公开(公告)号：US07432113B2

公开(公告)日：2008-10-07

申请号：US11134749

申请日：2005-05-19

申请人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

发明人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

IPC分类号： G01N33/553

CPC分类号： G01N21/658

摘要： The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.

公开(公告)号：US20080241569A1

公开(公告)日：2008-10-02

申请号：US11694293

申请日：2007-03-30

申请人： Haoyu Qin ,  Jingwu Zhang ,  Claire E. Dentinger ,  Lei Sun ,  Xing Su

发明人： Haoyu Qin ,  Jingwu Zhang ,  Claire E. Dentinger ,  Lei Sun ,  Xing Su

IPC分类号： B32B5/16

CPC分类号： G01N21/658 ,  B82Y15/00 ,  B82Y30/00 ,  Y10T428/12028 ,  Y10T428/2991

摘要： Embodiments of the present invention provide methods for coating nanoparticles with polymeric coatings and nanoparticles that are coated with polymeric coatings. The polymeric coatings typically comprise two or more layers wherein the first layer has a charge that is opposite to that of the second layer. In further embodiments, the nanoparticles that can act as labels or reporters are coated with polymeric coatings. Optionally, these reporter or label nanoparticles may be Raman-active, such that they provide a distinctive Raman signature upon excitation with electromagnetic radiation.

摘要翻译： 本发明的实施方案提供了用聚合物涂层和纳米颗粒涂覆聚合物涂层的方法。 聚合物涂层通常包含两层或更多层，其中第一层具有与第二层相反的电荷。 在另外的实施方案中，可以用作标记或报告物的纳米颗粒涂覆有聚合物涂层。 任选地，这些报告物或标记纳米颗粒可以是拉曼活性的，使得它们在用电磁辐射激发时提供独特的拉曼特征。

公开(公告)号：US20080160623A1

公开(公告)日：2008-07-03

申请号：US11645814

申请日：2006-12-27

申请人： Xing Su

发明人： Xing Su

IPC分类号： G01F1/00 ,  G01F1/56 ,  G01N27/06

CPC分类号： G01F1/708 ,  G01F1/56 ,  G01F1/7086 ,  Y10T436/143333

摘要： An embodiment of the invention relates to a device and method for measuring small fluid volume and detecting an analyte in a sample. The device has a channel having a plurality of sensors in a wall of the channel, wherein the sensors are located along a length of the channel and are configured to detect a leading edge and a trailing edge of a volume of a fluid flowing though the channel. The sensors could be electrodes. The device could further have a binding surface on a portion of the wall of the channel and a detector in the vicinity of the wall of the channel, wherein the binding surface has molecules that bind to an analyte and the detector could be adapted to detect binding of the analyte to the binding surface.

摘要翻译： 本发明的一个实施例涉及用于测量小流体体积并检测样品中分析物的装置和方法。 该装置具有在通道的壁中具有多个传感器的通道，其中传感器沿着通道的长度定位，并且构造成检测流过通道的流体体积的前缘和后缘 。 传感器可以是电极。 该装置还可以在通道的壁的一部分上具有结合表面，并且在通道的壁附近具有检测器，其中结合表面具有结合分析物的分子，并且检测器可适于检测结合 的分析物到结合表面。

公开(公告)号：US20080074661A1

公开(公告)日：2008-03-27

申请号：US11524489

申请日：2006-09-21

申请人： Jingwu Zhang ,  Sarah Ngola ,  Xing Su

发明人： Jingwu Zhang ,  Sarah Ngola ,  Xing Su

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01N30/74 ,  B01J2220/54 ,  G01N21/658 ,  G01N27/44721 ,  G01N30/52 ,  G01N30/6082 ,  G01N30/7233 ,  G01N30/78 ,  G01N2030/525 ,  G01N2030/746

摘要： Devices and methods for separating and detecting analytes in a sample. The separation can be accomplished utilizing capillary electrophoresis (CE) or high-performance liquid chromatography (HPLC). The detection can be accomplished by surface enhanced Raman scattering.

