公开(公告)号：US09280635B2

公开(公告)日：2016-03-08

申请号：US13820968

申请日：2011-10-25

申请人： Collin A. Rich

发明人： Collin A. Rich

IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N31/00 ,  G06F19/10 ,  G06F19/26 ,  G01N15/14 ,  G06F19/12 ,  G06M11/02

CPC分类号： G01N15/1434 ,  G01J3/0208 ,  G01J3/0229 ,  G01J3/18 ,  G01J3/36 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1429 ,  G06F19/10 ,  G06F19/12 ,  G06F19/26 ,  G06M11/02

摘要： Systems in a flow cytometer having an interrogation zone and illumination impinging the interrogation zone include: a lens subsystem including a collimating element that collimates light from the interrogation zone, a light dispersion element that disperses collimated light into a light spectrum, and a focusing lens that focuses the light spectrum onto an array of adjacent detection points; a detector array, including semiconductor detector devices, that collectively detects a full spectral range of input light signals, in which each detector device detects a subset spectral range of the full spectral range of light signals; and a user interface that enables a user to create a set of virtual detector channels by grouping detectors in the detector array, such that each virtual detector channel corresponds to a detector group and has a virtual detector channel range including the sum of subset spectral ranges of the detectors in the corresponding detector group.

摘要翻译： 具有询问区和照射询问区的照明的流式细胞仪中的系统包括：透镜子系统，包括准直来自询问区的光的准直元件，将准直光分散到光谱中的光分散元件和聚焦透镜， 将光谱聚焦到相邻检测点的阵列上; 检测器阵列，包括半导体检测器装置，其共同地检测输入光信号的全光谱范围，其中每个检测器装置检测光信号的全光谱范围的子集光谱范围; 以及用户界面，其使得用户能够通过对检测器阵列中的检测器进行分组来创建一组虚拟检测器通道，使得每个虚拟检测器通道对应于检测器组并且具有虚拟检测器通道范围，该虚拟检测器通道范围包括： 相应检测器组中的检测器。

公开(公告)号：US20130080082A1

公开(公告)日：2013-03-28

申请号：US13697739

申请日：2011-06-14

申请人： Grant C. Howes ,  Collin A. Rich

发明人： Grant C. Howes ,  Collin A. Rich

IPC分类号： G01F1/00 ,  G06F17/00 ,  G01F25/00

CPC分类号： G01F1/00 ,  G01F25/0007 ,  G01N15/14 ,  G01N35/00871 ,  G06F17/00 ,  G06F19/28

摘要： A system for creating a flow cytometer network includes: a flow cytometer with an interrogation zone and a fixed gain detection system that collects sample data from the interrogation zone; a flow cytometer data center that stores and manages sample- related data from the flow cytometer; and a network communication module that communicates sample-related data between the flow cytometer and the data center. The system may include a second flow cytometer and a second network communication module, where the first and second flow cytometers are calibrated to have substantially identical fixed gain settings. A method for creating a flow cytometer network includes: calibrating first and second flow cytometers with a calibration solution; collecting sample data with a fixed gain detection system; uploading data to a flow cytometer data center; retrieving data from the data center; and performing data analysis on the retrieved data.

摘要翻译： 用于产生流式细胞仪网络的系统包括：具有询问区的流式细胞仪和从询问区收集样本数据的固定增益检测系统; 流式细胞仪数据中心，用于存储和管理来自流式细胞仪的样品相关数据; 以及在流式细胞仪和数据中心之间传送样本相关数据的网络通信模块。 该系统可以包括第二流式细胞仪和第二网络通信模块，其中第一和第二流式细胞仪被校准以具有基本相同的固定增益设置。 创建流式细胞仪网络的方法包括：用校准溶液校准第一和第二流式细胞仪; 用固定增益检测系统收集样本数据; 将数据上传到流式细胞仪数据中心; 从数据中心检索数据; 并对所检索的数据执行数据分析。

