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51.发明授权公开(公告)号：US09663559B2
公开(公告)日：2017-05-30
申请号：US15063471
申请日：2016-03-07
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
CPC classification number: B01J20/24 , B01D15/3809 , B01J20/28019 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , B01J2220/54 , C07K1/22 , C07K14/31 , C07K16/00 , C07K17/00 , C07K17/10 , C07K2317/55
Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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公开(公告)号：US20170097327A1
公开(公告)日：2017-04-06
申请号：US15379447
申请日：2016-12-14
Applicant: GE Healthcare BioProcess R&D AB
Inventor： KLAUS GEBAUER
CPC classification number: G01N30/6004 , B01D15/18 , B01D15/22 , G01N30/6017 , G01N30/6021 , G01N30/6052 , G01N30/606
Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.
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公开(公告)号：US09573973B2
公开(公告)日：2017-02-21
申请号：US14396207
申请日：2013-04-22
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Jesper Hansson , Gustav Rodrigo , Tobias E Soderman
IPC: B01D15/36 , C07K1/22 , B01D15/18 , B01J20/286 , B01J20/32 , B01D15/38 , B01J20/26 , B01J20/28 , B01J20/289 , B01J20/30 , C07H1/08 , G01N30/88
CPC classification number: B01J39/26 , B01D15/1864 , B01D15/34 , B01D15/361 , B01D15/362 , B01D15/3809 , B01J20/24 , B01J20/261 , B01J20/265 , B01J20/267 , B01J20/28014 , B01J20/28033 , B01J20/286 , B01J20/289 , B01J20/3085 , B01J20/3212 , B01J20/3278 , B01J20/3282 , B01J2220/80 , C07H1/08 , C07K1/22 , G01N2030/8827 , Y02P20/582
Abstract: The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.
Abstract translation: 本发明公开了一种从液体中的至少一种其它组分分离生物分子的方法，包括使所述液体与包含固体支持物和与所述固体支持物结合的聚合物链的分离基质接触的步骤。 聚合物链包含衍生自结构为CH 2 = CH-L-X的第一单体的单元，其中L为共价键或包含2-6个碳原子的烷基醚或羟基取代的烷基醚链，X为磺酸盐或膦酸酯基团。
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公开(公告)号：US20200299325A1
公开(公告)日：2020-09-24
申请号：US16095753
申请日：2017-05-10
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Annika Forss , Gustav José Rodrigo , Tomas Bjorkman , Mats Ander , Jesper Ulf Hansson
IPC: C07K1/22 , C07K16/00 , C07K14/31 , B01J20/32 , B01J20/26 , B01J20/285 , B01J20/286 , C07K16/12 , C07K17/10
Abstract: The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix; b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time. The alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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公开(公告)号：US10730908B2
公开(公告)日：2020-08-04
申请号：US15798784
申请日：2017-10-31
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Annika Forss , Mats Ander , Tomas Bjorkman , Hans Blom , Jesper Hansson , Gustav Rodrigo
IPC: C07K1/16 , B01D15/08 , C07K1/22 , C07K16/00 , B01J20/286 , B01J20/285 , B01D15/18 , B01D15/34 , C07K14/31
Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.
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Patent Agency Ranking
公开(公告)号：US20190218265A1
公开(公告)日：2019-07-18
申请号：US16328058
申请日：2017-09-05
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Sophia Hober , Sara Kanje , Johan Nilvebrant
CPC classification number: C07K14/4728 , C07K1/22 , C07K14/31 , C07K2319/20 , C07K2319/30 , C07K2319/705
Abstract: The present invention is within the field of protein engineering and purification. The invention relates to a target-binding polypeptide mutant of an IgG binding polypeptide, such as Protein A, Protein G, Protein L or Protein M, comprising a metal binding motif. More closely the invention relates to an Fc binding ligand comprising an engineered protein based on the Protein A derived Z domain, to which a calcium binding EF-loop has been introduced.
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公开(公告)号：US20190119318A1
公开(公告)日：2019-04-25
申请号：US16096952
申请日：2017-05-10
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav José Rodrigo , Tomas Bjorkman , Mats Ander , Jesper Ulf Hansson
Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
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公开(公告)号：US10266563B2
公开(公告)日：2019-04-23
申请号：US15308327
申请日：2015-06-04
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Jean-Luc Maloisel , Ola Lind , Bjorn Noren , Ronnie Palmgren
Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.
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公开(公告)号：US10208091B2
公开(公告)日：2019-02-19
申请号：US15526849
申请日：2015-12-11
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Tomas Bjorkman
IPC: C07K14/195 , C07K1/22 , C07K16/00
Abstract: A kappa light chain-binding polypeptide comprising or consisting essentially of one or more binding domains of Peptostreptococcus Protein L, each of said domains being selected from the group consisting of domain 2, domain 3 and domain 4.
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公开(公告)号：US20180327512A1
公开(公告)日：2018-11-15
申请号：US15525688
申请日：2015-11-11
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
IPC: C07K16/46 , B01J20/289 , B01J20/32 , B01D15/38
CPC classification number: C07K16/46 , B01D15/3809 , B01J20/289 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/31 , C07K16/00 , C07K16/065
Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.
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