公开(公告)号：US20160341737A1

公开(公告)日：2016-11-24

申请号：US15167513

申请日：2016-05-27

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Xuanjun Zhang ,  Jiangbo Yu ,  Fangmao Ye

IPC: G01N33/58 ,  C09K11/06 ,  C09K11/02 ,  C08J3/11 ,  C08G61/12

Abstract: The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.

公开(公告)号：US20150037259A1

公开(公告)日：2015-02-05

申请号：US14373835

申请日：2013-02-01

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Yuhui Jin ,  Fangmao Ye ,  Changfeng Wu ,  Yang-Hsiang Chan

IPC: G01N33/58 ,  A61K49/00

CPC classification number: G01N33/588 ,  A61K49/0065 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y40/00 ,  H01L51/0036 ,  H01L51/0039 ,  H01L51/0043 ,  H01L51/5012

Abstract: Polymer nanoparticles and related methods include polymer dots having a coating including a polyelectrolyte polymer. The polymer dots can have a polyelectrolyte coating that can improve colloidal stability of the particles as compared to polymer dots not having the coating. A method of preparing a population of nanoparticles. The methods can include, e.g., providing the population of nanoparticles having a condensed semiconducting polymer; and combining, in a first aqueous solution comprising polyelectrolytes, the population of nanoparticles having the condensed semiconducting polymer to form a population of nanoparticles having a polyelectrolyte coating surrounding the condensed semiconducting polymer of each of the nanoparticles in the population. The methods can include a step of forming the condensed semiconducting polymer using nanoprecipitation or miniemulsion techniques. The polyelectrolyte coating can completely surround the condensed semiconducting polymer.

Abstract translation: 聚合物纳米粒子和相关方法包括具有包括聚电解质聚合物的涂层的聚合物点。 与不具有涂层的聚合物点相比，聚合物点可以具有可以改善颗粒的胶体稳定性的聚电解质涂层。 一种制备纳米颗粒群体的方法。 所述方法可以包括例如提供具有冷凝的半导体聚合物的纳米颗粒群; 并且在包含聚电解质的第一水溶液中混合具有所述缩合的半导体聚合物的纳米颗粒群以形成纳米颗粒群，所述纳米颗粒群包含围绕群体中每个纳米颗粒的缩合半导体聚合物的聚电解质涂层。 该方法可以包括使用纳米沉淀或微乳液技术形成冷凝的半导体聚合物的步骤。 聚电解质涂层可以完全包围冷凝的半导体聚合物。

公开(公告)号：US20140220673A1

公开(公告)日：2014-08-07

申请号：US14170381

申请日：2014-01-31

Applicant: University of Washington

Inventor： Daniel T. Chiu ,  Jason S. Kuo

IPC: G01N1/34

CPC classification number: G01N1/34 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12N5/0693

Abstract: Embodiments in accordance with the present invention relate to methods and apparatuses for concentrating and isolating Circulating Tumor Cells (CTCs) from body fluids. One embodiment of the present invention includes a micro-fabricated or nano-fabricated device having channels configured for separating and excluding. Embodiments in accordance with the present invention utilize features that reduce the hydrodynamic pressure experienced by the cells during the separation, isolation and concentration processes, and therefore reduce the likelihood of cell lysis or other damage to the cells.

Abstract translation: 根据本发明的实施例涉及用于从体液浓缩和分离循环肿瘤细胞（CTC）的方法和装置。 本发明的一个实施例包括具有被配置为分离和排除的通道的微制造或纳米制造装置。 根据本发明的实施方案利用降低细胞在分离，分离和浓缩过程中所经历的流体动力学压力的特征，并因此降低细胞裂解或细胞的其它损伤的可能性。

公开(公告)号：US12216124B2

公开(公告)日：2025-02-04

申请号：US18058458

申请日：2022-11-23

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Xuanjun Zhang ,  Jiangbo Yu ,  Fangmao Ye

IPC: C09K11/06 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00 ,  C08G61/12 ,  C08J3/11 ,  C09K11/02 ,  G01N33/58 ,  H10K85/10

Abstract: The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.

公开(公告)号：US20240254288A1

公开(公告)日：2024-08-01

申请号：US18317288

申请日：2023-05-15

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Yu Rong ,  Yong Zhang ,  Yi-Che Wu ,  Yang-Hsiang Chan ,  Xuanjun Zhang ,  Jiangbo Yu ,  Wei Sun

IPC: C08G75/32 ,  A61K49/00 ,  B82Y30/00 ,  B82Y40/00 ,  C08G79/00 ,  C09B69/10 ,  G01N21/64 ,  G01N33/58 ,  H10K50/11 ,  H10K85/10

CPC classification number: C08G75/32 ,  A61K49/0019 ,  A61K49/0067 ,  C08G79/00 ,  C09B69/105 ,  G01N33/582 ,  G01N33/587 ,  H10K85/10 ,  H10K85/113 ,  H10K85/115 ,  H10K85/151 ,  B82Y30/00 ,  B82Y40/00 ,  G01N2021/6439 ,  H10K50/11

Abstract: Polymers, monomers, chromophoric polymer dots and related methods are provided. Highly fluorescent chromophoric polymer dots with narrow-band emissions are provided. Methods for synthesizing the chromophoric polymers, preparation methods for forming the chromophoric polymer dots, and biological applications using the unique properties of narrow-band emissions are also provided.

