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    Methods and kits for isolating biological target materials using silica magnetic particles
    43.
    发明申请
    Methods and kits for isolating biological target materials using silica magnetic particles 审中-公开
    使用二氧化硅磁性颗粒分离生物靶材的方法和试剂盒

    公开(公告)号：US20020086326A1

    公开(公告)日：2002-07-04

    申请号：US10041890

    申请日：2002-01-07

    Applicant: Promega Corporation

    Inventor： Craig E. Smith ,  Charles K. York

    IPC: C12Q001/68 ,  C12N015/00

    CPC classification number: C12N15/1013 ,  C12Q1/6806 ,  Y10S977/838 ,  Y10S977/904 ,  Y10S977/916 ,  Y10S977/92 ,  Y10S977/924 ,  Y10S977/96

    Abstract: The present invention provides methods for isolating biological target materials, particularly nucleic acids, such as DNA or RNA or hybrid molecules of DNA and RNA, from other substances in a medium using silica magnetic particles. The methods of the present invention involve forming a complex of the silica magnetic particles and the biological target material in a mixture of the medium and particles, separating the complex from the mixture using external magnetic force, and eluting the biological target material from the complex. The preferred embodiments of magnetic silica particles used in the methods and kits of the present invention are capable of forming a complex with at least 2 nullg of biological target material per milligram of particle, and of releasing at least 60% of the material from the complex in the elution step of the method. The methods of the present invention produce isolated biological target material which is substantially free of contaminants, such as metals or macromolecular substances, which can interfere with further processing or analysis, if present.

    Abstract translation: 本发明提供了使用二氧化硅磁性颗粒从介质中的其他物质分离生物靶材料，特别是DNA或RNA或DNA和RNA的混合分子的方法。 本发明的方法包括在介质和颗粒的混合物中形成二氧化硅磁性颗粒和生物目标材料的复合物，使用外部磁力从混合物中分离复合物，并从复合物中洗脱生物目标物质。 在本发明的方法和试剂盒中使用的磁性二氧化硅颗粒的优选实施方案能够与每毫克颗粒至少2个生物靶材料形成复合物，并且从复合物中释放至少60％的材料 在该方法的洗脱步骤中。 本发明的方法产生基本上不含污染物（例如金属或大分子物质）的分离的生物靶材料，如果存在可能会干扰进一步的加工或分析。

    Magnetic resonance method and apparatus for detecting an atomic structure of a sample along a surface thereof
    46.
    发明授权
    Magnetic resonance method and apparatus for detecting an atomic structure of a sample along a surface thereof 失效
    用于检测样品沿其表面的原子结构的磁共振方法和装置

    公开(公告)号：US5619139A

    公开(公告)日：1997-04-08

    申请号：US393121

    申请日：1995-02-21

    Applicant: Karoly Holczer ,  Dieter Schmalbein ,  Peter Hoefer

    Inventor： Karoly Holczer ,  Dieter Schmalbein ,  Peter Hoefer

    IPC: G01B7/34 ,  G01Q30/02 ,  G01Q60/08 ,  G01Q60/52 ,  G01R33/56 ,  G01R33/60 ,  G01R33/20

    CPC classification number: G01Q60/08 ,  B82Y35/00 ,  G01Q60/52 ,  G01R33/60 ,  G01R33/5604 ,  Y10S977/838 ,  Y10S977/84 ,  Y10S977/863 ,  Y10S977/865 ,  Y10S977/88 ,  Y10S977/96

    Abstract: A method and an apparatus are disclosed for detecting an atomic structure of a sample along a surface thereof. The method comprises arranging the sample in a constant magnetic field (B.sub.0) of predetermined field strength and high homogeneity and irradiating a high-frequency magnetic field (B.sub.1) of a predetermined frequency on the sample, wherein the fields (B.sub.0) and (B.sub.1) are oriented perpendicularly to each other. The method further comprises providing a force-sensitive sensor having a paramagnetic tip comprising a paramagnetic material. The sensor is placed in close vicinity to the sample such that the paramagnetic tip is in atomic interaction with the sample surface which means that the distance between the tip and the surface is in the order of between 1 and 10 .ANG.. The predetermined field strength and the predetermined frequency are set such that electron paramagnetic resonance (EPR) is excited within the tip paramagnetic material. The paramagnetic tip is then displaced parallel to the sample surface for mapping predetermined points on the sample surface. During displacing the tip the force exerted on the tip by a local inhomogeneous magnetic field (B.sub.loc) caused by atomic magnetic moments (m.sub.e,k) of the sample is measured.

