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    Methods and kits for indirect labeling of nucleic acids
    41.
    发明授权
    Methods and kits for indirect labeling of nucleic acids 失效
    间接标记核酸的方法和试剂盒

    公开(公告)号：US06235483B1

    公开(公告)日：2001-05-22

    申请号：US09495152

    申请日：2000-01-31

    Applicant: Paul K. Wolber ,  Karen W. Shannon

    Inventor： Paul K. Wolber ,  Karen W. Shannon

    IPC: C12Q168

    CPC classification number: C12Q1/6806 ,  C12Q1/6837 ,  C12Q2563/179 ,  C12Q2531/143 ,  C12Q2525/185

    Abstract: Methods and kits for labeling nucleic acids are provided. In the subject methods, an oligonucleotide tagged nucleic acid comprising an oligonucleotide tag is first generated. The oligonucleotide tagged nucleic acid is then contacted under hybridization conditions with a labeled oligonucleotide complementary to the oligonucleotide tag, yielding a labeled nucleic acid. The kits of the subject invention at least include a primer for use in enzymatically generating an oligonucleotide tagged target nucleic acid, where the primer generally at least includes an oligo dT region and the oligonucleotide tag, and a labeled oligonucleotide complementary to the oligonucleotide tag. The subject methods and kits find use in a variety of applications, and are particularly suited for use in gene expression analysis applications.

    Abstract translation: 提供了用于标记核酸的方法和试剂盒。 在本方法中，首先生成包含寡核苷酸标签的寡核苷酸标记的核酸。 然后将寡核苷酸标记的核酸在杂交条件下与与寡核苷酸标签互补的标记的寡核苷酸接触，产生标记的核酸。 本发明的试剂盒至少包括用于酶促产生寡核苷酸标记的靶核酸的引物，其中引物通常至少包含寡聚dT区和寡核苷酸标签，以及与寡核苷酸标签互补的标记的寡核苷酸。 主题方法和试剂盒可用于各种应用，特别适用于基因表达分析应用。

    Oligonucleotides, methods and kits for amplifying and detecting a nucleic acid of cytomegalovirus (CMV) using nucleic acid sequence .beta.2.7
    42.
    发明授权
    Oligonucleotides, methods and kits for amplifying and detecting a nucleic acid of cytomegalovirus (CMV) using nucleic acid sequence .beta.2.7 失效
    使用核酸序列β2.7扩增和检测巨细胞病毒（CMV）的核酸的寡核苷酸，方法和试剂盒

    公开(公告)号：US5853981A

    公开(公告)日：1998-12-29

    申请号：US839306

    申请日：1997-04-18

    Applicant: Motohiro Kondo ,  Toshiya Aono ,  Masaya Segawa ,  Koichi Yamanishi ,  Kazuhiro Kondo ,  Keiko Taya

    Inventor： Motohiro Kondo ,  Toshiya Aono ,  Masaya Segawa ,  Koichi Yamanishi ,  Kazuhiro Kondo ,  Keiko Taya

    IPC: C12N15/09 ,  C12Q1/68 ,  C12Q1/70

    CPC classification number: C12Q1/701 ,  C12Q2531/143

    Abstract: The present invention relates to two primers for amplifying a cytomegalovirus (CMV) nucleic acid suitable for a nucleic acid sequence-based amplification (NASBA) using a DNA-dependent RNA polymerase, a first primer containing a promoter sequence and a nucleic acid sequence consisting of at least fifteen continuous bases selected from the nucleic acid sequence of SEQ ID NO:1; a second primer containing a nucleic acid sequence consisting of at least fifteen continuous bases selected from the nucleic acid sequence of SEQ ID NO:2, NO:3 or NO:4; a detecting probe and/or a capturing probe containing a nucleic acid sequence consisting of at least continuous fifteen bases selected from the nucleic acid sequence(s) of SEQ ID NO:5, NO:6 and/or NO:7 wherein said sequence is modified if necessary; a reagent kit for detecting a CMV containing the above-mentioned primers and probes; and a nucleic acid sequence-based amplification (NASBA) using said primers. The advantages of the present invention are that CMV can be detected in an easy, rapid and specific manner with a high sensitivity.

