公开(公告)号：US07608841B2

公开(公告)日：2009-10-27

申请号：US11451903

申请日：2006-06-12

Applicant: Taylor A. Reid ,  Roger H. Taylor ,  Kenneth J. Zajac

Inventor： Taylor A. Reid ,  Roger H. Taylor ,  Kenneth J. Zajac

IPC: G01N21/64

CPC classification number: G01J3/10 ,  G01J3/4406 ,  G01J2003/1213 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6484 ,  G01N2201/0446 ,  G01N2201/0612 ,  G01N2201/062

Abstract: A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source that emits an excitation light into an illumination tube; a plurality of collection optics located around an aperture in the illumination tube for collecting fluorescence; and a detector for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light from a light source into an illumination tube; directing the excitation light to an excitation filter; illuminating a sample with the excitation light to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics located around the illumination tube.

Abstract translation: 公开了具有不同光路的用于荧光激发和检测的系统和方法。 用于检测荧光的系统包括将激发光发射到照明管中的光源; 多个收集光学器件，位于照明管中用于收集荧光的孔周围; 以及用于确定荧光量的检测器。 检测荧光的方法包括将来自光源的激发光发射到照明管中; 将激发光引导到激发滤光器; 用激发光照射样品以产生发射光; 以及使用位于照明管周围的多个收集光学器件来检测发射光的光学特性。

公开(公告)号：US20080285025A1

公开(公告)日：2008-11-20

申请号：US12121120

申请日：2008-05-15

Applicant: Sakuichiro ADACHI

Inventor： Sakuichiro ADACHI

IPC: G01J3/00

CPC classification number: G01N21/253 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/0229 ,  G01J3/0262 ,  G01J3/04 ,  G01J3/36 ,  G01J3/42 ,  G01N21/13 ,  G01N21/255 ,  G01N2021/0325 ,  G01N2021/115 ,  G01N2021/3155 ,  G01N2201/0415 ,  G01N2201/0446 ,  G01N2201/0453 ,  G01N2201/0618

Abstract: A light amount is increased and an analyzing accuracy can be kept in accordance with an enlargement of a load angle, however, a scattered light tends to be loaded in an analysis accompanying the scattered light and a dynamic range of a concentration which can be measured becomes narrow. A light is dispersed by a light dispersing portion, a load angle of the received light is changed per wavelength, the load angle is made larger in the light of a wavelength having a small light amount, and the load angle is made smaller in the light a wavelength having a large light amount and used for an analysis accompanying a scattered light. Accordingly, it is possible to gain a dynamic range of a concentration which can be measured in the analysis accompanying the scattered light, while increasing the light amount and maintaining the analyzing accuracy.

Abstract translation: 光量增加，并且可以根据负载角的放大来保持分析精度，然而，伴随散射光的分析倾向于加载散射光，并且可测量的浓度的动态范围变为 狭窄。 通过光分散部分散射光，每波长改变接收光的负载角度，在具有小光量的波长的光中使负载角变大，并且使光的负载角变小 具有大光量的波长并用于伴随散射光的分析。 因此，在增加光量并保持分析精度的同时，可以获得在伴随散射光的分析中可以测量的浓度的动态范围。

公开(公告)号：US5738825A

公开(公告)日：1998-04-14

申请号：US854586

申请日：1997-05-12

Applicant: Helmut Rudigier ,  Roland Pfefferkorn ,  Kurt Tiefenthaler ,  Veronique Briquet ,  Alois Zetting ,  Peter Marbach ,  Francois Legay ,  Peter End

Inventor： Helmut Rudigier ,  Roland Pfefferkorn ,  Kurt Tiefenthaler ,  Veronique Briquet ,  Alois Zetting ,  Peter Marbach ,  Francois Legay ,  Peter End

IPC: G01N33/543 ,  B01L3/00 ,  G01N21/03 ,  G01N21/27 ,  G01N21/41 ,  G01N21/77 ,  G01N21/01

CPC classification number: G01N21/253 ,  B01L3/5085 ,  G01N21/7743 ,  B01L2300/0654 ,  G01N21/4788 ,  G01N2201/0446

Abstract: A detection cell which is used as a component of an optical biosensor comprises a transparent base plate and a sample plate on the base plate. The sample plate has a matrix of wells extending through it to each to receive a sample. The base plate includes a waveguiding film and a diffraction grating means to in couple an incident light field into the waveguiding film beneath a well to generate a diffracted light field to enable detection of a change in the effective refractive index of the waveguiding film.

