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公开(公告)号:US5837115A
公开(公告)日:1998-11-17
申请号:US474688
申请日:1995-06-07
IPC分类号: B01J19/00 , B01L3/00 , C12M1/42 , C12M3/08 , G01N15/02 , G01N15/14 , G01N27/447 , G01N30/60 , G01N30/92 , B01D57/02 , G01N27/453
CPC分类号: G01N27/44791 , B01J19/0093 , B01L3/502753 , B01L3/502761 , C12M47/04 , B01L2200/0647 , B01L2200/0652 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/0877 , B01L2400/0421 , B01L2400/086 , G01N15/0255 , G01N2015/149 , G01N30/6095 , G01N30/92
摘要: A sorting apparatus and method for fractionating and simultaneously viewing individual microstructures, such as free cells, viruses, macromolecules, or minute particles in a fluid medium. The sorting apparatus is composed of a substrate having a receptacle located therein, the receptacle having sidewalls and a floor. An array of obstacles is positioned within the receptacle with the obstacles upstanding from the floor of the receptacle. A transparent cover overlies the array of obstacles to cover the receptacle and afford visual observation of migration of the microstructures exclusively through the array of obstacles. Electrodes may be positioned within the receptacle to generate an electric field in the fluid medium in the receptacle in order to induce the migration of the microstructures. Migration of the microstructures may also occur, for example, by a hydrodynamic field, an optical field, a magnetic field, or a gravity field applied to the receptacle. The obstacles of the array of obstacles may be of various shapes such as round posts, rectangular bunkers, or v-shaped or cup-shaped structures. The arrays of obstacles are formed of a predetermined and reproducible pattern, and can be reused. Methods for manufacturing and using the apparatus are also claimed.
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公开(公告)号:US5773576A
公开(公告)日:1998-06-30
申请号:US612882
申请日:1996-05-15
摘要: Support materials for thin-layer chromatography characterized by a marker code applied using laser light.
摘要翻译: PCT No.PCT / EP94 / 02871 Sec。 371日期:1996年5月15日 102(e)日期1996年5月15日PCT 1994年8月30日PCT PCT。 公开号WO95 / 07459 日期1995年3月16日用于使用激光施加标记代码的薄层色谱的支撑材料。
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公开(公告)号:US5736188A
公开(公告)日:1998-04-07
申请号:US512358
申请日:1995-08-08
申请人: Susan Alcock , Stephen White , Anthony Turner , Steven Setford , Ibtisam Tothill , Jon Dicks , Sarah Stephens , Jennifer Hall , Phillip Warner
发明人: Susan Alcock , Stephen White , Anthony Turner , Steven Setford , Ibtisam Tothill , Jon Dicks , Sarah Stephens , Jennifer Hall , Phillip Warner
IPC分类号: G01N27/327 , B01L3/00 , B81B1/00 , G01N27/416 , G01N30/32 , G01N30/92 , A01N1/02 , B05D1/32 , B05D5/00
CPC分类号: G01N30/92 , B01L3/5023 , B01L2300/0645 , B01L2300/0816 , B01L2300/0864 , B01L2300/0887 , B01L2400/0406 , B01L3/5027 , G01N2030/324
摘要: A backing sheet (101) is provided with a pattern of pathways (131,132,133) of (e.g.) silica or cellulose by a printing process (e.g., screen printing). There may be multiple pathways leading from an eluant application region (117) to a detection zone (116) and thence to a waste reservoir (118). Different pathways may have different fluid traversal times because they differ in length and/or material (e.g., nitrocellulose for slow traversal and fibrous cellulose for rapid traversal by an aqueous liquid). Thus analyte and reagents deposited at depots (112,113) on different pathways are sequentially delivered to the detection zone. Reagents may be applied by printing. The detection zone may have an electrode assembly (105), also applied by printing, for detecting the effects of analyte.
摘要翻译: 背衬片(101)通过印刷方法(例如,丝网印刷)具有(例如)二氧化硅或纤维素的途径(131,132,133)的图案。 可能存在从洗脱剂施用区域(117)引导到检测区域(116)并且然后到达废物储存器(118)的多个途径。 不同的途径可能具有不同的流体穿越时间,因为它们的长度和/或材料不同(例如,慢速穿过的硝化纤维素和水性液体快速穿透的纤维状纤维素)。 因此,将沉积在不同路径上的贮库(112,113)处的分析物和试剂依次递送到检测区。 可通过印刷应用试剂。 检测区可以具有也通过印刷施加的用于检测分析物的影响的电极组件(105)。
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公开(公告)号:US5320811A
公开(公告)日:1994-06-14
申请号:US729960
申请日:1991-07-15
CPC分类号: G01N30/92 , B01L3/0293
摘要: An apparatus and method for directly applying a liquid sample matrix containing analytes and possible endogenous compounds to selected areas of a thin layer chromatographic sheet. The apparatus comprises a manifold made from an upper and lower filter block which are fastened together and supported on a support member. The thin layer chromatographic sheet is held between the upper and lower filter block. Samples are applied to the thin layer chromatographic sheet through aligned through-bores in the upper and lower filter block by connecting a syringe to the through-bores of the upper filter block. The method involves initially wetting areas of the thin layer chromatographic sheet where the sample is subsequently applied. Analytes retained on the thin layer chromatographic sheet are subsequently analyzed and may be further extracted from the thin layer chromatographic sheet for analysis.
