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51.发明授权公开(公告)号：US08569036B2
公开(公告)日：2013-10-29
申请号：US11485575
申请日：2006-07-12
申请人： Robert Dicosimo , Sharon L. Haynie , Lixuan Lisa Huang , Fateme Sima Sariaslani , Tina K. Van Dyk , Robert J. Trotman
发明人： Robert Dicosimo , Sharon L. Haynie , Lixuan Lisa Huang , Fateme Sima Sariaslani , Tina K. Van Dyk , Robert J. Trotman
摘要： TAL cell biocatalyst was immobilized in alginate cross-linked beads using low concentrations of glutaraldehyde. The biocatalyst beads have highly stable TAL activity and mechanical strength such that they withstand prolonged recycling in production of pHCA.
摘要翻译： 使用低浓度戊二醛将TAL细胞生物催化剂固定在藻酸盐交联珠粒中。 生物催化剂珠具有高度稳定的TAL活性和机械强度，使得它们在pHCA的生产中经受长时间的再循环。
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公开(公告)号：US08486380B2
公开(公告)日：2013-07-16
申请号：US13609504
申请日：2012-09-11
申请人： Robert Dicosimo , Arie Ben-Bassat , William R. Cahill , David George Dipietro , Eugenia Costa Hann , Mark S. Payne , Richard Alan Reynolds , Raymond Richard Zolandz
发明人： Robert Dicosimo , Arie Ben-Bassat , William R. Cahill , David George Dipietro , Eugenia Costa Hann , Mark S. Payne , Richard Alan Reynolds , Raymond Richard Zolandz
IPC分类号： A61L9/00
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein are multi-component formulations for enzymatically producing aqueous solutions of peroxycarboxylic acids suitable for use in, e.g., disinfectant and/or bleaching applications. The multi-component peroxycarboxylic acid formulations comprise at least one carbohydrate esterase family 7 enzyme having perhydrolytic activity.
摘要翻译： 本文公开了用于酶促生产适用于例如消毒剂和/或漂白应用的过氧羧酸的水溶液的多组分制剂。 多组分过氧羧酸制剂包含至少一种具有过水解活性的碳水化合物酯酶家族7酶。
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53.发明授权Extraction solvents derived from oil for alcohol removal in extractive fermentation 有权萃取溶剂中的萃取溶剂，用于萃取发酵中的酒精去除公开(公告)号：US08476047B2
公开(公告)日：2013-07-02
申请号：US13692254
申请日：2012-12-03
CPC分类号： C12P7/16 , A23K10/38 , B01D3/002 , B01D11/0492 , B01D21/26 , B01D21/262 , C07C29/86 , C11B1/025 , C11B13/00 , C12F3/10 , C12M1/00 , C12M21/12 , C12M45/04 , C12M45/09 , C12N1/14 , C12N15/00 , C12P7/18 , C12P7/6418 , Y02E50/10 , Y02E50/16 , Y02E50/17 , Y02W30/74
摘要： In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant. The fermentation product extractant can be contacted with a fermentation broth for in situ removal of a product alcohol.
摘要翻译： 在酒精发酵过程中，将来自生物质的油水解成可从发酵液中原位除去产物醇如丁醇的萃取剂。 油中的甘油酯可以催化（例如，酶促）水解成游离脂肪酸，其形成产物醇的分配系数大于产物醇的生物质油的分配系数的发酵产物萃取剂。 由酒精发酵方法的原料得到的油可以通过使包含油的原料与一种或多种酶接触来水解，由此至少一部分油被水解成形成发酵产物萃取剂的游离脂肪酸，或者油可以是 在原料进料到发酵容器之前与原料分离，并且分离的油可以与酶接触以形成发酵产物萃取剂。 发酵产物萃取剂可与发酵液接触，用于原位除去产物醇。
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54.发明申请PRODUCTION OF ALCOHOL ESTERS IN SITU USING ALCOHOLS AND FATTY ACIDS PRODUCED BY MICROORGANISMS 审中-公开利用微生物生产的酒精和脂肪酸生产酒精饮料公开(公告)号：US20130040340A1
公开(公告)日：2013-02-14
申请号：US13366511
申请日：2012-02-06
申请人： Michael Dauner , Robert Dicosimo , Ranjan Patnaik
发明人： Michael Dauner , Robert Dicosimo , Ranjan Patnaik
CPC分类号： C12P7/6436 , C12P7/649 , C12P39/00 , Y02E50/13
摘要： Fatty acid alcohol esters that may be used as biodiesel are synthesized from microbially produced alcohol and fatty acids using an in situ process. The process utilizes a catalyst capable of esterifying the alcohol and fatty acid products of microorganisms, which are produced from renewable resources.
