Patent search ap:("LIFE TECHNOLOGIES CORPORATION") AND inv:"Kyle Gee" Page 6

51.

发明申请
ALKYNYL-DERIVATIZED CAP ANALOGS, PREPARATION AND USES THEREOF 有权
Title translation: 亚苄基衍生化的脂类似物，其制备及其用途

公开(公告)号：US20130102655A1

公开(公告)日：2013-04-25

申请号：US13655103

申请日：2012-10-18

Applicant: Life Technologies Corporation

Inventor： Anilkumar R. Kore , Shanmugasundaram Muthian , Kyle Gee

IPC: C07H19/207 , C12Q1/68 , C07D213/82 , C07H21/02 , C07C247/04

CPC classification number: C12N15/67 , A61K31/7084 , A61K31/7088 , A61K31/7115 , A61K31/712 , A61K39/12 , A61K39/39 , A61K48/00 , A61K2039/53 , A61K2039/55511 , C07C247/04 , C07C323/60 , C07D213/82 , C07H1/00 , C07H1/02 , C07H1/04 , C07H19/207 , C07H21/02 , C12N7/00 , C12N15/11 , C12N2770/36134 , C12N2770/36171 , C12P19/34 , C12Q1/68

Abstract: Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.

Abstract translation: 提供炔基衍生的帽类似物，炔基修饰的封端RNA，1,4-二取代的三唑衍生的封端RNA，制备方法，分离方法及其用途。 “点击”修改有助于检测和分离封闭的RNA，通过“点击”反应形成的1,4-二取代的三唑衍生物可用于产生RNA转录物和编码的蛋白质。

52.

发明授权
Fluorogenic pH sensitive dyes and their method of use 有权

公开(公告)号：US11867698B2

公开(公告)日：2024-01-09

申请号：US17027391

申请日：2020-09-21

Applicant: Life Technologies Corporation

Inventor： Jeffrey Dzubay , Kyle Gee , Vladimir Martin , Aleksey Rukavishnikov , Daniel Beacham

IPC: G01N33/58 , G01N33/50 , G01N33/84 , C09B11/24 , C09B11/28 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07D209/14 , C09K11/06 , C07F5/02 , C07K1/13 , C09B7/00 , C09B11/12 , C09B57/00 , C09B69/00 , G01N31/22 , G01N33/80

CPC classification number: G01N33/582 , C07D209/14 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07F5/02 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/24 , C09B11/28 , C09B57/00 , C09B69/00 , C09K11/06 , G01N31/22 , G01N33/5005 , G01N33/5058 , G01N33/5091 , G01N33/80 , G01N33/84 , G01N2333/245 , Y10T436/143333

Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I:

wherein the variables are described throughout the application.

53.

发明授权
Methods and compositions for detecting or measuring caspases or apoptosis 有权

公开(公告)号：US10941435B2

公开(公告)日：2021-03-09

申请号：US16306609

申请日：2017-06-21

Applicant: Life Technologies Corporation

Inventor： Kyle Gee , Wenjun Zhou , Shih-Jung Huang , Monica Tomaszewski

IPC: C12Q1/37 , C07K5/103 , C07K5/113 , G01N21/64 , G01N33/58 , C12Q1/48

Abstract: Provided herein are compounds, enzyme substrates, compositions, kits, uses, and methods for detecting the presence or absence of a caspase enzyme, measuring the activity of a caspase enzyme, or detecting the presence or absence of apoptosis. The detection or measurement can occur through intracellular cleavage of a compound or enzyme substrate, which can lead to an increase in fluorescence, e.g., in the violet or red channel, through liberation of a nucleic acid binding dye from a peptide, such as liberation of a DNA-binding dye from a negatively charged peptide comprising a sequence recognized and cleaved by a caspase.

54.

发明授权
Hydrazinyl and aminooxy compounds and their methods of use 有权

公开(公告)号：US10793518B2

公开(公告)日：2020-10-06

申请号：US15894057

申请日：2018-02-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun Zhou , Kyle Gee , Yolanda Tennico , Peter Slade , Hee Chol Kang , Shaheer Khan , Brian Evans , James Stray

IPC: C07C309/47 , C07D311/84 , C07C309/51 , G01N33/58

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

55.

发明授权
Compounds and methods for conjugation of biomolecules 有权

公开(公告)号：US10648985B2

公开(公告)日：2020-05-12

申请号：US16107762

申请日：2018-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee , Upinder Singh , Scott Grecian , Scott Clarke

IPC: C07D213/46 , G01N33/58 , G01N33/533 , C07D213/74 , C07D213/79 , C07D213/81 , C07D215/12 , C07D401/04 , C07D235/14 , C07D213/53 , C07D213/68 , C07D213/78 , C09B23/06 , C09B23/08 , C09B11/24 , C07D401/14 , C07K14/575

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

56.

发明授权
Protein melt analysis using dipyrrometheneboron difluoride compounds 有权

公开(公告)号：US10401366B2

公开(公告)日：2019-09-03

申请号：US15392295

申请日：2016-12-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Levente Egry , Kyle Gee

IPC: G01N33/68 , C07F5/02 , G01N33/58 , C09B57/00 , G01N33/52 , C09B23/04

Abstract: According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.

57.

发明授权
Fluorogenic pH-sensitive dyes and their methods of use (II) 有权

公开(公告)号：US10253185B2

公开(公告)日：2019-04-09

申请号：US15423890

申请日：2017-02-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee , Upinder Singh , Aleksey Rukavishnikov , Daniel Beacham , Shih-Jung Huang , Michael Janes , Wenjun Zhou

IPC: C09B11/24 , C07D311/90 , A61K49/00 , G01N33/58 , G01N33/50 , G01N33/84

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

58.

发明申请
Carbopyronone Compounds Useful as Diagnostic Adjuvants 审中-公开

公开(公告)号：US20180340928A1

公开(公告)日：2018-11-29

申请号：US16002991

申请日：2018-06-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee , Hee Chol Kang , Wenjun Zhou , Bradley Dubbels , Michael Olszowy , Michael O'Grady

IPC: G01N33/50 , C09B11/28 , C07K16/28 , C07D471/04 , C07C309/61

CPC classification number: G01N33/5005 , C07C309/61 , C07C2603/24 , C07D471/04 , C07K16/2812 , C09B11/28 , G01N33/5014 , G01N33/5044

Abstract: Described herein are fluorescent compounds and methods and comprising these compounds. The compounds disclosed herein are carbopyronine reagents that fluoresce in the red portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells.