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公开(公告)号：US20180298331A1
公开(公告)日：2018-10-18
申请号：US15955335
申请日：2018-04-17
发明人： S. Jordan Kerns , Norman Wen , Carolina Lucchesi , Christopher David Hinojosa , Jacob Fraser , Geraldine Hamilton , Gad Vatine , Samuel Sances , Clive Svendsen , Daniel Levner , Dhruv Sareen
摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.
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公开(公告)号：US20180017586A1
公开(公告)日：2018-01-18
申请号：US15648182
申请日：2017-07-12
申请人： Emulate Inc. , Janssen Biotech, Inc
发明人： Daniel Levner , Christopher David Hinojosa , Norman Wen , Jacob Fraser , Justin Nguyen , Riccardo Barrile , Geraldine Hamilton , Catherine Karalis , Hyoung Shin Park , Antonio Varone , Andries Va der Meer , Monica Otieno , David Conegliano
CPC分类号： G01N33/86 , B01L3/5023 , B01L3/502707 , B01L3/502715 , B01L3/502746 , B01L3/502761 , B01L2200/02 , B01L2200/027 , B01L2200/0605 , B01L2200/0647 , B01L2200/16 , B01L2300/0809 , B01L2300/0816 , B01L2300/0867 , B01L2300/0883 , B01L2300/0887 , B01L2300/14 , B01L2300/16 , C12M23/16 , C12M29/00 , C12M29/04 , C12M29/10 , C12M35/08 , C12M41/46 , G01N33/54366 , G01N2500/10
摘要： Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.
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公开(公告)号：US20180017585A1
公开(公告)日：2018-01-18
申请号：US15648162
申请日：2017-07-12
申请人： Emulate Inc. , Janssen Biotech. Inc
发明人： Daniel Levner , Christopher David Hinojosa , Norman Wen , Jacob Fraser , Justin Nguyen , Riccardo Barrile , Geraldine Hamilton , Catherine Karalis , Hyoung Shin Park , Antonio Varone , Andries Van der Meer , Monica Otieno , David Conegliano
CPC分类号： G01N33/86 , B01L3/5023 , B01L3/502707 , B01L3/502715 , B01L3/502746 , B01L3/502761 , B01L2200/02 , B01L2200/027 , B01L2200/0605 , B01L2200/0647 , B01L2200/16 , B01L2300/0809 , B01L2300/0816 , B01L2300/0867 , B01L2300/0883 , B01L2300/0887 , B01L2300/14 , B01L2300/16 , C12M23/16 , C12M29/00 , C12M29/04 , C12M29/10 , C12M35/08 , C12M41/46 , G01N33/54366 , G01N2500/10
摘要： Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.
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公开(公告)号：US20180016535A1
公开(公告)日：2018-01-18
申请号：US15647762
申请日：2017-07-12
申请人： EMULATE, Inc.
发明人： Daniel Levner , Josiah Daniel Sliz , Christopher David Hinojosa , Joshua Gomes , Kyung Jin Jang
摘要： Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.
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公开(公告)号：US20170058248A1
公开(公告)日：2017-03-02
申请号：US15248690
申请日：2016-08-26
申请人： EMULATE, Inc.
CPC分类号： C12M23/16 , A01N1/021 , A01N1/0247 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L2200/025 , B01L2200/027 , B01L2200/12 , B01L2300/0681 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/161 , B01L2300/165 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , C12M21/08 , C12M23/38 , C12M23/40 , C12M23/42 , C12M29/10 , C12M35/04 , C12M41/40 , C12M41/48 , C12N5/0602 , C12N2521/00
摘要： A perfusion manifold assembly is described that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body.
摘要翻译： 可以设想一种培养模块，其允许一个或多个微流体装置的灌注和任选的机械致动，例如包含模拟体内器官的至少一个功能的细胞的片上微器件微流体装置。
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公开(公告)号：US11999932B2
公开(公告)日：2024-06-04
申请号：US17036652
申请日：2020-09-29
申请人： EMULATE, Inc.
