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    Initial rate photometric method for immunoassay
    62.
    发明授权
    Initial rate photometric method for immunoassay 失效
    免疫测定的初始速率光度法

    公开(公告)号：US5583055A

    公开(公告)日：1996-12-10

    申请号：US301236

    申请日：1994-09-06

    申请人： Chan S. Oh ,  Elizabeth K. Kearns

    发明人： Chan S. Oh ,  Elizabeth K. Kearns

    IPC分类号： G01N21/25 ,  G01N21/27 ,  G01N21/75 ,  G01N33/53 ,  G01N33/536 ,  G01N33/557

    CPC分类号： G01N33/557 ,  G01N21/272

    摘要： An initial rate photometric immunoassay method useful for detecting and quantifying analytes in various physiological fluids is disclosed. The method is carried out by combining in a liquid medium an undiluted sample of analyte-containing physiological fluid, such as serum, and an excess of an anti-analyte antibody. A substantially constant initial rate of increase of the liquid medium's turbidity, due to the resulting immunoprecipitation reaction, is measured in real time and compared to a calibration curve prepared from known analyte concentrations to detect and quantify the analyte. Detection and quantification of numerous analytes including, haptens, drugs and proteins, in a wide variety of physiological fluids can be rapidly carried out by the present method.

    摘要翻译： 公开了用于检测和定量各种生理液体中的分析物的初始速率光度测定方法。 该方法通过在液体培养基中结合未稀释的含分析物的生理液体样品（例如血清）和过量的抗分析物抗体来进行。 由于所得的免疫沉淀反应，实时测量液体介质的浊度的基本恒定的初始增加速率，并与从已知分析物浓度制备的校准曲线进行比较，以检测和定量分析物。 通过本方法可以快速地进行多种生理液体中的多种分析物的检测和定量，包括半抗原，药物和蛋白质。

    Measuring apparatus
    63.
    发明授权
    Measuring apparatus 失效
    测量装置

    公开(公告)号：US5231576A

    公开(公告)日：1993-07-27

    申请号：US578577

    申请日：1990-09-07

    申请人： Yoshiro Suzuki ,  Noriyuki Kurihara

    发明人： Yoshiro Suzuki ,  Noriyuki Kurihara

    IPC分类号： G01N21/75 ,  G01N21/27 ,  G01N21/78 ,  G01N21/86

    CPC分类号： G01N21/8483 ,  G01N21/272 ,  G01N21/78 ,  G01N2201/1211

    摘要： The present invention is an apparatus for measuring the constituent concentration of a specimen on the basis of the color change in a test material housed in a test piece, which has reacted because of the specimen. The apparatus irradiates the test material which is a test piece loaded in the apparatus's main body and detects the intensity of the light reflected by the test material. A detection signal detected in the above way is sampled in a time period before and after the measurement timing at which the test material changes in color because of the specimen. An average value of the sampled detection signals is determined. The constituent concentration of the specimen is computed by referring to a stored conversion table on the basis of the average value determined in the above way.

    Solid-state optical assay imaging apparatus
    64.
    发明授权
    Solid-state optical assay imaging apparatus 失效
    固态光学测定成像装置

    公开(公告)号：US4737464A

    公开(公告)日：1988-04-12

    申请号：US780485

    申请日：1985-09-26

    申请人： Harden M. McConnell ,  Jonathan Briggs ,  John W. Parce

    发明人： Harden M. McConnell ,  Jonathan Briggs ,  John W. Parce

    IPC分类号： G01N21/25 ,  G01N21/27 ,  G01N21/00

    CPC分类号： G01N21/253 ,  G01N21/272 ,  G01N21/6428 ,  G01N2201/0461 ,  Y10S436/805 ,  Y10S436/807 ,  Y10T436/11

    摘要： An apparatus for determining a characteristic of a signal modulating component related to an analyte of interest at an assay site by measuring light modulated by the component. A light source uniformly illuminates the assay site and a reference site. The light is directed from the sites onto a group of memory cells on the surface of a random access memory. The memory cells will decay from a logical 1 to a logical 0 due to the light over a period of time which is a function of the intensity of the light and the reference voltage of the memory cells marking the difference between a 1 and a 0. The digital state of each of the memory cells in the group is measured a predetermined exposure time. The measured states of a group of memory cells corresponding to the assay site are compared to the memory cell states for a reference group. The measured states are then analyzed to determine a characteristic of the analyte.

