利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

97 条记录 (耗时 0.03 秒)

    Enone reductase
    61.
    发明申请
    Enone reductase 有权
    Enone还原酶

    公开(公告)号：US20050106696A1

    公开(公告)日：2005-05-19

    申请号：US10505396

    申请日：2003-02-14

    申请人： Michihiko Kataoka ,  Sakayu Shimizu

    发明人： Michihiko Kataoka ,  Sakayu Shimizu

    IPC分类号： C12N9/02 ,  C12P7/26 ,  C12R1/72 ,  C07H21/04 ,  C12N1/18 ,  C12N15/74

    CPC分类号： C12N9/001 ,  C12P7/26

    摘要： An isolated enone reductase characterized by a molecular mass of 61300+/−5000 Da; NADPH and NADPH as co-factor; a temperature optimum of 55-60° C. at pH 7.4; a pH optimum of 4.5-8.5 and substrate specificity on ∝,β-unsatured ketones; a process for producing it from a microorganism and a process for producing levodione from ketoisophorone using such reductase.

    摘要翻译： 分离的烯酮还原酶，其特征在于分子量为61300 +/- 5000Da; NADPH和NADPH作为辅因子; 温度最适为55-60℃，pH 7.4; pH最适为4.5-8.5，底物特异性为α，β-不熟酮; 从微生物生产它的方法和使用这种还原酶从酮异二酮生产左旋二酮的方法。

    Method for producing trifluoro-3(r)-hydroxybutyric acid derivatives
    62.
    发明授权
    Method for producing trifluoro-3(r)-hydroxybutyric acid derivatives 失效
    制备三氟-3（r） - 羟基丁酸衍生物的方法

    公开(公告)号：US06893861B1

    公开(公告)日：2005-05-17

    申请号：US09622385

    申请日：1999-02-18

    申请人： Michael Petersen ,  Olwen Birch ,  Sakayu Shimizu ,  Andreas Kjener ,  Marie-Luise Hischier ,  Susanne Thöni

    发明人： Michael Petersen ,  Olwen Birch ,  Sakayu Shimizu ,  Andreas Kjener ,  Marie-Luise Hischier ,  Susanne Thöni

    IPC分类号： C12N15/09 ,  C12N9/02 ,  C12N9/04 ,  C12N15/53 ,  C12P7/42 ,  C12P7/62 ,  C12P11/00 ,  C12P13/02 ,  C12R1/19 ,  C12P7/40

    CPC分类号： C12N9/0008 ,  C12N9/0006 ,  C12P7/42 ,  C12P7/62 ,  C12P11/00 ,  C12P13/02

    摘要： The invention relates to a biotechnological method for producing trifluoro-3(R)-hydroxybutyric acid derivatives of the general formula (I), where R1 represents —OR2, where R2 is hydrogen, C1-10 alkyl, C1-10 alkenyl, C3-8 cy-cloalkyl, aryl, alkoxyalkyl or alkoxyalkoxyalkyl; —NR3R4, where R3 and R4 are the same or different and represent hydrogen, C1-10 alkyl, C1-10 alkenyl, C3-8 cycloalkyl or aryl; or —SR5, where R5 represents hydrogen, C1-10 alkyl, C1-10 alkenyl, aryl or C3-8 cycloalkyl, based on a trifluoroacetoacetic acid derivative of the general formula (II), where R1 has the meaning given above, by means of micro-organisms which are able to reduce a carbonyl function or by means of a cell-free enzyme extract of said micro-organisms.

    摘要翻译： 本发明涉及一种生产通式（I）的三氟-3（R） - 羟基丁酸衍生物的生物技术方法，其中R 1表示-OR 2，其中 R 2是氢，C 1-10烷基，C 1-10 1-10烯基，C 3-8环 芳基，烷氧基烷基或烷氧基烷氧基烷基; 其中R 3和R 4相同或不同并且表示氢，C 3和R 4相同或不同，表示氢，C C 1-10烷基，C 1-10 1-10烯基，C 3-8环烷基或芳基; 或-SR 5，其中R 5表示氢，C 1-10烷基，C 1-10 1-10烯基 ，芳基或C 3-8环烷基，基于通式（II）的三氟乙酰乙酸衍生物，其中R 1具有上述含义，通过微量 - 能够降低羰基官能团或通过所述微生物的无细胞酶提取物的有机体。