摘要翻译： 用于分离和检测样品中分析物的设备和方法。 可以使用毛细管电泳（CE）或高效液相色谱（HPLC）来实现分离。 检测可以通过表面增强拉曼散射来实现。

公开(公告)号：US20080070796A1

公开(公告)日：2008-03-20

申请号：US11874858

申请日：2007-10-18

申请人： Hajime Matsuzaki ,  Xing Su ,  Sean Walsh ,  Giulia Kennedy ,  Rui Mei

发明人： Hajime Matsuzaki ,  Xing Su ,  Sean Walsh ,  Giulia Kennedy ,  Rui Mei

IPC分类号： C40B30/04 ,  C12Q1/68 ,  C40B40/08

CPC分类号： C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/683 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q2600/156

摘要： The presently claimed invention provides for novel methods and kits for analyzing a collection of target sequences in a nucleic acid sample. A sample is amplified under conditions that enrich for a subset of fragments that includes a collection of target sequences. The invention further provides for analysis of the above sample by hybridization to an array, which may be specifically designed to interrogate the collection of target sequences for particular characteristics, such as, for example, the presence or absence of one or more polymorphisms.

摘要翻译： 本发明提供了用于分析核酸样品中靶序列的集合的新颖方法和试剂盒。 在富集包括靶序列集合的片段子集的条件下扩增样品。 本发明进一步提供了通过与阵列杂交来分析上述样品，其可以被特别设计成询问用于特定特征的靶序列的收集，例如存在或不存在一个或多个多态性。

公开(公告)号：US20080032308A1

公开(公告)日：2008-02-07

申请号：US11845698

申请日：2007-08-27

申请人： Xing Su ,  Narayanan Suadararajan ,  Tae-Woong Koo

发明人： Xing Su ,  Narayanan Suadararajan ,  Tae-Woong Koo

IPC分类号： C12Q1/68

CPC分类号： G01N33/54353 ,  B01L3/5027 ,  C12N15/1006 ,  C12Q1/6869 ,  C12Q2565/629 ,  C12Q2521/319 ,  C12Q2565/518

摘要： Devices and methods for isolating, detecting, and positioning single polymeric molecules without the need for expensive equipment are provided. The disclosed devices and methods allow for a molecule to be quickly and efficiently transported to a specific sub-micron area. Such devices are useful, for instance, for performing analyses in which the sequence of a polymer of interest is determined.

摘要翻译： 提供了用于隔离，检测和定位单个聚合物分子的装置和方法，而不需要昂贵的设备。 所公开的装置和方法允许将分子快速有效地运送到特定的亚微米区域。 这样的装置例如用于进行其中确定感兴趣聚合物序列的分析是有用的。

公开(公告)号：US20070128615A1

公开(公告)日：2007-06-07

申请号：US11414681

申请日：2006-04-28

申请人： Xing Su

发明人： Xing Su

IPC分类号： C12Q1/68 ,  C12M3/00

CPC分类号： C12Q1/6818 ,  C12Q1/6874 ,  C12Q2565/632

摘要： Provided herein, is a nucleic acid sequencing method based on detection of Raman signatures of oligonucleotide probes. Raman signatures of individually captured nucleic acid probes, optionally labeled by a Raman label or a positively charged enhancer, are detected. The sequences of captured probes are used to identify the nucleotide sequences of captured probes and complementary target nucleic acids, which are then aligned and used to obtain nucleic acid sequence information. In another embodiment, a method is provided for determining a nucleotide occurrence at a target nucleotide position of a target nucleic acid, that utilizes binding of the target nucleic acid to a labeled oligonucleotide probe that binds to the target nucleic acid, wherein the labeled oligonucleotide probe includes a first label and a second label, the first label being capable of affecting an optical property of the second label.

公开(公告)号：US20070054288A1

公开(公告)日：2007-03-08

申请号：US11430612

申请日：2006-05-08

申请人： Xing Su ,  Tae-Woong Koo ,  Andrew Berlin ,  Lei Sun ,  Narayanan Sundararajan ,  Mineo Yamakawa

发明人： Xing Su ,  Tae-Woong Koo ,  Andrew Berlin ,  Lei Sun ,  Narayanan Sundararajan ,  Mineo Yamakawa

IPC分类号： C12Q1/68 ,  C12M1/34 ,  G06K7/10

CPC分类号： B82Y10/00 ,  B82Y5/00 ,  C12Q1/6816 ,  G06K19/06028 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2565/1025

摘要： The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind to a target, such as proteins, nucleic acids and other biomolecules or aggregates. Different barcodes may be distinguished by the type and location of the tags. In other embodiments, barcodes may be produced by hybridization of one or more tagged oligonucleotides to a template, comprising a container section and a probe section. The tagged oligonucleotides may be designed as modular code sections, to form different barcodes specific for different targets. In alternative embodiments, barcodes may be prepared by polymerization of monomeric units. Bound barcodes may be detected by various imaging modalities, such as, surface plasmon resonance, fluorescent or Raman spectroscopy.

