L3/00 ,  B01F13/00 ,  G01N15/14

CPC分类号： B01F13/0062 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2300/0874 ,  B01L2300/0887 ,  G01N15/1404 ,  G01N2015/1413 ,  Y10T436/25 ,  Y10T436/2575

摘要： A micro-fluidic device containing a micro-fluidic inlet channel to convey a process flow, a plurality of micro-fluidic focusing channels to each convey one of a plurality of focusing flows, a focusing manifold coupled with the inlet channel at an inlet port thereof and with the plurality of focusing channels at a plurality of focusing channel ports thereof to focus the process flow by contacting and hydrodynamically impacting at least three sides of the process flow with the focusing flows, and a micro-fluidic outlet channel coupled with the focusing manifold at an outlet channel port to convey the combined focused process flow and focusing flow from the focusing manifold.

摘要翻译： 一种微流体装置，其包含用于输送工艺流的微流体入口通道，多个微流体聚焦通道，每个输送多个聚焦流中的一个;聚焦歧管，其与入口通道在其入口端口处连接 并且多个聚焦通道在其多个聚焦通道端口处，以通过与聚焦流接触和流体动力地冲击处理流的至少三个侧面来聚焦工艺流程，以及与聚焦歧管耦合的微流体出口通道 在出口通道端口处传送来自聚焦歧管的组合的聚焦过程流和聚焦流。

公开(公告)号：US07686995B2

公开(公告)日：2010-03-30

申请号：US12192412

申请日：2008-08-15

申请人： Thomas Davidson ,  Robert A. Phillips ,  Andres T. Hernandez ,  David B. Russell ,  Kevin Roche ,  Walter H. Zengerle, III ,  Andrew Berlin ,  Joshua P. Kinsley ,  Benjamin Daniel Sweet-Block ,  Darul Kisai

发明人： Thomas Davidson ,  Robert A. Phillips ,  Andres T. Hernandez ,  David B. Russell ,  Kevin Roche ,  Walter H. Zengerle, III ,  Andrew Berlin ,  Joshua P. Kinsley ,  Benjamin Daniel Sweet-Block ,  Darul Kisai

IPC分类号： B29C31/04

CPC分类号： B29C41/36 ,  B22F3/008 ,  B29C41/12 ,  B29C64/165 ,  B29K2995/0021 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y40/00 ,  B41J2/155 ,  B41J2/16541 ,  B41J2/16552 ,  B41J2/17513 ,  B41J2/17556 ,  B41J2/17596 ,  B41J2002/14475 ,  B41J2202/20

摘要： A three-dimensional printer uses inkjet-type printheads to rapidly prototype, or print, a three-dimensional model. A powder feeder includes a conveyor system and a metering system to deliver powder to a build area in measured quantities. The powder feeder also includes a vacuum system for loading powder into a feed reservoir or chamber. The vacuum system can also be used to cleanup excess powder. Other powder control features include powder gutters and magnetic powder plows. During printing, a cleaning system operates to remove powder from the printheads. In the event of a printhead or jet failure, the failure can be detected and corrective measures taken automatically. After printing, the model can be depowdered and infiltrated in an enclosure.

摘要翻译： 三维打印机使用喷墨式打印头来快速原型化或打印三维模型。 粉末进料器包括输送机系统和计量系统，以测量的量将粉末输送到建筑面积。 粉末进料器还包括用于将粉末装载到进料储存器或室中的真空系统。 真空系统也可用于清理多余的粉末。 其他粉末控制功能包括粉末槽和磁粉犁。 在打印期间，清洁系统操作以从打印头中去除粉末。 在打印头或喷射器故障的情况下，可以检测到故障并自动采取纠正措施。 打印后，模型可以被去除并渗透到外壳中。

公开(公告)号：US07381529B2

公开(公告)日：2008-06-03

申请号：US10750515

申请日：2003-12-31

申请人： Mineo Yamakawa ,  Andrew Berlin

发明人： Mineo Yamakawa ,  Andrew Berlin

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q2565/601 ,  C12Q2563/155 ,  C12Q2525/179

摘要： A method for determining a nucleotide sequence of a nucleic acid is provided that includes contacting the nucleic acid with a series of labeled oligonucleotides for binding to the nucleic acid, wherein each labeled oligonucleotide includes a known nucleotide sequence and a molecular nanocode. The nanocode of an isolated labeled oligonucleotides that binds to the nucleic acid is then detected using SPM. Nanocodes of the present invention in certain aspects include detectable features beyond the arrangement of tags that encode information about the barcoded object, which assist in detecting the tags that encode information about the barcoded object. The detectable features include structures of a nanocode or associated with a nanocode, referred to herein as detectable feature tags, for error checking/error-correction, encryption, and data reduction/compression.

摘要翻译： 提供了用于确定核酸的核苷酸序列的方法，其包括使核酸与一系列用于结合核酸的标记的寡核苷酸接触，其中每个标记的寡核苷酸包括已知的核苷酸序列和分子纳代码。 然后使用SPM检测与核酸结合的分离的标记寡核苷酸的纳代码。 在某些方面，本发明的纳代码包括超过编码关于条形码对象的信息的标签布置的可检测特征，其有助于检测编码关于条形码对象的信息的标签。 可检测特征包括用于错误校验/纠错，加密和数据缩减/压缩的纳代码或与纳代码相关联的结构，这里称为可检测特征标签。