摘要： The present invention is related to the field of fluidic devices, and in particular, microfluidic cell culture systems. The present invention provides pumping, recirculation and sampling for a microfluidic device or devices. The present invention provides solutions to the control of microfluidics for both terrestrial and space applications, including the control over the movement of fluids in zero gravity or microgravity.
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公开(公告)号：US11859165B2
公开(公告)日：2024-01-02
申请号：US18097755
申请日：2023-01-17
申请人： EMULATE, INC.
发明人： Daniel Levner , Christopher David Hinojosa , Norman Wen , Antonio Varone , Justin Nguyen , Lina Williamson , S. Jordan Kerns , Catherine Karalis , Geraldine Hamilton , Carol Lucchesi
IPC分类号： C12M3/06 , C12M1/00 , C12M1/12 , C12M1/42 , G01N33/50 , G01N33/543 , C12N5/0793 , C12N5/079 , C12N5/071 , C12N5/077
CPC分类号： C12M35/04 , C12M23/16 , C12M23/38 , C12M25/02 , C12M35/08 , C12N5/069 , C12N5/0619 , C12N5/0622 , C12N5/0629 , C12N5/0656 , C12N5/0688 , C12N5/0697 , C12N5/0698 , G01N33/50 , G01N33/5082 , G01N33/54366 , C12N2501/165
摘要： A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.
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公开(公告)号：US11834641B2
公开(公告)日：2023-12-05
申请号：US17950422
申请日：2022-09-22
申请人： EMULATE, Inc
发明人： Daniel Levner , Josiah Daniel Sliz , Christopher David Hinojosa , Joshua Gomes , Jose Fernandez-Alcon
IPC分类号： C12M3/06 , B01L3/00 , C12M1/00 , C12M1/42 , C12M1/34 , C12N5/071 , A01N1/02 , C12M3/00 , C12M1/36 , B01L9/00
CPC分类号： C12M23/16 , A01N1/021 , A01N1/0247 , B01L3/50273 , B01L3/502707 , B01L3/502715 , B01L3/502738 , B01L9/527 , C12M21/08 , C12M23/38 , C12M23/40 , C12M23/42 , C12M29/10 , C12M35/04 , C12M41/40 , C12M41/48 , C12N5/0602 , B01L2200/025 , B01L2200/027 , B01L2200/12 , B01L2300/0681 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/161 , B01L2300/165 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , C12N2521/00
摘要： A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.
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公开(公告)号：US20230287324A1
公开(公告)日：2023-09-14
申请号：US18097755
申请日：2023-01-17
申请人： EMULATE, INC.
发明人： Daniel Levner , Christopher David Hinojosa , Norman Wen , Antonio Varone , Justin Nguyen , Lina Williamson , S. Jordan Kerns , Catherine Karalis , Geraldine Hamilton , Carol Lucchesi
IPC分类号： C12M1/42 , C12M3/06 , C12M1/12 , G01N33/50 , G01N33/543 , C12M1/00 , C12N5/0793 , C12N5/079 , C12N5/071 , C12N5/077
CPC分类号： C12M35/04 , C12M23/16 , C12M23/38 , C12M25/02 , C12M35/08 , C12N5/0619 , C12N5/0622 , C12N5/0629 , C12N5/0656 , C12N5/0688 , C12N5/069 , C12N5/0697 , C12N5/0698 , G01N33/50 , G01N33/5082 , G01N33/54366 , C12N2501/165
摘要： A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.
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公开(公告)号：US11326149B2
公开(公告)日：2022-05-10
申请号：US16051004
申请日：2018-07-31
发明人： S. Jordan Kerns , Norman Wen , Carol Lucchesi , Christopher David Hinojosa , Jacob Fraser , Jefferson Puerta , Geraldine Hamilton , Robert Barrett , Clive Svendsen , Daniel Levner , Stephen R Targan , Michael Workman , Dhruv Sareen , Uthra Rajamani , Magdalena Kasendra
摘要： Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.
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