公开(公告)号：US07981661B2

公开(公告)日：2011-07-19

申请号：US11736412

申请日：2007-04-17

申请人： Collin A. Rich

发明人： Collin A. Rich

IPC分类号： C12M1/36 ,  G01N33/48 ,  G01N27/22

CPC分类号： G01N15/1404 ,  G01N2015/1409 ,  Y10T436/115831

摘要： The fluidic system of the preferred embodiment includes a sheath pump to pump sheath fluid from a sheath container into an interrogation zone, a sheath volume measurement device to measure the fluid in the sheath container, a waste pump to pump the sheath fluid and a sample fluid as waste fluid from the interrogation zone into a waste container, and a waste volume measurement device to measure the fluid in the waste container. The system also includes a controller connected to the sheath pump, the waste pump, and the volume measurement devices. The sheath pump and/or the waste pump draw sample fluid from a sample container into the interrogation zone, which functions to provide a location for the fluidic system and an optical system of the flow cytometer to cooperatively facilitate the analysis of the sample fluid.

摘要翻译： 优选实施例的流体系统包括鞘套泵，以将护套流体从护套容器泵送到询问区域中;护套体积测量装置，用于测量护套容器中的流体;泵送护套流体的废泵和样品流体 作为从询问区到废物容器的废液，以及用于测量废物容器中的流体的废物体积测量装置。 该系统还包括连接到护套泵，废水泵和体积测量装置的控制器。 护套泵和/或废液泵将样品流体从样品容器抽入询问区，其功能是提供流体系统的位置和流式细胞计数器的光学系统，以便协调地促进样品流体的分析。

公开(公告)号：US20110058163A1

公开(公告)日：2011-03-10

申请号：US12939836

申请日：2010-11-04

申请人： Collin A. Rich

发明人： Collin A. Rich

IPC分类号： G01N1/10

CPC分类号： G01N15/1434 ,  G01N15/1459 ,  G02B27/141 ,  G02B27/145

摘要： The optical system of the preferred embodiments includes a first light source that creates a first beam of a first wavelength, a first collimating element that collimates the first beam, a second light source 102 that creates a second beam of a second wavelength, a second collimating element that collimates the second beam, a beam combining element that combines the collimated beams, and a focusing element that focuses the combined collimated beam to a single point.

摘要翻译： 优选实施例的光学系统包括产生第一波长的第一光束的第一光源，准直第一光束的第一准直元件，产生第二波长的第二光束的第二光源102，第二准直 对第二光束进行准直的元件，组合准直光束的光束组合元件以及将组合的准直光束聚焦到单个点的聚焦元件。

公开(公告)号：US20110008816A1

公开(公告)日：2011-01-13

申请号：US12792536

申请日：2010-06-02

申请人： Jack T. Ball ,  Grant C. Howes ,  Collin A. Rich

发明人： Jack T. Ball ,  Grant C. Howes ,  Collin A. Rich

IPC分类号： C12Q1/02 ,  C12M1/34

CPC分类号： G01N33/5094 ,  G01N15/1012 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1459 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1018 ,  G01N2015/1486 ,  G01N2333/70514 ,  Y10T436/10 ,  Y10T436/101666

摘要： A system and method for a flow cytometer system including a prepared sample fluid with reference beads; an interrogation zone that analyzes the prepared sample fluid; a peristaltic pump system that draws the sample fluid through the interrogation zone; and a processor that monitors a measured volume of sample fluid sampled by the peristaltic pump system and an expected sample volume based on data generated by the analysis of the sample fluid. A system and method is additionally described using an alternative volume sensing fluidic system.

摘要翻译： 一种用于流式细胞仪系统的系统和方法，其包括具有参比珠的制备的样品流体; 一个询问区，分析制备的样品液; 蠕动泵系统，通过询问区抽取样品液; 以及处理器，其基于通过对样品流体的分析产生的数据监测由蠕动泵系统采样的样品流体的测量体积和预期样品体积。 使用替代的体积感测流体系统另外描述系统和方法。

