公开(公告)号：US11542476B2

公开(公告)日：2023-01-03

申请号：US16692383

申请日：2019-11-22

申请人： EMULATE, INC.

发明人： Remi Villenave ,  Carolina Lucchesi ,  Justin Nguyen ,  Catherine Karalis ,  Geraldine Hamilton ,  Buket Baddal ,  Michael Salmon

IPC分类号： C12N5/071 ,  B01L3/00 ,  C12N1/20

摘要： The present invention relates to microfluidic fluidic systems and methods for the in vitro modeling diseases of the lung and small airway. In one embodiment, the invention relates to a system for testing responses of a microfluidic Small Airway-on-Chip infected with one or more infectious agents (e.g. respiratory viruses) as a model of respiratory disease exacerbation (e.g. asthma exacerbation). In one embodiment, this disease model on a microfluidic chip allows for a) the testing of anti-inflammatory and/or anti-viral compounds introduced into the system, as well as b) the monitoring of the participation, recruitment and/or movement of immune cells, including the transmigration of cells. In particular, this system provides, in one embodiment, an in-vitro platform for modeling severe asthma as “Severe Asthma-on-Chip.” In some embodiments, this invention provides a model of viral-induced asthma in humans for use in identifying potentially effective treatments.

公开(公告)号：US20210214670A1

公开(公告)日：2021-07-15

申请号：US17160617

申请日：2021-01-28

申请人： Emulate, Inc.

发明人： Daniel Levner ,  Christopher David Hinojosa ,  Norman Wen ,  Antonio Varone ,  Justin Nguyen ,  Lina Williamson ,  S. Jordan Kerns ,  Catherine Karalis ,  Geraldine Hamilton ,  Carol Lucchesi

IPC分类号： C12M1/42 ,  C12M3/06 ,  C12M1/12 ,  G01N33/50 ,  G01N33/543 ,  C12M1/00 ,  C12N5/0793 ,  C12N5/079 ,  C12N5/071 ,  C12N5/077

摘要： A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.

公开(公告)号：US20230287355A1

公开(公告)日：2023-09-14

申请号：US18083916

申请日：2022-12-19

申请人： EMULATE, INC.

发明人： Remi Villenave ,  Carolina Lucchesi ,  Justin Nguyen ,  Catherine Karalis ,  Geraldine Hamilton ,  Buket Baddal ,  Michael Salmon

IPC分类号： C12N5/071 ,  B01L3/00 ,  C12N1/20

CPC分类号： C12N5/0688 ,  B01L3/502769 ,  C12N1/20 ,  C12N2502/00

摘要： The present invention relates to microfluidic fluidic systems and methods for the in vitro modeling diseases of the lung and small airway. In one embodiment, the invention relates to a system for testing responses of a microfluidic Small Airway-on-Chip infected with one or more infectious agents (e.g. respiratory viruses) as a model of respiratory disease exacerbation (e.g. asthma exacerbation). In one embodiment, this disease model on a microfluidic chip allows for a) the testing of anti-inflammatory and/or anti-viral compounds introduced into the system, as well as b) the monitoring of the participation, recruitment and/or movement of immune cells, including the transmigration of cells. In particular, this system provides, in one embodiment, an in-vitro platform for modeling severe asthma as “Severe Asthma-on-Chip.” In some embodiments, this invention provides a model of viral-induced asthma in humans for use in identifying potentially effective treatments.

公开(公告)号：US10908171B2

公开(公告)日：2021-02-02

申请号：US15648000

申请日：2017-07-12

申请人： Emulate Inc.

发明人： Daniel Levner ,  Christopher David Hinojosa ,  Norman Wen ,  Jacob Fraser ,  Justin Nguyen ,  Riccardo Barrile ,  Geraldine Hamilton ,  Catherine Karalis ,  Hyoungshin Park ,  Antonio Varone ,  Andries Van der Meer ,  Monicah Otieno ,  David Conegliano

IPC分类号： G01N33/86 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/42 ,  C12M1/34 ,  G01N33/543 ,  G01N33/80

摘要： Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.

公开(公告)号：US20180320125A1

公开(公告)日：2018-11-08

申请号：US15781370

申请日：2016-12-02

申请人： EMULATE, INC.

