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公开(公告)号：US20070059716A1
公开(公告)日：2007-03-15
申请号：US11228454
申请日：2005-09-15
申请人： Ulysses Balis , Mehmet Toner , Ravi Kapur , John Walsh
发明人： Ulysses Balis , Mehmet Toner , Ravi Kapur , John Walsh
CPC分类号： C12Q1/6881 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2400/0487 , B01L2400/086 , B82Y15/00 , B82Y30/00 , C12Q2600/156 , G01N2015/1486
摘要： The invention relates to a method of identifying fetal abnormality from a maternal blood sample by capturing an image of a fetal nucleated red blood cell obtained from the maternal blood sample; inputting probe intensities for a plurality of nucleic acid probes that bind fetal nucleic acids of interest; analyzing the probe intensities; and generating a diagnostic output according to results of the analysis. In some embodiments, the probes are specific to a chromosome.
摘要翻译： 本发明涉及一种从母体血液样本中鉴别胎儿异常的方法，该方法是从母体血液样品中获取胎儿有核红细胞的图像， 输入结合感兴趣的胎儿核酸的多个核酸探针的探针强度; 分析探针强度; 并根据分析结果生成诊断输出。 在一些实施方案中，探针对染色体是特异性的。
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公开(公告)号：US20060134599A1
公开(公告)日：2006-06-22
申请号：US10529453
申请日：2003-09-29
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
摘要翻译： 本发明的特征在于用于从样品中分离细胞的方法（例如，从母体分离胎儿红细胞开始于将包含细胞的样品引入一个或多个微流体通道。在一个实施方案中，该装置包括至少两个处理步骤。 例如，将细胞混合物引入微流体通道中，该微流体通道选择性地允许期望类型的细胞通过，然后将富含所需类型的细胞群引入第二微流体通道，其允许所需的 细胞的选择基于混合物中细胞的性质，例如大小，形状，变形性，表面特征（例如，细胞表面受体或抗原和 膜渗透性）或细胞内特性（例如特定酶的表达）。
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公开(公告)号：US20050282293A1
公开(公告)日：2005-12-22
申请号：US11071270
申请日：2005-03-03
申请人： Maury Cosman , Ravi Kapur , Bruce Carvalho , Tom Barber , Ulysses Balis , Mehmet Toner , Lotien Huang , Darren Gray
发明人： Maury Cosman , Ravi Kapur , Bruce Carvalho , Tom Barber , Ulysses Balis , Mehmet Toner , Lotien Huang , Darren Gray
CPC分类号： G01N1/14 , B01L3/5027 , B01L3/50825 , B01L2200/026 , G01N1/38 , G01N35/1095 , Y10T436/2575
摘要： The invention features methods and devices for the delivery of a fluid medium containing analytes, e.g., particles, solutes, or solvents, to an analytical device. The systems are designed to minimize contact with potentially hazardous, fragile, or valuable samples. The systems allow for the dilution, mixing, and introduction of the fluid medium to an analytical device, followed by possible further analysis or sample manipulation.
摘要翻译： 本发明的特征在于将含有分析物例如颗粒，溶质或溶剂的流体介质输送到分析装置的方法和装置。 这些系统旨在最大程度地减少与潜在的危险，易碎或有价值的样品的接触。 该系统允许将流体介质稀释，混合和引入分析装置，随后进行可能的进一步分析或样品操作。
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44.发明授权公开(公告)号：US09174222B2
公开(公告)日：2015-11-03
申请号：US13232781
申请日：2011-09-14
申请人： Lotien Richard Huang , Thomas Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
发明人： Lotien Richard Huang , Thomas Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
CPC分类号： B03C1/30 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0472 , B01L2400/0487 , B01L2400/086 , B03C1/32 , B03C2201/18 , B33Y80/00 , C12M47/04 , C12M47/06 , G01N1/40 , G01N1/4077 , G01N33/5044 , G01N2035/00237 , Y10T137/0318 , Y10T137/8593 , Y10T436/25 , Y10T436/25375
摘要： The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.