摘要翻译: 一种用于将含有分析物和可能的内源化合物的液体样品基质直接施加到薄层色谱图的选定区域的装置和方法。 该装置包括由上下过滤块组成的歧管,该歧管紧固在一起并支撑在支撑构件上。 薄层色谱片保持在上下过滤块之间。 将样品通过将注射器连接到上部过滤器块的通孔上而通过上部和下部过滤器块中的对准的通孔施加到薄层色谱片。 该方法首先润湿薄层色谱片的区域,其中随后应用样品。 随后分析保留在薄层色谱板上的分析物,并可从薄层色谱板中进一步提取分析物进行分析。
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公开(公告)号:US4945205A
公开(公告)日:1990-07-31
申请号:US215198
申请日:1988-07-05
申请人: David Litman , Robert Zuk , Gerald Rowley
发明人: David Litman , Robert Zuk , Gerald Rowley
CPC分类号: G01N30/92 , G01N30/90 , Y10S435/97
摘要: A device is disclosed for use in a chromatographic system wherein a component of a mixture is partitioned between a liquid phase and an immobile phase. The device comprises at least one strip of a bibulous material. In the chromatographic system the component traverses at least a portion of the strip. The strip generally has a longitudinal edge substantially corresponding to the direction of traverse of the component. The longitudinal edge has the characteristic of substantially the same rate of traversal by the component along this edge when compared to the rate of traversal of the component along the body of the strip. The strips are prepared from a sheet of a bibulous material by non-defomative or non-compressive cutting of the sheet. The preferred cutting means is a laser beam.
摘要翻译: 公开了用于色谱系统的装置,其中混合物的组分在液相和固定相之间分配。 该装置包括至少一条吸水材料条。 在色谱系统中,组件穿过条带的至少一部分。 条带通常具有基本上对应于部件的横向方向的纵向边缘。 当与沿着带的主体的部件的穿过速率相比时,纵向边缘具有与沿着该边缘的部件完全相同的穿过速率的特征。 通过片材的非消除或非压缩切割从带状吸收材料的片材制备条带。 优选的切割装置是激光束。
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公开(公告)号:US4810381A
公开(公告)日:1989-03-07
申请号:US137811
申请日:1987-12-28
IPC分类号: B01J20/281 , B01D15/08 , B01J20/28 , B01J20/285 , G01N30/88 , G01N30/92 , B01D39/06 , B01D39/16 , B01D39/20
CPC分类号: B01J20/28028 , B01J20/28033 , Y10S435/803 , Y10T428/249954 , Y10T428/249986
摘要: A composite chromatographic article comprising:(a) a polytetrafluoroethylene fibril matrix, and(b) non-swellable sorptive particles enmeshed in said matrix, the ration of non-swellable sorptive particles to polytetrafluoroethylene being in the range of 19:1 to 4:1 by weight, said composite article having a net surface energy in the range of 20 to 300 milliNewtons per meter.
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公开(公告)号:US4793921A
公开(公告)日:1988-12-27
申请号:US130572
申请日:1987-12-09
申请人: Heinz E. Hauck , Willi Jost
发明人: Heinz E. Hauck , Willi Jost
CPC分类号: B01J20/281 , B01J20/103 , B01J20/28057 , B01J20/286 , B01J20/287 , B01J20/3204 , B01J20/3257 , B01J20/3263 , B01J20/3285 , G01N30/90 , B01J2220/54
摘要: New separation materials for thin layer chromatography are based on carrier materials coated with an adsorbent silica gel layer which has been surface-modified by a silanizing agent after the coating step. The degree of covering of the modifying reagent can be adjusted in a defined an controllable manner, e.g., from 0.15 to 0.8 .mu.mol/m.sup.2 in the case of reversed phase materials and 1.2 to 2.5 .mu.mol/m.sup.2 in the case of hydrophilic materials. A process for the preparation of these separation materials is also provided.
摘要翻译: 用于薄层色谱的新的分离材料基于涂覆有在涂布步骤之后被硅烷化剂表面改性的吸附剂硅胶层的载体材料。 在反相材料的情况下,修饰试剂的覆盖程度可以以可限定的方式调节,例如为0.15〜0.8μmol/ m 2,亲水性材料为1.2〜2.5μmol/ m2。 还提供了制备这些分离材料的方法。
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公开(公告)号:US4676898A
公开(公告)日:1987-06-30
申请号:US869295
申请日:1986-06-02
申请人: Vinit Saxena
发明人: Vinit Saxena
CPC分类号: G01N30/38 , B01D15/14 , B01D15/18 , B01D15/1885 , G01N2030/386 , G01N2030/522 , G01N30/6004 , G01N30/92 , G01N30/94
摘要: A chromatographic column is disclosed in which flow through the separating medium bed takes place horizontally. The column is packed with small, rigid, high performance separation medium. The fluid sample containing several components flows horizontally through the stationary phase or medium and is separated at least partially into discrete components. Means for providing even horizontal flow distribution of the sample fluid and for collection of the discrete components of the sample fluid are included. The stationary phase consists of a packed bed of high performance, small size particles of either (a) absorbants such as silica, alumina etc; (b) packing for reverse phase ion-exchange, affinity, hydrophobic, chiral or ion chromatography; (c) hydroxylapatite; or packing used for desalting. The column as a whole may be fabricated to be disposable or may be constructed to have a more simplified but durable housing.
摘要翻译: 公开了一种色谱柱,其中通过分离介质床的流动水平地发生。 该柱包装有小型,刚性,高性能的分离介质。 含有几个组分的流体样品水平流过固定相或介质,并且至少部分地分离成离散组分。 包括用于提供样品流体的均匀水平分布和用于收集样品流体的分立组分的装置。 固定相由(a)吸收剂如二氧化硅,氧化铝等的高性能,小粒径的填充床组成; (b)用于反相离子交换,亲和力,疏水性,手性或离子色谱的包装; (c)羟基磷灰石; 或用于脱盐的包装。 作为整体的塔可以被制造为一次性的或者可以构造成具有更简化但耐用的外壳。
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公开(公告)号:US4587020A
公开(公告)日:1986-05-06
申请号:US641552
申请日:1984-08-16
申请人: Yuzo Nakagawa , Kouji Iwatani , Tetsuro Kadono
发明人: Yuzo Nakagawa , Kouji Iwatani , Tetsuro Kadono
CPC分类号: G01N30/92
摘要: A chromatographic plate comprising a support having a plurality of linear grooves engraved on the rear is disclosed. Each of said grooves have a uniform depth of a half or less as large as the thickness of the support and serves to guide a cutter. After the chromatographic development, the support may easily be cloven along the grooves into strips together with an adsorbent layer carried by the front of the support to give a sample holder for mass spectrometry.
摘要翻译: 公开了一种色谱板,其包括具有刻在后面的多个线性槽的支撑体。 每个所述凹槽具有与支撑件的厚度一样大的一半或更小的均匀深度并且用于引导切割器。 在色谱显影之后,支撑体可以容易地沿着沟槽与支撑体前面携带的吸附层一起形成条带,以得到用于质谱的样品保持器。
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公开(公告)号:US4189304A
公开(公告)日:1980-02-19
申请号:US955452
申请日:1978-10-27
CPC分类号: G01N33/523 , G01N30/48 , G01N33/558 , B01J2220/54 , G01N30/92
摘要: A test device and method for detecting myoglobin in the presence of hemoglobin in a fluid test sample. The test device comprises a chromatographic medium incorporating serum, haptoglobin and/or antiserum which binds or retards hemoglobin while permitting myoglobin to traverse the length of the test device. A test sample of body fluid is placed in contact with an appropriate portion of the chromatographic medium of said test device; hemoglobin in the body fluid is retained or retarded by the serum, haptoglobin and/or antiserum; and myoglobin in the sample traverses the length of the medium and contacts reagent means at a predetermined location thereon, resulting in a detectable response thereat.
摘要翻译: 用于在流体测试样品中血红蛋白存在下检测肌红蛋白的测试装置和方法。 测试装置包括含有血清,触珠蛋白和/或抗血清的色谱介质,其结合或延迟血红蛋白,同时允许肌红蛋白穿过测试装置的长度。 将体液的测试样品放置成与所述测试装置的色谱介质的适当部分接触; 体液中的血红蛋白被血清,触珠蛋白和/或抗血清保留或延迟; 样品中的肌红蛋白穿过培养基的长度并在其上的预定位置接触试剂装置,从而产生可检测的响应。
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