公开(公告)号：US11982678B2

公开(公告)日：2024-05-14

申请号：US16171918

申请日：2018-10-26

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. Chiu ,  Perry G. Schiro ,  Jason S. Kuo

IPC: G01N33/58 ,  G01N21/25 ,  G01N22/00 ,  G01N33/53 ,  G01N33/574 ,  G01N33/68 ,  G01N15/1433 ,  G01N21/64

CPC classification number: G01N33/6893 ,  G01N21/25 ,  G01N22/00 ,  G01N33/5302 ,  G01N33/5308 ,  G01N33/57407 ,  G01N33/582 ,  G01N15/1433 ,  G01N21/64 ,  Y02A50/30

Abstract: Provided herein, among other aspects, are methods and apparatuses for ranking aliquots from a suspension containing bioparticles. In certain embodiments, the bioparticles may be cells, organelles, proteins, DNAs, debris of biological origin, microbeads coated with biological compounds, or viral particles. As such, the methods and apparatuses provided herein may be used to quantify rare cells such as circulating cancer cells, fetal cells and other rare cells present in bodily fluids for disease diagnosis, prognosis, or treatment.

公开(公告)号：US11939626B2

公开(公告)日：2024-03-26

申请号：US17813415

申请日：2022-07-19

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. Chiu ,  Jason E. Kreutz ,  Gloria S. Yen ,  Bryant S. Fujimoto

IPC: C12Q1/6816 ,  G01N15/14 ,  G01N15/1434 ,  G01N21/47 ,  G01N21/64 ,  G06T7/62 ,  G06V20/69

CPC classification number: C12Q1/6816 ,  G01N15/1434 ,  G01N21/47 ,  G01N21/6428 ,  G06T7/62 ,  G06V20/695 ,  G01N2015/1445 ,  G01N2021/6439 ,  G06T2207/10056 ,  G06T2207/30072 ,  C12Q1/6816 ,  C12Q2531/113 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2563/159

Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

公开(公告)号：US20240069032A1

公开(公告)日：2024-02-29

申请号：US18492120

申请日：2023-10-23

Applicant: University of Washington ,  Clemson University Research Foundation

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Jason McNeil ,  Jiangbo Yu

IPC: G01N33/58 ,  C09K11/06

CPC classification number: G01N33/587 ,  C09K11/06 ,  G01N33/582

Abstract: The present invention provides, among other aspects, functionalized chromophoric polymer dots comprising a hydrophobic core and a hydrophilic cap, and bioconjugates thereof. Also provided are improved methods for preparing functionalized chromophoric polymer dots. Methods for in vivo imaging and molecular labeling are also disclosed.

公开(公告)号：US11753681B2

公开(公告)日：2023-09-12

申请号：US16761235

申请日：2018-11-01

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Daniel T. Chiu ,  Jiangbo Yu ,  Jason E. Kreutz ,  Jiasi Wang

IPC: C12Q1/686

CPC classification number: C12Q1/686 ,  C12Q2527/107 ,  C12Q2531/125 ,  C12Q2537/143

Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the detection of nucleic acids and proteins. Also provided are methods, devices, systems, and compositions for improved detection and quantification of target molecules using encoded probes. In certain aspects, the methods, devices, and systems provided herein are useful in multiplexed digital assays. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of compartmentalized volumes in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

公开(公告)号：US11668713B2

公开(公告)日：2023-06-06

申请号：US16347502

申请日：2017-11-16

Applicant: University of Washington

Inventor： Daniel T. Chiu ,  Chun-Ting Kuo ,  Li Wu

IPC: G01N33/554 ,  G01N33/533 ,  B01L3/00 ,  G01N15/14 ,  G01N21/64

CPC classification number: G01N33/554 ,  B01L3/502715 ,  G01N15/1484 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N33/533 ,  B01L2300/047 ,  B01L2300/06 ,  B01L2300/0627 ,  B01L2300/0819 ,  B01L2300/0883 ,  B01L2300/18 ,  G01N2021/6432

Abstract: Methods and systems for improved labeling and/or de-labeling a molecule or cell in the context of scientific experimentation, industrial applications, and clinical investigation, including the means to repeat the process of labeling and de-labeling in an efficient manner.