Abstract translation: PCT No.PCT / EP94 / 02361 Sec。 371日期：1995年04月27日 102（e）日期1995年4月27日PCT 1994年7月18日PCT公布。 公开号WO95 / 03538 日期1995年2月2日作为光生物传感器的成分的检测单元，在基板上具有透明基板和样品板。 样品板具有通过其延伸穿过每个孔的孔的矩阵以接收样品。 基板包括波导膜和衍射光栅装置，用于将入射光场耦合到阱下面的波导膜中，以产生衍射光场，以便能够检测波导膜的有效折射率的变化。

公开(公告)号：US5265169A

公开(公告)日：1993-11-23

申请号：US775809

申请日：1991-10-11

Applicant: Masato Ohta ,  Yasuhiko Yokomori ,  Toshiyuki Furuta

Inventor： Masato Ohta ,  Yasuhiko Yokomori ,  Toshiyuki Furuta

IPC: G01N21/17 ,  G01N21/75 ,  G01N21/82 ,  G01N33/543 ,  G06K9/00

CPC classification number: G01N21/82 ,  G01N2021/755 ,  G01N2201/0407 ,  G01N2201/0446 ,  G01N2201/127

Abstract: A method for identifying the presence or absence of an aggregation of particles distributed on a translucent surface includes the steps of providing a light sensing device for sensing light incident thereon and producing an output value which represents the intensity of the incident light, and identifying a first output value which is produced by the light sensing device when there is no light incident thereon. The method also identifies a second output value which is produced by the light sensing means when, with no particles present on the translucent surface, light is directed through the translucent surface onto the light sensing device. With a distribution of particles deposited on the translucent surface, light is directed through the translucent surface and onto the light sensing device, causing the light sensing device to produce a third output value. A final output value is then determined as a function of said first, second and third output values.

Abstract translation: 用于识别分布在半透明表面上的颗粒的聚集的存在或不存在的方法包括以下步骤：提供用于感测入射在其上的光的光感测装置，并产生表示入射光强度的输出值，并且识别第一 当没有光入射时由光检测装置产生的输出值。 该方法还识别由光感测装置产生的第二输出值，当在半透明表面上没有颗粒存在时，光被引导通过透光表面到光感测装置上。 随着沉积在半透明表面上的颗粒的分布，光被引导通过半透明表面并且到达光感测装置，使光感测装置产生第三输出值。 然后确定最终输出值作为所述第一，第二和第三输出值的函数。

公开(公告)号：US5157455A

公开(公告)日：1992-10-20

申请号：US563648

申请日：1990-08-07

Applicant: James N. Macri ,  Edward J. Cook

Inventor： James N. Macri ,  Edward J. Cook

IPC: G01N21/27

CPC classification number: G01N21/274 ,  G01N2201/0446 ,  G01N2201/13

Abstract: An apparatus for the calibration and quality assurance of a multichannel spectrophotometer, particularly an ELISA spectrophotometer, comprises film selectively exposed in the presence of a color to produce a series of filters having a known first color and linearly increasing optical density. The response of the spectrophotometer is measured against the known color and linearly increasing optical density. Additional filters of at least one additional color permit checking the color response of the spectrophotometer. An algorithm determines whether the response conforms to predetermined conditions. An output is produced to provide a record of the calibration and quality assurance of the spectrophotometer. The invention has particular utility for conducting calibration and quality assurance of ELISA spectrophotometers used in clinical laboratory screening for infectious diseases, such as Hepatitis B. and the AIDS viruses.

Abstract translation: 用于多通道分光光度计，特别是ELISA分光光度计的校准和质量保证的装置包括在存在颜色的情况下选择性地曝光的膜以产生具有已知第一颜色和线性增加的光密度的一系列滤光片。 根据已知的颜色和线性增加的光密度测量分光光度计的响应。 至少一种额外的颜色的附加过滤器允许检查分光光度计的颜色响应。 算法确定响应是否符合预定条件。 产生输出以提供分光光度计的校准和质量保证的记录。 本发明特别用于进行临床实验室筛查感染性疾病如乙型肝炎和艾滋病病毒的ELISA分光光度计的校准和质量保证。

公开(公告)号：US5116765A

公开(公告)日：1992-05-26

申请号：US510883

申请日：1990-04-18

Applicant: Haruhisa Watanabe ,  Satoshi Tanaka ,  Shinya Matsuyama

Inventor： Haruhisa Watanabe ,  Satoshi Tanaka ,  Shinya Matsuyama

IPC: G01N21/82 ,  G01N33/53 ,  G01N35/00 ,  G01N35/02

CPC classification number: G01N33/5304 ,  G01N21/82 ,  G01N35/00 ,  G01N2201/0446 ,  G01N2201/0461 ,  G01N35/028

Abstract: A method for detecting and measuring the image of an agglutination particle pattern produced in a test liquid in which the existence of hemolysis in a test liquid is judged at the same time as an analysis is conducted of particle patterns produced in the test liquid in response to an immunological agglutination reaction. By this method, it is possible to increase the precision of the analysis of the particle pattern by feeding back the result of the judgement of the existence of hemolysis in the test liquid. A reaction apparatus suitable for use in the method according to the invention is also disclosed. In this apparatus, a plurality of wells for containing the test liquid are formed, and a plurality of marks are formed in positions where the marks can be read through the test liquid by an optical system for detecting the particle patterns produced in the test liquid. Therefore, hemolysis existing in the test liquid can be objectively judged.