发明人： Daniel Levner ,  Christopher David Hinojosa ,  Norman Wen ,  Antonio Varone ,  Justin Nguyen ,  Lina Williamson ,  S. Jordan Kerns ,  Katia Karalis ,  Geraldine Hamilton ,  Carol Lucchesi

IPC分类号： C12M1/42 ,  C12M1/12 ,  C12M3/06 ,  C12N5/071 ,  C12N5/077 ,  C12M1/00 ,  C12N5/0793 ,  C12N5/079 ,  G01N33/50

CPC分类号： C12M35/04 ,  C12M23/16 ,  C12M23/38 ,  C12M25/02 ,  C12M35/08 ,  C12N5/0619 ,  C12N5/0622 ,  C12N5/0629 ,  C12N5/0656 ,  C12N5/0688 ,  C12N5/069 ,  C12N5/0697 ,  C12N5/0698 ,  C12N2501/165 ,  G01N33/50 ,  G01N33/5082 ,  G01N33/54366

摘要： A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.

公开(公告)号：US20180017586A1

公开(公告)日：2018-01-18

申请号：US15648182

申请日：2017-07-12

申请人： Emulate Inc. ,  Janssen Biotech, Inc

发明人： Daniel Levner ,  Christopher David Hinojosa ,  Norman Wen ,  Jacob Fraser ,  Justin Nguyen ,  Riccardo Barrile ,  Geraldine Hamilton ,  Catherine Karalis ,  Hyoung Shin Park ,  Antonio Varone ,  Andries Va der Meer ,  Monica Otieno ,  David Conegliano

IPC分类号： G01N33/86 ,  B01L3/00

CPC分类号： G01N33/86 ,  B01L3/5023 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/02 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/16 ,  C12M23/16 ,  C12M29/00 ,  C12M29/04 ,  C12M29/10 ,  C12M35/08 ,  C12M41/46 ,  G01N33/54366 ,  G01N2500/10

摘要： Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.

公开(公告)号：US20180017585A1

公开(公告)日：2018-01-18

申请号：US15648162

申请日：2017-07-12

申请人： Emulate Inc. ,  Janssen Biotech. Inc

发明人： Daniel Levner ,  Christopher David Hinojosa ,  Norman Wen ,  Jacob Fraser ,  Justin Nguyen ,  Riccardo Barrile ,  Geraldine Hamilton ,  Catherine Karalis ,  Hyoung Shin Park ,  Antonio Varone ,  Andries Van der Meer ,  Monica Otieno ,  David Conegliano

IPC分类号： G01N33/86 ,  B01L3/00

CPC分类号： G01N33/86 ,  B01L3/5023 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/02 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/16 ,  C12M23/16 ,  C12M29/00 ,  C12M29/04 ,  C12M29/10 ,  C12M35/08 ,  C12M41/46 ,  G01N33/54366 ,  G01N2500/10

摘要： Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.

公开(公告)号：US11248203B2

公开(公告)日：2022-02-15

申请号：US17160617

申请日：2021-01-28

申请人： EMULATE, INC.

发明人： Daniel Levner ,  Christopher David Hinojosa ,  Norman Wen ,  Antonio Varone ,  Justin Nguyen ,  Lina Williamson ,  S. Jordan Kerns ,  Catherine Karalis ,  Geraldine Hamilton ,  Carol Lucchesi

IPC分类号： C12M3/06 ,  C12M1/12 ,  C12M1/00 ,  C12M1/42 ,  G01N33/50 ,  G01N33/543 ,  C12N5/0793 ,  C12N5/079 ,  C12N5/071 ,  C12N5/077

摘要： A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.

公开(公告)号：US10989721B2

公开(公告)日：2021-04-27

申请号：US15648162

申请日：2017-07-12

申请人： Emulate Inc.

发明人： Daniel Levner ,  Christopher David Hinojosa ,  Norman Wen ,  Jacob Fraser ,  Justin Nguyen ,  Riccardo Barrile ,  Geraldine Hamilton ,  Catherine Karalis ,  Hyoungshin Park ,  Antonio Varone ,  Andries Van der Meer ,  Monicah Otieno ,  David Conegliano

IPC分类号： G01N33/86 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/42 ,  C12M1/34 ,  G01N33/543 ,  G01N33/80

摘要： Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.

公开(公告)号：US10961496B2

公开(公告)日：2021-03-30

申请号：US15781370

申请日：2016-12-02

申请人： EMULATE, INC.

发明人： Daniel Levner ,  Christopher David Hinojosa ,  Norman Wen ,  Antonio Varone ,  Justin Nguyen ,  Lina Williamson ,  S. Jordan Kerns ,  Catherine Karalis ,  Geraldine Hamilton ,  Carol Lucchesi

IPC分类号： C12M1/42 ,  C12M3/06 ,  C12M1/12 ,  G01N33/50 ,  G01N33/543 ,  C12M1/00 ,  C12N5/0793 ,  C12N5/079 ,  C12N5/071 ,  C12N5/077

摘要： A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.