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    APPARATUS AND METHODS FOR ANALYTE MEASUREMENT AND IMMUNOASSAY
    51.
    发明申请
    APPARATUS AND METHODS FOR ANALYTE MEASUREMENT AND IMMUNOASSAY 有权
    用于分析测量和免疫测定的装置和方法

    公开(公告)号：US20090065368A1

    公开(公告)日：2009-03-12

    申请号：US12165925

    申请日：2008-07-01

    申请人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

    发明人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

    IPC分类号： G01N27/327 ,  G01N27/40

    CPC分类号： G01N33/543 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0683 ,  G01N33/54366 ,  G01N35/08 ,  G01N2035/0436

    摘要： The present invention relates to an apparatus for conducting a variety of assays for the determination of analytes in liquid samples, and relates to the methods for such assays. In particular, the invention relates to a single-use cartridge designed to be adaptable to a variety of real-time assay protocols, preferably assays for the determination of analytes in biological samples using immunosensors or other ligand/ligand receptor-based biosensor embodiments. The cartridge provides novel features for processing a metered portion of a sample, for precise and flexible control of the movement of a sample or second fluid within the cartridge, for the amending of solutions with additional compounds during an assay, and for the construction of immunosensors capable of adaptation to diverse analyte measurements. The disclosed device and methods of use enjoy substantial benefits over the prior art, including simplicity of use by an operator, rapid in situ determinations of one or more analytes, and single-use methodology that minimizes the risk of contamination of both operator and patient. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.

    摘要翻译： 本发明涉及一种用于进行各种测定液体样品中分析物测定的装置，并涉及这种测定方法。 特别地，本发明涉及一种设计成能够适用于各种实时测定方案的单次使用的盒，优选使用免疫传感器或其他基于配体/配体受体的生物传感器实施方案来测定生物样品中分析物的测定。 该盒提供用于处理样品的计量部分的新颖特征，用于精确和灵活地控制盒内样品或第二流体的运动，用于在测定过程中用附加化合物修饰溶液以及用于构建免疫传感器 能够适应不同的分析物测量。 所公开的装置和使用方法相对于现有技术具有实质性的益处，包括操作者的简单使用，一种或多种分析物的快速原位测定，以及使操作者和患者的污染风险最小化的一次性方法。 所公开的发明适用于临床诊断领域，包括在事故现场，急诊室，手术室，疗养院，重症监护室和非医疗环境中的使用。

    Apparatus and methods for analyte measurement and immunoassay
    52.
    发明授权
    Apparatus and methods for analyte measurement and immunoassay 有权
    用于分析物测量和免疫测定的装置和方法

    公开(公告)号：US07419821B2

    公开(公告)日：2008-09-02

    申请号：US10087730

    申请日：2002-03-05

    申请人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

    发明人： Graham Davis ,  Imants R. Lauks ,  Chao Lin ,  Cary James Miller

    IPC分类号： C12M3/00

    CPC分类号： G01N33/543 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0683 ,  G01N33/54366 ,  G01N35/08 ,  G01N2035/0436

    摘要： The present invention relates to an apparatus for conducting a variety of assays for the determination of analytes in liquid samples, and relates to the methods for such assays. In particular, the invention relates to a single-use cartridge designed to be adaptable to a variety of real-time assay protocols, preferably assays for the determination of analytes in biological samples using immunosensors or other ligand/ligand receptor-based biosensor embodiments. The cartridge provides novel features for processing a metered portion of a sample, for precise and flexible control of the movement of a sample or second fluid within the cartridge, for the amending of solutions with additional compounds during an assay, and for the construction of immunosensors capable of adaptation to diverse analyte measurements. The disclosed device and methods of use enjoy substantial benefits over the prior art, including simplicity of use by an operator, rapid in situ determinations of one or more analytes, and single-use methodology that minimizes the risk of contamination of both operator and patient. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.

