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63 条记录 (耗时 0.049 秒)

    Method and device for improved reaction kinetics in nucleic acid hybridizations
    51.
    发明授权
    Method and device for improved reaction kinetics in nucleic acid hybridizations 失效
    用于改善核酸杂交反应动力学的方法和装置

    公开(公告)号：US5310650A

    公开(公告)日：1994-05-10

    申请号：US925322

    申请日：1992-08-04

    申请人： Michael E. McMahon ,  Julian Gordon

    发明人： Michael E. McMahon ,  Julian Gordon

    IPC分类号： B01L3/00 ,  C12Q1/68 ,  C12Q1/70 ,  G01N30/38 ,  G01N30/60 ,  G01N30/92 ,  G01N33/558 ,  G01N37/00

    CPC分类号： G01N33/558 ,  B01L3/5023 ,  C12Q1/6834 ,  C12Q1/6876 ,  C12Q1/689 ,  C12Q1/701 ,  B01L2300/0825 ,  B01L2300/087 ,  B01L2400/0406 ,  G01N30/38 ,  G01N30/6065 ,  G01N30/92 ,  Y10S435/81

    摘要： The present invention relates to methods and test strips for performing nucleic acid hybridizations on a porous chromatographic material having the capacity for rapid chromatographic solvent transport of non-immobilized reagents and reactive solution components by means of a selected chromatographic solvent. The strip includes a first end or contact site, at which chromatographic solvent transport begins, a second end, at which chromatographic solvent transport ends, and at least one zone positioned between the first and second ends. The zone is impregnated with a capture nucleotide sequence which is immobilized against solvent transport and which is capable of hybridization with a complementary nucleotide sequence or probe so as to render it immobilized in the capture zone. The strip may further include a probe zone between the first end and the capture zone, wherein a labeled probe is impregnated such that it is mobilized by the solvent and transported to the capture zone where hybridization can occur. Interfering sample components and unhybridized nucleic acids are cleared from the capture zone by chromatographic solvent transport to form an inherent wash.

    摘要翻译： 本发明涉及用于在多孔层析材料上进行核酸杂交的方法和测试条，该多孔层析材料具有通过选择的色谱溶剂快速色谱溶剂转运非固定化试剂和反应溶液组分的能力。 条带包括开始色谱溶剂转移的第一端或接触位置，色谱溶剂输送结束的第二端和位于第一端和第二端之间的至少一个区。 该区域被捕获核苷酸序列浸渍，该捕获核苷酸序列固定化以抵抗溶剂转运并且能够与互补核苷酸序列或探针杂交，以使其固定在捕获区中。 条带还可以包括在第一端和捕获区之间的探针区，其中标记的探针被浸渍，使得其被溶剂移动并被运送到可以发生杂交的捕获区。 干扰样品组分和未杂交的核酸通过色谱溶剂转移从捕获区域中清除，形成固有的洗涤液。

    Electrokinetic device for capturing assayable agents in a dielectric fluid
    53.
    发明授权
    Electrokinetic device for capturing assayable agents in a dielectric fluid 有权

    公开(公告)号：US09618431B2

    公开(公告)日：2017-04-11

    申请号：US12955150

    申请日：2010-11-30

    申请人： Julian Gordon ,  Prasanthi Gandhi

    发明人： Julian Gordon ,  Prasanthi Gandhi

    IPC分类号： G01N1/40

    CPC分类号： G01N1/40 ,  G01N1/2202 ,  G01N1/2273 ,  G01N2001/245 ,  G01N2001/4038 ,  G01N2015/0046 ,  Y10T436/255

    摘要： Electrokinetic devices and methods are described with the purpose of collecting assayable agents from a dielectric fluid medium. Electrokinetic flow may be induced by the use of plasma generation at high voltage electrodes and consequent transport of charged particles in an electric voltage gradient. In one embodiment, the agents are directed electrokinetically to the sample collection assay device with no intermediate transfer steps. The agents are directed by creation of an electrokinetic potential well, which will effect their capture on to an assay device. Environmental agents such as biowarfare agents, pathogens, allergens or pollutants are collected autonomously on to the assay device, without any human intervention. The dielectric fluid medium, such as air, is sampled by electrokinetic propulsion with no moving parts or optionally, by transporting the dielectric fluid by a fan, pump or by breath. A further embodiment for collection of pathogen samples entails breathing into a tube where the sample is exposed to an electric plasma in the neighborhood of a high voltage electrode or electrodes, further transported by the breath through a potential well created at a sample collection device, where charge particles are electroprecipitated. The dielectric fluid medium may further include non-conductive liquids, such as oils. Oils may be sampled for the presence of contaminants, contaminating organisms or bio-degrading organisms.

    ELECTROKINETIC DEVICE FOR CAPTURING ASSAYABLE AGENTS IN A DIELECTRIC FLUID
    54.
    发明申请
    ELECTROKINETIC DEVICE FOR CAPTURING ASSAYABLE AGENTS IN A DIELECTRIC FLUID 有权
    用于在电介质流体中捕获测定剂的电动装置

    公开(公告)号：US20140206034A1

    公开(公告)日：2014-07-24

    申请号：US13747820

    申请日：2013-01-23

    申请人： Julian Gordon ,  Prasanthi Gandhi

    发明人： Julian Gordon ,  Prasanthi Gandhi

    IPC分类号： C12Q1/24

    CPC分类号： C12Q1/24 ,  G01N1/2202

    摘要： Electrokinetic devices and methods are described with the purpose of collecting assayable agents from a dielectric fluid medium. Electrokinetic flow may be induced by the use of plasma generation at high voltage electrodes and consequent transport of charged particles in an electric voltage gradient. The agents are directed by creation of an electrokinetic potential well, which will affect their capture on to an assay device. The dielectric fluid medium, such as air, is sampled by electrokinetic propulsion with no moving parts or optionally, by transporting the dielectric fluid by a fan, pump or by breath.