    摘要翻译： 一种用于通过测量由该组分调制的光来确定在测定部位与感兴趣分析物相关的信号调节成分的特征的装置。 光源均匀地照射了测定部位和参考部位。 光从站点引导到随机存取存储器表面上的一组存储器单元上。 存储器单元将由于在一段时间内的光而从逻辑1衰减到逻辑0，该时间段是光的强度和记录单元的参考电压的函数，标记1和0之间的差。 在预定的曝光时间内测量组中的每个存储单元的数字状态。 将对应于测定位点的一组存储器单元的测量状态与参考组的存储器单元状态进行比较。 然后分析测量的状态以确定分析物的特征。

    Binary kinetic assay method and apparatus
    65.
    发明授权
    Binary kinetic assay method and apparatus 失效
    二元动力学测定方法和装置

    公开(公告)号：US4539295A

    公开(公告)日：1985-09-03

    申请号：US509903

    申请日：1983-06-30

    申请人： William M. Blough, Jr.

    发明人： William M. Blough, Jr.

    IPC分类号： C12Q1/00 ,  G01N21/27 ,  G01N33/70 ,  G01N21/77 ,  G01N33/00

    CPC分类号： G01N33/70 ,  C12Q1/00 ,  G01N21/272

    摘要： A kinetic assay method and apparatus for performing such method wherein a first sample containing predetermined concentrations of binary analytes is reacted with the reagent to form a reaction product. Reaction rates at first and second times are measured for the first reaction and a first ratio between such measured reaction rates is determined. A second reaction with a second sample and the reagent is also performed wherein the second sample contains predetermined concentrations of the binary analytes. Reaction rates at the first and second times are measured for the second reaction and a second ratio between such rates is determined. A third reaction with a third sample and the reagent is performed, the sample including unknown relative concentrations of the first and second analytes. Reaction rates are again measured at the first and second times and a third ratio between such measured reaction rates is determined.By comparing the first and third ratios, an indication can be provided to show that the reaction is not influenced by an interfering analyte. The third ratio may be compared to a range of ratios defined by the first and second ratios to determine the relative contribution of at least one of the analytes to a reaction product formed during the third reaction. The relative contribution of a selected one of the analytes may be used to determine a concentration of such analyte corrected for the influence or bias of the other analyte in the reaction.

    摘要翻译： 用于进行这种方法的动力学测定方法和装置，其中含有预定浓度的二元分析物的第一样品与试剂反应以形成反应产物。 在第一次反应中测量第一次和第二次的反应速率，并确定测量的反应速率之间的第一比率。 还进行与第二样品和试剂的第二反应，其中第二样品含有预定浓度的二元分析物。 在第二次反应中测量第一次和第二次的反应速率，并确定这种速率之间的第二比率。 进行与第三样品和试剂的第三反应，样品包括第一和第二分析物的未知相对浓度。 在第一次和第二次再次测量反应速率，并且测定这些测量的反应速率之间的第三比例。 通过比较第一和第三比例，可以提供指示来显示反应不受干扰分析物的影响。 可以将第三比率与由第一和第二比率限定的比率范围进行比较，以确定至少一种分析物对在第三反应期间形成的反应产物的相对贡献。 所选择的一种分析物的相对贡献可用于确定针对反应中其他分析物的影响或偏差校正的此类分析物的浓度。

    Apparatus for investigating fast chemical reactions by optical detection
    66.
    发明授权
    Apparatus for investigating fast chemical reactions by optical detection 失效
    用于通过光学检测来调查快速化学反应的装置

    公开(公告)号：US4074939A

    公开(公告)日：1978-02-21

    申请号：US686023

    申请日：1976-05-13

    申请人： Carl-Roland Rabl

    发明人： Carl-Roland Rabl

    IPC分类号： G01N21/03 ,  G01N21/27 ,  G01N21/59 ,  G01N21/64 ,  G01N21/24

    CPC分类号： G01N21/6445 ,  G01N21/0303 ,  G01N21/272 ,  G01N21/645 ,  G01N2021/0389 ,  G01N2021/0392 ,  G01N2021/1738 ,  G01N2021/6491 ,  G01N21/0332 ,  G01N21/59 ,  G01N2201/1226 ,  G01N2201/127

    摘要： An apparatus for investigating the course of fast chemical reactions in lid chemical systems, using optical detection and an external perturbation, especially by the temperature-jump method. The optical part of the apparatus comprises first and second light paths conveying a probing light beam and a sense light beam, respectively, and an optical lens system of extremely high aperture for measurements of absorption, fluorescence, and fluorescence polarization. The opto-electronic detection system comprises individual photodetector heads and a signal processing unit wherein special circuits for electronic division, zero-correction, large aperture error correction, and signal coupling eliminate disturbing signal components, reduce inherent errors of electronic signal processing, and simplify operation of the apparatus.