摘要翻译： 本公开涉及用于生产和/或使用分子条形码的方法。 在本发明的某些实施方案中，条形码包含可包含一个或多个分支结构的聚合物主链。 标签可以附加到骨干和/或分支结构。 条形码还可以包含可以与靶标结合的探针，例如蛋白质，核酸和其他生物分子或聚集体。 可以通过标签的类型和位置区分不同的条形码。 在其它实施方案中，条形码可以通过将一个或多个标记的寡核苷酸与包含容器部分和探针部分的模板杂交来产生。 标记的寡核苷酸可以被设计为模块代码部分，以形成针对不同靶标的不同条形码。 在替代实施例中，条形码可以通过单体单元的聚合来制备。 可以通过各种成像方式，例如表面等离子体共振，荧光或拉曼光谱来检测结合条形码。

公开(公告)号：US20070048746A1

公开(公告)日：2007-03-01

申请号：US11216112

申请日：2005-09-01

申请人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

发明人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/34

CPC分类号： G01N33/53 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/54366 ,  G01N33/5438

摘要： Method and device to collect multiplex data simultaneously in analyte detection and analyze the data by experimentally trained software (machine-learning) is disclosed. Various ways (magnetic particles and microcoils) are disclosed to collect multiple reporter (tag) signals. Multiplex detection can increase the biomolecule analysis efficiency by using small sample size and saving assay reagents and time. Machine learning and data analysis schemes are also disclosed. Multiple affinity binding partners, each labeled by a unique reporter, are contacted with a sample and a green spectrum is taken to detect multiple reporter signals. The spectrum is deconvoluted by experimentally trained software to identify multiple analytes.

摘要翻译： 公开了通过实验训练的软件（机器学习）在分析物检测中同时收集多重数据并分析数据的方法和装置。 公开了各种方式（磁性颗粒和微型线圈）以收集多个报道（标签）信号。 多重检测可以通过使用小样本量和节省测定试剂和时间来增加生物分子分析效率。 还披露了机器学习和数据分析方案。 将多个亲和力结合配偶体（各自由唯一报道分子标记）与样品接触，并采用绿色光谱来检测多个报道信号。 通过实验训练的软件对光谱进行去卷积，以识别多个分析物。

公开(公告)号：US20060147941A1

公开(公告)日：2006-07-06

申请号：US11026857

申请日：2004-12-30

申请人： Xing Su

发明人： Xing Su

IPC分类号： C12Q1/68

CPC分类号： B82Y30/00 ,  C12Q1/6874 ,  C12Q2565/632

摘要： SERS technology is used for high throughput screening of biological analytes and samples. For polynucleotide sequencing, sets of oligonucleotide probes are labeled with composite organic-inorganic nanoparticles (COIN) that produce distinguishable SERS signals when excited by a laser. Detection of a hybridization complex containing members of two such COIN-labeled probe sets will reveal a 12 nucleotide sequence segment of the target polynucleotide. Also provided are surface-modified arrays and chips with multiple arrays to which sets of probe-conjugated COIN or other reporter substrates are immobilized. Analytes are detected by contacting a sample, such as a bodily fluid, with the array-anchored probes. Captured analytes are tagged with an additional target-specific Raman-active tag. Two or more Raman signatures emanating from the detection complexes reveal the identity of the captured analytes.

摘要翻译： SERS技术用于生物分析物和样品的高通量筛选。 对于多核苷酸测序，寡核苷酸探针组用复合有机 - 无机纳米粒子（COIN）标记，当由激光激发时产生可区分的SERS信号。 含有两个这样的COIN标记的探针组成员的杂交复合物的检测将显示靶多核苷酸的12个核苷酸序列片段。 还提供了具有多个阵列的表面改性阵列和芯片，固定有探针缀合的COIN或其它报告基质的组。 通过使样品（例如体液）与阵列锚定的探针接触来检测分析物。 捕获的分析物用额外的目标特异性拉曼活性标签进行标记。 从检测复合物中发出的两个或多个拉曼标记揭示了捕获的分析物的身份。