公开(公告)号：US20100302536A1

公开(公告)日：2010-12-02

申请号：US12476877

申请日：2009-06-02

申请人： Jack T. Ball ,  Collin A. Rich ,  Rogers E. Clare

发明人： Jack T. Ball ,  Collin A. Rich ,  Rogers E. Clare

IPC分类号： G01J3/30

CPC分类号： G01J3/4406

摘要： A system for a flow cytometer that collects data for a sample prepared with a plurality of fluorochromes that includes a fixed gain detection system that collects data for a plurality of fluorescence channels, fluorochrome compensation factors for a plurality of fluorochromes types, and a computer system that has an interface that gathers fluorochrome information of the sample and an analysis program that compensates for spectral spillover in the collected data. The fixed gain detection system preferably has a wide dynamic range. A fluorochrome compensation factor preferably remains constant for a fixed gain detection system. The analysis program preferably uses the fluorochrome compensation factors to compensate for spectral spillover.

摘要翻译： 一种用于流式细胞计数器的系统，其收集由多个荧光染料制备的样品的数据，所述荧光染料包括收集多个荧光通道的数据的固定增益检测系统，用于多个荧光染料类型的荧光染料补偿因子，以及计算机系统， 具有收集样品的荧光染料信息的界面和补偿收集的数据中的光谱外溢的分析程序。 固定增益检测系统优选具有宽的动态范围。 对于固定增益检测系统，荧光染料补偿因子优选保持恒定。 分析程序优选使用荧光染料补偿因子来补偿光谱溢出。

公开(公告)号：US07520300B2

公开(公告)日：2009-04-21

申请号：US11958278

申请日：2007-12-17

申请人： Collin A. Rich ,  Steven M. Martin

发明人： Collin A. Rich ,  Steven M. Martin

IPC分类号： F16L55/04

CPC分类号： F15B1/021 ,  F04B43/12 ,  F15B1/10 ,  F15B21/008 ,  F15B2201/205 ,  F15B2201/215 ,  F15B2201/3151 ,  G01N15/1404 ,  Y10T137/3118 ,  Y10T137/7792

摘要： A pulsation attenuator for a fluidic system with a fluidic pump. The pulsation attenuator includes a fluidic channel, a first fluidic device adapted to attenuate pulsations with a shallow rolloff slope, and a second fluidic device adapted to attenuate pulsations with a shallow rolloff slope. The first fluidic device and the second fluidic device are connected to the fluidic channel such that they cooperatively attenuate pulsations with a steep rolloff slope. Preferably, the first fluidic device includes a first fluidic resistor and a first fluidic capacitor, and the second fluidic device includes a second fluidic resistor and a second fluidic capacitor. Preferably, the pulsation attenuator is arranged, similar a second-order low-pass filter, in the following order: (1) first fluidic resistor, (2) first fluidic capacitor, (3) second fluidic resistor, and (4) second fluidic capacitor.

摘要翻译： 一种具有流体泵的流体系统的脉动衰减器。 脉动衰减器包括流体通道，适于衰减具有浅滚降斜率的脉动的第一流体装置，以及适于以浅滚降斜率衰减脉动的第二流体装置。 第一流体装置和第二流体装置连接到流体通道，使得它们以陡峭的滚降斜率协同地衰减脉动。 优选地，第一流体装置包括第一流体电阻器和第一流体电容器，并且第二流体装置包括第二流体电阻器和第二流体电容器。 优选地，按照以下顺序将脉动衰减器类似于二阶低通滤波器：（1）第一流体电阻器，（2）第一流体电容器，（3）第二流体电阻器，和（4）第二流体电阻器 电容器。

公开(公告)号：US20080228444A1

公开(公告)日：2008-09-18

申请号：US11466391

申请日：2006-08-22

申请人： David Olson ,  Collin A. Rich ,  Clement James Goebel

发明人： David Olson ,  Collin A. Rich ,  Clement James Goebel

IPC分类号： G06F15/00

CPC分类号： G01N15/1429 ,  G01N35/00712 ,  G01N35/0092

摘要： The flow cytometer user interface of the preferred embodiment comprises the steps of (1) running the sample and saving all collected data, (2) viewing the raw (or “unmodified”) data, (3) modifying the raw data (e.g., scaling and/or culling the raw data), (4) reviewing and saving the modified settings, and (5) exporting the saved data. Once the sample has been run and all collected data have been saved, the user can repeat the steps of modifying the raw data, saving the modified settings, and exporting the saved data as many times as necessary and/or desirable without the need to run an additional sample.