    摘要翻译： 本发明涉及一种用于进行各种测定液体样品中分析物测定的装置，并涉及这种测定方法。 特别地，本发明涉及一种设计成适用于各种实时测定方案的一次性盒，优选使用免疫传感器或其它基于配体/配体受体的生物传感器实施方案来测定生物样品中分析物的测定。 该盒提供用于处理样品的计量部分的新颖特征，用于精确和灵活地控制盒内样品或第二流体的运动，用于在测定过程中用附加化合物修饰溶液以及用于构建免疫传感器 能够适应不同的分析物测量。 所公开的装置和使用方法相对于现有技术具有实质性的益处，包括操作者的简单使用，一种或多种分析物的快速原位测定，以及使操作者和患者的污染风险最小化的一次性方法。 所公开的发明适用于临床诊断领域，包括在事故现场，急诊室，手术，疗养院，重症监护病房和非医疗环境中的使用。

    Methods for microdispensing patterened layers
    53.
    发明授权
    Methods for microdispensing patterened layers 失效
    微分配图案的方法

    公开(公告)号：US06306594B1

    公开(公告)日：2001-10-23

    申请号：US09193370

    申请日：1998-11-17

    申请人： Stephen N. Cozzette ,  Graham Davis ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Paul Van der Werf ,  Henry J. Wieck

    发明人： Stephen N. Cozzette ,  Graham Davis ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Paul Van der Werf ,  Henry J. Wieck

    IPC分类号： G01N3350

    CPC分类号： B01L3/5027 ,  B01L3/0268 ,  B01L3/502707 ,  B01L3/5088 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0819 ,  B01L2300/089 ,  C12Q1/001 ,  G01N33/5438 ,  G01N35/0099 ,  G01N35/109 ,  G01N2035/00059 ,  G01N2035/00237 ,  G01N2035/00455 ,  G01N2035/00534 ,  G01N2035/1034 ,  G01N2035/1076 ,  Y10S435/81 ,  Y10S436/809

    摘要： An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

    摘要翻译： 描述了适用于分析生物学重要的分析物种的电子装置的微细加工的有效方法。 本发明的技术允许对在合适的衬底上建立的各种组件和层的尺寸特征进行密切的控制。 这种控制延伸到结合生物组分的装置的那些部分，使得这些装置能够用作生物传感器。 因此，本文公开的材料和方法提供了用于批量生产均匀的全微生物传感器的有效手段。 本文描述了装置本身的各种实施例，其特别适用于临床环境中的生物样品的实时分析。 特别地，本发明描述了可以使用某些基于配体/配体受体的生物传感器实施方案进行的测定。 本发明还公开了一种利用基板/标记信号产生对电化学检测特定分析物种的生物学和生理意义的新方法，其产生选自二氧和过氧化氢的电活性物质浓度的变化。

    Strip electrode with screen printing
    54.
    发明授权
    Strip electrode with screen printing 失效
    带电极丝网印刷

    公开(公告)号：US5820551A

    公开(公告)日：1998-10-13

    申请号：US470352

    申请日：1995-06-06

    申请人： Hugh Allen Oliver Hill ,  Irving John Higgins ,  James Michael McCann ,  Graham Davis

    发明人： Hugh Allen Oliver Hill ,  Irving John Higgins ,  James Michael McCann ,  Graham Davis

    IPC分类号： A61B5/00 ,  C12Q1/00 ,  C12Q1/68 ,  G01N27/403 ,  G01N33/487 ,  G01N33/543 ,  A61B5/05

    CPC分类号： A61B5/1486 ,  A61B5/14865 ,  A61B5/6848 ,  C12Q1/001 ,  C12Q1/004 ,  G01N27/4035 ,  G01N33/5438 ,  A61B2560/0418

    摘要： A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a single layer deposit of an enzyme capable of catalyzing a reaction involving the compound, a conductive material and an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing an admixture of an enzyme, a conductive material, and a mediator as a single layer onto the substrate.

    摘要翻译： 检测液体混合物中的化合物的当前代表的传感器系统具有适于可释放地附接到信号读出电路的双电极条。 条带包括适于可释放地附接到读出电路的细长支撑件（优选是平坦的）; 第一导体和第二导体各自沿着支撑件延伸并且包括用于连接到电路的装置。 定位成接触液体混合物和第一导体的活性电极包括能够催化涉及化合物，导电材料和电子介体的反应的酶的单层沉积物，其能够在酶催化反应之间传递电子 和第一个导体。 定位参考电极以接触混合物和第二导体。 该系统包括适于提供代表电流的电信号的电路。 双电极条例如通过将酶，导电材料和介体的混合物作为单层丝网印刷到基底上来制造。