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    Pharmaceutical Agent for Promoting the Functional Regeneration of Damaged Tissue
    42.
    发明申请
    Pharmaceutical Agent for Promoting the Functional Regeneration of Damaged Tissue 审中-公开
    促进损伤组织功能再生的药剂

    公开(公告)号：US20110097309A1

    公开(公告)日：2011-04-28

    申请号：US12990060

    申请日：2009-04-30

    申请人： Katsuto Tamai ,  Takehiko Yamazaki ,  Takenao Chino ,  Yasufumi Kaneda

    发明人： Katsuto Tamai ,  Takehiko Yamazaki ,  Takenao Chino ,  Yasufumi Kaneda

    IPC分类号： A61K35/12 ,  C12N5/02 ,  C12N15/63 ,  A61K38/00 ,  A61K31/7088 ,  C07K14/00 ,  A61P43/00

    CPC分类号： A61K31/7088 ,  A01K2227/105 ,  A01K2267/0393 ,  A61K38/1709 ,  C07K14/47 ,  C12N15/8509

    摘要： The present inventors assessed the possibility that bone marrow-derived cells are mobilized to the grafted skin from nonskin tissues and contribute to skin tissue regeneration during the engraftment of grafted skin on biological tissues. As a result, the present inventors for the first time in the world demonstrated that:(1) a large number of bone marrow-derived cells are mobilized to grafted skin;(2) mobilized bone marrow-derived cells differentiate into any of dermal fibroblasts, adipocytes, muscle cells, vascular endothelial cells, and epidermal keratinocytes in grafted skin, and thus mobilized bone marrow-derived cells include bone marrow-derived mesenchymal stem cells;(3) S100A8 and S100A9 released from necrotic tissues of grafted skin are responsible for mobilizing bone marrow-derived mesenchymal stem cells to the grafted skin from peripheral blood; and(4) purified S100A8 and S100A9 promote the migration of mesenchymal stem cells isolated/cultured from the bone marrow.

    摘要翻译： 本发明人评估了骨髓来源的细胞从非组织组织移植到嫁接皮肤并有助于在生物组织移植移植皮肤期间皮肤组织再生的可能性。 结果，本发明人在世界上第一次证明：（1）大量骨髓来源的细胞被移动到移植皮肤; （2）动员的骨髓来源的细胞分化成皮肤成纤维细胞，脂肪细胞，肌肉细胞，血管内皮细胞和表皮角化细胞在移植皮肤中的任何一种，从而动员骨髓来源的细胞包括骨髓间充质干细胞; （3）从移植皮肤坏死组织中释放的S100A8和S100A9负责将骨髓间充质干细胞从外周血移植到移植皮肤; 和（4）纯化的S100A8和S100A9促进从骨髓​​分离/培养的间充质干细胞的迁移。

    Virus envelope vector for gene transfer
    43.
    发明授权
    Virus envelope vector for gene transfer 有权
    用于基因转移的病毒包膜载体

    公开(公告)号：US07803621B2

    公开(公告)日：2010-09-28

    申请号：US11126770

    申请日：2005-05-10

    申请人： Yasufumi Kaneda

    发明人： Yasufumi Kaneda

    IPC分类号： A61K48/00 ,  C12N15/00

    CPC分类号： C12N7/00 ,  A61K48/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2710/16663 ,  C12N2760/18823 ,  C12N2760/18861 ,  C12N2760/18863

    摘要： A gene transfer vector is prepared by introducing an exogenous gene into an inactivated virus envelope, through a freezing and thawing treatment or mixing with a detergent. There are also provided a pharmaceutical composition for gene therapy containing this gene transfer vector, a kit containing this gene transfer vector, and a gene transfer method employing this gene transfer vector.

    摘要翻译： 通过冷冻和解冻处理或与洗涤剂混合将外源基因导入灭活的病毒包膜中制备基因转移载体。 还提供了含有该基因转移载体的基因治疗药物组合物，含该基因转移载体的试剂盒和使用该基因转移载体的基因转移方法。

    Decoy compositions for treating and preventing brain diseases and disorders
    45.
    发明申请
    Decoy compositions for treating and preventing brain diseases and disorders 审中-公开
    用于治疗和预防脑部疾病和疾病的诱饵组合物

    公开(公告)号：US20080207552A1

    公开(公告)日：2008-08-28

    申请号：US11982970

    申请日：2007-11-05

    申请人： Yoshiki Sawa ,  Ryuichi Morishita ,  Yasufumi Kaneda ,  Hikaru Matsuda ,  Toshiki Yoshimine

    发明人： Yoshiki Sawa ,  Ryuichi Morishita ,  Yasufumi Kaneda ,  Hikaru Matsuda ,  Toshiki Yoshimine

    IPC分类号： A61K31/7088 ,  A61P9/10

    CPC分类号： A61K9/0019 ,  A61K9/127 ,  A61K31/711 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/4705 ,  C12N15/1131 ,  C12N15/115 ,  C12N15/86 ,  C12N2310/13 ,  C12N2310/315 ,  C12N2760/18811

