公开(公告)号：US20240326012A1

公开(公告)日：2024-10-03

申请号：US18623906

申请日：2024-04-01

Applicant: Pierce Biotechnology, Inc.

Inventor： Ramesh Ganapathy ,  Christopher Etienne ,  Deven Etnyre ,  Ashok Salunkhe ,  Atul Deshpande

IPC: B01J20/24 ,  B01D15/34 ,  B01J20/30 ,  C07K1/14

CPC classification number: B01J20/24 ,  B01D15/34 ,  B01J20/3042 ,  C07K1/14

Abstract: The present specification relates to compositions, devices, apparatus, methods, kits and systems for sample preparation (e.g., separation, reduction or removal of small molecules from biomolecules in a sample). Exemplary small molecules that can be separated, reduced or removed have a molecular weight range of

公开(公告)号：US12071468B2

公开(公告)日：2024-08-27

申请号：US17688588

申请日：2022-03-07

Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED

Inventor： Meinhard Hasslacher ,  Martin Feichtinger ,  Philipp M. Bärnthaler ,  Christa Mayer ,  Birgit Reipert ,  Mantas Malisauskas ,  Julia Anzengruber

IPC: C07K14/755 ,  A61K38/00 ,  B01D15/32 ,  B01D15/34 ,  B01D15/36 ,  C07K1/16 ,  C07K1/18 ,  C07K1/36

CPC classification number: C07K14/755 ,  B01D15/327 ,  B01D15/34 ,  B01D15/363 ,  C07K1/16 ,  C07K1/18 ,  C07K1/36 ,  A61K38/00 ,  B01D15/363 ,  B01D15/34 ,  B01D15/363 ,  B01D15/327 ,  B01D15/34

Abstract: The present invention relates to a method for purifying a Factor VIII (FVIII) subspecies from a composition comprising FVIII, said method comprising an anion exchange chromatography step, a size exclusion chromatography step, and a concentration step. The invention also relates to a composition comprising a purified FVIII subspecies.

公开(公告)号：US20240198316A1

公开(公告)日：2024-06-20

申请号：US18556895

申请日：2022-05-30

Applicant: Suzhou Sepax Technologies, Inc.

Inventor： Huiming MAO ,  Feng XU ,  Ke YANG ,  Xinmei HU ,  Xueying HUANG

IPC: B01J20/288 ,  B01D15/20 ,  B01D15/32 ,  B01D15/34 ,  B01D15/36 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32

CPC classification number: B01J20/288 ,  B01D15/206 ,  B01D15/327 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01D15/3847 ,  B01J20/267 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28059 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/289 ,  B01J20/3064 ,  B01J20/3085 ,  B01J20/321 ,  B01J20/3221 ,  B01J20/3244 ,  B01J20/3293 ,  B01J2220/54

Abstract: A synthetic polymeric porous medium with a core-shell(s) hierarchical layer structure and has an essentially homogeneous porous structure from inside to outside of the medium, whose core and shell(s) are covalently modified with distinct chemical functional groups or same functional group with different density. Here the methodologies for resin syntheses and core-shell(s) modifications and liquid chromatographic applications of the newly developed resins in the field of analysis and purification of Tween surfactants, virus-like particles (VLP)/vaccines/viral vectors/viruses, antibody, and mRNA are disclosed.

公开(公告)号：US20240157334A1

公开(公告)日：2024-05-16

申请号：US18281095

申请日：2022-06-03

Applicant: Resonac Corporation

Inventor： Naoki UCHIYAMA ,  Yuzuru KOKIDO

IPC: B01J20/285 ,  B01D15/34 ,  B01J20/26 ,  B01J20/30

CPC classification number: B01J20/285 ,  B01D15/34 ,  B01J20/264 ,  B01J20/3085

Abstract: Provided are a packing material used for size exclusion chromatography, which has high alkali resistance and suppressed non-specific adsorption, and a method for producing the same. A packing material, wherein one end of a block copolymer represented by the following formula 1 is bonded to a porous organic polymer carrier containing 60 to 95 mol % of a repeating unit derived from glycidyl methacrylate and 5 to 40 mol % of a repeating unit derived from a polyfunctional monomer via an ether bond derived from a terminal hydroxyl group,


HO—(C2H4O)l—(C3H6O)m—(C2H4O)n—H  (formula 1)

wherein l is an integer of 5 to 140, m is an integer of 15 to 75, and n is an integer of 5 to 140, and a proportion of m to a sum of l, m, and n is 5 to 85%.

公开(公告)号：US11969670B2

公开(公告)日：2024-04-30

申请号：US17385874

申请日：2021-07-26

Applicant: Mark M. Kushnir ,  Alan L. Rockwood

Inventor： Mark M. Kushnir ,  Alan L. Rockwood

IPC: B01D15/34 ,  C07K1/16 ,  G01N33/538 ,  G01N33/74 ,  G01N33/82

CPC classification number: B01D15/34 ,  C07K1/16 ,  G01N33/538 ,  G01N33/74 ,  G01N33/82

Abstract: A method of separating free target analyte from protein-bound target analyte is described. Such can include obtaining an aqueous sample containing a target analyte in a free form (free target analyte) and the target analyte in a protein-bound form (protein-bound target analyte), passing the aqueous sample through a size exclusion chromatography matrix with a molecular weight cut off sufficient to allow the free target analyte to permeate into pores of the size exclusion chromatography matrix and exclude the protein-bound target analyte, whereupon the free target analyte adheres to and is immobilized by the size exclusion chromatography matrix and the protein-bound target analyte does not adhere to by the size exclusion chromatography matrix, separating the free target analyte from the protein-bound analyte by removing the protein-bound target analyte from the size exclusion chromatography matrix, and eluting the free target analyte from the size exclusion chromatography matrix with an organic solvent.