    摘要翻译： 一种用于研究液体化学系统中快速化学反应过程的设备，使用光学检测和外部扰动，特别是通过温度跳跃法。 该装置的光学部分包括分别传送探测光束和感测光束的第一和第二光路以及用于吸收，荧光和荧光偏振测量的极高孔径的光学透镜系统。 光电检测系统包括单独的光电检测器头和信号处理单元，其中用于电子分割，零校正，大孔径误差校正和信号耦合的特殊电路消除干扰信号分量，减少电子信号处理的固有误差，并简化操作 的装置。

    Apparatus for investigating fast chemical reactions by optical detection
    67.
    发明授权
    Apparatus for investigating fast chemical reactions by optical detection 失效
    用于通过光学检测来调查快速化学反应的装置

    公开(公告)号：US4054390A

    公开(公告)日：1977-10-18

    申请号：US686029

    申请日：1976-05-13

    申请人： Carl-Roland Rabl

    发明人： Carl-Roland Rabl

    IPC分类号： G01N21/03 ,  G01N21/27 ,  G01N21/59 ,  G01N21/64 ,  G01N21/24

    CPC分类号： G01N21/6445 ,  G01N21/272 ,  G01N21/645 ,  G01N2021/0389 ,  G01N2021/0392 ,  G01N2021/1738 ,  G01N2021/6491 ,  G01N21/0332 ,  G01N21/59 ,  G01N2201/1226 ,  G01N2201/127

    摘要： An apparatus for investigating the course of fast chemical reactions, which are initiated in a liquid chemical system under investigation by an external perturbation, e.g. a steep temperature rise (temperature jump). The apparatus comprises first and second light paths conveying a probing light beam and a sense light beam, respectively, and an optical system of extremely high aperture which allows a wide variety of types of measurements, including absorption, fluorescence, fluorescence polarization, and the like with a high signal-to-noise ratio even if small sample concentrations are used.

    摘要翻译： 一种用于调查快速化学反应过程的装置，其在通过外部扰动研究的液体化学系统中开始，例如， 温度升高（温度上升）。 该装置包括分别传送探测光束和感测光束的第一和第二光路以及极高孔径的光学系统，其允许多种类型的测量，包括吸收，荧光，荧光偏振等 即使使用小的样品浓度，也具有高的信噪比。

    Method of continuously determining the absorbance light of a chemical reaction mixture
    68.
    发明授权
    Method of continuously determining the absorbance light of a chemical reaction mixture 失效
    连续测定化学反应混合物吸收光的方法

    公开(公告)号：US3838926A

    公开(公告)日：1974-10-01

    申请号：US33454873

    申请日：1973-02-22

    申请人： FUJI PHOTO FILM CO LTD

    发明人： KATO H ,  OI N

    IPC分类号： G01J3/42 ,  G01N21/27 ,  G01N21/51 ,  G01N21/53 ,  G01N21/75 ,  G01N21/77 ,  G01N35/08 ,  G03D13/00 ,  G01N21/26 ,  G01J1/04

    CPC分类号： G01N21/53 ,  G01N21/272 ,  G03D13/007

    摘要： The progress of reaction in a turbid reaction mixture is continuously determined with high exactness by continuously determining the absorbance of the turbid reaction mixture passing through a flow cell enclosed in an integrating hollow globe, the inside surface of which reflects scattered light from the flow cell to eliminate the influence of the scattering of light due to the turbidity. This method is of value for continuous determination of the progress of a reaction especially in emulsion polymerizations and in the development of liquid photo emulsions for sensitometry. Apparatus to accomplish the method.

    摘要翻译： 连续测定浑浊反应混合物中的反应进程，通过连续测定通过封闭在集成中空球体中的流动池的混浊反应混合物的吸光度，其内表面反射来自流动池的散射光 消除由于浊度引起的光散射的影响。 该方法对于连续测定反应进程，特别是在乳液聚合中以及用于感光测定的液体光敏乳剂的开发中是有价值的。 完成该方法的装置。