    Recombinantly producing levodione reductase
    63.
    发明授权
    Recombinantly producing levodione reductase 失效
    重组产左旋酮还原酶

    公开(公告)号：US06673583B2

    公开(公告)日：2004-01-06

    申请号：US10327726

    申请日：2002-12-23

    申请人： Sakayu Shimizu ,  Masaru Wada

    发明人： Sakayu Shimizu ,  Masaru Wada

    IPC分类号： C12P724

    CPC分类号： C12N9/0008 ,  C12P7/26

    摘要： The present invention is directed to genetic material useful for the preparation of actinol, such as an isolated DNA including a nucleotide sequence coding for an enzyme having levodione reductase activity, a polypeptide encoded by such a DNA, recombinant organisms, and the like. These genetic materials may originate from Corynebacterium, Cellulomonas, Planococcus, Arthrobacter, and the like. The present invention also provides a process for the production of actinol.

    摘要翻译： 本发明涉及可用于制备锕系元素的遗传物质，例如分离的DNA，包括编码具有左旋体还原酶活性的酶的核苷酸序列，由这种DNA编码的多肽，重组生物等。 这些遗传物质可以来自棒状杆菌属，丝杆菌属，球菌属，节杆菌属等。 本发明还提供了制备肌动蛋白的方法。

    Process for producing omega-9 highly unsaturated fatty acid and lipid containing the same
    65.
    发明授权
    Process for producing omega-9 highly unsaturated fatty acid and lipid containing the same 失效
    生产ω-9高度不饱和脂肪酸和含有它的脂质的方法

    公开(公告)号：US6150144A

    公开(公告)日：2000-11-21

    申请号：US917230

    申请日：1997-08-25

    申请人： Kengo Akimoto ,  Hiroshi Kawashima ,  Sakayu Shimizu

    发明人： Kengo Akimoto ,  Hiroshi Kawashima ,  Sakayu Shimizu

    IPC分类号： C12P7/64 ,  C12R1/645

    CPC分类号： C12P7/6427 ,  C12P7/6472

    摘要： The present invention discloses a process for producing lipid containing omega-9 highly unsaturated fatty acid by culturing in a medium a mutant strain obtained by mutation on a microorganism having the ability to produce arachidonic acid belonging to the genus Mortierella and so forth, in which .DELTA.12 desaturation activity is decreased or lost, but at least one of .DELTA.5 desaturation activity, .DELTA.6 desaturation activity and chain length elongation activity is elevated. Moreover, the present invention also discloses a process for producing omega-9 highly unsaturated fatty acid by collecting omega-9 highly unsaturated fatty acid from the culture or lipid described above.

    摘要翻译： 本发明公开了一种生产含有ω-9高度不饱和脂肪酸的脂质的方法，该方法是在培养基中培养由具有产生属于高山被孢霉属属的花生四烯酸的能力的微生物上突变获得的突变株，其中DELTA 12去饱和活性降低或丧失，但DELTA5去饱和活性，DELTA 6去饱和活性和链长伸长活性中的至少一种升高。 此外，本发明还公开了通过从上述培养物或脂质中收集ω-9高度不饱和脂肪酸来生产ω-9高度不饱和脂肪酸的方法。

    Regulatory gene for the expression of nitrile hydratase gene
    67.
    发明授权
    Regulatory gene for the expression of nitrile hydratase gene 失效
    用于表达腈水合酶基因的调控基因

    公开(公告)号：US5683913A

    公开(公告)日：1997-11-04

    申请号：US537434

    申请日：1995-10-02

    申请人： Sakayu Shimizu ,  Michihiko Kobayashi

    发明人： Sakayu Shimizu ,  Michihiko Kobayashi

    IPC分类号： C12N15/09 ,  C07K14/195 ,  C12N1/21 ,  C12N9/78 ,  C12N9/88 ,  C12N15/31 ,  C12N15/55 ,  C12N15/74 ,  C12P21/02 ,  C12R1/01 ,  C12N1/00 ,  C12N5/10 ,  C12N15/63

