公开(公告)号：US09938340B2

公开(公告)日：2018-04-10

申请号：US14877475

申请日：2015-10-07

申请人： HOFFMANN-LA ROCHE INC.

发明人： Herbert Andres ,  David Casagolda Vallribera ,  Hartmut Duefel ,  Michael Gerg ,  Christian Scholz ,  Michael Schraeml

IPC分类号： C07K16/22 ,  C12N9/90 ,  C07K16/12 ,  G01N33/74 ,  C07K16/40 ,  G01N33/573 ,  C07K14/475 ,  C07K14/65

CPC分类号： C07K16/22 ,  C07K14/475 ,  C07K14/65 ,  C07K16/1203 ,  C07K16/40 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/23 ,  C07K2319/24 ,  C07K2319/35 ,  C07K2319/41 ,  C07K2319/43 ,  C12N9/90 ,  C12Y502/01008 ,  G01N33/573 ,  G01N33/74 ,  G01N2333/99

摘要： Herein is reported a fusion polypeptide according to formula I NH2—S2—X1—S1—COOH  (formula I) wherein X1 comprises either a random amino acid sequence or an amino acid sequence derived from a first polypeptide, S2 and S1 are non-overlapping amino acid sequences derived from a second polypeptide, and — denotes a peptide bond, wherein the second polypeptide is a polypeptide with peptidyl-prolyl cis/trans-isomerase activity (PPIase activity) or is derived from the FKBP-fold domain family, wherein X1 is inserted in place of the insert-in-flap-domain of the second polypeptide.

公开(公告)号：US09879266B2

公开(公告)日：2018-01-30

申请号：US14937607

申请日：2015-11-10

申请人： Thermo Fisher Scientific Inc.

发明人： Anastasia Khvorova ,  Angela Reynolds ,  Devin Leake ,  William Marshall ,  Steven Read ,  Stephen Scaringe

IPC分类号： A61K48/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/11 ,  C12N15/113 ,  A61K31/713 ,  C12N15/10 ,  C12N15/82

CPC分类号： C12N15/1137 ,  A61K31/713 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1135 ,  C12N15/1136 ,  C12N15/1138 ,  C12N15/8218 ,  C12N2310/14 ,  C12N2310/31 ,  C12N2310/322 ,  C12N2320/10 ,  C12N2320/11 ,  C12N2320/13 ,  C12Y113/12007 ,  C12Y502/01008

摘要： Efficient sequence specific gene silencing is possible through the use of siRNA technology. Be selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods compositions, and kits generated through rational design of siRNAs are disclosed, including those directed to the nucleotide sequences for HAO1.

公开(公告)号：US20170349904A1

公开(公告)日：2017-12-07

申请号：US15679097

申请日：2017-08-16

申请人： Thermo Fisher Scientific Inc.

发明人： Anastasia Khvorova ,  Angela Reynolds ,  Devin Leake ,  William Marshall ,  Steven Read ,  Stephen Scaringe

IPC分类号： C12N15/113 ,  C12N15/82 ,  C12N15/11 ,  A61K31/713 ,  C12N15/10

CPC分类号： C12N15/1138 ,  A61K31/713 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1135 ,  C12N15/1136 ,  C12N15/1137 ,  C12N15/8218 ,  C12N2310/14 ,  C12N2320/10 ,  C12N2320/11 ,  C12N2320/13 ,  C12N2330/31 ,  C12Y113/12007 ,  C12Y502/01008

摘要： Efficient sequence specific gene silencing is possible through the use of siRNA technology. Be selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods compositions, and kits generated through rational design of siRNAs are disclosed, including those directed to the nucleotide sequences for F12.

公开(公告)号：US09821012B2

公开(公告)日：2017-11-21

申请号：US15419729

申请日：2017-01-30

申请人： The Regents of the University of California

发明人： Chia-Yung Wu ,  James Onuffer ,  Wendell A. Lim

IPC分类号： C12N5/071 ,  C12N5/16 ,  A61K35/17 ,  C07K14/705 ,  C07K16/18 ,  C07K16/28 ,  C07K14/725 ,  C12N9/90 ,  C12N9/14 ,  C12N5/0783 ,  A61K47/68 ,  A61K39/00

CPC分类号： A61K35/17 ,  A61K47/6891 ,  A61K2039/505 ,  C07K14/705 ,  C07K14/7051 ,  C07K14/70521 ,  C07K14/70575 ,  C07K14/70578 ,  C07K16/18 ,  C07K16/2803 ,  C07K16/2866 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/03 ,  C07K2319/20 ,  C07K2319/33 ,  C07K2319/74 ,  C12N5/0636 ,  C12N9/14 ,  C12N9/90 ,  C12N2510/00 ,  C12Y502/01008

摘要： The present disclosure provides a heterodimeric, conditionally active chimeric antigen receptor (CAR), and a nucleic acid comprising a nucleotide sequence encoding the CAR. The present disclosure provides cells genetically modified to produce the CAR. A CAR of the present disclosure can be used in various methods, which are also provided.