摘要翻译： 优选实施例的流式细胞仪用户界面包括以下步骤：（1）运行样本并保存所有收集的数据，（2）查看原始（或“未修改”）数据，（3）修改原始数据（例如缩放 和/或剔除原始数据），（4）查看和保存修改的设置，以及（5）导出保存的数据。 一旦样品运行并且所有收集的数据都被保存，用户可以重复修改原始数据，保存修改后的设置以及根据需要和/或需要导出保存的数据的步骤，而不需要运行 额外的样品。

公开(公告)号：US20080215297A1

公开(公告)日：2008-09-04

申请号：US11963384

申请日：2007-12-21

申请人： Clement James Goebel ,  Collin A. Rich

发明人： Clement James Goebel ,  Collin A. Rich

IPC分类号： G06F15/00 ,  G01N21/00

CPC分类号： G01N15/1459 ,  G01N2015/0092 ,  G01N2015/1477

摘要： The detection system of the first preferred embodiment includes a detector, having a wide dynamic range, that receives photonic inputs from the interrogation zone and produces an analog signal; and an analog-to-digital converter (ADC), having a high bit resolution, that is coupled to the detector and converts an analog signal to a digital signal. The digital signal includes an initial data set of the full dynamic range of the input signals from the flow cytometer sample. The method of extracting and analyzing data from a flow cytometer system of the first preferred embodiment preferably includes the steps of: collecting a full dynamic range of input signals from a flow cytometer sample; recognizing and annotating aggregate particle events; and storing an initial data set and an annotated data set of the full dynamic range of the input signals from the flow cytometer sample.

摘要翻译： 第一优选实施例的检测系统包括具有宽动态范围的检测器，其接收来自询问区的光子输入并产生模拟信号; 以及具有高比特分辨率的模数转换器（ADC），其耦合到检测器并将模拟信号转换为数字信号。 数字信号包括来自流式细胞仪样本的输入信号的完整动态范围的初始数据集。 从第一优选实施例的流式细胞仪系统中提取和分析数据的方法优选地包括以下步骤：从流式细胞仪样品收集输入信号的完整动态范围; 识别和注释聚集粒子事件; 并存储来自流式细胞仪样本的输入信号的完整动态范围的初始数据集和注释数据集。

公开(公告)号：US20080152542A1

公开(公告)日：2008-06-26

申请号：US11872676

申请日：2007-10-15

申请人： Jack T. Ball ,  Collin A. Rich

发明人： Jack T. Ball ,  Collin A. Rich

IPC分类号： G01N21/01 ,  G01N1/00 ,  F15B21/00 ,  G01N21/00

CPC分类号： G01N15/1404 ,  Y10T137/85978

摘要： The fluidic system 10 of the preferred embodiment includes a sheath pump 12 to pump sheath fluid 14 from a sheath container 16 through a sample port 34 into an interrogation zone 18 and a waste pump 20 to pump the sheath fluid 14 and a sample fluid 26 as waste fluid 22 from the interrogation zone 18 into a waste container 24, and a processor 30 to calculate a time window based on the flow rate of the sample fluid 26. Preferably the processor 30 also calculates a time window for the sample fluid to reach the interrogation zone 18 from the sample port 34 based on the flow rate of the sample fluid 26. The interrogation zone 18 functions to provide a location for the fluidic system 10 and an optical analysis system 32 of the flow cytometer to cooperatively facilitate the analysis of the sample fluid 26.

摘要翻译： 优选实施例的流体系统10包括护套泵12，其将护套流体14从鞘容器16通过样品端口34泵送到询问区域18和废水泵20，以将鞘液14和样品流体26泵送为 从询问区18到废物容器24的废液22以及基于样品流体26的流速计算时间窗的处理器30.优选地，处理器30还计算样品流体到达的时间窗口 询问区18基于样品流体26的流速从采样端口34询问。询问区18用于提供流体系统10的位置和流式细胞计数器的光学分析系统32，以便协调地促进对 样品流体26。