    摘要翻译： 描述电动装置和方法的目的是从介电流体介质中收集可分析试剂。 电动流动可以通过在高电压电极中使用等离子体产生并由此带电粒子在电压梯度中的传输来诱导。 药剂通过产生电动势阱来引导，这将影响它们捕获到测定装置上。 诸如空气的电介质流体介质通过没有移动部件的电动推进进行采样，或者可选地，通过风扇，泵或呼吸传送电介质流体。

    INTEGRATED SYSTEM FOR SAMPLING AND ANALYSIS
    56.
    发明申请
    INTEGRATED SYSTEM FOR SAMPLING AND ANALYSIS 有权
    用于采样和分析的集成系统

    公开(公告)号：US20130029408A1

    公开(公告)日：2013-01-31

    申请号：US13559911

    申请日：2012-07-27

    申请人： Julian Gordon

    发明人： Julian Gordon

    IPC分类号： C12M1/34 ,  C12M1/42

    CPC分类号： B03C3/366 ,  B03C3/12 ,  B03C3/41 ,  B03C3/47 ,  B03C2201/26 ,  G01N1/2273 ,  G01N1/40 ,  G01N33/497 ,  G01N2001/2285

    摘要： There is disclosed a device for determination of a sample from a dielectric fluid medium for a bio-specific assay device, comprising: a sampling device and a biosensor, wherein the sampling device concentrates a sample from dielectric medium by electrically focusing the sample on to a capture element and wherein said biosensor is fluidically linked to said capture element thus providing sampling and determination in a unitary device.

    摘要翻译： 公开了一种用于测定来自用于生物特异性测定装置的介电流体介质的样品的装置，包括：采样装置和生物传感器，其中采样装置通过将样品电聚焦到 捕获元件，并且其中所述生物传感器与所述捕获元件流体连接，从而在单一设备中提供采样和确定。

    Electrokinetic device for capturing assayable agents in a dielectric fluid
    58.
    发明授权
    Electrokinetic device for capturing assayable agents in a dielectric fluid 有权
    用于在介电流体中捕获分析试剂的电动装置

    公开(公告)号：US08038944B1

    公开(公告)日：2011-10-18

    申请号：US13028495

    申请日：2011-02-16

    申请人： Julian Gordon ,  Prasanthi Gandhi

    发明人： Julian Gordon ,  Prasanthi Gandhi

    IPC分类号： G01N27/00

    CPC分类号： G01N1/40 ,  G01N1/2202 ,  G01N1/2273 ,  G01N2001/245 ,  G01N2001/4038 ,  G01N2015/0046 ,  Y10T436/255

    摘要： Electrokinetic devices and methods are described with the purpose of collecting assayable agents from a dielectric fluid medium. Electrokinetic flow may be induced by the use of plasma generation at high voltage electrodes and consequent transport of charged particles in an electric voltage gradient. In one embodiment, the agents are directed electrokinetically to the sample collection assay device with no intermediate transfer steps. The agents are directed by creation of an electrokinetic potential well, which will effect their capture on to an assay device. Environmental agents such as biowarfare agents, pathogens, allergens or pollutants are collected autonomously on to the assay device, without any human intervention. The dielectric fluid medium, such as air, is sampled by electrokinetic propulsion with no moving parts or optionally, by transporting the dielectric fluid by a fan, pump or by breath. A further embodiment for collection of pathogen samples entails breathing into a tube where the sample is exposed to an electric plasma in the neighborhood of a high voltage electrode or electrodes, further transported by the breath through a potential well created at a sample collection device, where charge particles are electroprecipitated. The dielectric fluid medium may further include non-conductive liquids, such as oils. Oils may be sampled for the presence of contaminants, contaminating organisms or bio-degrading organisms.

    摘要翻译： 描述电动装置和方法的目的是从介电流体介质中收集可分析试剂。 电动流动可以通过在高电压电极中使用等离子体产生并由此带电粒子在电压梯度中的传输来诱导。 在一个实施方案中，将试剂电动定向于样品采集测定装置，无中间转移步骤。 这些试剂通过产生电动势阱来引导，这将影响其捕获到测定装置上。 环境试剂如生物灭菌剂，病原体，过敏原或污染物被自动收集到测定装置上，无需人为干预。 诸如空气的电介质流体介质通过没有移动部件的电动推进进行采样，或者可选地，通过风扇，泵或呼吸传送电介质流体。 用于收集病原体样品的另一实施例需要呼吸到管中，其中样品暴露于高压电极或电极附近的电等离子体，并进一步通过呼吸运送通过样品收集装置产生的潜在井，其中 电荷颗粒被电沉积。 介电流体介质还可以包括非导电液体，例如油。 可能为污染物，污染生物体或生物降解生物体的存在而对油进行采样。