公开(公告)号：US20190153479A1

公开(公告)日：2019-05-23

申请号：US16200485

申请日：2018-11-26

申请人： Danisco US Inc. ,  The Goodyear Tire & Robber Company

发明人： Zachary Q. BECK ,  Michael C. MILLER ,  Caroline M. PERES ,  Yuliya A. PRIMAK ,  Jeff P. PUCCI ,  Derek H. WELLS

IPC分类号： C12P5/00 ,  C12P5/02 ,  C12N9/04 ,  C12N15/70 ,  C12P7/42 ,  C12P7/04 ,  C12P7/02 ,  C12N9/88 ,  C12N9/18 ,  C12N9/12 ,  C12N9/10 ,  C12N9/02

摘要： The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).

公开(公告)号：US20190127765A1

公开(公告)日：2019-05-02

申请号：US16245143

申请日：2019-01-10

申请人： DANISCO US INC

发明人： Andrei Miasnikov ,  Jeffrey Wayne Munos

IPC分类号： C12P7/10 ,  C12N9/10 ,  C12P7/06 ,  C12N9/02 ,  C12N9/88 ,  C12N15/52

CPC分类号： C12P7/10 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/52 ,  C12P7/06 ,  C12Y102/0101 ,  C12Y203/01008 ,  C12Y401/02009 ,  Y02E50/16 ,  Y02E50/17

摘要： A recombinant yeast cell, fermentation compositions, and methods of use thereof are provided. The recombinant yeast cell includes at least one heterologous nucleic acid encoding one or more polypeptide having phosphoketolase activity; phosphotransacetylase activity; and/or acetylating acetaldehyde dehydrogenase activity, wherein the cell does not include a heterologous modified xylose reductase gene, and wherein the cell is capable of increased biochemical end product production in a fermentation process when compared to a parent yeast cell.

公开(公告)号：US10240168B2

公开(公告)日：2019-03-26

申请号：US15126376

申请日：2015-03-19

申请人： Danisco US Inc.

发明人： Miasnikov Andrei ,  Jeffrey W. Munos

IPC分类号： C12P7/06 ,  C12P7/10 ,  C12N9/88 ,  C12N9/02 ,  C12N9/10 ,  C12N15/52

摘要： A recombinant yeast cell, fermentation compositions, and methods of use thereof are provided. The recombinant yeast cell includes at least one heterologous nucleic acid encoding one or more polypeptide having phosphoketolase activity; phosphotransacetylase activity; and/or acetylating acetaldehyde dehydrogenase activity, wherein the cell does not include a heterologous modified xylose reductase gene, and wherein the cell is capable of increased biochemical end product production in a fermentation process when compared to a parent yeast cell.

公开(公告)号：US20190085341A1

公开(公告)日：2019-03-21

申请号：US16081082

申请日：2017-03-06

申请人： DANISCO US INC.

发明人： Cristina Bongiorni ,  Marc Kolkman ,  Chris Leeflang ,  Virgil Arthur Rhodius ,  Anita Van Kimmenade

IPC分类号： C12N15/75 ,  C12N9/54 ,  C12N9/28

摘要： The instant disclosure is generally related to novel engineered (hybrid) promoters. In certain embodiments, the instant disclosure is directed to one or more nucleic acid compositions comprising engineered promoters operably linked to nucleic acids encoding proteins of interest. Thus, the disclosure set forth herein described methods and compositions for the production of proteins of interest using one or more novel engineered (hybrid) promoters of the disclosure.

公开(公告)号：US20190010473A1

公开(公告)日：2019-01-10

申请号：US16064518

申请日：2016-12-20

申请人： Danisco US Inc.

发明人： Bart C. Koops ,  Paula Johanna Maria Teunissen ,  Marco Van Brussel-Zwijnen ,  Martijn Scheffers ,  Kees-Jan Guijt ,  Zhengzheng Zou ,  Zhongmei Tang ,  Zhen Qian ,  Jing Ge ,  Zhenghong Zhang

IPC分类号： C12N9/30 ,  C12P19/14 ,  C12P7/06 ,  C12N9/34 ,  C12P19/02

摘要： Described are methods and compositions relating to granular starch-converting glucoamylases and α-amylases. The enzymes can be used to perform enzymatic starch hydrolysis of granular starch at or below the gelatinization temperature of insoluble granular starch.