Abstract translation: 用于检测和测量在测试液体中产生的凝集颗粒图案的图像，其中在与测试液体同时判断在测试液体中溶血的存在的同时进行响应于测试液体中产生的颗粒图案 免疫凝集反应。 通过该方法，可以通过反馈检测液中溶血存在的判断结果来提高粒子图案的分析精度。 还公开了适用于根据本发明的方法的反应装置。 在该装置中，形成容纳测试液体的多个孔，并且通过用于检测在测试液中产生的颗粒图案的光学系统通过测试液体读取标记的位置形成多个标记。 因此，可以客观地判断存在于测试液中的溶血。

公开(公告)号：US5035866A

公开(公告)日：1991-07-30

申请号：US155955

申请日：1988-02-16

Applicant: Jon C. Wannlund

Inventor： Jon C. Wannlund

IPC: B01L3/00 ,  C12M1/20 ,  G01N21/03 ,  G01N21/76

CPC classification number: B01L3/5025 ,  B01L3/50255 ,  C12M21/18 ,  C12M23/12 ,  G01N21/03 ,  G01N21/76 ,  G01N2021/0325 ,  G01N2021/0328 ,  G01N2021/0357 ,  G01N2021/1768 ,  G01N2201/0446 ,  Y10S436/809 ,  Y10T436/2575

Abstract: Apparatus for performing and measuring chemical reactions includes a reaction test apparatus having reaction wells wherein reactants are controllably mixed, and exposure apparatus which receives and positions the reaction test apparatus adjacent a photographic film. Each of the reaction wells includes at least two reaction cups, arranged one above the other. The uppermost reaction cups have orifices in the bottoms, so that liquid can be mixed and reacted in the uppermost cup, and then controllably transferred to the lower cup to be mixed with additional reactants. In a preferred embodiment, the reaction cups are supported in plates that are structurally integral with the cups, and are superimposed to make a test block. The test block is retained in the exposure apparatus, and liquid is forced from the upper cup to the lower cup by application of pressure to the top of the upper cup. The apparatus of the invention is particularly suited for measuring reactions that produce luminescence of short duration, as the reactants can be conveniently mixed in darkness, while the film is being exposed.

Abstract translation: 用于进行和测量化学反应的装置包括具有可反应混合反应物的反应孔的反应试验装置，以及将反应试验装置接收并定位在照相胶片附近的曝光装置。 每个反应孔包括至少两个反应杯，一个彼此排列。 最上面的反应杯在底部具有孔口，使得液体可以在最上面的杯子中混合和反应，然后可控制地转移到下部杯中以与另外的反应物混合。 在优选实施例中，反应杯被支撑在与杯子结构一体的板中，并叠加以制成测试块。 测试块被保留在曝光设备中，并且通过向上杯的顶部施加压力将液体从上杯强制到下杯。 本发明的装置特别适用于测量产生短持续时间的发光的反应，因为反射剂可以在黑暗中方便地混合，同时曝光该膜。

公开(公告)号：US4948564A

公开(公告)日：1990-08-14

申请号：US430812

申请日：1989-11-02

Applicant: David Root ,  George Lyman

Inventor： David Root ,  George Lyman

IPC: B01D29/01 ,  B01D61/18 ,  B01L3/00 ,  B01L9/06 ,  G01N21/03

CPC classification number: B01L9/06 ,  B01D61/18 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/50855 ,  G01N2021/0357 ,  G01N2201/0446 ,  Y10S436/809 ,  Y10T436/255

Abstract: A filter strip and composite assemblies for filtering microliter quantities of fluids. A linear array of wells having open top and bottom ends are connected by frangible webs in spaced-apart relation with discrete filter membranes closing the bottom ends of each well. Tabs are provided on the ends of the filter strip for holding the same and supporting the strip in a rectangular holder having alpha-numeric designations for identifying each well in a plurality of such filter strips contained within the holder. The filter strip may be used in a vacuum manifold for applying a pressure differential across the filter membrane and directing the filtrate into an aligned aperture of a closed bottom well of an array of wells held within the manifold. Alternatively, the filter strip may be used with a transfer plate for directing the filtrate from each well of the filter strip to an aligned well of a closed bottom array of wells. Another composite assembly includes a pressure manifold for applying an increased pressure above the membranes.