公开(公告)号：US20170198315A1

公开(公告)日：2017-07-13

申请号：US15326077

申请日：2015-08-21

申请人： Temasek Life Sciences Laboratory Limited

发明人： Yanbin LIU ,  Chong Mei KOH ,  Lianghui JI

IPC分类号： C12P7/64 ,  C12N15/52 ,  C12N9/10 ,  C12N9/88 ,  C12N9/00 ,  C12N9/04 ,  C12N9/02 ,  C12N9/90

CPC分类号： C12P7/6427 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0071 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12N15/52 ,  C12N15/81 ,  C12P7/6463 ,  C12Y101/0104 ,  C12Y102/01003 ,  C12Y102/01012 ,  C12Y114/19 ,  C12Y114/19001 ,  C12Y114/19003 ,  C12Y114/19006 ,  C12Y203/0102 ,  C12Y203/03008 ,  C12Y401/01001 ,  C12Y402/01011 ,  C12Y502/01008 ,  C12Y602/01003 ,  C12Y604/01002

摘要： The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of polyunsaturated fatty acids (PUFA) in fungal hosts selected from Rhodosporidium and Rhodotorula genera. The present invention further relates to a modified fungal host cell having reduced native aldehyde dehydrogenase (ALD 1) enzyme activity, and methods for producing omega-3 and omega-6 fatty acids and triacylglycerides, by growing said fungal host cell under suitable conditions.

公开(公告)号：US20160311934A1

公开(公告)日：2016-10-27

申请号：US14777908

申请日：2014-03-20

申请人： FRESENIUS KABI DEUTSCHLAND GMBH

发明人： Norbert ZANDER ,  Sabrina HUMMEL ,  Ulrike WEDEMEYER ,  Thomas HEY ,  Dominik HECKMANN

IPC分类号： C08B31/12 ,  A61K38/17 ,  C12N9/90 ,  A61K38/52 ,  A61K38/38 ,  C12N9/96 ,  A61K47/48 ,  A61K38/57

CPC分类号： C08B31/125 ,  A61K38/1709 ,  A61K38/1793 ,  A61K38/385 ,  A61K38/52 ,  A61K38/57 ,  A61K47/61 ,  C12N9/90 ,  C12N9/96 ,  C12Y502/01008

摘要： The present invention relates to a hydroxyalkyl starch (HAS) derivative of formula (I) wherein F1 is a functional group comprising the group —NR′—, with R′ being H or alkyl; L is a spacer bridging F1 and S; wherein HAS′ is the remainder of the HAS molecule, Rb and Rc are —[(CR1R2)mO]n—H and are the same or different from each other; Ra is —[(CR1R2)mO]n—H with HAS′ being the remainder of the hydroxyalkyl starch molecule, or Ra is HAS″ with HAS′ and HAS″ together being the remainder of the hydroxyalkyl starch molecule; R1 and R2 are independently hydrogen or an alkyl group having from 1 to 4 carbon atoms, m is 2 to 4, wherein R1 and R2 are the same or different from each other in the m groups CR1R2; n is from 0 to 6.

公开(公告)号：US09464322B2

公开(公告)日：2016-10-11

申请号：US13608596

申请日：2012-09-10

申请人： Philip W. Landfield ,  John Christopher Gant ,  Eric M. Blalock ,  Kuey-Chu Chen ,  Olivier Thibault ,  Nada Porter

发明人： Philip W. Landfield ,  John Christopher Gant ,  Eric M. Blalock ,  Kuey-Chu Chen ,  Olivier Thibault ,  Nada Porter

IPC分类号： A61K48/00 ,  C12Q1/68 ,  A61K38/17 ,  A61K38/52

CPC分类号： C12Q1/6883 ,  A61K38/1709 ,  A61K38/1738 ,  A61K38/52 ,  C12Q2600/136 ,  C12Q2600/158 ,  C12Y502/01008

摘要： The subject technology relates, in part, to a method of treating Alzheimer's Disease (AD), early-stage AD, elevated risk of AD, mild cognitive impairment (MCI), or other forms of age-related cognitive decline in a subject in need thereof by administering to the subject a molecule that promotes calcium-release stabilization in ryanodine receptors (RyRs) and/or inosital triphosphate receptors (InsP3Rs) in brain cells. Diagnostic methods using calcium-release stabilizing immunophilins, junctophilins or calmodulin are also disclosed.

