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    Peptide sequencing using mass spectrometry
    46.
    发明授权
    Peptide sequencing using mass spectrometry 失效
    使用质谱法进行肽测序

    公开(公告)号：US5470753A

    公开(公告)日：1995-11-28

    申请号：US68947

    申请日：1993-05-28

    申请人： Nikolai Sepetov ,  Olga Issakova ,  Viktor Krchnak ,  Michal Lebl

    发明人： Nikolai Sepetov ,  Olga Issakova ,  Viktor Krchnak ,  Michal Lebl

    IPC分类号： G01N33/68 ,  G01N33/00

    CPC分类号： G01N33/6848 ,  G01N33/6818 ,  Y10T436/24

    摘要： The present invention relates to methods for determining the amino acid composition, and more preferably the sequence, of a peptide using mass spectrometric techniques. The method is particularly useful for sequencing peptides isolated from natural sources or from libraries of peptides that have been prepared synthetically, and for peptides that are not amenable to Edman degradation sequencing. In one embodiment, the method for determining the amino acid composition or sequence of a peptide comprises determining the difference of the mass of the peptide from the mass of a deuterium-hydrogen exchanged peptide, and from this difference determining the number of exchangeable (labile) hydrogen atoms (protons). Candidate peptides having amino acid compositions or sequences that do not contain the observed number of exchangeable protons are eliminated. In another embodiment, synthesis of a portion of the peptides in a library of peptides is terminated after each coupling step, whereby a set of sequentially truncated fragments of each peptide is formed. Thus the sequence of the peptide can be determined by determining the difference in the molecular weight between each peptide or fragment thereof and the sequentially smaller fragment of the peptide.

    摘要翻译： 本发明涉及使用质谱技术测定肽的氨基酸组成，更优选地确定肽的序列的方法。 该方法对于从天然来源分离的肽或从已经合成制备的肽的文库以及不适合于Edman降解测序的肽测序肽特别有用。 在一个实施方案中，用于确定肽的氨基酸组成或序列的方法包括测定肽的质量与氘 - 氢交换的肽的质量的差异，以及从确定可交换（不稳定） 氢原子（质子）。 具有不含观察数量的可交换质子的氨基酸组成或序列的候选肽被消除。 在另一个实施方案中，在每个偶联步骤之后终止肽文库中肽的一部分的合成，由此形成每组肽的一系列顺序截短的片段。 因此，肽的序列可以通过确定每个肽或其片段与序列较小的肽片段之间的分子量的差异来确定。

    Electroosmotic pump with improved gas management
    48.
    发明授权
    Electroosmotic pump with improved gas management 有权
    电渗泵具有改进的气体管理

    公开(公告)号：US08252250B2

    公开(公告)日：2012-08-28

    申请号：US12626353

    申请日：2009-11-25

    申请人： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain

    发明人： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain

    IPC分类号： B01L3/00

    CPC分类号： F04B37/10 ,  F04B19/00 ,  Y10T436/2575

    摘要： An electroosmotic (EO) pump is provided that includes a housing having a pump cavity, a porous core medium and electrodes. The porous core medium is positioned within the pump cavity to form an exterior reservoir that extends at least partially about an exterior surface of the porous core medium. The porous core medium has an open inner chamber provided therein. The inner chamber represents an interior reservoir. The electrodes are positioned in the inner chamber and are positioned proximate the exterior surface. The electrodes induce flow of a fluid through the porous core medium between the interior and exterior reservoirs, wherein a gas is generated when the electrodes induce flow of the fluid. The housing has a fluid inlet to convey the fluid to one of the interior reservoir and the exterior reservoir. The housing has a fluid outlet to discharge the fluid from another of the interior reservoir and the exterior reservoir. The housing has a gas removal device to remove the gas from the pump cavity.

    摘要翻译： 提供一种电渗（EO）泵，其包括具有泵腔的壳体，多孔芯介质和电极。 多孔芯介质定位在泵腔内以形成至少部分地围绕多孔芯介质的外表面延伸的外部储存器。 多孔芯介质具有设置在其中的开放内室。 内室表示内部储存器。 电极定位在内室中并且位于外表面附近。 电极通过多孔芯介质在内部和外部储存器之间引导流体流动，其中当电极引起流体流动时产生气体。 壳体具有用于将流体输送到内部储存器和外部储存器中的一个的流体入口。 壳体具有流体出口，以从另一个内部储存器和外部储存器排出流体。 壳体具有用于从泵腔去除气体的气体去除装置。