摘要翻译： 可用作生物柴油的脂肪酸醇酯由原位生产的微生物醇和脂肪酸合成。 该方法利用能够使可再生资源生产的微生物的醇和脂肪酸产物酯化的催化剂。
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公开(公告)号：US08367597B2
公开(公告)日：2013-02-05
申请号：US13213583
申请日：2011-08-19
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
摘要翻译： 本文公开了一种在具有羧酸酯的制剂中稳定CE-7酯酶的过水解酶活性的方法，其使用向CE-7酯酶和羧酸的混合物中添加基本上未溶解的缓冲剂 酯。 此外，提供包含通过本文所述方法生产的过酸的消毒剂和衣物护理制剂。
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公开(公告)号：US08337905B2
公开(公告)日：2012-12-25
申请号：US12572107
申请日：2009-10-01
申请人： Robert Dicosimo , Arie Ben-Bassat , William R. Cahill , David George Dipietro , Eugenia Costa Hann , Mark S. Payne , Richard Alan Reynolds , Raymond Richard Zolandz
发明人： Robert Dicosimo , Arie Ben-Bassat , William R. Cahill , David George Dipietro , Eugenia Costa Hann , Mark S. Payne , Richard Alan Reynolds , Raymond Richard Zolandz
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein are multi-component peroxycarboxylic acid generation systems for enzymatically producing aqueous formulations of peroxycarboxylic acids suitable for use in, e.g., disinfectant and/or bleaching applications. The multi-component peroxycarboxylic acid generation systems comprise at least one carbohydrate esterase family 7 enzyme having perhydrolytic activity.
摘要翻译： 本文公开了用于酶促生产适用于例如消毒剂和/或漂白应用的过氧羧酸的水性制剂的多组分过氧羧酸生成系统。 多组分过氧羧酸生成系统包含至少一种具有过水解活性的碳水化合物酯酶家族7酶。
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57.发明申请PRODUCTION OF ALCOHOL ESTERS AND IN SITU PRODUCT REMOVAL DURING ALCOHOL FERMENTATION 有权在酒精发酵期间生产酒精产品和脱除产品公开(公告)号：US20120208246A1
公开(公告)日：2012-08-16
申请号：US13193147
申请日：2011-07-28
申请人： Douglas Robert Anton , Stephane Francois Bazzana , Keith H. Burlew , Jelena Cirakovic , Robert Dicosimo , Bruce A. Diner , Michael Charles Grady , Brian Michael Roesch , Francis J. Woerner
发明人： Douglas Robert Anton , Stephane Francois Bazzana , Keith H. Burlew , Jelena Cirakovic , Robert Dicosimo , Bruce A. Diner , Michael Charles Grady , Brian Michael Roesch , Francis J. Woerner
CPC分类号： A23K1/007 , A23K10/12 , A23K10/38 , C12P7/16 , C12P7/62 , C12P7/649 , C12P2203/00 , Y02E50/10 , Y02E50/13 , Y02P60/873
摘要： An alcohol fermentation process and composition that includes production of alcohol esters by esterification of product alcohol in a fermentation medium with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product alcohol, such as butanol, with the carboxylic acid to form the alcohol esters. The alcohol esters can be extracted from the fermentation medium, and the product alcohol recovered from the alcohol esters. The carboxylic acid can also serve as an extractant for removal of the alcohol esters from the fermentation medium.
摘要翻译： 醇发酵方法和组合物，其包括通过在发酵培养基中与产物醇与羧酸（例如，脂肪酸）和能够酯化产物醇（例如丁醇）的催化剂（例如脂肪酶）酯化产生醇酯， 与羧酸形成醇酯。 醇酯可以从发酵培养基中提取，并从醇酯中回收产物醇。 羧酸也可以用作从发酵培养基中除去醇酯的提取剂。
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公开(公告)号：US20110305683A1
公开(公告)日：2011-12-15
申请号：US13213642
申请日：2011-08-19
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
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公开(公告)号：US07919288B2
公开(公告)日：2011-04-05
申请号：US12429563
申请日：2009-04-24
CPC分类号： C12N9/78 , C12N9/96 , C12P7/42 , C12Y305/05001
摘要： A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.
摘要翻译： 提供了一种在水性反应条件下将乙醇腈转化成乙醇酸时，提高具有腈水解酶活性的酶催化剂的比活性的方法。 包含有效量的至少一种胺保护剂提高了酶催化剂的比活性和催化生产率。
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公开(公告)号：US20100196975A1
公开(公告)日：2010-08-05
申请号：US12429554
申请日：2009-04-24
IPC分类号： C12P7/56
CPC分类号： C12N9/78 , C12N9/96 , C12P7/42 , C12Y305/05001
摘要： A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.
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