摘要翻译： 本发明的特征在于确定性分离颗粒的装置和方法。 示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。 该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。 本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。 通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。
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公开(公告)号：US20130324418A1
公开(公告)日：2013-12-05
申请号：US13737730
申请日：2013-01-09
申请人： Martin Fuchs , Ravi Kapur , Mehmet Toner , Zihua Wang
发明人： Martin Fuchs , Ravi Kapur , Mehmet Toner , Zihua Wang
IPC分类号： G01N33/50
CPC分类号： C12Q1/6886 , C12Q2600/156 , G01N1/405 , G01N33/5005 , G01N33/5091 , G01N33/57484 , G01N2015/1006 , G01N2015/1087 , G01N2800/385
摘要： The present invention relates to methods for detecting, enriching, and analyzing rare cells that are present in the blood, e.g., epithelial cells. The invention further features methods of analyzing rare cell(s) to determine the presence of an abnormality, disease or condition in a subject by analyzing a cellular sample from the subject.
摘要翻译： 本发明涉及用于检测，富集和分析存在于血液中的稀有细胞例如上皮细胞的方法。 本发明进一步具有分析稀有细胞以通过分析来自受试者的细胞样品来确定受试者中异常，疾病或病症的存在的方法。
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公开(公告)号：US08372579B2
公开(公告)日：2013-02-12
申请号：US11800940
申请日：2007-05-08
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
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公开(公告)号：US20120006760A1
公开(公告)日：2012-01-12
申请号：US13086370
申请日：2011-04-13
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
CPC分类号： B01L3/502753 , B01L1/52 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
摘要翻译： 本发明的特征在于从样品中分离细胞的方法（例如，将胎儿红细胞与母体血液分离）。 该方法开始于将包含细胞的样品引入一个或多个微流体通道。 在一个实施例中，该装置包括至少两个处理步骤。 例如，将细胞混合物引入微流体通道中，该微流体通道选择性地允许期望类型的细胞通过，然后将富含所需类型的细胞群引入第二微流体通道，其允许所需的 细胞以产生进一步富集所需类型的细胞群体。 细胞的选择基于混合物中细胞的性质，例如，大小，形状，变形性，表面特征（例如，细胞表面受体或抗原和膜通透性）或细胞内特性（例如，特定的表达 酶）。
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公开(公告)号：US20100273675A1
公开(公告)日：2010-10-28
申请号：US12702614
申请日：2010-02-09
申请人： Ulysses J. Balis , Mehmet Toner , Ravi Kapur , John Walsh
发明人： Ulysses J. Balis , Mehmet Toner , Ravi Kapur , John Walsh
CPC分类号： C12Q1/6881 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2400/0487 , B01L2400/086 , B82Y15/00 , B82Y30/00 , C12Q2600/156 , G01N2015/1486
摘要： The invention relates to a method of identifying fetal abnormality from a maternal blood sample by capturing an image of a fetal nucleated red blood cell obtained from the maternal blood sample; inputting probe intensities for a plurality of nucleic acid probes that bind fetal nucleic acids of interest; analyzing the probe intensities; and generating a diagnostic output according to results of the analysis. In some embodiments, the probes are specific to a chromosome.
摘要翻译： 本发明涉及一种从母体血液样本中鉴别胎儿异常的方法，该方法是从母体血液样品中获取胎儿有核红细胞的图像， 输入结合感兴趣的胎儿核酸的多个核酸探针的探针强度; 分析探针强度; 并根据分析结果生成诊断输出。 在一些实施方案中，探针对染色体是特异性的。
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公开(公告)号：US20090181421A1
公开(公告)日：2009-07-16
申请号：US12172158
申请日：2008-07-11
申请人： Ravi Kapur , Diana Bianchi , Tom Barber , Mehmet Toner
发明人： Ravi Kapur , Diana Bianchi , Tom Barber , Mehmet Toner
IPC分类号： C12Q1/06
CPC分类号： G01N33/689 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0409 , B01L2400/0472 , B01L2400/086 , B82Y15/00 , B82Y30/00 , G01N1/40 , G01N1/4077 , G01N33/80 , G01N2800/368
摘要： The present invention relates to methods for diagnosing a condition in a fetus by enriching and enumerating circulating red blood cells with the possible combination of results from maternal serum marker screens.
摘要翻译： 本发明涉及通过用母体血清标记筛选结果的可能组合丰富和列举循环红细胞来诊断胎儿状况的方法。
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公开(公告)号：US20070264675A1
公开(公告)日：2007-11-15
申请号：US11800940
申请日：2007-05-08
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
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