    CPC分类号： C12N9/78 ,  C07K14/195 ,  C12N15/74

    摘要： The invention relates to a regulatory gene coding for a polypeptide having the ability to activate a promoter for a nitrile hydratase gene, a recombinant DNA containing said regulatory gene, and a transformant transformed with said recombinant DNA. The introduction of the regulatory gene of the invention along with a nitrile hydratase gene and its promoter region permits bacteria of the genus Rhodococcus to produce a higher level of nitrile hydratase. Other extraneous gene can also be introduced into a region downstream of the promoter to produce other proteins in high yield.

    摘要翻译： 本发明涉及编码具有激活腈水合酶基因启动子能力的多肽的调节基因，含有所述调节基因的重组DNA和用所述重组DNA转化的转化体。 引入本发明的调节基因以及腈水合酶基因及其启动子区域使得红球菌属的细菌产生更高水平的腈水合酶。 其他外来基因也可以引入到启动子下游的区域，以高产率产生其它蛋白质。

    Method for producing S-adenosyl-L-homocysteine hydrolase
    69.
    发明授权
    Method for producing S-adenosyl-L-homocysteine hydrolase 失效
    S-腺苷-L-高半胱氨酸水解酶的制备方法

    公开(公告)号：US4609626A

    公开(公告)日：1986-09-02

    申请号：US634208

    申请日：1984-07-25

    申请人： Hideaki Yamada ,  Sakayu Shimizu ,  Shozo Shiozaki

    发明人： Hideaki Yamada ,  Sakayu Shimizu ,  Shozo Shiozaki

    IPC分类号： C12N9/14 ,  C12R1/01 ,  C12R1/05 ,  C12R1/06 ,  C12R1/065 ,  C12R1/13 ,  C12R1/15 ,  C12R1/20 ,  C12R1/265 ,  C12R1/29 ,  C12R1/365 ,  C12R1/38 ,  C12R1/465 ,  C12R1/64 ,  C12R1/645 ,  C12R1/32

    CPC分类号： C12N9/14 ,  Y10S435/822 ,  Y10S435/829 ,  Y10S435/83 ,  Y10S435/831 ,  Y10S435/843 ,  Y10S435/85 ,  Y10S435/863 ,  Y10S435/867 ,  Y10S435/872 ,  Y10S435/874 ,  Y10S435/886

    摘要： A method for producing S-adenosyl-L-homocysteine hydrolase, which comprises cultivating a microorganism having the ability to produce S-adenosyl-L-homocysteine hydrolase within its cells in a nutrient medium to accumulate said hydrolase in the cells, said microorganism being a bacterium belonging to the genera Alcaligenes, Pseudomonas, Acinetobacter, Arthrobacter, Enterobacter, Rhodopseudomonas, Agrobacterium, Micrococcus, Corynebacterium, Brevibacterium, Chromobacterium, Xanthomonas, Flavobacterium, Cellulomonas, Azotobacter and Protaminobacter, or an actinomycete belonging to the genera Streptomyces, Mycobacterium, Nocardia, Streptoverticillium, Micromonospora, Micropolyspora, Streptosporangium and Microellobosporia; and then recovering S-adenosyl-L-homocysteine hydrolase from the cells.

    摘要翻译： 一种S-腺苷-L-高半胱氨酸水解酶的制造方法，其特征在于，在营养培养基中培养具有在其细胞内产生S-腺苷-L-高半胱氨酸水解酶的能力的微生物，以在细胞中积聚所述水解酶，所述微生物为 属于产碱杆菌属，假单胞菌属，不动杆菌属，节杆菌属，肠杆菌属，红假单胞菌属，土壤杆菌属，微球菌属，棒状杆菌属，短杆菌属，色杆菌属，黄单胞菌属，黄杆菌属，单核细菌属，固氮菌属和Protaminobacter属的细菌或属于链霉菌属，分枝杆菌属，诺卡氏菌属， 链霉菌，小单孢菌，微孢子虫，链球菌属和微球菌属; 然后从细胞中回收S-腺苷-L-高半胱氨酸水解酶。