    摘要： The present invention provides introduction of NF-κB decoy oligodeoxynucleotide into rat cranial nerve through a carotid artery during global brain ischemia. Polymerase chain reaction demonstrated that one hour after global brain ischemia, transfected NF-κB decoy oligodeoxynucleotide effectively suppressed expression of tumor necrosis factor α, interleukin 1β and intracellular adhesion molecule 1 messenger RNAs. Terminal deoxynucleotidyl transferase-mediated deoxyuridine nick-end labeling staining and immunohistochemistry using microtubule-associated protein 2 demonstrated that transfected NF-κB decoy oligodeoxynucleotide significantly attenuated neuronal damage seven days after global brain ischemia. Therapeutic transfection of NF-κB decoy oligodeoxynucleotide during brain ischemia may be effective for attenuation of neuronal damage, suggesting a strategy for protecting the cerebrum from global ischemia.

    摘要翻译： 本发明提供了在全脑缺血期间通过颈动脉将NF-κB诱饵寡脱氧核苷酸引入大鼠颅神经中。 聚合酶链反应表明，全球脑缺血后1小时，转染的NF-κB诱饵寡脱氧核苷酸有效抑制肿瘤坏死因子α，白细胞介素1β和细胞内粘附分子1信使RNA的表达。 末端脱氧核苷酸转移酶介导的脱氧尿苷切口末端标记染色和使用微管相关蛋白2的免疫组织化学证实，转染的NF-κB诱饵寡脱氧核苷酸在全脑缺血后7天显着减弱神经元损伤。 在脑缺血期间，NF-κB诱饵寡脱氧核苷酸的治疗转染对于减少神经元损伤可能是有效的，这表明保护大脑免受全身缺血的策略。

    Surface acoustic wave device having reflectors with both open and shorted electrodes
    46.
    发明授权
    Surface acoustic wave device having reflectors with both open and shorted electrodes 有权
    表面声波装置具有带有开路和短路电极的反射器

    公开(公告)号：US07157991B2

    公开(公告)日：2007-01-02

    申请号：US10746255

    申请日：2003-12-29

    申请人： Osamu Kawachi ,  Takuya Abe ,  Yasufumi Kaneda ,  Motoyuki Tajima

    发明人： Osamu Kawachi ,  Takuya Abe ,  Yasufumi Kaneda ,  Motoyuki Tajima

    IPC分类号： H03H9/25 ,  H03H9/64

    CPC分类号： H03H9/02771

    摘要： A surface acoustic wave device for implementing an attenuation characteristic outside of the pass band and the narrow band characteristics, preferably for use as a filter. The surface acoustic wave device includes a piezoelectric substrate, interdigital transducers formed on the piezoelectric substrate, and first and second reflectors disposed on the sides of the interdigital transducers. Each of the first and second reflectors includes an open electrode block with a plurality of electrically independent electrode fingers, and a short electrode block with a plurality of electrode fingers of which both ends are electrically shorted.

    摘要翻译： 用于实现通带外的衰减特性和窄带特性的表面声波装置，优选用作滤波器。 表面声波装置包括压电基板，形成在压电基板上的叉指换能器，以及设置在叉指式换能器侧面的第一和第二反射器。 第一和第二反射器中的每一个包括具有多个电独立电极指的开放电极块，以及具有多个电极指的短电极块，其两端电气短路。

    Method of isolating nucleic acid having desired functional property and kit therefor
    47.
    发明申请
    Method of isolating nucleic acid having desired functional property and kit therefor 审中-公开
    分离具有所需功能特性的核酸的方法及其试剂盒

    公开(公告)号：US20060099597A1

    公开(公告)日：2006-05-11

    申请号：US10535907

    申请日：2003-11-20

    申请人： Yasufumi Kaneda ,  Tomoyuki Nishikawa

    发明人： Yasufumi Kaneda ,  Tomoyuki Nishikawa

    IPC分类号： C12Q1/68 ,  C12N5/08 ,  C12N15/86 ,  C12N15/88

    CPC分类号： C12N15/1086 ,  C12N15/1034

    摘要： The present invention provides a method for isolating a nucleic acid having an intended functional property conveniently and rapidly, as well as a kit for carrying out the method. Specifically, the present invention provides a method comprising the steps of: (A) transferring a nucleic acid into a plurality of first host cells and allowing the nucleic acid to transiently express therein; (B) selecting, from the first host cells into which the nucleic acid is transferred, a cell which a nucleic acid having an intended functional property has been transferred; (C) preparing a purified nucleic acid from the selected cell; and (D) selecting a purified nucleic acid having an intended functional property, as well as a kit to carry out the method.