Abstract translation: 用于过滤微量流体的过滤条和复合组件。 具有开放的顶部和底部的井的线性阵列通过易碎的腹板以间隔开的关系连接，离心过滤膜封闭每个孔的底端。 在过滤条的端部设置有用于保持其的突片，并将条带支撑在具有字母数字标号的矩形保持器中，用于识别容纳在保持器内的多个这种过滤条中的每个孔。 过滤条可以用在真空歧管中，用于在过滤膜上施加压力差，并将滤液引导到保持在歧管内的阱阵列的封闭的底部井的对准孔。 或者，过滤条可以与转移板一起使用，用于将滤液从过滤条的每个孔导向孔的封闭底部阵列的对准井。 另一复合组件包括用于在膜上施加增加压力的压力歧管。

公开(公告)号：US4927265A

公开(公告)日：1990-05-22

申请号：US187789

申请日：1988-04-29

Applicant: Robert G. Brownlee

Inventor： Robert G. Brownlee

IPC: G01J3/18 ,  G01J3/28 ,  G01J3/42 ,  G01J3/44 ,  G01N21/64 ,  G01N27/447 ,  G01N35/00

CPC classification number: G01N21/31 ,  G01N21/64 ,  G01N27/44721 ,  G01N27/44756 ,  G01J3/1838 ,  G01J3/2803 ,  G01J3/42 ,  G01J3/4406 ,  G01N2021/1734 ,  G01N2021/6417 ,  G01N2021/6491 ,  G01N2035/00306 ,  G01N2035/00316 ,  G01N21/274 ,  G01N2201/0446

Abstract: Automated capillary electrophoresis tests can be performed using the capillary electrophoresis instrument of this invention. The capillary electrophoresis instrument has means for automatically purging and replenishing electrolytes, for automatically entering a sample into the capillary tube and for automatically performing capillary electrophoresis. A detector for simultaneous detection of absorbance and fluorescence provides means for quantifing the species separated by electrophoresis.

Abstract translation: 可以使用本发明的毛细管电泳仪进行自动毛细管电泳测试。 毛细管电泳仪具有自动清洗和补充电解质的装置，用于将样品自动进入毛细管并自动进行毛细管电泳。 用于同时检测吸光度和荧光的检测器提供了用于定量通过电泳分离的物种的手段。

公开(公告)号：US4810096A

公开(公告)日：1989-03-07

申请号：US36006

申请日：1987-04-08

Applicant: Andrew J. Russell ,  Colin Calvert

Inventor： Andrew J. Russell ,  Colin Calvert

IPC: G01N21/13 ,  G01N21/25 ,  G01N21/59 ,  G01N21/01

CPC classification number: G01N21/253 ,  G01N2201/0446

Abstract: A small, compact plate reader is disclosed for microtitre plates. The reader comprises a totally enclosed housing (19), means e.g. a drawer (2) for receiving and inserting into the housing a microtitre plate (7) which is received into the housing between a light source and a photodetector positioned in vertical alignment above and below the microtitre plate. The light source and detector are mounted on a carriage movable in the housing along each of two orthogonal axes by means of the manual control knobs (10,11) on either side of the housing. The light source and detector are thus positionable with each of the wells of the microtitre plate in turn enabling absorbancy measurements to be made, and visually displayed by a digital display (9). A window (8) in the housing (1) marked with indices corresponding to the cells in the microtitre plate permits a visual indication of the position of the detector head relative to the microtitre plate, for example, by illumination from below of the appropriate index mark by the light source itself, or by a secondary light source.

Abstract translation: 公开了一种用于微量滴定板的小型紧凑型读板器。 读取器包括全封闭壳体（19）， 抽屉（2），用于接收和插入微量滴定板（7），所述微量滴定板（7）被容纳在光源和位于微量滴定板上下垂直对准的光电检测器之间的壳体内。 光源和检测器通过壳体两侧的手动控制旋钮（10,11）安装在可沿两个正交轴线的壳体内移动的支架上。 因此，光源和检测器因此可以与微量滴定板的每个阱定位，从而使得能够进行吸光度测量，并且由数字显示器（9）可视地显示。 在壳体（1）中的窗口（8）中标有与微量滴定板中的单元相对应的指标，允许例如通过从适当指数的下方照射检测器头相对于微量滴定板的位置的视觉指示 由光源本身或二次光源标记。