公开(公告)号：US20190002854A1

公开(公告)日：2019-01-03

申请号：US16064577

申请日：2016-12-20

申请人： Bart C. KOOPS ,  Paula Johanna Maria TEUNISSEN ,  Marco VAN BRUSSEL-ZWIJNEN ,  Martijn SCHEFFERS ,  Kees-Jan GUIJT ,  Zhengzheng ZOU ,  Zhongmei TANG ,  Zhen QIAN ,  Jing GE ,  Zhenghong ZHANG ,  Danisco US Inc.

发明人： Bart C. Koops ,  Paula Johanna Maria Teunissen ,  Marco Van Brussel-Zwijnen ,  Martijn Scheffers ,  Kees-Jan Guijt ,  Zhengzheng Zou ,  Zhongmei Tang ,  Zhen Qian ,  Jing Ge ,  Zhenghong Zhang

IPC分类号： C12N9/34 ,  C12P7/06 ,  C12P19/12 ,  C12P19/02

摘要： Described are methods and compositions relating to granular starch-converting glucoamylases and α-amylases. The enzymes can be used to perform enzymatic starch hydrolysis of granular starch at or below the gelatinization temperature of insoluble granular starch.

公开(公告)号：US20180255805A1

公开(公告)日：2018-09-13

申请号：US15978899

申请日：2018-05-14

申请人： DANISCO US INC

发明人： DOUGLAS A. DALE

IPC分类号： A23K20/105 ,  C12N9/98 ,  C12N9/96 ,  C12N9/16 ,  A23K40/30 ,  A23K40/25 ,  A23K20/158 ,  A23K40/10 ,  A23K20/28 ,  A23K20/22 ,  A23K20/189 ,  A23K20/163 ,  A23K40/20

CPC分类号： A23K20/105 ,  A23K20/158 ,  A23K20/163 ,  A23K20/189 ,  A23K20/22 ,  A23K20/28 ,  A23K40/10 ,  A23K40/20 ,  A23K40/30 ,  C12N9/16 ,  C12N9/96 ,  C12N9/98

摘要： The present teachings provide an improved layered granule comprising a sandwich structure of a first and second moisture barrier layer encompassing a moisture hydrating layer. The sandwich granules can be used in a variety of contexts, including animal feed. Methods of making and using are also provided.

公开(公告)号：US20180251743A1

公开(公告)日：2018-09-06

申请号：US15984464

申请日：2018-05-21

申请人： DANISCO US INC.

发明人： MARC KOLKMAN ,  RIE MEJLDAL ,  ANJA HEMMINGSEN KELLETT SMITH ,  LILIA MARIA BABE ,  RICHARD R. BOTT

IPC分类号： C12N9/54 ,  C11D11/00 ,  C11D3/386

摘要： The present disclosure relates to serine proteases cloned from Bacillus Akibai and Bacillus Clarkii, and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.

公开(公告)号：US20180251742A1

公开(公告)日：2018-09-06

申请号：US15969954

申请日：2018-05-03

申请人： DANISCO US INC

发明人： DAVID A. ESTELL ,  AMY D. LIU ,  RONALDUS W.J. HOMMES ,  ANDREW SHAW

IPC分类号： C12N9/54 ,  C11D3/386

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38618 ,  C11D3/38636 ,  C12Y304/24027

摘要： The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.

公开(公告)号：US20180208945A1

公开(公告)日：2018-07-26

申请号：US15746479

申请日：2016-07-28

申请人： DANISCO US INC.

发明人： Kai BAO ,  Susan Mampusti MADRID ,  Brian F. SCHMIDT

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N15/80

CPC分类号： C12N15/902 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/80 ,  C12N2310/20 ,  C12N2800/80

摘要： Compositions and methods are provided for genome editing at a target site in the genome of a filamentous fungal cell. The methods and compositions disclosed are drawn to a guide polynucleotide/Cas endonuclease system and donor polynucleotides with shorter homology arms (i.e., less than 500 bps) to a genomic locus of the fungal cell.