公开(公告)号：US20240131450A1

公开(公告)日：2024-04-25

申请号：US18547792

申请日：2022-02-24

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Kuan-Yin LIN ,  Andrew W. VAIL ,  Daniel J. O'NEAL ,  Masayuki NAKAMURA ,  Jonathan F. HESTER ,  Kannan DASHARATHI ,  Alexei M. VOLOSHIN

IPC: B01D15/34 ,  B01D15/22 ,  B01D15/36

CPC classification number: B01D15/34 ,  B01D15/22 ,  B01D15/36

Abstract: A charged depth filter for removing cells and/or cellular debris from a biopharma feedstock having a first functionalized nonwoven layer having a first calculated pore size and a first dynamic charge capacity; a second functionalized nonwoven layer having a second calculated pore size and a second dynamic charge capacity positioned after the first functionalized nonwoven layer in the direction of the biopharma feedstock flow, and wherein the first calculated pore size is greater than the second calculated pore size, and the first dynamic charge capacity is less than the second dynamic charge capacity.

公开(公告)号：US11951417B2

公开(公告)日：2024-04-09

申请号：US17231808

申请日：2021-04-15

Applicant: Waters Technologies Corporation

Inventor： Pamela C. Iraneta ,  Stephen James Shiner ,  Raymond P. Fisk ,  Lavelay Kizekai

IPC: B01D15/20 ,  B01D15/34 ,  B01D71/06 ,  G01N30/50

CPC classification number: B01D15/20 ,  B01D15/34 ,  B01D71/06 ,  G01N30/50

Abstract: This disclosure pertains to performance enhancing conditioning and storage solvents containing low levels of buffer and salt for reproducible and improved size exclusion chromatography (SEC). In some embodiments, the present disclosure pertains to storage solvents for protein-based size exclusion chromatography that include water, a water-miscible organic solvent, a phosphate salt, and a neutral salt. In some embodiments, the present disclosure pertains to columns for protein-based size exclusion chromatography that include porous particles and such a storage solvent.

公开(公告)号：US20230266284A1

公开(公告)日：2023-08-24

申请号：US18168865

申请日：2023-02-14

Applicant: Water Technologies Corporation

Inventor： Lavelay Kizekai ,  Stephen Shiner ,  Matthew Lauber

IPC: G01N30/72 ,  G01N30/14 ,  G01N30/88 ,  B01D15/34

CPC classification number: G01N30/7233 ,  B01D15/34 ,  G01N30/14 ,  G01N30/88 ,  G01N2030/027

Abstract: The present disclosure is directed to methods for performing size exclusion chromatographic (SEC) separations. Embodiments of the present disclosure feature methods for improved separations of biomolecule analytes, such as CRISPR-related proteins, nucleotides, and ribonucleoprotein complexes, in SEC, for example, by using hydroxy-terminated polyethylene glycol surface modified stationary phase materials and/or C2/PEG surface modified column hardware.

公开(公告)号：US20230143149A1

公开(公告)日：2023-05-11

申请号：US18094663

申请日：2023-01-09

Applicant: BioMadison, Inc.

Inventor： Francis Mark Dunning ,  Ward C. Tucker

IPC: C07K1/34 ,  G01N33/68 ,  B01D61/16 ,  B01D15/34 ,  B01D61/14

CPC classification number: C07K1/34 ,  G01N33/6803 ,  B01D61/16 ,  B01D15/34 ,  B01D61/145 ,  B01D2311/12

Abstract: Systems are described in which a primary detergent or surfactant in an aqueous solution is removed by the addition of a secondary detergent or surfactant in concentrations that exceed the critical micellar concentration (CMC) of the secondary detergent or surfactant using a size separation device. These systems are particularly applicable to protein-containing solutions. Typical primary detergents/surfactants include polysorbate 20, polysorbate 80, and Triton X-100. Suitable secondary detergents or surfactants can be ionic, nonionic, or zwitterionic. Typical secondary detergents/surfactants include, but are not limited to, galactoside detergents (e.g. octyl-β-galactoside), glucamide detergents (e.g. MEGA 8, MEGA 9, MEGA 10), cholamide detergents (e.g. CHAPS, CHAPSO, BIGCHAPS), and sulfobetaine detergents (such as sulfobetaine 3-10).

公开(公告)号：US11590433B2

公开(公告)日：2023-02-28

申请号：US16698211

申请日：2019-11-27

Applicant: Tetracore, Inc.

Inventor： William M. Nelson ,  Kyle Armantrout ,  David R. Almassian ,  Aymeric Randanne de Vazeille ,  Tracy Calvin Fecteau ,  Colin Kuserk

IPC: B01D15/14 ,  B01D15/42 ,  B01D15/22 ,  B01D15/02 ,  B01D15/20 ,  B01D15/34 ,  B01D15/18 ,  C12N15/10

Abstract: A method and system for solid phase extraction of a compound of interest from a sample matrix using a syringe having a barrel and a plunger, a sorbent for use with the syringe, and a desalting purification column having an end configured to receive liquid from the syringe body.