    Abstract translation: 公开了一种用于检测样品沿其表面的原子结构的方法和装置。 该方法包括将样品置于具有预定场强和高均匀性的恒定磁场（B0）中，并且对样品照射预定频率的高频磁场（B1），其中场（B0）和（B1） 彼此垂直取向。 该方法还包括提供具有包括顺磁材料的顺磁性尖端的力敏感传感器。 将传感器放置在样品附近，使得顺磁性尖端与样品表面原子相互作用，这意味着尖端和表面之间的距离在1和10埃之间。 将预定的场强和预定频率设定为使得电子顺磁共振（EPR）在尖端顺磁性材料内被激发。 然后顺磁性尖端平行于样品表面移位，用于映射样品表面上的预定点。 在移动尖端期间，测量由样品的原子磁矩（me，k）引起的局部不均匀磁场（Bloc）施加在尖端上的力。

    Process of preparing particles for NMR imaging
    47.
    发明授权
    Process of preparing particles for NMR imaging 失效
    制备核磁共振成像颗粒的方法

    公开(公告)号：US5587199A

    公开(公告)日：1996-12-24

    申请号：US421329

    申请日：1995-04-13

    Applicant: Herve Tournier ,  Roland Hyacinthe ,  Michel Schneider

    Inventor： Herve Tournier ,  Roland Hyacinthe ,  Michel Schneider

    IPC: A61K9/127 ,  A61B5/055 ,  A61K20060101 ,  A61K49/00 ,  A61K49/18 ,  B05D7/00 ,  B05D3/12 ,  B06B1/00 ,  B32B9/00

    CPC classification number: A61K49/1806 ,  A61K49/1839 ,  Y10S977/838 ,  Y10S977/904 ,  Y10S977/905 ,  Y10S977/927 ,  Y10S977/96 ,  Y10T428/2991 ,  Y10T428/2998 ,  Y10T436/24

    Abstract: Magnetite particles suitable for injection into the blood stream of patients having enhanced resistance against agglomeration and uptake by the RES of the liver and spleen. The particles essentially consist of an iron oxide core and a phosphoric acid mono alkyl or alkenyl ester or glycerophospholipid/surfactant three dimensional shell surrounding the core. The core and the monoester or a micellar glycerophospholipid form an urchin-like structure which is further interlaced or intertwined with a non-ionic surfactant to produce a protective three dimensional shell which renders particles almost undetectable by the macrophages. Particles prepared according to the invention are kept in the blood circulation for long periods and represent excellent long lasting blood pool agents. Key components in the shell are (a) a polybasic mineral-organic species such as glycero phosphatidic acid in micellar form and (b) a block copolymer having successive hydrophilic and hydrophobic segments.

    Abstract translation: 适合于注射到患有肝脏和脾脏的RES的聚集和摄取的抗性的患者血液中的磁铁矿颗粒。 颗粒基本上由氧化铁芯和磷酸单烷基或链烯基酯或包含核心的甘油磷脂/表面活性剂三维壳组成。 核心和单酯或胶束甘油磷脂形成海胆样结构，其进一步与非离子表面活性剂交织或交织在一起，以产生保护性三维壳，其使颗粒几乎不被巨噬细胞检测到。 根据本发明制备的颗粒长时间保持在血液循环中并且代表优异的持久的血液池剂。 壳中的关键组分是（a）多元矿物有机物质，例如胶束形式的甘油磷脂酸，和（b）具有连续亲水和疏水链段的嵌段共聚物。

    Particles for NMR imaging
    48.
    发明授权
    Particles for NMR imaging 失效
    核磁共振成像用颗粒

    公开(公告)号：US5464696A

    公开(公告)日：1995-11-07

    申请号：US096414

    申请日：1993-07-26

    Applicant: Herve Tournier ,  Roland Hyacinthe ,  Michel Schneider

    Inventor： Herve Tournier ,  Roland Hyacinthe ,  Michel Schneider

    IPC: A61K9/127 ,  A61B5/055 ,  A61K20060101 ,  A61K49/00 ,  A61K49/18 ,  B23B5/16

    CPC classification number: A61K49/1806 ,  A61K49/1839 ,  Y10S977/838 ,  Y10S977/904 ,  Y10S977/905 ,  Y10S977/927 ,  Y10S977/96 ,  Y10T428/2991 ,  Y10T428/2998 ,  Y10T436/24

    Abstract: Magnetite particles suitable for injection into the blood stream of patients having enhanced resistance against agglomeration and uptake by the RES of the liver and spleen. The particles essentially consist of an iron oxide core and a phosphoric acid mono alkyl or alkenyl ester or glycerophospholipid/surfactant three dimensional shell surrounding the core. The core and the monoester or a micellar glycerophospholipid form an urchin-like structure which is further interlaced or intertwined with a non-ionic surfactant to produce a protective three dimensional shell which renders particles almost undetectable by the macrophages. Particles prepared according to the invention are kept in the blood circulation for long periods and represent excellent long lasting blood pool agents. Key components in the shell are (a) a polybasic mineral-organic species such as glycero phosphatidic acid in micellar form and (b) a block copolymer having successive hydrophilic and hydrophobic segments.

    Abstract translation: 适合于注射到患有肝脏和脾脏的RES的聚集和摄取的抗性的患者血液中的磁铁矿颗粒。 颗粒基本上由氧化铁芯和磷酸单烷基或链烯基酯或包含核心的甘油磷脂/表面活性剂三维壳组成。 核心和单酯或胶束甘油磷脂形成海胆样结构，其进一步与非离子表面活性剂交织或交织在一起，以产生保护性三维壳，其使颗粒几乎不被巨噬细胞检测到。 根据本发明制备的颗粒长时间保持在血液循环中并且代表优异的持久的血液池剂。 壳中的关键组分是（a）多元矿物有机物质，例如胶束形式的甘油磷脂酸，和（b）具有连续亲水和疏水链段的嵌段共聚物。

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