    摘要翻译： 本发明提供方便，快速地分离具有预期功能特性的核酸的方法以及用于实施该方法的试剂盒。 具体而言，本发明提供了一种方法，其包括以下步骤：（A）将核酸转移到多个第一宿主细胞中并允许核酸在其中瞬时表达; （B）从转移有核酸的第一宿主细胞中选择具有预期功能性的核酸已被转移的细胞; （C）从所选择的细胞制备纯化的核酸; 和（D）选择具有预期功能特性的纯化核酸，以及用于实施该方法的试剂盒。

    DNA vaccine and methods for its use
    48.
    发明授权
    DNA vaccine and methods for its use 失效
    DNA疫苗及其使用方法

    公开(公告)号：US06472375B1

    公开(公告)日：2002-10-29

    申请号：US09144837

    申请日：1998-08-31

    申请人： Dave S. B. Hoon ,  Yasufumi Kaneda

    发明人： Dave S. B. Hoon ,  Yasufumi Kaneda

    IPC分类号： A61K4800

    CPC分类号： B82Y5/00 ,  A61K9/1271 ,  A61K39/0011 ,  A61K48/00

    摘要： DNA cancer vaccines and methods for their use are described. The vaccines are comprised of viral liposomes comprising nucleic acid, preferably DNA, encoding a tumor-associated antigen. The viral liposomes may be formed by the fusion of HVJ reagents with nonviral reagents. The vaccine may be administered subcutaneously, intradermally, intramuscularly or into an organ. The vaccine may be administered to induce a host normal cell to express the tumor associated antigen.

    摘要翻译： 描述了DNA癌症疫苗及其使用方法。 疫苗由包含编码肿瘤相关抗原的核酸，优选DNA的病毒脂质体组成。 病毒脂质体可以通过HVJ试剂与非病毒试剂的融合形成。 疫苗可以皮下，皮内，肌肉内或器官内给药。 可以施用疫苗以诱导宿主正常细胞以表达肿瘤相关抗原。

    Mutant Paramyxovirus and Method for Production Thereof
    50.
    发明申请
    Mutant Paramyxovirus and Method for Production Thereof 有权
    突变型副粘病毒及其生产方法

    公开(公告)号：US20090269850A1

    公开(公告)日：2009-10-29

    申请号：US12085355

    申请日：2006-11-24

    申请人： Yasufumi Kaneda ,  Katsuto Tamai ,  Kotaro Saga ,  Masako Kawachi

    发明人： Yasufumi Kaneda ,  Katsuto Tamai ,  Kotaro Saga ,  Masako Kawachi

    IPC分类号： C12N15/86 ,  C12N7/01 ,  C12N5/10 ,  C07K14/00 ,  C07K16/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K2319/30 ,  C07K2319/33 ,  C12N15/1131 ,  C12N15/86 ,  C12N2310/14 ,  C12N2760/18821 ,  C12N2760/18822 ,  C12N2760/18843 ,  C12N2760/18845 ,  C12N2810/859

    摘要： The present invention provides a modified paramyxovirus containing a reduced amount of receptor-binding protein compared with the wild type; a method of preparing a modified paramyxovirus, comprising the following steps: (1) a step for introducing a nucleic acid that suppresses the expression of a receptor-binding protein of a paramyxovirus into an animal cell, (2) a step for infecting the paramyxovirus to the cell, and (3) a step for isolating paramyxovirus particles replicated in the cell; and a modified paramyxovirus prepared by the method of preparation mentioned above.The present invention also provides a chimera protein wherein a fusion protein of a virus has been joined or bound to a peptide that binds specifically to a cell surface marker; a nucleic acid that encodes the chimera protein; an animal cell capable of expressing the chimera protein on the cell surface thereof; a modified paramyxovirus expressing the chimera protein on the virus particle surface thereof; and a method of preparing a tissue targeting paramyxovirus, comprising: (1) a step for supplying a nucleic acid that encodes a chimera protein wherein a fusion protein of a virus has been joined or bound to a peptide that binds specifically to a cell surface marker of the target cells, (2) a step for introducing the nucleic acid supplied in (1) into an animal cell in an expressible state, and expressing the same, (3) a step for infecting a paramyxovirus to the cell, and (4) a step for isolating paramyxovirus particles replicated in the cell.

    摘要翻译： 本发明提供了与野生型相比含有减少量的受体结合蛋白的修饰的副粘病毒; 一种制备修饰的副粘病毒的方法，包括以下步骤：（1）将抑制副粘病毒的受体结合蛋白的表达的核酸引入动物细胞的步骤，（2）感染副粘病毒的步骤 （3）用于分离复制在细胞中的副粘病毒颗粒的步骤; 和通过上述制备方法制备的修饰的副粘病毒。 本发明还提供一种嵌合体蛋白，其中病毒的融合蛋白已经与特异性结合于细胞表面标记的肽结合或结合; 编码嵌合蛋白的核酸; 能够在其细胞表面上表达嵌合体蛋白质的动物细胞; 在病毒颗粒表面上表达嵌合体蛋白质的修饰的副粘病毒; 以及制备组织靶向副粘病毒的方法，包括：（1）提供编码嵌合体蛋白的核酸的步骤，其中病毒的融合蛋白已经结合或与特异性结合于细胞表面标记的肽结合 的靶细胞，（2）将（1）中提供的核酸以可表达状态引入动物细胞中并表达其的步骤，（3）感染细胞的副粘病毒的步骤，（4 ）分离在细胞中复制的副粘病毒颗粒的步骤。