    Abstract translation: 本发明涉及用于扩增适用于使用DNA依赖性RNA聚合酶的基于核酸序列的扩增（NASBA）的巨细胞病毒（CMV）核酸的两个引物，含有启动子序列的第一引物和包含启动子序列的核酸序列 至少15个选自SEQ ID NO：1的核酸序列的连续碱基; 含有选自SEQ ID NO：2，NO：3或NO：4的核酸序列的至少十五个连续碱基的核酸序列的第二引物; 检测探针和/或捕获探针，其含有至少连续十五个碱基的核酸序列，所述核酸序列选自SEQ ID NO：5，NO：6和/或NO：7的核酸序列，其中所述序列是 必要时修改; 用于检测含有上述引物和探针的CMV的试剂盒; 和使用所述引物的基于核酸序列的扩增（NASBA）。 本发明的优点是可以以高灵敏度以容易，快速和具体的方式检测CMV。

    DUAL POLARITY ANALYSIS OF NUCLEIC ACIDS
    46.
    发明申请
    DUAL POLARITY ANALYSIS OF NUCLEIC ACIDS 审中-公开

    公开(公告)号：US20170362641A1

    公开(公告)日：2017-12-21

    申请号：US15689120

    申请日：2017-08-29

    Applicant: Enzo Life Sciences, Inc.

    Inventor： Elazar RABBANI ,  James J. Donegan

    IPC: C12Q1/68

    CPC classification number: C12Q1/6809 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q2565/525 ,  C12Q2565/501 ,  C12Q2531/143

    Abstract: This invention provides methods for characterizing the amounts of nucleic acids, including plus/minus determinations, the use of different constructs, the use of a library and a reference library. Expression may also be compared in two or more samples using the methods of this invention. Also provided are heterophasic arrays comprising labeled positive copies of nucleic acids hybridized to the array and labeled negative copies of nucleic acids hybridized to the array, in which the labeled positive copies are separately quantifiable from the labeled negative copies.

    LOCALISED RCA-BASED AMPLIFICATION METHOD USING A PADLOCK-PROBE
    48.
    发明申请
    LOCALISED RCA-BASED AMPLIFICATION METHOD USING A PADLOCK-PROBE 审中-公开

    公开(公告)号：US20160289750A1

    公开(公告)日：2016-10-06

    申请号：US15036394

    申请日：2014-11-14

    Applicant: OLINK AB

    Inventor： Ulf LANDEGREN ,  Lei CHEN

    IPC: C12Q1/68

    CPC classification number: C12Q1/6844 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6853 ,  C12Q1/6862 ,  C12Q1/6876 ,  C12Q2531/125 ,  C12Q2563/179 ,  C12Q2531/143 ,  C12Q2537/125 ,  C12Q2531/119 ,  C12Q2537/143 ,  C12Q2539/113 ,  C12Q2525/161 ,  C12Q2565/102 ,  C12Q2525/307 ,  C12Q2563/131

    Abstract: The present invention provides a method for performing a localised RCA reaction comprising at least two rounds of RCA, wherein the product of a second RCA reaction is attached, and hence localised, to a product of a first RCA reaction, said method comprising: (a) providing a concatemeric first RCA product comprising repeated monomers; (b) directly or indirectly hybridising to monomers of said first RCA product a circularisable oligonucleotide comprising target-complementary 3′ and 5′ end regions such that the 3′ and 5′ ends of said oligonucleotide hybridise in juxtaposition for ligation directly or indirectly to each other, wherein the target is a sequence in a monomer of said first RCA product or an intermediate molecule hybridised thereto, and wherein the target-complementary end regions of said circularisable oligonucleotide are 6 to 16 nucleotides in length; (c) directly or indirectly ligating the ends of said circularisable oligonucleotide to circularise the oligonucleotide, thereby to provide a template for a second RCA reaction, wherein when said ends are indirectly ligated (i) either a gap oligonucleotide is provided which hybridises to the monomers of the first RCA product in between the 3′ and 5′ ends of the circularisable oligonucleotide such that it may be ligated to the respective ends, or the hybridised 3′ end of the circularisable oligonucleotide is extended by a polymerase such that the extended 3′ end may be ligated to the hybridised 5′ end, and (ii) the total length of the region of the second RCA template directly or indirectly hybridised to the monomers is no longer than 32 nucleotides in length; and (d) performing a second RCA reaction using said second RCA template of (c) and a primer for said second RCA, to form a second RCA product, wherein in said second RCA reaction the second RCA template remains attached to the first RCA product, and thereby the second RCA product is attached to the first RCA product.

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