摘要翻译： 主题技术部分涉及一种治疗阿尔茨海默病（AD），早期AD，升高的AD风险，轻度认知障碍（MCI）或其他形式的年龄相关的认知衰退的方法 其通过向受试者施用促进脑细胞中兰诺定受体（RyR）和/或三磷酸肌酸受体（InsP3Rs）中钙释放稳定化的分子。 还公开了使用钙释放稳定的免疫亲和素，亲油素或钙调蛋白的诊断方法。

公开(公告)号：US20160201065A1

公开(公告)日：2016-07-14

申请号：US14937607

申请日：2015-11-10

申请人： Thermo Fisher Scientific Inc.

发明人： Anastasia Khvorova ,  Angela Reynolds ,  Devin Leake ,  William Marshall ,  Steven Read ,  Stephen Scaringe

IPC分类号： C12N15/113

CPC分类号： C12N15/1137 ,  A61K31/713 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1135 ,  C12N15/1136 ,  C12N15/1138 ,  C12N15/8218 ,  C12N2310/14 ,  C12N2310/31 ,  C12N2310/322 ,  C12N2320/10 ,  C12N2320/11 ,  C12N2320/13 ,  C12Y113/12007 ,  C12Y502/01008

摘要： Efficient sequence specific gene silencing is possible through the use of siRNA technology. Be selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods compositions, and kits generated through rational design of siRNAs are disclosed, including those directed to the nucleotide sequences for HAO1.

摘要翻译： 通过使用siRNA技术，有效的序列特异性基因沉默是可能的。 通过合理设计选择特定的siRNA，可以最大限度地发挥有效的基因沉默试剂的产生，以及沉默基因的方法。 公开了通过合理设计siRNA产生的方法组合物和试剂盒，包括针对HAO1的核苷酸序列的组合物和试剂盒。

公开(公告)号：US20160194646A1

公开(公告)日：2016-07-07

申请号：US14936403

申请日：2015-11-09

申请人： Thermo Fisher Scientific Inc.

发明人： Anastasia Khvorova ,  Angela Reynolds ,  Devin Leake ,  William Marshall ,  Steven Read ,  Stephen Scaringe

IPC分类号： C12N15/113

CPC分类号： C12N15/1138 ,  A61K31/713 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1135 ,  C12N15/1136 ,  C12N15/1137 ,  C12N15/8218 ,  C12N2310/14 ,  C12N2320/10 ,  C12N2320/11 ,  C12N2320/13 ,  C12N2330/31 ,  C12Y113/12007 ,  C12Y502/01008

摘要： Efficient sequence specific gene silencing is possible through the use of siRNA technology. Be selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods compositions, and kits generated through rational design of siRNAs are disclosed, including those directed to the nucleotide sequences for F12.

摘要翻译： 通过使用siRNA技术，有效的序列特异性基因沉默是可能的。 通过合理设计选择特定的siRNA，可以最大限度地发挥有效的基因沉默试剂的产生，以及沉默基因的方法。 公开了通过合理设计siRNA产生的方法组合物和试剂盒，包括涉及F12核苷酸序列的组合物和试剂盒。

公开(公告)号：US20160177328A1

公开(公告)日：2016-06-23

申请号：US14785665

申请日：2014-04-17

申请人： BASE PLANT SCIENCE COMPANY GMBH

发明人： Yves Hatzfeld ,  Jonathan R. HOWARTH ,  Malcolm J. HAWKESFORD

IPC分类号： C12N15/82 ,  C12N9/90 ,  C12N9/02

CPC分类号： C12N15/8261 ,  C07K14/415 ,  C12N9/0036 ,  C12N9/90 ,  C12N15/8271 ,  C12N15/8273 ,  C12Y106/05005 ,  C12Y106/99 ,  C12Y502/01008 ,  Y02A40/146

摘要： A method for enhancing various economically important yield-related traits in plants. A method for enhancing one or more yield-related traits in plants by modulating expression in plants of a nucleic acid encoding a FKBP 16-3 (FK506-binding protein) or a quinone reductase-related (QRR) polypeptide. Plants having modulated expression of a nucleic acid encoding a FKBP 16-3 or a QRR polypeptide, which plants have one or more enhanced yield-related traits compared to control plants. FKBP 16-3 encoding and QRR nucleic acids, and constructs comprising the same, useful in performing the methods.

摘要翻译： 一种增强植物中各种经济上重要的产量相关性状的方法。 通过调节编码FKBP 16-3（FK506结合蛋白）或醌还原酶相关（QRR）多肽的核酸的植物中的表达来增强植物中一种或多种产量相关性状的方法。 已经调节了编码FKBP 16-3或QRR多肽的核酸表达的植物，与对照植物相比，植物具有一个或多个增强的产量相关性状。 FKBP 16-3编码和QRR核酸，以及包含其的构建体，可用于实施方法。